Production method of bio-transformed selenium preparation and production method of selenium-enriched agricultural product

A biotransformation and production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low absorption rate of animals and plants, low effective utilization rate of selenium, and high toxicity of inorganic selenium, reaching the application range Wide range, high absorption and utilization rate of animals and plants, simple operation

Active Publication Date: 2016-04-27
广州天壤生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In the production of selenium-enriched products on the market, most of the selenium used is inorganic. Inorganic selenium is highly toxic, unsafe, has a single effect, low absorption rate of animals and plants, low effective utilization of selenium, and poor selenium enrichment effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: (Production of biotransformed selenium preparation 1)

[0048] Strain culture medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate (MgSO 4 ·7H 2 O): 0.5g; calcium chloride CaCl 2 :0.3g; Distilled water: 1000mL; pH value: 6.5~7.5.

[0049] Fermentation medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; sodium selenite Na 2 SeO 3 ·5H 2 O: 0.8 g; tap water: 1000 mL; pH value: 6.5 to 7.5.

[0050] Strain preparation: Prepare the culture medium according to the above-mentioned culture medium composition, put it into a glass bottle, sterilize it in a medical autoclave at 121°C for 20 minutes, take it out to cool to room temperature, and pick up 10% of the culture medium. Into the strain. Cultivation process temperature: 35°C, light intensity 2500 Lux, culture for 5 days, the color of the culture medium that grows is dark red, and the effective number of viable bacteria is 3.0×1...

Embodiment 2

[0052] Example 2: (Production of biotransformed selenium preparation 2)

[0053] Strain culture medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; Distilled water: 1000mL; pH value: 6.5~7.5.

[0054] Fermentation medium composition: yeast powder: 3.0g; peptone: 3.0g; magnesium sulfate MgSO 4 ·7H 2 O: 0.5g; calcium chloride CaCl 2 :0.3g; selenium dioxide SeO 2 :0.5g; Tap water: 1000mL; pH value: 6.5~7.5.

[0055] Strain preparation: prepare the culture medium according to the composition of the culture medium, put it into a glass bottle, sterilize it in a medical autoclave at 121°C for 20 minutes, and when it is taken out to be cooled to room temperature, connect it according to 20% of the culture medium. Strains. Cultivation process temperature: 28°C, light intensity 1500 Lux, culture for 6 days, the color of the culture solution after growth is brown, and the effective number of viable bacteria is 5.0×10 8 B...

Embodiment 3

[0057] Example 3: (Production of Se-enriched Vegetables)

[0058] During the planting of Chinese cabbage, the 120-fold dilution of the biotransformed selenium preparation was sprayed twice at intervals, and the selenium content in the Chinese cabbage was detected after harvest, and the content reached 0.08 mg / kg. The measured value is higher than the requirement of selenium content in selenium-rich foods for fruits and vegetables (without juice) ≥0.01mg / kg; lower than the "National Health Standards" for selenium in foods, vegetables ≤0.1mg / kg After using the biotransformed selenium preparation, the yield of Chinese cabbage was increased by 8%, and the quality of Chinese cabbage was also significantly improved. Compared with the control, the soluble sugar increased by 23.6% and the vitamin C increased by 17.1%.

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Abstract

The invention relates to a production method of a bio-transformed selenium preparation and a production method of a selenium-enriched agricultural product. The production method of the bio-transformed selenium preparation comprises the following steps: preparing a culture medium in accordance with strain medium components, keeping the culture medium in a transparent glass bottle, sterilizing by virtue of a medical high-pressure sterilizing pot at 121 DEG C and keeping for 20min, taking out the culture medium, and inoculating a strain which is 10-20% of the amount of the culture medium when the culture medium is cooled to room temperature, wherein the inoculating process needs to be operated on a super-clean bench in a sterile environment, the temperature of a cultivating process is 25-35 DEG C, illumination intensity is 1500-3500Lux, the strain is cultivated for 3-6 days, and a grown culture solution is in dark red or brown and contains 0.2-0.8 to the power of 8 billion/mL living effective bacteria. The method disclosed by the invention has the beneficial effects that 1. inorganic selenium, which is low in bioactivity, is converted into organic selenium, which is high in bioactivity, through microorganism transformation, and the organic selenium is low in toxicity, safe to use and high in absorption and utilization rates in animals and plants; 2. the bio-transformed selenium preparation is broad in application scope and strong in applicability, and the preparation is free from regional limitation when used; and 3. the bio-transformed selenium preparation is convenient to use and simple to operate.

Description

[0001] This strain has been deposited in the Chinese Type Culture Collection of Wuhan University, China on September 27, 2014. The culture name is RhodopseudomonaspalustrisTR1 (RhodopseudomonaspalustrisTR1), and the preservation number is CCTCCNO: M2014447. Technical field [0002] The invention relates to a biological transformation and application in the technical field of agricultural products, in particular to a production method of a biological transformation selenium preparation and a production method of selenium-enriched agricultural products. Background technique [0003] Selenium is a trace element that cannot be synthesized in the human body. The nutritional research of selenium has become one of the hot spots in the world, especially in Europe and the United States. The more people know about selenium, the more they realize how important it is to health. [0004] In 1973, the United Nations Health Organization announced that selenium is an essential trace element for t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01C21/00A23K10/18A23K50/75A23K50/80C12R1/01
CPCY02A40/818
Inventor 李琦吴坚伟曹养宪
Owner 广州天壤生物科技有限公司
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