31 results about "Phanerochaete sp." patented technology

The invention discloses an immobilized biological adsorbent containing phanerochete chrysosporium, the surface of which is uniformly wrapped by a layer of carrier Fe3O4 nanoparticles for fixation. The method comprises the steps of: first adding Fe3O4 nanoparticles into a phanerochete chrysosporium liquid medium containing tween 80, then inoculating a phanerochete chrysosporium spore suspension, conducting shaking cultivation so as to obtain the immobilized biological adsorbent. The invention also discloses application of the adsorbent in dye wastewater treatment. The biological adsorbent of the invention has high adsorption efficiency and large adsorption capacity, and can be effectively used for treating neutral red and lead in dye wastewater. The preparation technology and treatment technology in the invention are characterized by simplicity and low cost.

The invention discloses a method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement. Contaminated soil or oil sludge is oxidized first with a sodium persulfate compound as the oxidizing agent in the presence of a surfactant, and then a mixed flora of bacillus sp. and acremonium sp. Y0997 or phanerochaete sp. F0996 is added twice continuously, so that intensified combined treatment of soil or oil sludge is achieved through intensified combination of chemical oxidization and fungus-bacterium flora biological sequences. In this way, the degrading efficiency of soil or oil sludge severely contaminated by petroleum can be improved remarkably, and repair period can be shortened greatly.

The invention discloses a method for removing a heavy metal pollutant from a water body by using phanerochete chrysosporium. The method comprises the following steps of: preparing spore suspension by using phanerochete chrysosporium spore powder; inoculating into a liquid culture medium for shake culture; filtering to obtain phanerochete chrysosporium pellets; adding the pellets into wastewater, adjusting the pH value of the wastewater, performing adsorption reaction under a constant temperature condition, filtering the wastewater after the reaction, and recovering the pellets to finish a removal process one time; cleaning the recovered pellets by using deionized water and then adding the cleaned pellets into culture solution for continuous culture so as to obtain phanerochete chrysosporium regenerative pellets; and adding the phanerochete chrysosporium regenerative pellets into heavy metal wastewater to be treated for cycling. By the method, hyphae are not required to be modified and pretreated and can be repeatedly used, the reaction condition is simple, and the water body of a complex composition can be treated.

The invention relates to a cultural method for improving degradation performance of phanerochaete chrysosporium, and belongs to the bioengineering field. Silicon substrate nanometer particles are added to a phanerochaete chrysosporium fluid medium to conduct fermental cultivation, due to functions among the silicon substrate nanometer particles and spore and mucelium, and meanwhile the silicon substrate nanometer particles enter the interior of mycelium pellets, and a spherical phanerochaete chrysosporium group which has excellent application effect in dye degradation aspect is acquired.

The invention discloses a structure capable of being used for treating heavy metals in bottom mud dredging tail water. The structure comprises a settling pond and a reaction pool, wherein the reaction pool comprises an adjusting column and at least one absorption column. The adjusting column and the absorption columns use a grating cylinder mould as a frame, the adjusting column is filled with a solid particle filler and the absorption columns are filled with compounds consisting of Fe3O4-calcium alginate-phanerochaete chrysosporium microspheres. The adjusting column and the absorption columns are sequentially arranged along a water flow direction. A construction method of the structure comprises the following steps: constructing the settling pond and the reaction pool; digging more than two grooves in the reaction pool; placing the grating cylinder mould in a direction vertical to the water follow; filling the solid particle filler to the most upstream groove, filling compound biological absorption materials in the balance of grooves, and ensuring that the filler is higher than the water line of the reaction pool. The method disclosed by the invention has the advantages of low cost, simple preparation process, high absorption efficiency and the like, and can be used for treating heavy metal pollutants in the bottom mud dredging tail water.

The invention discloses a high-efficiency aerobic composting inoculant for livestock and poultry manure and a method for aerobic composting thereof. The inoculant is composed of the following bacterial species by following volume proportion: the ratio of Bacillus licheniformis: Phanerochaete chrysosporium: Aspergillus niger is 1:4 to 5:5-6. The aerobic composting comprises the following steps: S1.adding agricultural and forestry waste to the manure of livestock and poultry, adjusting the C/N of livestock manure to 28-30 and the water content being 55%-60%; and S2. adding a sugar source to thelivestock and poultry manure regulated in S1, then adding the above-mentioned inoculant, and uniformly mixing the materials and performing the aerobic composting. After aerobic composting, the degradation of antibiotics in livestock manure is significant, and the content of available heavy metals and resistance genes in livestock manure is greatly reduced, and nitrogen loss is effectively controlled, so that the inoculant has great application prospects.

The invention discloses a compound microbial agent for river regulation, a preparation method of the compound microbial agent and a river sewage treatment method. In the compound microbial agent, a microbial agent A is made by mixing of five fermentation liquors formed by single culture of bacillus megaterium CCTCC NO.M2012352, bacillus subtillis CGMCC NO.1.2162, bacillus licheniformis CGMCC NO.1.6510, bacillus amyloliquefaciens CGMCC NO.1.7463 and phanerochaete chrysosporium BKM-F1767; a microbial agent B is made by mixing of two fermentation liquors formed by single culture of debaryomyces hansenii CGMCC NO.5770 and saccharomyces cerevisiae CGMCC NO.3003. The compound microbial agent has advantages that amino nitrogen and nitro nitrogen in water can be degraded, phosphorus concentration is decreased, and precipitation of solid suspended particles, bacterial cells and colloid particles in water is realized.

The invention discloses a technology for degrading anthraquinone compounds in wastewater by use of phanerochaete chrysosporium, and relates to the field of bioengineering. The phanerochaete chrysosporium is used as an original strain; after liquid shake-flask culture and liquid strain enlarge culture, the bacterial liquid is transferred to the wastewater containing anthraquinone compounds, and certain nutrient substances are supplemented to perform fermentation culture. By adopting the technology, the removal rate of anthraquinone compounds can reach 60-100%.

The invention relates to the technical field of microorganisms, in particular to a complex microbial inoculant for efficient degradation of straw and a preparation method and application of the complex microbial inoculant. The complex microbial inoculant comprises a flora A, a flora B and a flora C; the flora A comprises aspergillus fumigatus, aspergillus versicolor, trichoderma harzianum, phanerochaete chrysosporium and auricularia polytricha; the flora B comprises bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens; and the flora C comprises depubyomyces hansenii, pichia guilliermondii, bacillus subtilis, bacillus cereus and aneurinibacillus. The flora A is used for preparing a microbial agent I, the flora A and the flora B are used for preparing a microbial agent II, the flora A and the flora C are used for preparing a microbial agent III, and the flora A, the flora B and the flora C are used for preparing a microbial agent IV. According to the complex microbial inoculant, the flora A which is close to the environment and has a lignocellulose degradation function is preferably selected from the natural environment by a traditional microbiological method, and the flora B and the flora C are combined to achieve a synergistic effect, so that the straw degradation efficiency is effectively improved, and the complex microbial inoculant has important guiding significance on straw resource utilization.

The invention discloses a multi-effect soil conditioner for improving land capability decline of olive timbered soil by using olive wastes. The multi-effect soil conditioner is prepared from the following raw materials in parts by weight: olive pomace, olive leaves, olive branch pyroligneous, phenolic resin, polymethyl methacrylate, oyster shell powder, polyvinyl alcohol, a biological nutrient, polyacrylamide, bacillus marinus, bacillus thuringiensis, phanerochaete chrysosporium, rape seed cakes, modified charcoal, polyvinyl acetate, maize straws, propionyl brassinolide and ammonium sulfate. The biological nutrient is prepared from yeast extract, cane sugar, peptone, potassium acetate and sodium phosphate in a ratio of 4: 3: 2: 1: 1. Wastes such as the olive pomace and olive leaves are fully used, soil is improved by a double regulating mode biologically and chemically by using a mixed material of biological, organic, inorganic and high molecular materials, the land capability declineof olive timbered soil is improved, the productivity of the olive timbered soil is improved, and the output of olive is improved.

The invention discloses a method for improving the activity of lignin-degrading enzyme generated by phanerochaete chrysosporium after enzyme activity decay. The method comprises the following steps: fermenting the phanerochaete chrysosporium in a carbon-limiting culture medium, and adding glucose to a fermentation system when a carbon source is used up, so that the activity of the lignin-degrading enzyme generated by the phanerochaete chrysosporium is improved after decay. According to the method, the recovery of the lignin-degrading enzyme activity can be realized only by adding an appropriate amount of glucose to the culturing system, the conditions are easy to control, and the method is suitable for the stable production and practical degradation of the lignin-degrading enzyme generated by phanerochaete chrysosporium.

The invention provides a preparation method of a composite microbial preparation for degrading high-oil-content kitchen waste. The composite microbial preparation comprises acinetobacter johnsonii, candida utilis, phanerochaete chrysosporium, bacillus subtilis and geobacillus stearothermophilus. The method comprises the following steps of S1, inoculating strains subjected to activation culture to a fermentation culture medium according to the mass percent of 5%, and performing fermentation culture, and S2, mixing the thalli cultured in the step S1 to prepare liquid mixed bacteria, mixing the liquid mixed bacteria with a solid adsorption carrier according to a mass ratio of 1: (1.5-2), and carrying out carrier adsorption and drying to obtain a composite microbial preparation. The liquid mixed bacteria comprise 30 to 40 percent of acinetobacter johnsonii, 10 to 20 percent of candida utilis, 10 to 15 percent of phanerochaete chrysosporium, 10 to 20 percent of bacillus subtilis and 10 to 20 percent of geobacillus stearothermophilus. Macromolecular compounds such as animal fat, vegetable fat and cellulose which are not easy to decompose in the kitchen waste can be efficiently and rapidly decomposed, and the degradation rate of protein and starch is maintained.

The invention discloses a high-conversion-rate production method of fermented citric acid. The production method comprises the following steps of mixing wheat flour, wheat straw and wheat bran, performing pulverizing, screening with a 80-mesh sieve, adding water, carrying out ultrasonic soaking at 40-50 DEG C for 5-12 hours at the ultrasonic power of 300-400W, regulating the pH value of the systemto 6.6-6.8, and adding alpha-amylase and cellulase to carry out enzymolysis for 10-20 hours to obtain pretreated wheat flour; filtering and decoloring the pretreated wheat flour to obtain a filtrate,adjusting the total sugar concentration in the filtrate to 12-16%, then adding ammonium sulfate, monopotassium phosphate, vitamin B1 and compound trace elements, and uniformly mixing to obtain a fermentation culture solution; inoculating phanerochaete chrysosporium, trichoderma reesia and aspergillus niger into the fermentation culture solution, introducing air for fermentation in a stirring state with the air introducing amount of 0.4-0.5VVM, performing fermenting at the temperature of 30-40 DEG C until the concentration of residual reducing sugar is less than or equal to 0.2 percent, carrying out filtering, and performing chromatographic separation and extraction.

The invention discloses a method for quickly treating solid waste by using a biological agent, which comprises the following steps: weighing the following microbial agents in parts by weight: bacillussubtilis, geobacillus stearothermophilus, Kocuria palustris, aspergillus niger, rhizopus oryzae, white rot fungi, phanerochaete chrysosporium, lactobacillus acidophilus and saccharomyces cerevisiae,and carrying out slant activation on each microbial agent; inoculating a microbial culture medium with the mixed microbial inoculum for culturing to obtain a complex microbial inoculum; adding cellulase and lignin peroxidase into the complex microbial inoculant, and uniformly mixing to obtain a biological inoculant for solid waste treatment; and directly adding the biological agent into the solidwaste, and carrying out aerobic fermentation. According to the method, through reasonable compatibility of multiple strains and selection of a proper microbial culture medium, organic matters, cellulose and macromolecular refractory substances in the sludge can be rapidly degraded, meanwhile, ammonia nitrogen, COD, heavy metal ions and the like in the sludge can be removed, and pollution of solidwaste to the environment is relieved.

The invention discloses a compound microbial agent for preventing and treating diseases and application thereof, and relates to the technical field of microbial agents. The compound microbial agent comprises the following components: bacillus subtilis, bacillus licheniformis, rhodobacillus palustris, bacillus mucilaginosus, bifidobacterium, phanerochaete chrysosporium, lactic acid bacteria, streptomyces jingyangensis, a stabilizer, chitosan modified bentonite, trace elements and potassium fulvate. The prepared microbial agent in the invention can effectively prevent and treat plant root diseases, has a very prominent prevention and treatment effect, and can be widely applied to agricultural production; and a bio-organic fertilizer prepared by using the complex microbial agent can condition soil, activate the activity rate of microorganisms in the soil, overcome soil hardening and increase the air permeability of the soil, has a prevention and treatment effect on various fungal diseases and bacterial diseases, and has an inhibition effect on viral diseases.

The invention relates to a phanerochaete chrysosporium mycelium growthform controlling method and belongs to the field of bioengineering. The phanerochaete chrysosporium mycelium growthform controlling method comprises the following steps: adding inorganic nanoparticles to a fluid nutrient medium of the phanerochaete chrysosporium to carry out ferment cultivation, and obtaining a mycelium group of the phanerochaete chrysosporium due to the facts that the inorganic nanoparticles react with spores and mycelia, and the inorganic nanoparticles enter into mycelial beads. The mycelium group has a good application effect in dye decolorization.

The invention relates to the technical field of ornamental plant fertilizers, in particular to a scindapsus aureus fertilizer and a preparation method thereof. The scindapsus aureus fertilizer is prepared from the following raw materials in parts by mass: 55 to 78 parts of chemical fertilizer, 10 to 23 parts of microbial agent, 5 to 8 parts of jasmine flower essential oil and 320 to 350 parts of water. The microbial agent is prepared from bacillus subtilis, bacillus licheniformis, active dry yeast, pseudomonas thermophilus, bacillus schlegeli, phanerochaete chrysosporium, paracoccus denitrificans, candida utilis and rhodococcus erythropolis. The added jasmine flower essential oil has light fragrance and better ornamental value; the epipremnum aureum fertilizer can effectively promote rooting and germination of epipremnum aureum at low temperature in winter.