180 results about "Enzyme catalyzed" patented technology

The invention relates to the technical field of biological catalysis, and discloses an L379A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: leucine of the 379th amino acid sequence is mutated into alanine; the enzyme can be applied to RA in-vitro conversion preparation of RI with higher utilization value, the conversion rate is higher than 50%, and the enzyme activity is remarkably improved by 7 times compared with the original enzyme catalytic conversion enzyme activity. The UGT76G1 mutant disclosed by the invention has the advantages that (1) the blank is filled, and a special enzyme catalyst capable of realizing efficient in-vitro synthesis of rebaudioside I (RI) is provided for the first time; 2) efficiency jump: the catalytic conversion rate is greatly increased from original about 7% to more than 50% (increase gt; and 3) stability and reliability: in the cross-scale reaction of 10mL to 5L, the catalyst has stable catalytic performance, shows excellent industrial application potential, and is suitable for popularization and application.

The invention provides an application of inositol monophosphate in an enzyme catalysis reaction system for preparing inositol. The invention also provides a preparation method of the inositol, the starch is used as a substrate, glucan phosphorylase, glucose phosphate mutase, inositol-3-phosphate synthase and inositol monophosphate are added as enzyme catalysts to catalyze the substrate to generate the inositol, the inositol monophosphate is derived from Archaeoglobus fulgidus, the amino acid sequence is shown as SEQ ID NO: 12, or the amino acid sequence is shown as SEQ ID NO: 1, or the amino acid sequence is shown as SEQ ID NO: 2, or the amino acid sequence is shown as SEQ ID NO: 3, or the amino acid sequence is shown as SEQ ID NO: 4, or the amino acid sequence is shown as SEQ ID NO: 5, or the amino acid sequence is shown as SEQ ID NO: 6. The inositol monophosphate is derived from Thermococcus kodakarensis, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 13; or the inositol monophosphate is derived from Pseudothermotoga lettinae, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 14. Inositol-3-phosphate synthetase and inositol monophosphate with good performance are screened, high-concentration starch is efficiently catalyzed to be converted into inositol by a one-pot method in a whole-cell feeding mode, and NAD < + > and other coenzymes do not need to be used. Under a 60L reaction system, 200g / L of corn starch is catalyzed, the unit yield of the prepared inositol reaches 161g / L, and the method has a good industrial application prospect.

The invention discloses a 7alpha-hydroxysteroid dehydrogenase mutant with improved catalytic activity as well as a coding gene and application thereof, and the amino acid sequence of the 7alpha-hydroxysteroid dehydrogenase mutant is obtained by mutation at one or more sites of the 120th site, the 195th site and the 207th site on the basis of the amino acid sequence shown in SEQ ID NO: 1. According to the 7alpha-HSDH mutant disclosed by the invention, the key site of the 7alpha-HSDH enzyme is modified, so that the mutant with higher activity than that of a wild enzyme is obtained, the efficiency bottleneck in UDCA biosynthesis is effectively broken through, and an efficient and green enzyme catalyst is provided for industrial application.

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The invention relates to the technical field of catalysis, and particularly discloses a method for preparing pure biodiesel from kitchen waste oil through enzyme catalytic desulfurization. The method comprises the following steps: adding a Sm2O3 / ZrO2 solid acid catalyst into the kitchen waste oil for pretreatment to obtain pretreated waste oil, and then adding lipase into the pretreated waste oil for catalyzing transesterification to obtain the biodiesel. According to the method for preparing the pure biodiesel from the kitchen waste oil through enzyme catalytic desulfurization, in-situ esterification and synchronous desulfurization of free fatty acid can be completed in a single-step reaction, the stability of a conversion reaction is effectively improved, the production process is simplified, and the conversion efficiency of preparing the biodiesel from the kitchen waste oil is improved.

The invention discloses a resourceful treatment process for phenol-ammonia wastewater. The process comprises the following steps: pretreating to remove oil, acid and ammonia, and recovering ammonia water; preparing a silicon-based supported phenol oxidase catalyst; carrying out enzyme catalysis polycondensation on the pretreated wastewater to generate polyphenols; residual phenol is deeply removed through electrochemical feeding of the three-dimensional electrode; after polyphenol substances are separated, supernate is subjected to biochemical treatment and is discharged after reaching the standard; and finally, carrying out dehydration, granulation, segmented pyrolysis graphitization and post-treatment on the collected polyphenol sludge to prepare the carbon negative electrode material. According to the method, deep purification of the phenol-ammonia wastewater and collaborative recycling of pollutants are achieved, toxic phenols are converted into carbon material precursors with high additional value, the recycled ammonia water can be used for desulfurization and denitrification of a boiler, and the method has the advantages of being thorough in treatment, high in resource recovery rate, low in cost and remarkable in environmental benefit.

The utility model provides a continuous flow micro-reactor for a heparinase catalytic reaction, belongs to the technical field of micro-reactors, and aims to solve the problems that the replacement of immobilized heparinase needs a long time and the efficiency of the heparinase catalytic reaction is influenced. Comprising a limiting supporting base, a positioning mounting frame, a reactor main body, a positioning connecting rod, a first storage tank and a second storage tank, the positioning mounting frame is in bolted connection with the right side of the limiting supporting base; the reactor main body is fixedly connected to the left side of the positioning mounting frame; the positioning connecting rod is rotationally connected to the left side of the reactor main body, and a damper is arranged at the joint of the positioning connecting rod and the reactor main body; the first storage tank is fixedly connected to the middle part of the positioning connecting rod; the second storage tank is in bolted connection with the front side of the first storage tank; according to the utility model, the replacement efficiency of the immobilized heparinase is improved, a large amount of time is saved, and the catalytic reaction efficiency of the heparinase is improved.

The invention belongs to the technical field of biology, and particularly relates to a pentachloronitrobenzene rapid detection method based on enzyme catalysis reaction, which comprises the following steps: converting pentachloronitrobenzene into pentachlorophenol and nitrate radicals through an enzyme system consisting of iron thioflavin protein reductase, ferredoxin and nitrobenzene dioxygenase; and then nitrate radicals are specifically detected by a Griess reagent method so as to indirectly reflect quintozene. The detection method disclosed by the invention is good in specificity, short in detection time and remarkable in visual detection effect, and can be used for on-site rapid detection of quintozene.

The invention relates to water-soluble polysiloxane as well as a preparation method and application thereof. The preparation method of the water-soluble polysiloxane comprises the following steps: mixing lipase, trehalose and a surfactant to prepare modified lipase; vinyl acetate, alkoxy silane, monomethoxy polyethylene glycol and the modified lipase are mixed, and the water-soluble polysiloxane is prepared. According to the preparation method, the lipase is coated with the trehalose to form the modified lipase, so that the modified lipase can keep efficient catalytic activity for a long time at a relatively high temperature, and further the water-soluble polysiloxane can be efficiently prepared through a lipase catalytic reaction one-step method. Compared with a traditional acid / alkali synthesis method, the preparation method of the water-soluble polysiloxane can obtain a higher conversion rate at a lower reaction temperature, meanwhile, an antioxidant does not need to be added, and the obtained water-soluble polysiloxane is good in water solubility, light transmittance and safety.

The invention relates to the technical field of wind power materials, and discloses a bio-based matrix, a bio-based resin composite material and a preparation method and application of the bio-based matrix and the bio-based resin composite material. The bio-based resin matrix is prepared by carrying out polymerization reaction on vegetable oil under the action of an enzyme catalyst; and an enzyme catalysis-nanometer enhancement synergistic technology is used, so that the problems of high viscosity of the bio-based resin and high modulus and poor water resistance of the composite material are solved at the same time.

The invention belongs to the field of polishing solutions, and particularly relates to a green polishing solution based on an enzymatic Fenton system. The 4H-SiC substrate polishing solution comprises a component A and a component B, the effective component of the component A is an enzyme-catalyst co-immobilized abrasive material, and the enzyme-catalyst co-immobilized abrasive material comprises an abrasive material matrix, laccase and an iron-based catalyst, and the laccase and the iron-based catalyst are loaded on the abrasive material matrix; the active ingredient of the component A is a phenol substrate, the active ingredient of the component B is a phenol substrate, after the component A and the component B are mixed, laccase catalyzes oxidation of the phenol substrate to generate H2O2 in situ, the H2O2 and an iron-based catalyst are subjected to a Fenton reaction, and hydroxyl free radicals are generated at a polishing interface in situ. According to the invention, continuous, efficient and stable supply of hydroxyl radicals is ensured through enzymatic endogenous hydrogen peroxide circulation and catalyst regeneration circulation, so that the polishing solution can realize long-time stable polishing under a nearly neutral condition, and has the advantages of environmental protection, high polishing efficiency, good surface quality and the like.

The application uses cheap and easily available hexose acid as a starting material, first obtains two precursors of 2,5-furan dicarboxylic acid synthesis, namely hexahydroxy acid and 4-deoxy-5-dehydro-hexahydroxy acid through enzyme catalytic reaction, and then 4-deoxy-5-dehydro-hexahydroxy acid is dehydrated under acidic conditions to obtain 2,5-furan dicarboxylic acid.

The invention provides a method for continuously preparing an acrylamide aqueous solution by using a core-shell structure immobilized enzyme, and relates to the field of chemical engineering, the preparation method comprises the following steps: simultaneously introducing an acrylonitrile stock solution and water into a catalytic reaction unit for hydration reaction; the reaction temperature of the catalytic reaction unit is 10-20 DEG C; enabling reaction effluent to enter a primary purification unit, a deep purification unit and a terminal purification unit to obtain an acrylamide aqueous solution; wherein the catalytic reaction unit is a fixed reactor filled with a core-shell structure enzyme catalyst. The method has the advantages of few product impurities, low energy consumption and high safety.

The invention relates to a method for producing esters by reaction between a first reagent chosen from an alcohol or a carboxylic acid, contained in an aqueous solution, and at least one second reagent chosen from a carboxylic acid or an alcohol, in the presence of an enzymatic catalyst. The invention is characterised in that the reaction mixture is in the form of a Pickering emulsion comprising at least one water phase and one oil phase, said oil phase comprising at least one extraction solvent.

This invention relates to the technical field of imiquimod synthesis, specifically to catalysts, methods, and applications for imiquimod synthesis. The invention employs a lipase-immobilized lipase complex carrier to catalyze a transesterification reaction, using vinyl isobutyrate as the acyl donor and tert-butanol as the solvent. The hydroxyl group of 3-(4-amino-1H-imidazo[4,5-C]quinoline-1-yl)-2-methylpropane-1-ol is converted to an isobutyl side chain. Following enzymatic catalysis and pH adjustment, hydrolysis is performed to release the isobutyl group, further generating imiquimod and isobutyric acid. The lipase selectively catalyzes transesterification, avoiding side reactions, and the ester bond is rapidly hydrolyzed to the target product under acidic conditions.

The present application relates to the technical field of polymer material chemical recycling, and in particular to a kind of nano-enzyme catalyst and process for its use in hydrothermal method degradation polycaprolactam.The nano-enzyme catalyst provided by the present application uses specific single metal or double metal combination as metal center, and is combined with specific organic ligand, can be efficiently and deeply degraded PA6 at relatively low temperature, and the catalyst can also be recycled, completely realizing the green recycling of PA6.The nano-enzyme catalyst provided by the present application can make the conversion rate of PA6 molecule reach 100% using hydrothermal method, and the caprolactam yield can be stably above 94%, avoiding the use of organic solvent with high toxicity, and realizing the whole green recycling from " waste containing PA6" to " renewable caprolactam monomer".

The invention discloses a method for rapidly detecting acrylamide in food by using a fluorescent probe, which comprises the following steps of: synthesizing copper and iron-containing phosphomolybdic acid nano-enzyme (Cu, Fe-PMos) with peroxidase-like nano-enzyme activity by using phosphomolybdic acid, copper chloride and ferric chloride as precursors through a microwave method, catalyzing xanthol to react with HCl to generate a fluorescent derivative by using the Cu and Fe-PMos, and detecting the acrylamide in the food by using the fluorescent probe. When acrylamide is added into a fluorescent system, reaction of xanthene alcohol and acrylamide is carried out to generate a xanthene acrylamide fluorescent substance, reaction of Cu, Fe-PMos and xanthene alcohol is inhibited, and the fluorescence intensity of the system is reduced, so that a fluorescent probe rapid detection method of acrylamide is established. Other coexisting substances do not interfere with each other, the detection limit reaches 0.01 mu g / mL, and the method has the characteristics of high sensitivity, strong specificity, simplicity and rapidness in operation and the like.

The present application relates to the technical field of genetic engineering, and particularly provides a Diels-Alder reaction enzyme mutant as well as a preparation method and application thereof. The present application relates to functional modification and strengthening of a Diels-Alder (D-A) reaction enzyme MaDA1 derived from mulberry, so that the enzyme catalyzes intermolecular D-A reaction of a non-natural dienophile containing a ''handle group'' (carboxyl, ester or a precursor thereof) and various dienophiles, so that further modification of the enzymatic D-A product can be realized through decarboxylation functionalization reaction, and diversification and stereoselective synthesis of a cyclohexene skeleton structure are realized.

The present application relates to the field of electroenzymatic organic synthesis, in particular to a rhodium-based catalyst and a preparation method and use thereof, the rhodium-based catalyst has the method of electrocatalytic reduction of CO2 to formic acid and the use thereof in the coupling multi-enzyme cascade reaction for producing high-value-added chemicals from CO2. The present application provides a rhodium-based catalyst ([Cp*Rh(bpy)H2O] 2+ ) and a method for simultaneously electrocatalytically reducing CO2 to formic acid and reducing oxidized coenzyme I (NAD + ) to obtain reduced coenzyme I (NADH). The above-mentioned rhodium-based catalyst effectively solves the limitation of low activity and low efficiency of enzymatic reduction of carbon dioxide to formic acid, and at the same time lays the foundation for providing necessary reducing power (NADH) for subsequent multi-enzyme cascade, and finally realizes high-added chemicals directly from CO2.

The application belongs to the technical field of polymer material recycling and catalytic chemistry, and discloses a composite catalyst for PET waste bottle alcoholysis and a preparation method thereof. The composite catalyst is based on biomimetic enzyme engineering and dynamic covalent chemistry strategy, and comprises a binuclear metal cluster active center, a limited microenvironment shell layer and a dynamic covalent network skeleton. The binuclear metal cluster is constructed by zinc / cobalt / manganese ions and a tridentate ligand C9H8N2O4, the shell layer is a polydopamine derived functional polymer, and the skeleton is formed by cross-linking aromatic and aliphatic monomers through borate ester bonds. The application solves the performance degradation problem caused by the instability of the active center of the existing enzyme-like catalyst in industrial circulation application. The catalyst not only has the enzyme-like catalytic characteristics of high activity and high selectivity, but also realizes self-repairing after damage through the built-in multiple dynamic chemical mechanism, thereby providing a new catalytic material with performance and durability for efficient and sustainable chemical recycling of PET waste bottles.

The invention provides a method for synthesizing a sialic acid oligosaccharide library with various sulfonated structures by a chemical enzyme method, and researches on the binding activity of sialic acid binding immunoglobulin-like lectin (Siglecs). Specifically, sialic acid and 9-azido-sialic acid are used as chemical-like protecting groups through an enzyme-catalyzed sialylation reaction, a specific position of oligosaccharide is protected, and then a sulfonate group is introduced at a preset site by utilizing high efficiency of a chemical reaction. Sialic acid or 9-azido-sialic acid, which is used as a protecting group, is removed by sialyl hydrolase, so that a sulfated oligosaccharide framework structure is obtained. And finally, carrying out enzymatic extension to obtain sulfonated sialic acid oligosaccharides with diversified structures. By means of the strategy, 66 kinds of sulfonated sialic acid oligosaccharides can be rapidly and efficiently prepared, the oligosaccharide chip prepared from the sulfonated sialic acid oligosaccharides is used for conducting systematic high-throughput activity screening on 12 proteins in the Siglecc family, and the high-affinity glycosyl ligands of the Siglecc proteins are found through research.

Processes for transesterifying wax esters. Implementations may include: providing a feedstock including wax esters, contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. An oxidative stability index (OSI) of the transesterified product may be greater than an OSI of the feedstock.

The invention discloses an enzymatic synthesis method of a melogabalin intermediate and related alcohol dehydrogenase, racemic 3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone is used as a substrate, a genetically engineered bacterium for producing alcohol dehydrogenase is used as an enzyme catalyst, a genetically engineered bacterium for producing formate dehydrogenase, ammonium formate and coenzyme are matched, an enzymatic reaction is carried out under a stirring condition, and the melogabalin intermediate is obtained. And carrying out purification and separation on a reaction product to obtain the melogabalin intermediate (1R, 5S)-3-ethyl bicyclo [3.2. 0] hept-3-ene-6-ketone. The method has the advantages of simple reaction, few steps, mild reaction, less pollution and the like, and has great industrial application potential and commercial value.

Processes for transesterifying wax esters. Implementations may include: providing a feedstock including wax esters, contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. An oxidative stability index (OSI) of the transesterified product may be greater than an OSI of the feedstock.

The application belongs to the technical field of biochemical synthesis, and particularly relates to a high stereoselectivity amino-cleaving enzyme and a synthesis method of (S)-3-aminobutyronitrile and a salt thereof. The high stereoselectivity amino-cleaving enzyme is selected from the amino acid sequences shown in SEQ ID NO. 6-8. The synthesis method comprises: performing an enzyme catalytic reaction on crotonic acid in the presence of the high stereoselectivity amino-cleaving enzyme to obtain (S)-3-aminobutyric acid with a chiral purity e.e. of 99.5% or more; reacting (S)-3-aminobutyric acid with di-tert-butyl dicarbonate, and then reacting with ammonium bicarbonate, and further performing dehydration and deprotection to obtain (S)-3-aminobutyronitrile hydrochloride. The application combines chemical method and enzyme method, avoids the use of highly toxic substances, is safe and efficient, improves the economic benefits in the industrialization process, and the overall route reflects the value of green chemical synthesis.

The utility model discloses a continuous enzyme catalytic reaction tank, which relates to the technical field of enzyme catalytic reaction and comprises a support base frame, a reaction tank is arranged in the middle of the support base frame, a top cover is arranged at the top of the reaction tank, a mixing component is arranged in the middle of the top cover, a partition component is arranged in the reaction tank, and the partition component is connected with the support base frame. The mixing assembly comprises a driving motor, a rotating rod is arranged at the bottom of the driving motor, a mounting ring is arranged outside the rotating rod, and stirring paddles are arranged on the outer wall of the mounting ring. The immobilized enzyme and the reaction liquid are separated through the separation assembly, the immobilized enzyme is blocked through the separation frame and the filter screen, so that the immobilized enzyme is conveniently collected and treated in a centralized mode, the use convenience of the device is improved, the separation frame and the filter screen are located in the middle of the reaction tank, and the separation effect is good. The full reaction of the immobilized enzyme and the reaction liquid is facilitated, and the practicability of the device is improved.

The invention discloses an isosorbide polymerizable chiral agent and a preparation method thereof. The preparation method comprises the following steps: mixing isosorbide, a benzoyl chloride derivative, 3-phenylpyridine, absolute ethyl alcohol, potassium hydroxide and a lipase catalyst, and uniformly stirring; under the anaerobic condition, microwave irradiation is utilized to initiate a reaction, and ultraviolet irradiation and laser irradiation are used while microwave irradiation is started; the microwave frequency is 2350-2500MHz, the microwave power is 400-1500W, the ultraviolet wavelength is 190-380nm, the illumination intensity is 5mW / cm < 2 >-5W / cm < 2 >, the temperature is 20-100 DEG C, and irradiation is carried out for 0.5-12h to prepare the liquid crystal polymer which can be used as a chiral compound, can be polymerized, has a strong HTP value and a low melting point, has excellent dissolvability with other liquid crystal compounds, can be used as a constituent material of a polymerizable liquid crystal polymer, has large twisting force, and can be used for preparing the liquid crystal polymer. The optical anisotropic body with excellent optical characteristics can be prepared, the raw materials are cheap and easy to obtain, the preparation method is simple, and the product purity is high.

The application discloses a method for preparing 2,6-dimethyl-2,3-dihydro-1-indanone or a mixture of any proportion isomer thereof, comprising the following steps: 1) reacting 2,6-dimethyl-2,3-dihydro-1H-inden-1-amine or a mixture of any proportion isomer thereof with a halogenated reagent to obtain a mixture containing an intermediate; 2) separating or not separating the intermediate, and reacting the intermediate with a reducing agent to obtain 2,6-dimethyl-2,3-dihydro-1-indanone. The application can convert 2,6-dimethyl-2,3-dihydro-1H-inden-1-amine from an amine into a ketone, especially convert an indanamine isomer which cannot be catalyzed by a lipase to be acylated into a ketone, realizes recycling of raw materials, and greatly reduces raw material cost. The halogenated reagent can be synthesized in situ or used, a process is saved, activity of the halogenated reagent is improved, and meanwhile, raw material cost is also lower.

A poly(PEG-co-PFPE) or poly(PPG-co-PFPE) copolymer or hetero-oligomer capable of dissolving > 5% (m / m) of a sodium salt, such as NaTFSI. When mixed with a sodium salt, a polymer electrolyte (PE) is formed. The hetero-oligomer is formed by mixing PEG or PPG with PEPE in the presence of a Candida antarctica lipase enzymatic catalyst and heating the mixture and isolating the resulting hetero-oligomer from the lipase catalyst.

This invention discloses a method for the enzymatic synthesis of L-aminobutyric acid (GABA), comprising the following steps: using threonine and ammonium formate as substrates, a three-enzyme combined catalytic reaction is carried out using leucine dehydrogenase, threonine deaminase, and formate dehydrogenase at a temperature of 40-55°C and a pH of 7.0-9.0 to obtain L-aminobutyric acid. The leucine dehydrogenase is the leucine dehydrogenase with the amino acid sequence shown in SEQ ID NO:1 or a mutant thereof. This invention can rapidly convert threonine to L-aminobutyric acid, yielding L-aminobutyric acid with high optical purity, thus improving economic efficiency.