312 results about "Enzyme catalyzed" patented technology

The invention relates to the technical field of biological catalysis, and discloses an L379A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: leucine of the 379th amino acid sequence is mutated into alanine; the enzyme can be applied to RA in-vitro conversion preparation of RI with higher utilization value, the conversion rate is higher than 50%, and the enzyme activity is remarkably improved by 7 times compared with the original enzyme catalytic conversion enzyme activity. The UGT76G1 mutant disclosed by the invention has the advantages that (1) the blank is filled, and a special enzyme catalyst capable of realizing efficient in-vitro synthesis of rebaudioside I (RI) is provided for the first time; 2) efficiency jump: the catalytic conversion rate is greatly increased from original about 7% to more than 50% (increase gt; and 3) stability and reliability: in the cross-scale reaction of 10mL to 5L, the catalyst has stable catalytic performance, shows excellent industrial application potential, and is suitable for popularization and application.

The invention belongs to the field of enzymatic preparation of structural phospholipids, and particularly relates to a method for preparing phosphatidylserine through interfacial enzyme catalysis. A series of high-activity immobilized phospholipase are prepared by innovatively adopting bifunctional group modified hollow mesoporous silica microspheres as a carrier and adsorbing immobilized phospholipase D. The catalyst has the functions of a catalyst and an emulsifier, and a Pickering emulsion interfacial enzyme catalysis system can be efficiently constructed. In the system, the phosphatidylcholine and the serine are efficiently converted into the phosphatidylserine through a phosphatidyl transfer reaction, the conversion rate of the phosphatidylserine within 20 minutes can reach 85%-95%, and the catalytic efficiency reaches up to 372 mmol / (g.h). In addition, after the catalyst is recycled for 10 times, the conversion rate is still kept at 70.9%, and the catalyst shows excellent stability and reusability. The method has the remarkable advantages of being mild in reaction condition, high in catalytic efficiency, capable of being repeatedly used and the like, and a new technical approach is provided for industrial production of phosphatidylserine.

The invention provides an application of inositol monophosphate in an enzyme catalysis reaction system for preparing inositol. The invention also provides a preparation method of the inositol, the starch is used as a substrate, glucan phosphorylase, glucose phosphate mutase, inositol-3-phosphate synthase and inositol monophosphate are added as enzyme catalysts to catalyze the substrate to generate the inositol, the inositol monophosphate is derived from Archaeoglobus fulgidus, the amino acid sequence is shown as SEQ ID NO: 12, or the amino acid sequence is shown as SEQ ID NO: 1, or the amino acid sequence is shown as SEQ ID NO: 2, or the amino acid sequence is shown as SEQ ID NO: 3, or the amino acid sequence is shown as SEQ ID NO: 4, or the amino acid sequence is shown as SEQ ID NO: 5, or the amino acid sequence is shown as SEQ ID NO: 6. The inositol monophosphate is derived from Thermococcus kodakarensis, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 13; or the inositol monophosphate is derived from Pseudothermotoga lettinae, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 14. Inositol-3-phosphate synthetase and inositol monophosphate with good performance are screened, high-concentration starch is efficiently catalyzed to be converted into inositol by a one-pot method in a whole-cell feeding mode, and NAD < + > and other coenzymes do not need to be used. Under a 60L reaction system, 200g / L of corn starch is catalyzed, the unit yield of the prepared inositol reaches 161g / L, and the method has a good industrial application prospect.

The invention discloses a 7alpha-hydroxysteroid dehydrogenase mutant with improved catalytic activity as well as a coding gene and application thereof, and the amino acid sequence of the 7alpha-hydroxysteroid dehydrogenase mutant is obtained by mutation at one or more sites of the 120th site, the 195th site and the 207th site on the basis of the amino acid sequence shown in SEQ ID NO: 1. According to the 7alpha-HSDH mutant disclosed by the invention, the key site of the 7alpha-HSDH enzyme is modified, so that the mutant with higher activity than that of a wild enzyme is obtained, the efficiency bottleneck in UDCA biosynthesis is effectively broken through, and an efficient and green enzyme catalyst is provided for industrial application.

The invention discloses application of laccase as a catalyst in catalytic synthesis of allantoin. According to the invention, laccase from thermophilus is used as an enzyme catalyst, uric acid is oxidized, hydrolyzed and decarboxylated to synthesize allantoin, and efficient biosynthesis of allantoin is realized under mild conditions; wherein the amino acid sequence of the laccase derived from the thermophilic fungi is as shown in SEQ ID NO. 1. The invention provides an efficient, environment-friendly and low-cost biosynthesis approach for biosynthesis of allantoin, and has industrial application prospects in biosynthesis of allantoin.

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The invention discloses an application of an isoeugenol monooxygenase mutant in production of isoeugenol vanillin, which comprises the steps of recombinant strain construction, fermentation culture of recombinant strains and catalytic reaction of fermentation broth, and the isoeugenol monooxygenase mutant is mutated through an isoeugenol monooxygenase amino acid sequence of wild fungi WT. And the thalli are expressed by escherichia coli, so that a series of thalli with a promoting effect on the catalytic efficiency of vanillin production are formed. The mutant super-efficient enzyme catalyst with the catalytic conversion rate reaching up to 98.5% or above is obtained through screening, the defects of low efficiency, pollution, high energy consumption and the like of traditional chemical catalyst production are overcome, and the mutant super-efficient enzyme catalyst is more suitable for green industrial production requirements and worthy of being popularized to industrial use.

The invention relates to the technical field of biology, in particular to a purification method of ergothioneine. The invention provides a method for purifying high-purity ergothioneine from an enzyme catalytic reaction. According to the method, high-purity ergothioneine is purified from the enzyme catalysis reaction liquid, so that the purification steps are greatly simplified, and the total purification yield is increased; and only a single organic solvent ethanol is used for crystallization, so that the ergothioneine product with high purity and uniform and thick particles is obtained, and the method is suitable for large-scale production of ergothioneine.

The present invention belongs to the technical field of synthesizing and preparing organic polymer optical materials, and specifically relates to a method for preparing a pentaerythritol tetramercaptocarboxylate composition. The method comprises the following two steps: first, an addition reaction of thiourea with acrylic acid under photocatalysis to generate mercaptopropionic acid in situ; and second, a lipase-catalyzed esterification reaction of mercaptopropionic acid with pentaerythritol to obtain colorless, transparent, high-purity pentaerythritol tetramercaptocarboxylate. Simultaneously, a polymerizable modified polyurethane optical material containing the pentaerythritol tetramercaptocarboxylate is prepared, characterized by low cost, high light transmittance, and adjustable refractive index.

The invention provides a glutaminase mutant and application of the glutaminase mutant. Wherein the glutaminase mutant comprises (a) a protein which is subjected to amino acid mutation and has a glutaminase activity function on at least one of the following sites of S467, Y334, K400, N521, R337, E402 or D447 of an amino acid sequence as shown in SEQ ID NO: 2, and (b) a protein which is subjected to amino acid mutation on at least one of the following sites of S334, Y334, K400, N521, R337, E402 or D447 of the amino acid sequence as shown in SEQ ID NO: 2; and (b) a protein which has 80% or more homology with the amino acid sequence limited by (a) and has a glutaminase function. The glutaminase mutant with the amino acid sequence disclosed by the invention has relatively high specific enzyme activity during enzyme catalytic reaction, and is low in cost and strong in market competitiveness when being used for commercial food processing.

The invention relates to a porphyrin iron-based biomimetic enzyme catalytic membrane for water treatment as well as preparation and application thereof, and belongs to the technical field of catalytic membranes. The porphyrin iron-based biomimetic enzyme catalytic membrane comprises a microfiltration membrane basement membrane and a porphyrin iron-based biomimetic enzyme catalyst loaded on the surface of the basement membrane in a chemical crosslinking mode, and the porphyrin iron-based biomimetic enzyme catalyst contains a catalytic activity center structure of 'Fe-N' coordination. The porphyrin iron-based biomimetic enzyme prepared by the invention is similar to a catalytic active center structure of natural peroxidase, has high affinity with hydrogen peroxide, and can greatly improve the utilization rate of hydrogen peroxide and the steady-state concentration of reactive oxygen free radicals; the constructed bionic enzyme-membrane reactor integrates dual functions of membrane separation and enzyme catalysis, realizes efficient combination of mass transfer and reaction, and greatly improves the removal performance of new pollutants in water.

The invention relates to the technical field of catalysis, and particularly discloses a method for preparing pure biodiesel from kitchen waste oil through enzyme catalytic desulfurization. The method comprises the following steps: adding a Sm2O3 / ZrO2 solid acid catalyst into the kitchen waste oil for pretreatment to obtain pretreated waste oil, and then adding lipase into the pretreated waste oil for catalyzing transesterification to obtain the biodiesel. According to the method for preparing the pure biodiesel from the kitchen waste oil through enzyme catalytic desulfurization, in-situ esterification and synchronous desulfurization of free fatty acid can be completed in a single-step reaction, the stability of a conversion reaction is effectively improved, the production process is simplified, and the conversion efficiency of preparing the biodiesel from the kitchen waste oil is improved.

The present application relates to a kind of sulfide monooxygenase mutant and its application in esomeprazole synthesis.The sulfide monooxygenase mutant, its encoding gene, the preparation method of the recombinant expression vector containing the gene sequence, co-expression recombinant vector and recombinant expression transformant, and the application of the recombinant sulfide monooxygenase mutant catalyst in esomeprazole synthesis are specifically disclosed.Compared with other sulfide monooxygenases, the sulfide monooxygenase mutant obtained by the present application can efficiently utilize the coenzyme NADH with lower cost and higher stability to catalyze the asymmetric oxidation reaction of omeprazole sulfide, prepare esomeprazole, has the advantages of high substrate concentration, mild reaction condition, environment-friendly, simple operation, high yield, low production cost and good industrial application prospect.

The invention discloses a feed low-temperature sterilization method based on a composite biological enzyme synergistic effect. The feed low-temperature sterilization method comprises the following steps: a pretreatment stage; a dynamic reaction stage: under the conditions that the temperature is 40-50 DEG C and the humidity is 60%-70%, the enzyme catalytic reaction lasts for 30 minutes, and the microbial structure is decomposed; and inactivating the activity of the residual enzyme through short-time pulsed ultraviolet rays. The compound enzyme comprises lysozyme, thermophilic protease and lipoxygenase, and the nano chitosan carrier and the compound enzyme are mixed according to the mass ratio of 1: 1. According to the method, damage to heat-sensitive nutritional ingredients is avoided through low-temperature operation (40-50 DEG C), broad-spectrum sterilization is achieved through the synergistic effect of compound enzymes, multiple microorganisms such as bacteria, molds and spores are covered, meanwhile, it is guaranteed that no chemical residues exist through the ultraviolet inactivation technology, and animal health and environment safety are guaranteed. The method is low in equipment cost, easy and convenient to operate and free of public acceptance problems, a green, efficient and economical solution is provided for the feed processing industry, and the efficiency and safety of the feed sterilization technology are remarkably improved.

The utility model discloses a fish oil distillation retort capable of increasing EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) contents, and relates to the technical field of fish oil purification. The barrel body is arranged in the tank body; the pump assembly is used for adding a catalyst in the catalyst box into the barrel; and the stirring assembly is used for stirring the fish oil, the buffer solution and the catalyst in the barrel so as to form a mixed solution. After a catalyst in the catalyst box is fed into the barrel body by the pump assembly, fish oil, a buffer solution and enzyme are stirred and mixed by the stirring assembly, so that the reaction is accelerated, the enzyme catalyst is added into the barrel body in batches, the reaction process is adjusted more accurately, waste is avoided, and the cost of the enzyme catalyst is saved; and after stirring and heating reaction for a period of time, centrifuging the obtained mixture, standing for a period of time for liquid separation, and distilling the upper oil phase to obtain the fish oil with higher EPA and DHA contents.

The invention belongs to the technical field of organic synthesis, and particularly relates to a process for catalytically synthesizing ascorbyl tetraisopalmitate by a biological enzyme. The synthesis process comprises the following steps: attaching a biological enzyme to a metal organic framework compound material (MOFs material) to prepare biological enzyme catalyst powder, and adding a small amount of biological enzyme catalyst powder into methyl n-hexylcaprate and ascorbic acid which are used as raw materials for transesterification to prepare ascorbyl tetraisopalmitate. The process route is simple, few three wastes are generated, compared with a traditional preparation process of tetra-isopalm ascorbate, the yield and purity of the product are greatly improved, and experimental results show that the highest yield of the product is 89%, and the purity of the product reaches 99.0%.

The invention discloses a resourceful treatment process for phenol-ammonia wastewater. The process comprises the following steps: pretreating to remove oil, acid and ammonia, and recovering ammonia water; preparing a silicon-based supported phenol oxidase catalyst; carrying out enzyme catalysis polycondensation on the pretreated wastewater to generate polyphenols; residual phenol is deeply removed through electrochemical feeding of the three-dimensional electrode; after polyphenol substances are separated, supernate is subjected to biochemical treatment and is discharged after reaching the standard; and finally, carrying out dehydration, granulation, segmented pyrolysis graphitization and post-treatment on the collected polyphenol sludge to prepare the carbon negative electrode material. According to the method, deep purification of the phenol-ammonia wastewater and collaborative recycling of pollutants are achieved, toxic phenols are converted into carbon material precursors with high additional value, the recycled ammonia water can be used for desulfurization and denitrification of a boiler, and the method has the advantages of being thorough in treatment, high in resource recovery rate, low in cost and remarkable in environmental benefit.

The invention relates to the technical field of enzyme catalytic reaction equipment, and discloses a transglucosidase catalytic reaction device capable of avoiding infectious microbe infection.The transglucosidase catalytic reaction device comprises a catalytic reaction device body, a motor body is installed on the catalytic reaction device body, and the output end of the motor body is connected with a stirring shaft through a coupler; a motor body is started to drive a stirring shaft, meanwhile, under the cooperation of a transmission assembly, an overturning scraping plate on a cleaning scraping base can do circular motion, residual materials attached to the inner wall of the catalytic reaction device body can be scraped away, and when materials continue to be put into the catalytic reaction device body, the materials can be automatically cleaned by starting a second electric telescopic rod, so that the materials can be automatically cleaned. And meanwhile, under the cooperation of a disinfection cleaning assembly, the overturning scraping plate with impurity cakes is overturned into a cleaning scraping base to be subjected to isolation cleaning, if residual materials on the inner wall are attached for a long time, the residual materials can chemically react with the inner wall of the reaction device, the inner wall is corroded and abraded, and the structural integrity and performance stability of equipment are affected.

The present invention discloses a mutant dual-enzyme catalyst, application and (S)-Bosin synthesis method, relating to the field of bioengineering technology, the present invention is based on the methylglyoxal reductase GRE2 (A) of Candida glabrata and the phosphite dehydrogenase PTDH of methylotrophic bacteria as sources for transformation, and its mutants are screened, the key residues affecting the enzymatic properties of the initial methylglyoxal reductase and phosphite dehydrogenase are determined, and methylglyoxal reductase mutants and phosphite dehydrogenase mutants with high activity are obtained. The present invention utilizes the two most efficient mutants to catalyze the reaction of preparing (S)-Bosin from β-acetone xyloside in a 22T reaction system, with good catalytic effect, overall catalytic efficiency is increased by about 43%, and cost is reduced by about 8%.

The invention belongs to the field of biochemical engineering, and particularly relates to a method for preparing xylitol through continuous catalysis of immobilized enzyme coupled ion exchange, which specifically comprises the following steps: co-immobilizing xylose reductase, glucose dehydrogenase and NADP + coenzyme, producing xylitol through enzyme catalysis by taking xylose and glucose as raw materials, and coupling an ion exchange reaction after the enzyme catalysis reaction to obtain the xylitol. Due to the arrangement of the ion exchange reaction, the byproduct gluconate can be separated, and the pH of the reaction liquid can be regulated and controlled by using alkaline anions exchanged in the ion exchange process, so that the normal operation of the reaction is further ensured; the xylitol and the by-product gluconate obtained by the reaction can be separated without an additional separation process. The method provided by the invention successfully solves the problems of complex process, high separation difficulty and high cost in the process of refining xylitol by a biological method.

The utility model provides a continuous flow micro-reactor for a heparinase catalytic reaction, belongs to the technical field of micro-reactors, and aims to solve the problems that the replacement of immobilized heparinase needs a long time and the efficiency of the heparinase catalytic reaction is influenced. Comprising a limiting supporting base, a positioning mounting frame, a reactor main body, a positioning connecting rod, a first storage tank and a second storage tank, the positioning mounting frame is in bolted connection with the right side of the limiting supporting base; the reactor main body is fixedly connected to the left side of the positioning mounting frame; the positioning connecting rod is rotationally connected to the left side of the reactor main body, and a damper is arranged at the joint of the positioning connecting rod and the reactor main body; the first storage tank is fixedly connected to the middle part of the positioning connecting rod; the second storage tank is in bolted connection with the front side of the first storage tank; according to the utility model, the replacement efficiency of the immobilized heparinase is improved, a large amount of time is saved, and the catalytic reaction efficiency of the heparinase is improved.

The invention discloses a desaturase TgDH. The desaturase TgDH has an amino acid sequence as shown in SEQ ID No. 2 or SEQ ID No. 3. The desaturase TgDH is used as a biocatalyst, and the chiral ketene compound can be synthesized with high yield and high stereoselectivity. A catalytic asymmetric technical route of the enzyme is mild in reaction condition, environment-friendly and high in selectivity, the catalyst is cheap and easy to obtain, separation and purification are simple and convenient, the optical purity is greater than 99% ee, and the actual separation yield (77%) is relatively high. The invention discloses a method for asymmetrically synthesizing a chiral 4-substituted cyclohexenone compound, which takes a 4-substituted cyclohexanone compound as a substrate, takes desaturase TgDH or whole cells containing the desaturase TgDH as a catalyst and reacts in a solvent to obtain the chiral 4-substituted cyclohexenone compound.

The application discloses a laccase-producing polyporus brumalis and a method for preparing laccase. The application provides polyporus brumalis CCTCC NO: M 2020809 for fermenting and preparing laccase, and the amino acid sequence of the laccase is shown as SEQ ID NO: 2. The fermentation level of the laccase is high, the fermentation level in a flask reaches 980 U / mL, and the fermentation level in a 70L fermenter reaches 880 U / mL. In order to ensure the food safety of the laccase, the strain and the preferred medium components of the laccase are edible. In addition, the laccase obtained by the application can catalyze the polymerization of film-forming monomers to generate a copolymer film, and is a method for synthesizing a copolymer film by using a biological enzyme catalyst.

The invention discloses a biological catalytic synthesis method of chiral 2-amino-1-methylenepropanamide and an enzyme catalyst of the chiral 2-amino-1-methylenepropanamide. According to the present invention, one or more key amino acids in the amide hydrolase derived from Rhodococcus erythropolis AJ270 are subjected to mutation to obtain the mutant, the mutant has characteristics of high or reverse enantioselectivity when the hydrolysis reaction of the racemization 2-substituted-2-amino-1-methylene propanamide substrate is catalyzed, and the chiral compound is obtained with the high ee value; and an enantiomer of a wild type amide hydrolase catalytic product can be obtained. A method for preparing chiral 2-substituted-2-amino-1-methylene propanamide by using the mutant through biological catalysis has the characteristics of simplicity and convenience in operation, high reaction efficiency, mild reaction conditions, high enantioselectivity, easiness in product separation and high product purity, and has a very good application prospect.

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

The invention relates to water-soluble polysiloxane as well as a preparation method and application thereof. The preparation method of the water-soluble polysiloxane comprises the following steps: mixing lipase, trehalose and a surfactant to prepare modified lipase; vinyl acetate, alkoxy silane, monomethoxy polyethylene glycol and the modified lipase are mixed, and the water-soluble polysiloxane is prepared. According to the preparation method, the lipase is coated with the trehalose to form the modified lipase, so that the modified lipase can keep efficient catalytic activity for a long time at a relatively high temperature, and further the water-soluble polysiloxane can be efficiently prepared through a lipase catalytic reaction one-step method. Compared with a traditional acid / alkali synthesis method, the preparation method of the water-soluble polysiloxane can obtain a higher conversion rate at a lower reaction temperature, meanwhile, an antioxidant does not need to be added, and the obtained water-soluble polysiloxane is good in water solubility, light transmittance and safety.

The present invention relates to the preparation and use of high-entropy nanozymes with multi-pathway synergistic antibacterial effects, belonging to the field of nanozyme catalytic materials. The present invention, for the first time, utilizes a hydrothermal method and high-temperature calcination method to synthesize oxygen-vacancy-rich high-entropy oxides with haloperoxidase-like, peroxidase-like, and glutathione oxidase-like activities. These oxides can catalyze bromide ions and hydrogen peroxide to generate hypobromous acid, which interferes with bacterial quorum sensing, and hydroxyl radicals, which disrupt structure and function. Furthermore, these oxides consume glutathione, which maintains the internal redox balance of bacteria. This allows for multi-pathway synergistic and efficient bacterial damage and inactivation, helping to address the problems of existing antifouling nanozyme materials, which suffer from a single catalytic pathway and poor component adjustability. This allows for the practical expansion of high-entropy nanozyme coatings, and has potential application prospects in the field of marine biofouling.

The invention relates to the technical field of wind power materials, and discloses a bio-based matrix, a bio-based resin composite material and a preparation method and application of the bio-based matrix and the bio-based resin composite material. The bio-based resin matrix is prepared by carrying out polymerization reaction on vegetable oil under the action of an enzyme catalyst; and an enzyme catalysis-nanometer enhancement synergistic technology is used, so that the problems of high viscosity of the bio-based resin and high modulus and poor water resistance of the composite material are solved at the same time.

The invention discloses a batch-type enzyme catalytic reaction method, which adopts a batch-type enzyme catalytic reaction device, the batch-type enzyme catalytic reaction device comprises a reaction kettle and a static mixer, and the reaction kettle and the static mixer are connected through an external circulation pipeline to form a closed loop; a reaction system circulates in a closed loop, and enzyme catalysis reaction is carried out at the same time; the reaction system comprises a reaction substrate and an enzyme catalysis system. By adopting the method, the enzyme catalytic reaction rate can be obviously improved, the production cost can be reduced, and the conversion rate can be improved.

The invention belongs to the field of polishing solutions, and particularly relates to a green polishing solution based on an enzymatic Fenton system. The 4H-SiC substrate polishing solution comprises a component A and a component B, the effective component of the component A is an enzyme-catalyst co-immobilized abrasive material, and the enzyme-catalyst co-immobilized abrasive material comprises an abrasive material matrix, laccase and an iron-based catalyst, and the laccase and the iron-based catalyst are loaded on the abrasive material matrix; the active ingredient of the component A is a phenol substrate, the active ingredient of the component B is a phenol substrate, after the component A and the component B are mixed, laccase catalyzes oxidation of the phenol substrate to generate H2O2 in situ, the H2O2 and an iron-based catalyst are subjected to a Fenton reaction, and hydroxyl free radicals are generated at a polishing interface in situ. According to the invention, continuous, efficient and stable supply of hydroxyl radicals is ensured through enzymatic endogenous hydrogen peroxide circulation and catalyst regeneration circulation, so that the polishing solution can realize long-time stable polishing under a nearly neutral condition, and has the advantages of environmental protection, high polishing efficiency, good surface quality and the like.