101 results about "D-GLUCURONIC ACID" patented technology

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention provides application of pitaya peel polysaccharide in preparation of a medicine for repairing skin wounds. The monosaccharide components of the pitaya peel polysaccharide comprise rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. Research finds that the dragon fruit peel polysaccharide remarkably accelerates the wound healing process and promotes granulation tissue formation and collagen deposition. Meanwhile, the pitaya peel polysaccharide can also promote cell proliferation and neovascularization at the skin wound part, exert an anti-inflammatory effect, remodel skin microbiota and provide a more suitable microenvironment for skin wound healing. The invention provides a new drug choice for clinically repairing skin wounds, and can be used for developing new skin wound dressings.

The invention discloses application of GUX1 protein and related biological materials thereof in regulation and control of xylan glucuronic acid modification. Through overexpression of GUX1 in rice and tobacco, the invention finds that the short interval substitution of xylan glucuronic acid in an acceptor material is specifically increased. According to the invention, the function of short-interval glucuronic acid addition of GUX1 mediated xylan is found for the first time, and the GUX1 gene can be used for regulating a glucuronic acid modification mode of plant xylan and producing a corresponding xylan structure product, and is an important and valuable gene for cell wall structure and function modification.

This invention belongs to the field of photocrosslinking probe technology, specifically relating to a GUSB photocrosslinking probe, its preparation method, and its application. The GUSB photocrosslinking probe has the general chemical formula C0. 13 H 18 N2O6-R, wherein R is any one of the oxalic group elements or peptide bonds. The R group covalently connects the glucuronic acid recognition core and the photosensitive unit in the GUSB photocrosslinking probe, and the R group contains no more than 4 atoms, resulting in a small size and low steric hindrance. The GUSB photocrosslinking probe provided by this invention uses glucuronic acid as the recognition core, combined with a small-volume diazacyclopropane photosensitive unit and a terminal alkyne group, to achieve µM-level in-situ covalent capture of active GUSB in complex systems such as cells or tissues. The probe of this invention significantly improves positioning accuracy, sensitivity, data availability, quantification, and enrichment, while also possessing advantages such as modular synthesis, mild conditions, and strong system compatibility, effectively addressing the technical shortcomings of existing technologies that struggle to accurately obtain GUSB activity in situ under low-dose conditions.

The present application relates to the technical field of medicine, and provides a kind of haena polysaccharide and its preparation method and application and target endogenous coagulation pathway anticoagulant and / or antithrombotic drug.The haena polysaccharide provided by the present application is fucosylated chondroitin sulfate polysaccharide, the weight average molecular weight is 9-13 million, the molar ratio of glucuronic acid, N-acetylglucosamine and fucose is 1:0.8-1.2:0.5-0.8, and the mass percentage content of sulfate group is 25-40%.The haena polysaccharide provided by the present application can target endogenous coagulation pathway terminal rate-limiting enzyme (iFXase), and has no obvious effect on coagulation factors in other coagulation pathways, realizes the effect of anticoagulation and non-hemorrhage, and can be widely applied in acute and recovery period treatment of ischemic stroke, solves the safety problem of using anticoagulant and antithrombotic drug for clinical ischemic stroke patients, and opens up a new field of research and development of target endogenous coagulation pathway rate-limiting enzyme anticoagulant drug.

The application discloses a glucuronolactone and a preparation method thereof, and belongs to the technical field of fine chemical product preparation. The method takes potassium phytate extracted from corn soaking liquid or rice bran acid soaking liquid as raw material, obtains inositol and potassium dihydrogen phosphate after hydrolysis, separates the hydrolysis liquid through chromatography, obtains potassium dihydrogen phosphate by concentrating and crystallizing the salt phase, and the material phase is inositol solution. Enzymatic conversion reaction is carried out by using fermentation to obtain enzyme liquid, glucuronic acid liquid is obtained, glucuronolactone is obtained by concentrating and esterifying, and then the glucuronolactone is obtained by concentrating, refining and crystallizing. In the method, the yield of potassium phytate to inositol is greater than 95%, the molar conversion rate of inositol enzymatic conversion to glucuronic acid is greater than 95%, and the total yield is greater than 89%, which is much higher than the traditional yield, and the content of the obtained glucuronolactone is greater than 99.2%. The glucuronolactone is prepared by enzymatic conversion of the intermediate inositol, the by-product is high-value potassium dihydrogen phosphate, the whole has good economic benefits, and industrial production can be realized.

The present application relates to the technical field of antibody conjugated drugs, in particular to a drug conjugate with sustained-release property, an antibody conjugated drug and application thereof. The drug conjugate Payload is a payload selected from a drug molecule with cytotoxicity; X is a heteroatom in the payload, selected from O or N; R4 and R5 are independently selected from hydrogen, an alkyl group or other chemical modification groups; R1, R2, R3 and R6 are independently selected from hydrogen, an amino group, a group containing an ether bond, a hydrophilic group, a halogen, an alkyl group or other chemical modification groups; and Glu is a glucuronic acid group. The present application has the advantages of achieving sustained-release effect, increasing stability, avoiding possible non-specific rupture of carbamate bonds in in-vivo circulation, improving the half-life of ADC drugs in blood circulation, reducing toxicity and increasing drug efficacy, and achieving more effective and safe treatment effect due to the sustained-release mechanism which maintains local toxin concentration in a more reasonable killing interval and reduces systemic toxic side effects.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

This invention relates to a method for identifying and detecting urinary biomarkers of whole-grain wheat intake. These biomarkers include glycine conjugates of 3,5-dihydroxybenzoic acid, sulfate conjugates of o-aminophenol, sulfate conjugates of 2-acetaminophen, sulfate conjugates of 3,5-dihydroxyphenylvaleric acid, glucuronic acid conjugates of o-aminophenol, or glucuronic acid conjugates of 2-acetaminophen. The invention also relates to a high-throughput screening method for urinary biomarkers of whole-grain wheat intake. A whole-grain wheat dietary intervention experiment was designed, and through comparative analysis of human urinary metabolic profiles, non-targeted metabolomics technology was used to screen for urinary metabolic biomarkers of whole-grain wheat, providing a method for accurately assessing whole-grain wheat intake.

The invention belongs to the technical field of traditional Chinese medicine, and relates to polygonum cuspidatum homogeneous polysaccharide and a preparation method and application thereof.The polygonum cuspidatum homogeneous polysaccharide is mainly composed of galacturonic acid, rhamnose, galactose, glucuronic acid, glucose, mannose, xylose and glucuronide amine according to the molar ratio of 41.967: 24.624: 17.951: 5.427: 4.59: 3.501: 1.236: 0.358, and the weight-average molecular weight of the polygonum cuspidatum homogeneous polysaccharide is 26.57 kDa. The polygonum cuspidatum homogeneous polysaccharide is fixed in component, high in yield, good in hypoglycemic effect and good in safety, and a basis is provided for development of innovative hypoglycemic drugs.

The present application belongs to the technical field of sugarcane leaf polysaccharide regulating intestinal flora. The present application provides a sugarcane leaf polysaccharide composition, which comprises mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose in a mass ratio of 6.8:1.5:5.2:40.7:13.1:18.9:1.6:6.1:1.9. The composition of the present application can improve the imbalance of intestinal flora caused by obesity and hyperglycemia by reducing the ratio of Firmicutes / Bacteroidetes and increasing the abundance of probiotics.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The invention discloses porphyra yezoensis polysaccharide PSP-I with anti-ultraviolet damage activity as well as a preparation method and application of the porphyra yezoensis polysaccharide PSP-I. The Mw of the PSP-I is 26.149 kDa, the Mn of the PSP-I is 11.267 kDa, and the molar ratio of galactose to glucose to glucuronic acid in monosaccharide composition of the PSP-I is 96.76: 2.31: 0.93. The preparation method comprises the following steps: pretreating a nori raw material, extracting crude polysaccharide by adopting an enzyme method, deproteinizing, dialyzing, collecting a main polysaccharide elution peak by utilizing anion exchange chromatography, further purifying by virtue of sephadex chromatography, collecting the main elution peak, removing impurities and drying, thereby obtaining the nori polysaccharide. The polysaccharide PSP-I can effectively remove active oxygen induced by UVB, inhibit apoptosis and down-regulate expression of matrix metalloproteinase in cells under a safe concentration, shows excellent anti-light aging activity, and can be used for preparing cosmetics or skin care products for resisting ultraviolet damage.

The present application relates to a method for regulating the growth of calcium carbonate crystals into calcite by glucose uronic acid, so as to improve the mechanical strength of the solidified body. The specific steps of the method are as follows: (1) screening and identification of acetate mineralization bacteria with calcium acetate as substrate; (2) culture of carbonate mineralization strain; (3) growth and regulation of calcium carbonate crystals induced by microorganisms; (4) characterization of calcium carbonate crystals. The microbial solidification of the uranium tailings beach is a green solidification method with great application prospect. The carbonate mineralization strain provided by the present application has no secondary pollution in the process of microbial induction of calcium carbonate precipitation, has strong environmental friendliness, and can regulate the growth of calcium carbonate crystals into calcite by glucose uronic acid, so as to improve the mechanical strength of the calcium carbonate crystals, and has potential application prospect.

The invention discloses a stringy stonecrop herb polysaccharide extraction process which comprises the following steps: drying and crushing stringy stonecrop herb, adding water according to a material-liquid ratio of (1: 20)-(1: 60), extracting, concentrating, and carrying out alcohol precipitation to obtain crude polysaccharide; deproteinizing, dialyzing and freeze-drying; carrying out gradient elution and purification to obtain sedum sarmentosum polysaccharide SSBP; an extraction process is optimized through a response surface method, optimal conditions are selected, and the yield of crude polysaccharide reaches 19.06%; according to structural characterization, SSBP is acidic polysaccharide, the molecular weight is 7.484 kDa, 36.12% of uronic acid is contained, monosaccharide is composed of rhamnose, arabinose, galactose, galacturonic acid and glucuronic acid, and the molar ratio of rhamnose to arabinose to galactose to galacturonic acid to glucuronic acid is 12.85: 19.13: 18.98: 35.73: 3.35; an in-vitro activity experiment shows that the SSBP has good antioxidant activity and remarkable anti-inflammatory activity, the release of RAW264.7 macrophage NO induced by LPS and the secretion of IL-6, IL-1beta and TNF-alpha can be inhibited in a dose-dependent manner, and the inhibition rates under the concentration of 1.2 mg / mL respectively reach 74.41%, 92.53%, 95.27% and 95.73%; and a new scheme is provided for deep development of sedum sarmentosum polysaccharide and preparation of anti-inflammatory and anti-oxidation products.

The application discloses a rehmannia polysaccharide, a preparation method thereof and application of the rehmannia polysaccharide in preparation of a medicine for preventing and treating kidney injury caused by cisplatin. The rehmannia polysaccharide contains the following monosaccharides in percentage by mass: rhamnose (Rha) 10.265%-14.963%, glucuronic acid (GlcA) 0.514%-0.904%, galacturonic acid (GalA) 35.784%-40.596%, glucose (Glc) 3.128%-5.048%, galactose (Gal) 37.892%-41.438% and arabinose (Ara) 3.869%-4.865%. The structure of the rehmannia polysaccharide is clear, and the rehmannia polysaccharide shows a significant improvement effect on cisplatin nephrotoxicity through cell experiments and animal experiments, and has important value for developing a new and effective prevention and treatment product of cisplatin-induced acute kidney injury.

The invention relates to beekeeping, in particular to a process for producing royal jelly.The process, according to the invention, includes the introduction into the breeding family of frames equipped with qeen cells with transferred larvae and the daily feeding of the nurse bees, in the absence of a maintenance honey flow, with a mixture of sugar syrup and a biostimulator, for 3 days, after which the frames are removed from the hive to collect the royal jelly, and the procedure is repeated twice, at the same time the biostimulator is an aqueous solution containing: D-glucuronic acid, potassium hydroxide, choline chloride and water.

The invention discloses a method for detecting glucuronic acid in a calcium gluconate bulk drug. The method has high system applicability, has incomparable advantages in specificity, quantitation limit, detection limit, linear range, repeatability and durability, and has high precision.

This invention discloses a method for preparing a highly water-resistant, glue-free, synergistically cross-linked bamboo-based board, comprising the following steps: preparing polyphenol-modified bamboo powder; adding glucuronic acid solution dropwise to the polyphenol-modified bamboo powder, reacting at 50℃~65℃, then adding citric acid solution, and reacting again to obtain cross-linked modified bamboo powder; adjusting the moisture content of the cross-linked modified bamboo powder to 8wt%~12wt%, and pre-pressing it into a blank; covering the upper and lower surfaces of the blank with a release film and a steel plate respectively, then placing it in a flatbed hot press, and performing segmented pressing molding at 155℃~165℃ to obtain the highly water-resistant, glue-free, synergistically cross-linked bamboo-based board. The resulting board requires no traditional adhesives, possesses high mechanical strength, excellent water resistance, and dimensional stability, and the process is mild with green raw materials, making it suitable for the field of green building materials and providing a new approach for the efficient utilization of bamboo resources.

The invention discloses a preparation method of fructus alpiniae oxyphyllae pectic polysaccharide and application of the fructus alpiniae oxyphyllae pectic polysaccharide in treatment of Alzheimer's disease. The pectic polysaccharide is prepared by taking fructus alpiniae oxyphyllae as a raw material through the steps of degreasing, water extraction, alcohol precipitation, deproteinization, dialysis, chromatographic purification and the like, and is a uniform acidic polysaccharide. Structural characterization results show that the pectic polysaccharide is mainly composed of rhamnose, glucuronic acid, galacturonic acid and galactose, the content of galacturonic acid is dominant, part of residues are subjected to O-methylation and / or O-acetylation modification, and the pectic polysaccharide has a pectic acidic polysaccharide structure with alpha-(1-> 4)-D-galacturonic acid as a main chain and branched chains. Animal experiment results show that the fructus alpiniae oxyphyllae pectic polysaccharide can significantly improve the learning and memory ability and cognitive impairment of Alzheimer disease model animals. The fructus alpiniae oxyphyllae pectic polysaccharide provided by the invention is clear in source and stable in preparation method, can be used for preparing a pharmaceutical composition or functional food for preventing and treating the Alzheimer disease, and has a good application prospect.

This invention provides an extract of Astragalus complanatus and its preparation method. The extract comprises the following raw materials in molar percentages: Astragalus complanatus polysaccharide 31.79%–84.74%, protein 6.56%–34.35%, uronic acid 4.07%–27.11%, polyphenols 3.76%–10.83%, rhamnose 0.4%–3.35%, arabinose 0.97%–22.55%, xylose 0.30%–25.28%, mannose 0–58.37%, glucose 0–1.27%, galactose 17.38%–43.03%, and glucuronic acid 0.58%–55.71%. The Astragalus complanatus extract of this invention has the advantages of high purity, clearly defined active ingredients, and uniform stability. In vivo efficacy experiments using zebrafish have shown that the Astragalus complanatus extract of this invention has significant therapeutic effects on fatty liver, non-alcoholic steatohepatitis, alcoholic steatohepatitis, and hyperlipidemia.

The invention belongs to the field of quantitative analysis of drug concentration of a cell lysis solution, and particularly discloses a method for detecting estradiol 17 beta-D glucuronic acid (Estradil 17-(beta-D-Glucuronide)) in the cell lysis solution. According to the method, the concentration of the estradiol 17 beta-D glucuronic acid in the cell lysis buffer can be accurately quantified through specific sample treatment and liquid chromatography and mass spectrometry conditions, the sensitivity is high, the detection limit is low, a novel method capable of being practically popularized and applied is provided for pharmacokinetic research of the estradiol 17 beta-D glucuronic acid, and the method has popularization and application values.

The application relates to the technical field of washing and protecting products, in particular to a traditional Chinese medicine soothing liquid for nourishing the scalp and a preparation method thereof. The traditional Chinese medicine soothing liquid comprises a traditional Chinese medicine extract and an additive. The preparation method of the traditional Chinese medicine soothing liquid is as follows: S1, pulverizing radix polygoni multifidi, biota orientalis and rhizoma alocasia, adding an ethanol solution to heat and reflux extraction to obtain medicinal liquid A; pulverizing herba polygoni avicularis, radix bupleuri, herba cynomorii, semen abrus and semen euryales, adding water to heat and reflux extraction to obtain medicinal liquid B, and combining medicinal liquid A and medicinal liquid B to obtain a traditional Chinese medicine extract; S2, adding an additive to the traditional Chinese medicine extract, stirring and uniformly mixing to prepare the traditional Chinese medicine soothing liquid; the additive comprises fucus vesiculosus extract, galacturonic acid, glucuronic acid, a humectant, a surfactant and a preservative; and the traditional Chinese medicine soothing liquid has the advantages of improving the efficacy of the traditional Chinese medicine composition on the scalp improvement.

This invention relates to an aqueous emulsion comprising: (A) an ester of fatty acids and polyglycerol having 16 to 22 carbon atoms, an ester of fatty acids and glycerol having 16 to 22 carbon atoms, and a monohydric alcohol having 16 to 22 carbon atoms; (B) a compound having at least two hydroxyl groups and an alkyl group having 16 to 22 carbon atoms and having no phosphorus atoms, or a salt thereof, wherein component (B) is different from component (A); and (C) a cellulose thickener and / or a polysaccharide thickener comprising glucose, glucuronic acid, and rhamnose as the main chain of constituent monosaccharides.

This invention relates to the field of biotechnology, specifically to an inositol oxidase mutant and its application in the production of glucuronic acid. The inositol oxidase mutant provided by this invention is obtained by point mutation of the amino acid sequence shown in the wild-type inositol oxidase. Compared with the wild-type enzyme, the inositol oxidase mutant constructed by this invention exhibits a significant improvement in catalytic efficiency. Furthermore, this invention also provides an engineered strain with site-directed mutagenesis and knockout of the UxaC gene in the chassis cell genome. This strain exhibits higher catalytic activity towards the substrate, with a catalytic efficiency increased by 74.44% compared to the wild-type enzyme. Even at high substrate concentrations, the conversion rate can still reach over 92.09%, with a production intensity as high as 13.23 g / L / h. It possesses extremely strong substrate tolerance and production intensity, significantly reducing the production cost of glucuronic acid and demonstrating significant industrial application potential.

Provided are a hyaluronic acid derivative and a hyaluronic acid derivative cross-linked gel which have excellent in vivo swelling inhibition performance after gelling, and a pharmaceutical composition using the hyaluronic acid derivative and the hyaluronic acid derivative cross-linked gel. The present invention relates to: a hyaluronic acid derivative in which a steroid group is introduced to at least some of the carboxyl groups in the glucuronic acid moiety of hyaluronic acid, and a maleimide group is introduced to at least some of the hydroxyl groups in the N-acetylglucosamine moiety or the carboxyl groups in the glucuronic acid moiety of hyaluronic acid; and a hyaluronic acid derivative cross-linked gel which is a gel-like substance in which the hyaluronic acid derivative is chemically cross-linked by a cross-linking agent having two or more cross-linkable groups.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

The invention relates to a preparation method of hericium erinaceus polysaccharide, in particular to a preparation method for improving the yield of hericium erinaceus polysaccharide and a corresponding anti-inflammatory product thereof. The invention firstly provides a preparation method for improving the yield of the hericium erinaceus polysaccharide, it is surprisingly found that the combination of different carbon sources and nitrogen sources and the mass ratio range have remarkable influences on the yield of the hericium erinaceus polysaccharide, and a fermentation culture solution with a synergistic effect is preferably obtained through tests; the carbon source is composed of glucose, maltose and galactose in a mass ratio of 1: 1: 4; a nitrogen source is composed of peptone and ammonium nitrate, and the mass ratio of peptone to ammonium nitrate is 1: 2-1: 4. The invention further provides refined hericium erinaceus polysaccharide with efficient anti-inflammatory activity, the structure of the refined hericium erinaceus polysaccharide is composed of glucose (Glc), galactose (Gal), glucuronic acid (Glc-UA), fucose (Fuc) and ribose (Rib) according to the mass percentage ratio of 38.84: 46.87: 7.59: 3.54: 3.16, and the hericium erinaceus polysaccharide has important significance in the aspect of preparing anti-inflammatory drugs.