174 results about "D-GLUCURONIC ACID" patented technology

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention discloses arabinose glycosyl transferase PnUGT73B4 and application thereof to preparation of panax japonicus saponin IV, the amino acid sequence of the arabinose glycosyl transferase is shown as SEQ ID NO: 1, under the catalysis of the arabinose glycosyl transferase PnUGT73B4, UDP-arabinose is added to a glucuronic acid group on the C3 site of panax japonicus saponin IVa, and the panax japonicus saponin IV is generated; according to the method, the requirement for raw material plants can be reduced, agricultural land is saved, the product is single, preparation of the panax japonicus saponin IV is facilitated, the biosynthesis route of oleanane type triterpenoid saponin is analyzed, and a breakthrough solution is provided for biosynthesis of the panax japonicus saponin IV.

The invention relates to the field of microbial strains edited by gene recombination and synthetic biology, and provides recombinant escherichia coli for synthesizing D-xylose by using glucose and application. The recombinant escherichia coli comprises the following modifications: exogenous insertion of a gene IRX for coding beta-1, 4-xylosyltransferase; exogenous insertion of a gene UXS encoding a UDP-glucuronic acid decarboxylase; the invention relates to enhanced expression or overexpression of a gene yagH for coding beta xylosidase. Compared with a traditional hemicellulose hydrolysis method for producing D-xylose, the method for synthesizing and preparing D-xylose through whole-cell catalysis of D-glucose by using the recombinant escherichia coli has the advantages of environmental protection, cost advantage and energy saving.

The invention relates to the technical field of biology, in particular to a recombinant strain for producing glucuronic acid and application of the recombinant strain. The invention provides a recombinant strain for producing glucuronic acid, and the recombinant strain does not express uronic acid isomerase and overexpresses inositol oxidase. The technical scheme of the invention has the following advantages: (1) uronic acid isomerase uxaC from escherichia coli K12 is knocked out, so that glucuronic acid accumulation is increased; and (2) successfully integrating the target gene of the inositol oxidase from the mouse into the escherichia coli K12MG1655. And (3) when the engineering strain is used for catalyzing inositol, the content of glucuronic acid in a system after the reaction is finished is 52.50 g / L, and the conversion rate is as high as 97.23%.

The invention provides application of pitaya peel polysaccharide in preparation of a medicine for repairing skin wounds. The monosaccharide components of the pitaya peel polysaccharide comprise rhamnose, arabinose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. Research finds that the dragon fruit peel polysaccharide remarkably accelerates the wound healing process and promotes granulation tissue formation and collagen deposition. Meanwhile, the pitaya peel polysaccharide can also promote cell proliferation and neovascularization at the skin wound part, exert an anti-inflammatory effect, remodel skin microbiota and provide a more suitable microenvironment for skin wound healing. The invention provides a new drug choice for clinically repairing skin wounds, and can be used for developing new skin wound dressings.

The invention provides recombinant escherichia coli for synthesizing glucuronic acid by using glucose and a method for producing glucuronic acid by using the recombinant escherichia coli, and belongs to the field of microbial metabolic engineering. The engineering bacterium is obtained by taking escherichia coli as an original strain and knocking out a glucuronic acid isomerase coding gene uxaC, a 6-phosphofructokinase I coding gene pfkA, a 6-phosphofructokinase II coding gene pfkB and a glucose-6-phosphate dehydrogenase coding gene zwf. The engineering bacterium disclosed by the invention has a good application prospect in industrial production of glucuronic acid.

The present invention provides a homogenized pumpkin polysaccharide and its preparation method and application, belonging to the technical field of polysaccharide extraction and purification. The homogenized pumpkin polysaccharide of the present invention contains mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, with the corresponding molar ratios being 0.005:0.004:0.041:0.013:0.545:0.004:0.241:0.147; the relative molecular weight of the homogenized pumpkin polysaccharide is 5.96×10 4 The homogenized pumpkin polysaccharide has significant lipid-lowering and blood sugar-lowering effects. The preparation method has mild process conditions and simple operation. The obtained polysaccharide has high purity and stable structure, and is suitable for industrial-scale production.

The invention discloses application of GUX1 protein and related biological materials thereof in regulation and control of xylan glucuronic acid modification. Through overexpression of GUX1 in rice and tobacco, the invention finds that the short interval substitution of xylan glucuronic acid in an acceptor material is specifically increased. According to the invention, the function of short-interval glucuronic acid addition of GUX1 mediated xylan is found for the first time, and the GUX1 gene can be used for regulating a glucuronic acid modification mode of plant xylan and producing a corresponding xylan structure product, and is an important and valuable gene for cell wall structure and function modification.

This invention belongs to the field of photocrosslinking probe technology, specifically relating to a GUSB photocrosslinking probe, its preparation method, and its application. The GUSB photocrosslinking probe has the general chemical formula C0. 13 H 18 N2O6-R, wherein R is any one of the oxalic group elements or peptide bonds. The R group covalently connects the glucuronic acid recognition core and the photosensitive unit in the GUSB photocrosslinking probe, and the R group contains no more than 4 atoms, resulting in a small size and low steric hindrance. The GUSB photocrosslinking probe provided by this invention uses glucuronic acid as the recognition core, combined with a small-volume diazacyclopropane photosensitive unit and a terminal alkyne group, to achieve µM-level in-situ covalent capture of active GUSB in complex systems such as cells or tissues. The probe of this invention significantly improves positioning accuracy, sensitivity, data availability, quantification, and enrichment, while also possessing advantages such as modular synthesis, mild conditions, and strong system compatibility, effectively addressing the technical shortcomings of existing technologies that struggle to accurately obtain GUSB activity in situ under low-dose conditions.

The present application relates to the technical field of medicine, and provides a kind of haena polysaccharide and its preparation method and application and target endogenous coagulation pathway anticoagulant and / or antithrombotic drug.The haena polysaccharide provided by the present application is fucosylated chondroitin sulfate polysaccharide, the weight average molecular weight is 9-13 million, the molar ratio of glucuronic acid, N-acetylglucosamine and fucose is 1:0.8-1.2:0.5-0.8, and the mass percentage content of sulfate group is 25-40%.The haena polysaccharide provided by the present application can target endogenous coagulation pathway terminal rate-limiting enzyme (iFXase), and has no obvious effect on coagulation factors in other coagulation pathways, realizes the effect of anticoagulation and non-hemorrhage, and can be widely applied in acute and recovery period treatment of ischemic stroke, solves the safety problem of using anticoagulant and antithrombotic drug for clinical ischemic stroke patients, and opens up a new field of research and development of target endogenous coagulation pathway rate-limiting enzyme anticoagulant drug.

The present invention discloses a Sepia esculenta ink polysaccharide for improving high-glucose damage of endothelial cells, and a preparation method and application thereof. The Sepia esculenta ink polysaccharide has a polysaccharide backbone composed of fucose, galactosamine, mannose, and N-acetylglucosamine, and has a glucuronic acid branch chain at the C-3 position of mannose. It is an aminopolysaccharide with a weight-average molecular weight of 10-16 kDa and a sulfation degree of 8%-15%. Through experimental verification, compared with similar compounds, the Sepia esculenta ink polysaccharide can effectively improve the state of endothelial cells and maintain the normal filtration function of vascular endothelium. At the same time, the risk of bleeding side effects of the Sepia esculenta ink polysaccharide is significantly lower than that of similar polysaccharides, and it has higher safety in use. Furthermore, the Sepia esculenta ink polysaccharide can be used to develop adjuvant therapeutic drugs for late-stage diabetic lesions, filling the gap in such products on the current market.

The invention belongs to the technical field of biological medicine, and particularly relates to wheat bran araboxylan and application thereof. The invention provides wheat bran AX with specific molecular weight and monosaccharide composition. The wheat bran AX comprises the following monosaccharides: 0.20%-0.22% of glucuronic acid, 0.58%-0.62% of galacturonic acid, 0.21%-0.23% of rhamnose, 0.6%-0.7% of glucose, 1.4%-1.5% of galactose, 18%-19.2% of xylose and 21%-23% of arabinose. Compared with commercially available wheat bran AX, inulin, fructo-oligosaccharide and other common dietary fibers, the self-made wheat bran AX has higher diversity of microbial communities in the fermentation process, has a better intestinal flora regulating effect, further has a good relieving effect on the abnormal glucose and lipid metabolism of the body, and can be used for preventing and treating the abnormal glucose and lipid metabolism of the body. Therefore, the traditional Chinese medicine composition has a good regulation effect on various diseases such as non-alcoholic fatty liver and the like caused by abnormal glucose and lipid metabolism of the organism.

The invention relates to the technical field of pet oral health care, in particular to a compound seaweed source composition for assisting in improving oral health of dogs and cats and application of the compound seaweed source composition. The invention discloses a compound seaweed source composition for assisting in improving oral health of dogs and cats. The compound seaweed source composition comprises sodium alginate and fucoidan, the value of mannuronic acid (M) / guluronic acid (G) in the sodium alginate is 0.96-1.18; the fucoidan is prepared from mannose, glucuronic acid, galactose, xylose and fucose; the weight ratio of the sodium alginate to the fucoidan is 9: 2. The compound seaweed source composition disclosed by the invention has the effects of inhibiting the proliferation of pathogenic bacteria in the oral cavity of a pet, slowing down the rising rate of health indexes of the oral cavity of the pet, inhibiting the generation of volatile organic compounds and ammonia gas in the oral cavity, improving saliva components, slowing down the generation of dental plaque and the like, and can effectively improve the health condition of the oral cavity of the pet.

The application discloses a glucuronolactone and a preparation method thereof, and belongs to the technical field of fine chemical product preparation. The method takes potassium phytate extracted from corn soaking liquid or rice bran acid soaking liquid as raw material, obtains inositol and potassium dihydrogen phosphate after hydrolysis, separates the hydrolysis liquid through chromatography, obtains potassium dihydrogen phosphate by concentrating and crystallizing the salt phase, and the material phase is inositol solution. Enzymatic conversion reaction is carried out by using fermentation to obtain enzyme liquid, glucuronic acid liquid is obtained, glucuronolactone is obtained by concentrating and esterifying, and then the glucuronolactone is obtained by concentrating, refining and crystallizing. In the method, the yield of potassium phytate to inositol is greater than 95%, the molar conversion rate of inositol enzymatic conversion to glucuronic acid is greater than 95%, and the total yield is greater than 89%, which is much higher than the traditional yield, and the content of the obtained glucuronolactone is greater than 99.2%. The glucuronolactone is prepared by enzymatic conversion of the intermediate inositol, the by-product is high-value potassium dihydrogen phosphate, the whole has good economic benefits, and industrial production can be realized.

The present application relates to the technical field of antibody conjugated drugs, in particular to a drug conjugate with sustained-release property, an antibody conjugated drug and application thereof. The drug conjugate Payload is a payload selected from a drug molecule with cytotoxicity; X is a heteroatom in the payload, selected from O or N; R4 and R5 are independently selected from hydrogen, an alkyl group or other chemical modification groups; R1, R2, R3 and R6 are independently selected from hydrogen, an amino group, a group containing an ether bond, a hydrophilic group, a halogen, an alkyl group or other chemical modification groups; and Glu is a glucuronic acid group. The present application has the advantages of achieving sustained-release effect, increasing stability, avoiding possible non-specific rupture of carbamate bonds in in-vivo circulation, improving the half-life of ADC drugs in blood circulation, reducing toxicity and increasing drug efficacy, and achieving more effective and safe treatment effect due to the sustained-release mechanism which maintains local toxin concentration in a more reasonable killing interval and reduces systemic toxic side effects.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

This invention relates to a method for identifying and detecting urinary biomarkers of whole-grain wheat intake. These biomarkers include glycine conjugates of 3,5-dihydroxybenzoic acid, sulfate conjugates of o-aminophenol, sulfate conjugates of 2-acetaminophen, sulfate conjugates of 3,5-dihydroxyphenylvaleric acid, glucuronic acid conjugates of o-aminophenol, or glucuronic acid conjugates of 2-acetaminophen. The invention also relates to a high-throughput screening method for urinary biomarkers of whole-grain wheat intake. A whole-grain wheat dietary intervention experiment was designed, and through comparative analysis of human urinary metabolic profiles, non-targeted metabolomics technology was used to screen for urinary metabolic biomarkers of whole-grain wheat, providing a method for accurately assessing whole-grain wheat intake.

The invention belongs to the technical field of traditional Chinese medicine, and relates to polygonum cuspidatum homogeneous polysaccharide and a preparation method and application thereof.The polygonum cuspidatum homogeneous polysaccharide is mainly composed of galacturonic acid, rhamnose, galactose, glucuronic acid, glucose, mannose, xylose and glucuronide amine according to the molar ratio of 41.967: 24.624: 17.951: 5.427: 4.59: 3.501: 1.236: 0.358, and the weight-average molecular weight of the polygonum cuspidatum homogeneous polysaccharide is 26.57 kDa. The polygonum cuspidatum homogeneous polysaccharide is fixed in component, high in yield, good in hypoglycemic effect and good in safety, and a basis is provided for development of innovative hypoglycemic drugs.

The invention belongs to the technical field of medicines, and particularly relates to safflower polysaccharide for lung targeting as well as a preparation method and application thereof. The safflower polysaccharide comprises the following monosaccharides in percentage by mole: 0.75% of L-fucose, 36.04% of arabinose, 25.03% of rhamnose, 17.85% of galactose, 4.26% of glucose, 1.33% of xylose, 0.61% of mannose, 12.07% of galacturonic acid and 2.06% of glucuronic acid, and the total percentage is 100%. The molecular weight of the safflower polysaccharide is 16.27 kDa. The safflower polysaccharide disclosed by the invention can be absorbed into blood within 24 hours and almost completely metabolized, and can be used for preparing lung targeting products.

The present invention belongs to the technical field of health care beverages, and particularly relates to a preparation method and application of distiller's grains polysaccharide with beneficial biological activities. The distiller's grains polysaccharide is composed of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose; and its molar ratio is: 3.280:0.229:0.268:1.365:0.783:81.879:3.284:4.104:4.687:0.120. The preparation method comprises the following steps: (1) drying the distiller's grains, crushing and sieving them to obtain distiller's grains powder; (2) mixing the distiller's grains powder obtained in step (1) with distilled water in a certain proportion, performing water bath extraction, centrifuging, taking the supernatant and concentrating it, and then performing rotary evaporation to obtain a polysaccharide extract; (3) performing alcohol precipitation, deproteinization, dialysis and freeze-drying on the polysaccharide extract obtained in step (2), and then the distiller's grains polysaccharide is obtained. The distiller's grains polysaccharide with beneficial biological activities prepared by the present invention can regulate the intestinal flora and has the health care and nourishing effects.

This invention discloses the application of Ganoderma lucidum strain SS30 in the preparation of drugs for treating cancer, belonging to the field of biotechnology. This invention provides a polysaccharide from the mycelium of Ganoderma lucidum strain SS30, composed of glucose, galactose, mannose, fucose, xylose, arabinose, rhamnose, glucuronic acid, glucosamine, ribose, and lacturonic acid. This polysaccharide exhibits significant inhibitory effects on transplanted pancreatic cancer and primary colorectal cancer, and can achieve its cancer-treating effect by enhancing immune function and reducing the level of oxidative products. It provides new materials for the development and utilization of Ganoderma lucidum and its products, and lays a research foundation for the development of cancer-related drugs and the study of cancer treatment mechanisms.

The application provides a pueraria ferment as well as a preparation method and application thereof. The preparation method of the plant ferment comprises the following steps: step 1, crushing dry pueraria root powder, mixing with water to obtain a pueraria root powder mixture; step 2, mixing the pueraria root powder mixture with a compound enzyme preparation to perform enzymolysis, to obtain an enzymolysis liquid; step 3, adding a carbon source into the enzymolysis liquid, mixing, and then performing a two-stage fermentation process; the first-stage fermentation is aerobic fermentation by using a bacterial agent containing yeast and acetic acid bacteria, and the second-stage fermentation is anaerobic fermentation by using a bacterial agent containing lactobacillus; and step 4, filtering the fermented mixture to obtain a clear solution, and the pueraria ferment is obtained. The obtained pueraria ferment has high contents of active ingredients such as puerarin and glucuronic acid, has good antioxidant capacity, has a health care effect, and has a liver protection effect.

The present application belongs to the technical field of sugarcane leaf polysaccharide regulating intestinal flora. The present application provides a sugarcane leaf polysaccharide composition, which comprises mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose in a mass ratio of 6.8:1.5:5.2:40.7:13.1:18.9:1.6:6.1:1.9. The composition of the present application can improve the imbalance of intestinal flora caused by obesity and hyperglycemia by reducing the ratio of Firmicutes / Bacteroidetes and increasing the abundance of probiotics.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The invention relates to a uridine diphosphate-glucose dehydrogenase mutant and an application of the uridine diphosphate-glucose dehydrogenase mutant in synthesis of uridine diphosphate-glucuronic acid. The amino acid sequence of the uridine diphosphate-glucose dehydrogenase mutant D7P166M / I169A is as shown in SEQ ID NO. 2, and the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides an application of the mutant in preparation of uridine diphosphate-glucuronic acid, and a method for preparing uridine diphosphate-glucuronic acid by using the mutant. Experimental determination shows that the melting temperature of the mutant is increased by 30 DEG C compared with that of a wild type, the activity is improved by 20%, and a powerful tool is provided for enzymatic synthesis of uridine diphosphate-glucuronic acid compounds. Compared with a conventional synthesis pathway, the method for synthesizing uridine diphosphate-glucuronic acid through immobilized enzyme flow provided by the invention can be used for stably and efficiently synthesizing for a long time, and is beneficial to reducing the cost and realizing industrial synthesis.

The invention provides a preparation method of a scaffold for promoting collagenous fiber assembly and internal and external mineralization. The preparation method comprises the following steps: A) adding glycine and potassium chloride into a compound solution formed by chelating non-collagen analogues with calcium ions to prepare an assembly solution; wherein the non-collagen analogue is one or two of polycarboxylic acid substances and glucuronic acid; b) adding a collagen solution into the assembly solution obtained in the step A) to obtain a mixed solution, and adjusting the pH value of the mixed solution to 7.0 + / -0.2; placing the mixed solution in an incubator at 37 + / -0.5 DEG C, incubating for 12-24 hours, and assembling collagen to obtain collagen fibers; c) placing the collagenous fibers prepared in the step B) in a collagenous fiber mineralizing solution for mineralization to obtain mineralized collagenous fibers, and washing, freezing and drying the mineralized collagenous fibers to obtain a mineralized collagenous fiber scaffold; according to the invention, the bone tissue generation process is simulated in a safer bionic manner, so that the collagenous fiber scaffold is assembled and mineralized more efficiently.

Disclosed is a chemical synthesis method for Pseudomonas aeruginosa O5 serotype O-antigen oligosaccharide, which belongs to the field of chemical synthesis. In the disclosure, O-antigen trisaccharide is constructed with a D-glucuronic acid building block and a D-fucosamine building block, where the stereoselective synthesis of a 1,2-α-cis-glycosidic bond of D-fucosamine depends on remote acyl participation and reagent regulation, and synthesis of two types of 1,2-β-trans-glycosidic bonds of 2,3-diaminomannuronic acids is achieved via SN2 nucleophilic substitution of azido at position C2; and via selective assembly of protecting groups, orthogonal modification of modifying groups, and regulation of the reactivity of glycosyl donors and acceptors, multifunctional modified O-antigen target trisaccharide is successfully prepared. According to the method in the disclosure, the raw materials are cheap and easily available, and the preparation method is simple and easy to repeat. Therefore, the method has a very good application prospect in developing vaccines against P. aeruginosa.