7 results about "Composite tissue" patented technology

The invention discloses a degradable macroporous composite tissue engineering scaffold, a structural performance regulation and control method and application, and belongs to the technical field of biomedical materials. Induced phase separation is realized through design of gel molecules and a solvent, so that a macroporous structure is formed; the pore structure is adjusted by controlling the proportion of the polyvinyl alcohol aqueous solution, the glycerol and the hydrophilic degradable biomacromolecule aqueous solution; the mechanical property of the stent is regulated and controlled by adding the degradable inorganic micro-nano particles; meanwhile, a pore structure and a scaffold material with spatial heterogeneity in mechanical property are constructed through hot melt interface integration. The problems that an existing tissue engineering scaffold is insufficient in controllability of a pore structure and mechanical properties and the like are solved, and the degradable macroporous composite tissue engineering scaffold material is suitable for being used as a degradable macroporous composite tissue engineering scaffold material for promoting collaborative injury repair of soft and hard tissues such as osteochondral tissues, muscular bones and tendon bones.

The invention belongs to the technical field of tissue engineering scaffolds, and relates to a degradable micro-nano fiber composite tissue engineering scaffold and a preparation method thereof.The degradable micro-nano fiber composite tissue engineering scaffold is composed of evenly-distributed micron-sized skeleton fibers and evenly-distributed bioactive nano-sized filling fibers, the diameter of the micron-sized skeleton fibers is 15-25 microns, and the diameter of the bioactive nano-sized filling fibers is 10-20 microns. The diameter difference between the micron-sized skeleton fibers and the bioactive nano-sized filling fibers is more than 50 times; the bioactive nanoscale filling fibers are distributed on the surfaces of the micron-sized skeleton fibers and among different micron-sized skeleton fibers, and the micron-sized skeleton fibers and the bioactive nanoscale filling fibers penetrate through each other to form a three-dimensional graded fiber network structure; during preparation, a spinning solution A and a spinning solution B are prepared respectively, and the degradable micro-nano fiber composite tissue engineering scaffold is prepared by adopting double nozzles to spray the solutions for electrostatic spinning. The degradable micro-nano fiber composite tissue engineering scaffold disclosed by the invention has a three-dimensional interpenetrating hierarchical network structure, long-acting mechanical supporting capability and good cell compatibility.

Provided herein are perfusate compositions, and methods and systems for using such composition to perfuse a vascularized composite tissue (e.g., limb, face, abdominal wall or flap) to preserve such composite tissues (e.g., for auto or allotransplant). In certain embodiments, the perfusate compositions comprise at least one of the following reagents: i) N-acetylcysteine; ii) pantothenate or pantothenic acid at a concentration of at least 15 micromol / liter in said composition; and / or iii) carnitine, as well as further comprising one of the following: i) at least one bicarbonate / CO2-dependent anaplerotic substrate, and at least one bicarbonate / CO2 buffer, or ii) at least one non-bicarbonate / CO2-dependent anaplerotic substrate. In certain embodiments, the perfusate compositions are free, or detectably free, of alpha amino acids.

The application provides a fiber-reinforced composite tissue repair material, characterized in that the repair material comprises a hydrogel matrix layer, a microsphere phase dispersed in the hydrogel matrix layer and a fiber-reinforced layer combined with the hydrogel matrix layer; wherein the hydrogel matrix layer comprises N-acrylated carboxymethyl chitosan and N-acrylated collagen; the microsphere phase is a drug-loaded GelMA microsphere, and the fiber-reinforced layer is an ordered fiber membrane prepared by electrospinning of collagen and alkali-degraded poly(lactic-co-glycolic acid) and has an ordered fiber membrane arranged in a single direction or a layer plate-like arrangement. The fiber-reinforced composite tissue repair material has good transparency, mechanical stability and sustained drug release capacity, can promote tissue cell adhesion, proliferation, directional arrangement and related phenotype expression, inhibit abnormal fibrosis tendency, promote epithelial coverage, and is beneficial to matrix repair and surface reconstruction.

This invention discloses a 3D printing bio-ink, a composite tissue engineering scaffold, and their 3D printing methods and applications. The 3D printing bio-ink comprises component A and component B; component A includes 500-1500 g / L β-tricalcium phosphate, 0.5-5 g / L collagen, 0-10 g / L graphene oxide, and 0.1-5 g / L chitosan and acetic acid; component B includes 100-400 g / L cellulose nanocrystals and water; the volume ratio of components A to B is (0.5-2):1. The 3D printing method for the composite tissue engineering scaffold includes the following steps: loading the 3D printing bio-ink into a 3D printer; designing and importing preset scaffold model parameters; setting the printhead specifications according to the ink properties; correcting the printing offset; determining the printing pressure and speed; setting the printhead temperature and printing platform temperature; forming the preset structure using direct-write printing; pre-drying in an oven followed by supercritical drying to obtain the composite tissue engineering scaffold. The composite tissue engineering scaffold of this invention has excellent biocompatibility and structural stability and can be used in the repair of jawbone defects and other oral tissue regeneration fields.

The invention relates to the field of in-vitro culture and induced differentiation of animal cells, in particular to a culture medium for rapid and synchronous myobiogenesis and adipogenesis serum-free co-induction of fish stem cells and an induction method. According to the method, a serum-free induction culture medium containing specific components is used, nutrition, metabolism and fatty acid delivery conditions required by muscle cell fusion and lipid droplet formation are met in the same induction system at the same time, the process complexity and batch-to-batch fluctuation caused by step-by-step induction are reduced, stable construction of the muscle-lipid composite tissue is achieved, and the method is suitable for large-scale popularization and application. Controllable supply and spectral pattern adjustment of key fatty acid components such as DHA and EPA are supported; synchronous differentiation is started when the cell fusion degree reaches the specified standard, and finally the fish-derived muscle-fat composite tissue with mature muscle fibers and fat droplets at the same time is obtained.

The application relates to a biological 3D printing cartilage-fiber-epithelium integrated tissue engineering trachea and a construction method and application thereof. First, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; epithelium specific biological ink is prepared by wrapping epithelial cells with matrix hydrogel; then, through a step-by-step assembly strategy, a C-shaped cartilage ring and a fiber ring are alternately printed and a ring-shaped tubular structure is formed by using a double-needle extrusion type biological 3D printing technology, and then a layer of epithelium specific biological ink is attached in the lumen to construct a tubular structure of cartilage-fiber-epithelium multi-tissue composite; it is verified that after being implanted subcutaneously in a naked mouse for 8 weeks, a relatively mature cartilage-vascularized fiber-epithelium tissue composite tissue engineering trachea can be regenerated, and a clinically transformable strategy is provided for the regeneration treatment of complex tracheal injury.