The invention relates to a top-bottom asymmetrical tert-butyl spirobifluorene compound. The compound of the invention has a structural formula as represented by (I), and in the structural formula, R1 can be any one of various electron-withdrawing groups and electron-donating groups, such as a variety of alkyl groups, substituted aryl groups, substituted heterocyclic radicals, substituted alkylene groups, substituted amino groups, chlorine, bromine and iodine, except tert-butyl groups. According to the invention, cheap and easily available 4,4'-di-tert-butyl-biphenyl is used a raw material and reacts with 2,7-biX-fluorenone (wherein X represents substituents) through bromination, X substituents on a fluorene ring are converted into a variety of functional groups, and other steps are also carried out so as to obtain the compound (I). The top-bottom asymmetrical tert-butyl spirobifluorene compound in the invention has the characteristics of low cost, a simple synthesis route, high yield, easiness in industrial production, a special spiro-conjugation effect, good dissolvability, high thermal stability and a wide applicability in the fields of organic light-emitting displays, organic non-linear materials, fluorescent probes and the like.