Production method of bio-transformed selenium preparation and production method of selenium-enriched agricultural product
A biotransformation and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low absorption rate of animals and plants, low effective utilization rate of selenium, and high toxicity of inorganic selenium, reaching the application range Wide range, high absorption and utilization rate of animals and plants, simple operation
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Embodiment 1
[0047] Example 1: (Production of biotransformed selenium preparation 1)
[0048] Strain culture medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate (MgSO 4 ·7H 2 O): 0.5g; calcium chloride CaCl 2 :0.3g; Distilled water: 1000mL; pH value: 6.5~7.5.
[0049] Fermentation medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; sodium selenite Na 2 SeO 3 ·5H 2 O: 0.8 g; tap water: 1000 mL; pH value: 6.5 to 7.5.
[0050] Strain preparation: Prepare the culture medium according to the above-mentioned culture medium composition, put it into a glass bottle, sterilize it in a medical autoclave at 121°C for 20 minutes, take it out to cool to room temperature, and pick up 10% of the culture medium. Into the strain. Cultivation process temperature: 35°C, light intensity 2500 Lux, culture for 5 days, the color of the culture medium that grows is dark red, and the effective number of viable bacteria is 3.0×1...
Embodiment 2
[0052] Example 2: (Production of biotransformed selenium preparation 2)
[0053] Strain culture medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; Distilled water: 1000mL; pH value: 6.5~7.5.
[0054] Fermentation medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; selenium dioxide SeO 2 :0.5g; Tap water: 1000mL; pH value: 6.5~7.5.
[0055] Strain preparation: prepare the culture medium according to the composition of the culture medium, put it into a glass bottle, sterilize it in a medical autoclave at 121°C for 20 minutes, and when it is taken out to be cooled to room temperature, connect it according to 20% of the culture medium. Strains. Cultivation process temperature: 28°C, light intensity 1500 Lux, culture for 6 days, the color of the culture solution after growth is brown, and the effective number of viable bacteria is 5.0×10 8 B...
Embodiment 3
[0057] Example 3: (Production of Se-enriched Vegetables)
[0058] During the planting of Chinese cabbage, the 120-fold dilution of the biotransformed selenium preparation was sprayed twice at intervals, and the selenium content in the Chinese cabbage was detected after harvest, and the content reached 0.08 mg / kg. The measured value is higher than the requirement of selenium content in selenium-rich foods for fruits and vegetables (without juice) ≥0.01mg / kg; lower than the "National Health Standards" for selenium in foods, vegetables ≤0.1mg / kg After using the biotransformed selenium preparation, the yield of Chinese cabbage was increased by 8%, and the quality of Chinese cabbage was also significantly improved. Compared with the control, the soluble sugar increased by 23.6% and the vitamin C increased by 17.1%.
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