Preparation method of an in situ pore-forming self-setting calcium phosphate composite tissue engineering scaffold

A composite tissue, calcium phosphate technology, applied in the field of biomedical engineering, can solve the problems of decreased scaffold strength, low strength of calcium phosphate materials, poor mechanical properties, etc., and achieve good strength, good mechanical properties and cell affinity, strength. improved effect

Inactive Publication Date: 2007-08-01
SOUTH CHINA UNIV OF TECH
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  • Summary
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  • Application Information

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Problems solved by technology

However, in the course of many years of research and application, the problems of low strength and poor mechanical properties of calcium phosphate materials have not been well resolved. The mechanical properties are only between cancellous bone and cortical bone, and the brittleness is high. And its poor load-bearing abil

Method used

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  • Preparation method of an in situ pore-forming self-setting calcium phosphate composite tissue engineering scaffold

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Embodiment 1

[0027] 1. Dissolve gelatin in deionized water, stir with a thermostatic magnetic stirrer at 60°C for 2 hours to obtain a gelatin aqueous solution with a gelatin mass percentage of 30%. Leave the prepared solution to stand for 1 hour, filter to remove insolubles, and put it in a container In standby.

[0028] 2. Select α-tricalcium phosphate (α-TCP) + calcium hydrogen phosphate dihydrate (DCPD) + calcium carbonate (CaCO) with a total porosity of about 70% and a pore size of 100~400μm. 3 ) + Hydroxyapatite (HA) + Magnesium phosphate (Mg 3 (PO 4 ) 2 )+Sodium hydrogen phosphite (NaHSO 3 ) System bone cement porous scaffold material, put the porous scaffold into a microwave drying oven, dry at 37°C for 0.5 hours, take it out, immerse it in the gelatin aqueous solution, put it in a vacuum dryer, vacuum for 4 hours, make the gelatin aqueous solution under pressure and It is poured into the pores of the porous stent under the action of capillary force.

[0029] 3. Take out the stent mater...

Embodiment 2

[0031] 1. Prepare 0.05% chitosan solution and 4% gelatin solution with 1% acetic acid, stand for 48 hours, filter separately, and then mix the two solutions according to the volume ratio of 1:1 , And obtain chitosan / gelatin solutions with chitosan and gelatin mass percentages of 0.025% and 2%, respectively, for use.

[0032] 2. Use β-tricalcium phosphate (β-TCP) + calcium hydrogen phosphate dihydrate (DCPD) system bone cement porous scaffold material with a total porosity of about 40% and a pore size of 50-300μm, and put the porous scaffold into ordinary electric heating In a drying box, dry for 60 hours at 30°C, take it out, immerse it in the chitosan / gelatin aqueous solution prepared in step 1, put it in a vacuum dryer, vacuum for 0.5 hours, make the chitosan / gelatin aqueous solution under pressure and capillary force Under the action, it is poured into the pores of the porous scaffold.

[0033] 3. Take out the scaffold material filled with chitosan / gelatin blend in the pores, d...

Embodiment 3

[0035] 1. Prepare a silk fibroin solution with a mass percentage concentration of 1% and a sodium alginate solution with a mass percentage concentration of 6% with distilled water, stand for 24 hours, filter separately, and then mix the two solutions in a volume ratio of 1:1. Glutaraldehyde, which accounts for 1% of the total protein content of the solution, is added as a cross-linking agent to obtain a silk fibroin / sodium alginate mixed solution with a mass content of 0.5% and 3% of silk fibroin and sodium alginate, respectively, for use.

[0036] 2. Use tetracalcium phosphate (TTCP) + anhydrous calcium hydrogen phosphate (DCPA) + calcium carbonate (CaCO) with a total porosity of about 90% and a pore size of 50~400μm 3 ) System bone cement porous scaffold material, put the porous scaffold into a drying box, dry at 60°C for 30 hours, take it out, immerse it in the silk fibroin / sodium alginate aqueous solution prepared in step 1, put it in a vacuum bottle, and vacuum for 4 hours , ...

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Abstract

The invention discloses a making method of composite tissue engineering rack of original poring self-solidifying calcium phosphate, which comprises the following steps: placing porous rack of calcium phosphate cement into drier to dry; immersing porous rack into macromolecular solution; extracting into vacuum for 0.5-6h; injecting macromolecular material into the pore of porous rack of calcium phosphate cement; drying the surface of porous rack through filter paper; pre-freezing under -60- -4 deg.c for 1-48h; drying the frozen material to do multiple injections until the pore is filled with cement; obtaining the product.

Description

Technical field [0001] The invention belongs to bone tissue engineering material technology in the field of biomedical engineering, and particularly relates to a method for preparing an in-situ pore-forming self-solidifying calcium phosphate composite tissue engineering scaffold. Background technique [0002] Bone tissue damage caused by injuries and illnesses seriously affects people's health and quality of life. The current clinical treatment methods mainly include: autologous bone transplantation, allogeneic bone transplantation and artificial material repair. Although autologous bone is an ideal bone graft material, the source of donor bone is limited. The second operation will cause pain to the patient and cause new damage to the donor. The donor area may also have morphological and functional disorders; although the source of allogeneic bone It is abundant, can be stored in advance, and is convenient to use, but it has immune rejection reaction, and due to omissions in the ...

Claims

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Application Information

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IPC IPC(8): A61L27/42
Inventor 叶建东董浩王迎军
Owner SOUTH CHINA UNIV OF TECH
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