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The application belongs to the technical field of tunnel soil body reinforcement, and particularly discloses a device and method for reinforcing soil body of a tunnel starting well portal by injecting microbial slurry, which comprises a storage component, a transmission component and an injection component, the storage component comprises two constant-temperature boxes, and the two constant-temperature boxes are respectively used for placing bacterial liquid and cementing liquid; the transmission component comprises a multi-connection micro high-pressure pump, and two independent liquid channels are arranged in the pump; the injection component comprises a grouting guide pipe and a rotating grouting head, the grouting guide pipe comprises two hollow semi-cylindrical pipes, and the longitudinal straight faces of the two hollow semi-cylindrical pipes are attached together to form a cylinder with two independent longitudinal cavities; the two independent longitudinal cavities are respectively connected with the two constant-temperature boxes through different liquid channels; a plurality of spray holes are longitudinally and uniformly arranged on the arc faces of the hollow semi-cylindrical pipes; and the rotating grouting head is used for driving the grouting guide pipe to rotate. The application can reduce the internal loose problem of the soil body reinforced by the microorganism, and improve the reinforcement effect of the soil body of the tunnel starting well portal.