199results about How to "Reduce biological activity" patented technology

The present invention relates in part to methods for suppressing the innate immune response of a cell to transfection with an exogenous nucleic acid, to methods for increasing expression of a protein encoded by an exogenous nucleic acid by repeated delivery of the exogenous nucleic acid to a cell, and to methods of changing the phenotype of a cell by differentiating, transdifferentiating or dedifferentiating cells by repeatedly delivering one or more nucleic acids that encode defined proteins. A method is provided for extended transient transfection by repeated delivery of an in vitro-transcribed RNA (“ivT-RNA”) to a cell to achieve a high and sustained level of expression of a protein encoded by an ivT-RNA transcripts.

Compositions, systems and related methods for delivering a supercharged protein or a complex of a supercharged protein and therapeutic agent (e g, nucleic acid, peptide, small molecule) to cells are disclosed. Superpositively charged proteins may be associated with nucleic acids (which typically have a net negative charge) via electrostatic interactions. The systems and methods may involve altering the primary sequence of a protein in order to “supercharge” the protein (e g, to generate a superpositively-charged protein). The compositions may be used to treat proliferative diseases, infectious diseases, cardiovascular diseases, inborn errors in metabolism, genetic diseases, etc.

Disclosed are novel organopolysiloxane compositions that provide good deposition to the surface of fabric and hair. When incorporated in fabric treatment compositions, they provide anti-wrinkle benefit, softness, and anti-static benefit. When incorporated in hair conditioner compositions, they provide conditioning benefit and anti-static benefit.

The invention provides a soil improvement type straw compound organic fertilizer and a preparation method of the soil improvement type straw compound organic fertilizer. The soil improvement type straw compound organic fertilizer is prepared from the following raw materials in parts by weight: 30 to 35 parts of rice straw, 25 to 30 parts of animal excrement, 20 to 25 parts of biological charcoal, 10 to 15 parts of soybean meal, 5 to 10 parts of cassava residue, 5 to 10 parts of diatom ooze, 2 to 2.5 parts of plant ash, 2 to 2.5 parts of turfy soil, 2 to 3 parts of zeolite powder, 3 to 4 parts of a microorganism bacterium agent, 2 to 3 parts of sodium benzoate, 2 to 3 parts of ammonium monohydrogen phosphate, 1 to 2 parts of calcium chloride, 1 to 2 parts of amino acid, 1 to 2 parts of starch, 1 to 2 parts of molasses powder and 1 to 2 parts of humic acid. The soil improvement type straw compound organic fertilizer takes plant wastes as raw materials; the quality of soil can be easily improved and the production cost can also be reduced, so that the physicochemical properties of the soil are improved, and the structure of the soil is comprehensively improved, and furthermore, the soil quality has the effects of softness, air permeability, water retention and fertilizer conservation; the aim of producing an environment-friendly high-quality organic fertilizer through preferably selecting heavy metal capable of being passivated, the biological charcoal capable of improving the fertilizer efficiency of the organic fertilizer, and conversion products of organic wastes including the biological humic acid and the like as composting base materials, and optimizing a composting process.

The invention provides a broad spectrum heavy metal activity deactivator preparation method. The present invention relates to a soil conditioning agent preparation method, which comprises the following steps: (A) taking humic acid-rich peat, digest compost or a forest soil humus layer, and carrying out screening to provide a spare use; (B) adding the humic acid-rich material to HCl or HNO3 to soak, and stirring 2-3 times; (C) filtering the soaked material with a bamboo drying pad, washing 2-3 times with clear water, and carrying out air drying to a moisture content of less than 5%; (D) sequentially adding ZnSO4.7H2O, sodium silicate and sodium selenite to the material obtained from the step (C), carrying out aging on the mixed material for 2-3 days and uniformly mixing; and (E) adding limestone powder to the material obtained from the step (D), and completely and uniformly mixing to prepare the broad spectrum heavy metal deactivator. With the combination inhibitor prepared by the preparation method, activities of soil heavy metals can be significantly inactivated, the heavy metals can be inhibited from entering the food chain, and risks on human health by the heavy metals can be reduced.

The invention discloses a method for preparing a false tooth support through a 3D printing laser stereo-lithography technology. According to the method for preparing the false tooth support through the 3D printing laser stereo-lithography technology, the individual false tooth support most suitable for a patient is printed through the 3D printing laser stereo-lithography technology; the comfort level and the service life of the false tooth support are ensured by means of a finite element technology; the operation difficulty of a physician is lowered; the manufacturing cost is reduced; the manufacturing period is shortened. The surface of the false tooth support is processed through an anodizing and/or electrochemical deposition method so that the surface of the false tooth support can have more excellent synosteosis features and biology activity, osteoblast growth and surface calcification are facilitated, and the satisfaction of the patient is improved. The method is suitable for the field of medical devices.

The invention relates to the field of soil fertilizer and provides a biological organic repairing agent for soil pollution and its application and preparation method. The biological organic repairing agent of the invention is composed of, by weight, 1.5-3 parts of eymogenic bacterium, 1-1.5 parts of wheat bran, 1.5-2 parts of molasses meal, 45-50 parts of ammonium humate, 45-50 parts of hydrolase dregs, 150-200 parts of soybean meal, 100-150 parts of humic acid powder, 80-100 parts of zeolite powder, 25-40 parts of a calcium magnesium phosphate fertilizer, 150-200 parts of cattle and sheep manure, 150-204 parts of plant straw, 25-40 parts of potassium humate, 1.5-3 parts of bacillus mucilaginosus, 1-1.5 parts of Gliocladium roseum, 1.5-2 parts of Streptomycesvinaceus-drappus, 1-1.5 parts of trichoderma spp, 1-1.5 parts of Paecilomyces lilacinus, 1-1.5 parts of bacillus subtilis and 1-1.5 parts of micrococcus. According to the invention, soil pollution control, improvement and nutrition are integrated.

Disclosed is a method and test kit to diagnose tumorigenicity or the presence of tumor cells in a patient by detecting the level of FBLN-3 expression or biological activity in the patient. Also disclosed are methods to identify regulators of tumor cell growth, motility and/or invasion, by identifying regulators of FBLN-3 expression or activity. Methods to identify anti-angiogenic and pro-angiogenic agents are also described, wherein such factors regulate the expression and/or activity of FBLN-3. Finally, the present invention relates to therapeutic methods and reagents for the inhibition of tumor growth and development and/or for the inhibition or promotion of angiogenesis, using FBLN-3, homologues and analogs thereof, and agents that modulate the expression and/or activity of FBLN-3.

The invention discloses soil improvement organic biological fertilizer and a preparation method thereof. The soil improvement organic biological fertilizer comprises the following raw materials in parts by weight: 30 to 35 parts of rice straws, 25 to 30 parts of animal dung, 20 to 25 parts of charcoal, 10 to 15 parts of bean pulp, 5 to 10 parts of diatom ooze, 2 to 2.5 parts of soddy soil, 2 to 3 parts of zeolite powder, 3 to 4 parts of a microbial agent, 2 to 3 parts of sodium benzoate, 2 to 3 parts of monoammonium phosphate, 2 to 3 parts of mugwort, 2 to 2.5 parts of licorice, 1 to 2 parts of calcium chloride, 1 to 2 parts of amino acid, 1 to 2 parts of starch, 1 to 2 parts of molasses powder, and 1 to 2 parts of humic acid. The soil improvement organic biological fertilizer takes plant waste as the raw material, not only is beneficial to improve the soil quality, but also can reduce the production cost, thereby improving the physicochemical property of soil, comprehensively improving the soil structure, and enabling the soil quality to achieve the effects of softness, permeability, moisture holding and fertilizer maintenance, and optimizes the composting process by preferentially selecting organic waste converted products capable of passivating heavy metal and enhancing the fertilizer efficiency of organic fertilizer, such as charcoal, biological humic acid of and the like, as compost base materials, so as to achieve the purpose of producing environmental high-quality organic fertilizer.

The present invention relates to non-activated Wnt inhibition polypeptides (WIPs) containing: (a) a protein transduction domain (PTD) which enables said WIPs to permeate a cell membrane without the aid of a cell membrane receptor; and (b) a Wnt antagonist domain which is inactive by itself, but is activated in mammalian cells and then secreted out of the cells to function to inhibit Wnt signal transduction. Also, the invention relates to a method for preparing said non-activated WIPs, and a pharmaceutical composition containing said WIPs as active ingredients. Said non-activated WIPs can be produced in large quantities through the culture of bacteria such as E coli., and are biochemically inactive before being administered into the human body, and thus the production cost thereof is only one several tenths of that of previously known active proteins (sFRPs, DKKs, etc.) having uses similar thereto, and the isolation/purification and handling/administration processes thereof are significantly simple and convenient. When said non-activated WIPs are administered in vivo, they will have the effects of inhibiting the invasive growth and metastasis of cancer cells and treating immune diseases, such as rheumatoid arthritis by pharmacological mechanisms different from those of the previously known sFRPs or DKKs.

This invention is preparation procedure of the yolk antibody of high biological activity, belonging to the field of bioproduct and industrialized isolation technique. In this invention, we adopt the natural macromolecule gel high -extractive technique and hyperfiltration to extract the yolk-antibody of high biological activity from yolk at an industrialized level, annual output is upto 10 ton. the yolk-antibody content is about 30%-50%, meanwhile we could obtain some by-products like albumen powder, yolk powder, egg oil, vitellin and so on. In the invention high technology like bioimmunology and membrane separation is adopted. The invention is high-tech, high-usage, highly-automated and typical of green food and environmental-friendly.

The invention discloses a method for preparing lycium ruthenicum pigment microcapsules by using a piercing method. The method comprises the following steps of using lycium ruthenicum fresh fruits or lycium ruthenicum dried fruits to serve as raw materials, crushing, conducting acidity ethanol digestion, extracting by using a microwave extractor, extracting residues for 2-3 times by using the acidity ethanol after filtering, combining an extracting solution, and conducting decompression concentration to obtain a pigment concentrated solution; and conducting adsorption, elution, desorption and decompression concentration on the pigment concentrated solution through macroporous resin, adding an embedding medium in proportion, conducting solidification, filtering, laminating, filtering and return soaking, drying under ordinary pressure at the temperature of 40-50 DEG C to obtain the lycium ruthenicum pigment microcapsules. An embedding ratio of the lycium ruthenicum pigment microcapsules prepared by the process is 70%-80%, lycium ruthenicum pigments have a slow release function after microencapsulation, more than 70% pigments can be released within 24 hours, the shape is kept to be complete, stability of the lycium ruthenicum pigment microcapsules in an 75% of relative humidity environment is obviously improved, and the weight increase of the microcapsules caused by moisture absorption is lower than 10%.

The invention provides deimmunized mutant proteins having reduced immunogenicity while exhibiting substantially the same or greater biological activity as the proteins of interst from which they are derived, as exemplified by mutant L-asparaginase that comprises amino acid substitutions compared to wild type L-asparaginase. The invention further provides methods for screening mutant deimmunized proteins that have substantially the same or greater biological activity as a protein of interest, and methods for reducing immunogenicity, without substantially reducing biological activity, of a protein of interest.

The invention's compositions and methods are useful in, for example, therapeutic applications by minimizing adverse immune responses by the host mammalian subjects to the protein of interest. Thus, the invention further provides methods for treating disease comprising administering to a subject a therapeutically effective amount of a pharmaceutical composition comprising at least one of the mutant deimmunized proteins produced by the invention's methods.

The invention discloses a fluorine-containing waterborne polyurethane material with waterproof, oil-proof and anti-fouling properties. The preparation method comprises the following steps: firstly, synthesizing a dihydroxy fluorocarbon chain monomer through sulfydryl-vinyl Michael addition reaction; carrying out addition polymerization reaction with a diisocyanate-terminated polyurethane prepolymer to introduce the prepolymer into polyurethane, and respectively carrying out chain extension reaction and neutralization reaction to obtain the fluorine-containing waterborne polyurethane material with the waterproof, oil-proof and anti-fouling properties. The fluorine-containing waterborne polyurethane material has a wide application prospect in the fields of biomedicine, leather finishing agents and the like.

The invention relates to a rice-straw antibacterial soil amendment. The rice-straw antibacterial soil amendment is prepared from the following raw materials in parts by weight: 30-35 parts of rice straws, 20-30 parts of biochar, 10-15 parts of bean meal, 5-10 parts of cassava residue, 10-15 parts of chicken manure, 5-10 parts of diatom ooze, 2-3 parts of artemisia argyi, 2-2.5 parts of glycyrrhiza uralensis, 2-2.5 parts of pine needles, 2-3 parts of tea tree oil, 2-2.5 parts of plant ash, 2-3 parts of zeolite powder, 2-3 parts of microbial functional bacterium agent, 2-3 parts of sodium benzoate, 1-2 parts of amino acid, 1-2 parts of starch and 1-2 parts of molasses powder. According to the rice-straw antibacterial soil amendment, plant wastes are adopted as the raw materials, so that the improvement on quality of soil is facilitated, the production cost can be reduced; the physicochemical properties of the soil are improved, the structure of the soil is comprehensively improved, and the texture of the soil can achieve soft, air-permeable, water-retention and fertilizer retention effects; and the rice-straw antibacterial soil amendment plays roles in sterilizing, weeding, increasing the utilization ratio of chemical fertilizers, promoting the root system development of crops, increasing crop yield and improving product quality.

The present invention relates to an inner-alpha inhibitor protein (I alpha Ip). The invention also relates to a method for purifying the I alpha Ip composition and the usage for treating the human diseases such as pyaemia, septicemic shock, atrophic arthritis, cancer and infectious disease.

The invention discloses a preparation method of high-purity lycium polysaccharides. The method includes the following steps that lycium barbarums or lycium-barbarum waste residues are crushed, sievedand then subjected to subcritical degreasing, high-speed shearing, low-temperature wall breaking, water extraction, high-speed centrifugation, microfiltration-membrane separation, microfiltration-membrane concentration, inorganic/organic two-phase extraction, impurity removal with macroporous adsorption resin, ethanol precipitation and freeze-drying to obtain the high-purity lycium polysaccharides. It is tested that the active ingredient, namely, lycium barbarum polysaccharide, in the extract is not less than 80%. The lycium polysaccharides are separated by using a 30 kD microfiltration membrane, only the lycium polysaccharides with the molecular weight less than 30 kD are obtained, the lycium polysaccharides with the molecular weight not less than 30 kD are removed, as the lycium polysaccharides with the molecular weight not less than 30 kD are metabolized slowly in vivo, massive accumulation is resulted, 'internal heat' can be induced, and the biological activity of the lycium polysaccharides with the molecular weight not less than 30 kD is low. The four purification technologies are adopted, such as nanofiltration-membrane concentration, two-aqueous-phase extraction, impurity removal with macroporous adsorption resin and ethanol precipitation, and the purity and quality of the obtained lycium polysaccharides are ensured.

A non-fat creamer composition that contains from about 10% to about 90%, of a microparticulated protein component and from about 10% to about 90%, of a insoluble microcrystalline cellulose component. In the hydrated state the microparticulated protein component particles have a mean diameter particle size distribution ranging from about 0.1 microns to about 10.0 microns, and preferably less than about 5 percent of the total number of particles exceed about 10.0 microns in diameter. Further, the creamer composition contains less than about 2% of fat and oil. These non-fat creamer compositions can be powdered and liquid, dairy and non-dairy, and can be prepared in both concentrated and ready-to-use forms. The powdered creamer compositions are well suited for use in instant and/or dry food and beverage compositions that require the addition of water or other suitable fluids prior to use.

The invention discloses an in-situ combined remediation technology for contaminated soil. The in-situ combined remediation technology adopts a method for combined use of an in-situ leaching technology and an in-situ stabilization solidification technology, and comprises the following steps: feeding leaching liquid into an injection well, extracting the pollutant-containing leaching liquid through an extraction well, and treating the pollutant-containing leaching liquid in wastewater treatment equipment; after leaching, injecting a stabilization agent into a polluted region through the injection well adopted in an in-situ leaching process, and controlling the flow direction of the stabilization agent by virtue of the extraction well to realize recycling; after stabilizing, injecting a solidification agent by virtue of the injection well, isolating site pollutants from an environment, and filling soil gaps. According to the in-situ combined remediation technology, the advantages of in-situ leaching and in-situ stabilization/solidification are combined and a treatment effect is good.

The invention discloses a method for preparing a medicine with liver target taxis for slow-releasing the nanometer microcapsule. The method comprises the following steps: proceeding with enzymatic reaction of liver target taxis gene with sugar monomer of cerebrose residue and ethylated carboxylate; copolymerizing vinyl ester with glycosyl and unsaturated cationic or anion; getting liver target taxis polyelectrolyte; getting the medicinal microcapsule with liver target taxis by multilayer packaging polyelectrolyte on the surface of medicine by electrostatic action, wherein the deactivation speed of microcapsule medicine can be controlled. The polymerization method is simple, high effective and non-toxicity. The coating method has the high efficient, the simple operation and the mild technology, which can cycle several times. The preparing medicine can save for a long time, which can release step by step, can accumulate in the liver, improves the medicinal effect of disease portion, reduces the toxic effect of the other healthy organ, and has the good application prospect.

The invention relates to a production method of a bio-transformed selenium preparation and a production method of a selenium-enriched agricultural product. The production method of the bio-transformed selenium preparation comprises the following steps: preparing a culture medium in accordance with strain medium components, keeping the culture medium in a transparent glass bottle, sterilizing by virtue of a medical high-pressure sterilizing pot at 121 DEG C and keeping for 20min, taking out the culture medium, and inoculating a strain which is 10-20% of the amount of the culture medium when the culture medium is cooled to room temperature, wherein the inoculating process needs to be operated on a super-clean bench in a sterile environment, the temperature of a cultivating process is 25-35 DEG C, illumination intensity is 1500-3500Lux, the strain is cultivated for 3-6 days, and a grown culture solution is in dark red or brown and contains 0.2-0.8 to the power of 8 billion/mL living effective bacteria. The method disclosed by the invention has the beneficial effects that 1. inorganic selenium, which is low in bioactivity, is converted into organic selenium, which is high in bioactivity, through microorganism transformation, and the organic selenium is low in toxicity, safe to use and high in absorption and utilization rates in animals and plants; 2. the bio-transformed selenium preparation is broad in application scope and strong in applicability, and the preparation is free from regional limitation when used; and 3. the bio-transformed selenium preparation is convenient to use and simple to operate.

The invention discloses a preparation formula of a gene therapy medicament taking a recombinant adeno-associated virus (rAAV) as a vector. A preparation in the formula comprises a rAAV gene therapy medicament as well as excipient components such as a protective human serum albumin for preventing container adsorption, a buffer pair of citric acid and sodium citrate for maintaining the pH value of a solution in a neutral range and a surfactant polyether F-68 for preventing polymerization among rAAV gene vectors. The preparation does not cause the rapid descending of the biological activity under a room temperature condition, and does not require cryopreservation.