Biological enzyme-catalyzed wall-breaking microalgae grease extraction method

A microalgae oil and bio-enzyme technology, applied in the field of microbial engineering, can solve the problems of low compound enzyme content, difficulty in achieving beneficial effects, and long oil extraction cycle, so as to reduce the amount of enzyme used, reduce extraction costs, shorten The effect of the extraction cycle

Inactive Publication Date: 2019-04-12
HEXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method is easy to operate and easy to operate, it is aimed at the efficient oil extraction of oleaginous microalgae, especially photoautotrophic oleaginous microalgae, which reduces the energy input of the microalgae oil extraction process and improves the energy efficiency ratio of microalgae energy. , thereby greatly reducing the comprehensive production cost of microalgae biomass energy; however, the entire oil extraction cycle is too long, and the content of the compound enzyme added cannot be too low, otherwise it will be difficult to achieve the beneficial effect described above, which virtually increases production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0027] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 13 days under the conditions of a culture temperature of 25°C and a light intensity of 5500lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0028] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella, wherein the enzyme activity of the protease is 35000U / g, stir the reaction system and heat to 45°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0029] 3) rapidly cooling the boiled reaction solution to 50° C., and adding a compound enzym...

Embodiment 2

[0032] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0033] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 15 days at a culture temperature of 25°C and a light intensity of 5000lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0034] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella quality, wherein the enzyme activity of the protease is 40000U / g, stir the reaction system and heat to 50°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0035] 3) Rapidly cooling the boiled reaction solution to 55° C., and adding a compound enzyme composed of...

Embodiment 3

[0038] A method for bio-enzyme-catalyzed wall-breaking to extract oil from microalgae, comprising the following steps:

[0039] 1) Take an appropriate amount of chlorella and place it in a solid medium, culture it for 15 days at a culture temperature of 25°C and a light intensity of 5000lx, then scrape the microalgae from the surface of the solid medium and put it into warm water, and heat it at 1500rpm High-purity chlorella was obtained by centrifuging at a high speed for 15 minutes;

[0040] 2) Put 500g of high-purity chlorella into purified water, then add a phosphate buffer with a volume of 5% of the volume of pure water and a protease with a weight of 0.5% of the high-purity chlorella, wherein the enzyme activity of the protease is 35000U / g, stir the reaction system and heat to 48°C, continue to heat up to 100°C and boil for 10min after reacting for 0.5h;

[0041] 3) Rapidly cooling the boiled reaction solution to 55° C., and adding 0.8% by weight of high-purity chlorel...

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PUM

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Abstract

The invention discloses a biological enzyme-catalyzed wall-breaking microalgae grease extraction method. The method comprises the following four steps: microalgae culture centrifugal screening, protease treatment and boiling, ultrasonic treatment after combined enzyme treatment and distillation extraction. Through change of the addition order of enzymes and control on the temperature of a solution, the activity of the enzymes is maximized, the use amount of the enzymes is reduced, and decomposition of whole microalgae cells is accelerated finally through short-term ultrasonic treatment; meanwhile, the decomposition products is isolated from grease on microalgae, and subsequent grease extraction is facilitated; and not only can the extraction rate of internal grease of the microalgae be improved, but also the use amount of the enzymes is reduced, the extraction cycle is shortened, and the extraction cost is reduced greatly.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a method for extracting oil from microalgae by catalyzing bio-enzyme breaking walls. Background technique [0002] Algae biofuels are an important part of the global renewable resource system. As the main form of biofuel, the oil in microalgae is biodiesel prepared by using the oil in microalgae to react under catalyst conditions. Compared with traditional biofuels, algae biofuel has more advantages. Among them, some microalgae are most useful because their stem cells are rich in microalgae oil of more than 70%, which is the best raw material for subcritical biotechnology to synthesize biodiesel, and is an ideal renewable energy source. [0003] At present, the techniques for extracting the internal oil of microalgae mainly include traditional physical methods such as mechanical crushing, ultrasonic wave, colloid mill, and biological enzymatic hydrolysis methods. T...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/10C11B3/12
CPCC11B1/02C11B1/025C11B1/106C11B3/12
Inventor 王丽娟罗光宏陈天仁杨宋琪
Owner HEXI UNIV
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