12 results about "Composite tissue" patented technology

The invention discloses a polymorphic graphite-carbide steel-based multiphase wear-resistant composite material and a preparation method and application thereof.The material is of a composite tissue structure of spheroidal graphite, three-dimensional coralline graphite, multiple carbides and a steel matrix, and the multiple carbides are M3C type carbides and MC type carbides. Comprising the core steps of component design, composite inoculant proportion design, smelting and inoculation treatment, subsequent heat treatment and the like, and is characterized in that synergistic precipitation of spheroidal graphite, three-dimensional coralline graphite and various and multi-size carbides is achieved through chemical component design and inoculation regulation and control; the invention further provides application of the material in high-impact, heavy-load or dry-friction working conditions of crushing or grinding equipment. The material provided by the invention has better comprehensive wear resistance under complex working conditions, has structural stability, impact resistance, lubricity and thermal stability, and has wide engineering application prospects in severe environments such as high impact, high load, dry friction and the like.

This invention relates to a composite tissue adhesive and its application in hemostasis, sterilization, and wound healing promotion. The adhesive comprises component A and component B. Component A includes: methacrylic anhydride-modified gelatin, dodecaldehyde-modified chitosan, polycaprolactone / methacrylic anhydride-modified gelatin blended nanofibers, a photosensitive crosslinking agent, and water. Component B is an aqueous solution of an amino crosslinking agent. In use, components A and B are mixed, injected into the wound, and irradiated with ultraviolet or blue light. The tissue adhesive of this invention is injected directly into the wound site in liquid form. Upon light irradiation, it forms a gel structure in situ with a strength comparable to that of skin, simultaneously providing hemostasis, sterilization, and wound healing promotion.

The invention discloses a degradable macroporous composite tissue engineering scaffold, a structural performance regulation and control method and application, and belongs to the technical field of biomedical materials. Induced phase separation is realized through design of gel molecules and a solvent, so that a macroporous structure is formed; the pore structure is adjusted by controlling the proportion of the polyvinyl alcohol aqueous solution, the glycerol and the hydrophilic degradable biomacromolecule aqueous solution; the mechanical property of the stent is regulated and controlled by adding the degradable inorganic micro-nano particles; meanwhile, a pore structure and a scaffold material with spatial heterogeneity in mechanical property are constructed through hot melt interface integration. The problems that an existing tissue engineering scaffold is insufficient in controllability of a pore structure and mechanical properties and the like are solved, and the degradable macroporous composite tissue engineering scaffold material is suitable for being used as a degradable macroporous composite tissue engineering scaffold material for promoting collaborative injury repair of soft and hard tissues such as osteochondral tissues, muscular bones and tendon bones.

The application discloses a YWHAH active polypeptide and a preparation method and application thereof, and belongs to the field of biological medicine and tissue engineering. The polypeptide sequence is LRDNLTLWTSDQQDE or TLWTSDQQDEEAGEG, which can target and block the GREM1 / YWHAH protein complex, and release the inhibition of GERM1 on the BMP signal pathway. Experiments prove that the polypeptide can significantly improve the osteogenic and dentinogenic differentiation ability of dental pulp mesenchymal stem cells, up-regulate the alkaline phosphatase activity and cell mineralization level, promote the expression of osteogenic and dentinogenic marker genes, and induce the formation of bone-like and dentin-like new tissues in vivo. The polypeptide has the advantages of small molecular weight, convenient chemical synthesis, good physicochemical stability, weak immunogenicity and the like, and can be used in stem cell culture preparations, composite tissue engineering scaffolds, local injection drugs and various dosage forms, and has a wide application prospect in various hard tissue defect repair scenes.

Methods of repairing composite tissue injuries susceptible to adipocyte infiltration, by applying to the injury an electrospun polymer fiber scaffold comprising biocompatible and bioresorbable polymer fibers that are blended with an amount of a GSK-3 inhibitor that is effective to inhibit the infiltration of adipocytes into the scaffold and tissue. Polymer scaffolds blended with an amount of a GSK-3 inhibitor that is effective to inhibit the infiltration of adipocytes into the scaffold and tissue are also disclosed as are kits containing the scaffolds and stem cells for repairing tissue at the injury site.

The invention belongs to the technical field of tissue engineering scaffolds, and relates to a degradable micro-nano fiber composite tissue engineering scaffold and a preparation method thereof.The degradable micro-nano fiber composite tissue engineering scaffold is composed of evenly-distributed micron-sized skeleton fibers and evenly-distributed bioactive nano-sized filling fibers, the diameter of the micron-sized skeleton fibers is 15-25 microns, and the diameter of the bioactive nano-sized filling fibers is 10-20 microns. The diameter difference between the micron-sized skeleton fibers and the bioactive nano-sized filling fibers is more than 50 times; the bioactive nanoscale filling fibers are distributed on the surfaces of the micron-sized skeleton fibers and among different micron-sized skeleton fibers, and the micron-sized skeleton fibers and the bioactive nanoscale filling fibers penetrate through each other to form a three-dimensional graded fiber network structure; during preparation, a spinning solution A and a spinning solution B are prepared respectively, and the degradable micro-nano fiber composite tissue engineering scaffold is prepared by adopting double nozzles to spray the solutions for electrostatic spinning. The degradable micro-nano fiber composite tissue engineering scaffold disclosed by the invention has a three-dimensional interpenetrating hierarchical network structure, long-acting mechanical supporting capability and good cell compatibility.

Provided herein are perfusate compositions, and methods and systems for using such composition to perfuse a vascularized composite tissue (e.g., limb, face, abdominal wall or flap) to preserve such composite tissues (e.g., for auto or allotransplant). In certain embodiments, the perfusate compositions comprise at least one of the following reagents: i) N-acetylcysteine; ii) pantothenate or pantothenic acid at a concentration of at least 15 micromol / liter in said composition; and / or iii) carnitine, as well as further comprising one of the following: i) at least one bicarbonate / CO2-dependent anaplerotic substrate, and at least one bicarbonate / CO2 buffer, or ii) at least one non-bicarbonate / CO2-dependent anaplerotic substrate. In certain embodiments, the perfusate compositions are free, or detectably free, of alpha amino acids.

The application provides a fiber-reinforced composite tissue repair material, characterized in that the repair material comprises a hydrogel matrix layer, a microsphere phase dispersed in the hydrogel matrix layer and a fiber-reinforced layer combined with the hydrogel matrix layer; wherein the hydrogel matrix layer comprises N-acrylated carboxymethyl chitosan and N-acrylated collagen; the microsphere phase is a drug-loaded GelMA microsphere, and the fiber-reinforced layer is an ordered fiber membrane prepared by electrospinning of collagen and alkali-degraded poly(lactic-co-glycolic acid) and has an ordered fiber membrane arranged in a single direction or a layer plate-like arrangement. The fiber-reinforced composite tissue repair material has good transparency, mechanical stability and sustained drug release capacity, can promote tissue cell adhesion, proliferation, directional arrangement and related phenotype expression, inhibit abnormal fibrosis tendency, promote epithelial coverage, and is beneficial to matrix repair and surface reconstruction.

This invention discloses a 3D printing bio-ink, a composite tissue engineering scaffold, and their 3D printing methods and applications. The 3D printing bio-ink comprises component A and component B; component A includes 500-1500 g / L β-tricalcium phosphate, 0.5-5 g / L collagen, 0-10 g / L graphene oxide, and 0.1-5 g / L chitosan and acetic acid; component B includes 100-400 g / L cellulose nanocrystals and water; the volume ratio of components A to B is (0.5-2):1. The 3D printing method for the composite tissue engineering scaffold includes the following steps: loading the 3D printing bio-ink into a 3D printer; designing and importing preset scaffold model parameters; setting the printhead specifications according to the ink properties; correcting the printing offset; determining the printing pressure and speed; setting the printhead temperature and printing platform temperature; forming the preset structure using direct-write printing; pre-drying in an oven followed by supercritical drying to obtain the composite tissue engineering scaffold. The composite tissue engineering scaffold of this invention has excellent biocompatibility and structural stability and can be used in the repair of jawbone defects and other oral tissue regeneration fields.

The invention relates to the field of in-vitro culture and induced differentiation of animal cells, in particular to a culture medium for rapid and synchronous myobiogenesis and adipogenesis serum-free co-induction of fish stem cells and an induction method. According to the method, a serum-free induction culture medium containing specific components is used, nutrition, metabolism and fatty acid delivery conditions required by muscle cell fusion and lipid droplet formation are met in the same induction system at the same time, the process complexity and batch-to-batch fluctuation caused by step-by-step induction are reduced, stable construction of the muscle-lipid composite tissue is achieved, and the method is suitable for large-scale popularization and application. Controllable supply and spectral pattern adjustment of key fatty acid components such as DHA and EPA are supported; synchronous differentiation is started when the cell fusion degree reaches the specified standard, and finally the fish-derived muscle-fat composite tissue with mature muscle fibers and fat droplets at the same time is obtained.

The invention provides a composite tissue bonding patch as well as a preparation method and application thereof. The composite tissue bonding patch comprises a base body and micro-nano fibers formed with the base body in a composite mode. Wherein the micro-nano fibers are continuously or discontinuously dispersed in the base body. The composite tissue bonding patch disclosed by the invention is low in swelling degree, good in mechanical property and lasting in adhesiveness. After the composite tissue bonding patch is in contact with a wet tissue surface, interface liquid existing between the bonding patch and the wet tissue surface can be rapidly absorbed and removed, and a large number of instant molecular bonds can be formed between carboxylic acid and / or amino groups in the composite tissue bonding patch and molecules on the wet tissue surface; and rapid adhesion to a wet surface is realized. Moreover, the swelling degree of the composite tissue bonding patch is low, so that the patch can be prevented from falling off due to deformation caused by excessive water absorption, and the long-time strong adhesiveness can be maintained.

The application relates to a biological 3D printing cartilage-fiber-epithelium integrated tissue engineering trachea and a construction method and application thereof. First, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; epithelium specific biological ink is prepared by wrapping epithelial cells with matrix hydrogel; then, through a step-by-step assembly strategy, a C-shaped cartilage ring and a fiber ring are alternately printed and a ring-shaped tubular structure is formed by using a double-needle extrusion type biological 3D printing technology, and then a layer of epithelium specific biological ink is attached in the lumen to construct a tubular structure of cartilage-fiber-epithelium multi-tissue composite; it is verified that after being implanted subcutaneously in a naked mouse for 8 weeks, a relatively mature cartilage-vascularized fiber-epithelium tissue composite tissue engineering trachea can be regenerated, and a clinically transformable strategy is provided for the regeneration treatment of complex tracheal injury.