31 results about "Phanerochaete sp." patented technology

The invention discloses a method for treating soil or oil sludge severely contaminated by petroleum based on combination of sodium persulfate compound oxidation and microbial flora biological enhancement. Contaminated soil or oil sludge is oxidized first with a sodium persulfate compound as the oxidizing agent in the presence of a surfactant, and then a mixed flora of bacillus sp. and acremonium sp. Y0997 or phanerochaete sp. F0996 is added twice continuously, so that intensified combined treatment of soil or oil sludge is achieved through intensified combination of chemical oxidization and fungus-bacterium flora biological sequences. In this way, the degrading efficiency of soil or oil sludge severely contaminated by petroleum can be improved remarkably, and repair period can be shortened greatly.

The invention relates to a cultivation method for improving the degradation performance of Phanerochaete chrysosporium, belonging to the field of bioengineering. In the present invention, the silicon-based nanoparticles are added to the Phanerochaete chrysosporium liquid medium for fermentation and cultivation, and through the interaction between the silicon-based nanoparticles, spores and mycelia, the nanoparticles enter the interior of the mycelium ball to obtain spherical yellow Phanerochaete sporogenes mycelial group, the mycelial group has a better application effect in dye degradation.

The invention discloses a structure that can be used for treating heavy metal pollutants in tail water of sediment dredging, including a sedimentation tank and a reaction tank, the reaction tank includes an adjustment column and at least one adsorption column, and the adjustment column and the adsorption column both adopt grids The mesh cage is used as a frame, and the regulating column is filled with solid particle filler, and the adsorption column is filled with a composite composed of Fe3O4-calcium alginate-Phanerochaete chrysosporium microspheres; and the regulating column and the adsorption column are arranged along the direction of water flow in sequence. The construction of the structure includes the following steps: build the sedimentation tank and the reaction tank, excavate more than two grooves in the reaction tank, and place the grid cage in each groove vertically to the direction of water flow; Fill the solid particle filler in the groove, and fill the composite bioabsorbent material in the remaining grooves, and ensure that the filler is higher than the water level line of the reaction tank. The invention has the advantages of low cost, simple preparation process, high adsorption efficiency, etc., and can be used for treating heavy metal pollutants in tail water of sediment dredging.

The invention discloses a photocatalysis bio-adsorbent and a preparing method and application thereof. The photocatalysis bio-adsorbent comprises phanerochaete chrysosporium balls, graphite type C3N4 and calcium alginate, wherein graphite type C3N4 is wrapped in hyphae of the phanerochaete chrysosporium balls through calcium alginate. The preparing method comprises the steps of adding graphite type C3N4 to sodium alga acid solution for ultrasonic dispersion, then adding phanerochaete chrysosporium spore suspension, conducting uniform mixing, adding obtained mixed solution to calcium chloride solution dropwise for hardening, and conducting shake cultivation on embedding spheres obtained after hardening so as to obtain the photocatalysis bio-adsorbent finally. The photocatalysis bio-adsorbent has the advantages of being high in photocatalysis capacity, high in adsorption efficiency, low in cost, convenient to separate and recover and the like. The photocatalysis bio-adsorbent can be used for treating composite waste water containing cadmium and 2,4,6-trichlorophenol, the heavy metal cadmium and the organic pollutant 2,4,6-trichlorophenol in waste water can be removed at the same time, and adsorption degradation efficiency is high.

The invention discloses a high-efficiency aerobic composting bacterial agent for livestock and poultry manure and a method for aerobic composting thereof. The bacterial agent is composed of various strains in the following volume ratios: Bacillus licheniformis: Phanerochaete chrysosporium: Aspergillus niger Mold = 1:4~5:5~6. The aerobic composting steps are as follows: S1. Add agricultural and forestry wastes to the livestock and poultry manure, adjust the C / N of the livestock and poultry manure to 28-30, and the moisture content to 55%-60%; S2. Add the adjusted poultry waste to S1 Add sugar source to livestock manure, then add the above-mentioned bacterial agent, mix well and perform aerobic composting. After aerobic composting, the antibiotics in livestock and poultry manure degrade significantly, and at the same time greatly reduce the content of available heavy metals and resistance genes in livestock and poultry manure, and effectively control the loss of nitrogen, which has a great application prospect.

The invention relates to a phanerochaete chrysosporium mycelium growthform controlling method and belongs to the field of bioengineering. The phanerochaete chrysosporium mycelium growthform controlling method comprises the following steps: adding inorganic nanoparticles to a fluid nutrient medium of the phanerochaete chrysosporium to carry out ferment cultivation, and obtaining a mycelium group of the phanerochaete chrysosporium due to the facts that the inorganic nanoparticles react with spores and mycelia, and the inorganic nanoparticles enter into mycelial beads. The mycelium group has a good application effect in dye decolorization.

The invention discloses a method for removing a heavy metal pollutant from a water body by using phanerochete chrysosporium. The method comprises the following steps of: preparing spore suspension by using phanerochete chrysosporium spore powder; inoculating into a liquid culture medium for shake culture; filtering to obtain phanerochete chrysosporium pellets; adding the pellets into wastewater, adjusting the pH value of the wastewater, performing adsorption reaction under a constant temperature condition, filtering the wastewater after the reaction, and recovering the pellets to finish a removal process one time; cleaning the recovered pellets by using deionized water and then adding the cleaned pellets into culture solution for continuous culture so as to obtain phanerochete chrysosporium regenerative pellets; and adding the phanerochete chrysosporium regenerative pellets into heavy metal wastewater to be treated for cycling. By the method, hyphae are not required to be modified andpretreated and can be repeatedly used, the reaction condition is simple, and the water body of a complex composition can be treated.

The invention relates to engineered escherichia coli, and also provides a preparation method of engineered escherichia coli. The preparation method comprises the following steps: cloning cDNA (complementary Deoxyribose Nucleic Acid) of a gene of phanerochaete chrysosporium lignin peroxidase; performing double digestion on the cDNA; connecting the digestion fragments with a pCold-TF vector; authenticating whether the obtained plasmid pCold-TF-LiP is subjected to positive expression; and introducing the positive plasmid pCold-TF-LiP into escherichia coli BL21 competent cells to obtain the engineered escherichia coli for expressing lignin peroxidase. The invention also provides an application of the engineered escherichia coli, namely, inoculating the engineered escherichia coli into a liquid culture medium, adding ampicillin to the liquid culture medium, culturing until OD600 (Optical Density) reaches 0.58 to 0.62, and adding a protein inductive agent IPTG (Isopropyl Thiogalactoside) to induce the expression of recombinant protein. The engineered escherichia coli is high in expression quantity and extremely high in activity, and the enzyme activity reaches 3,528U / L.

The invention provides a method for preparing a biological fertilizer by brown coal humic acid. With phanerochaete chrysosporium CICC40719 as a starting strain; 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 5% of pulverized brown coal and 94.54% of deionized water is inoculated with the phanerochaete chrysosporium, which is cultured by a shaking table atthe temperature of 28 DEG C for 96 hours to prepare a bacterial culture suspension I; another 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 10% of pulverized brown coal and 89.54% of deionized water is inoculated with 5% of the bacterial culture suspensionI to prepare a bacterial culture suspension II; another 100-300ml solution containing 0.3% of KH2PO4, 0.15%g of MgSO4 7H2O, 0.01% of vitamin B1, 15% of pulverized brown coal and 84.54% of deionized water is inoculated with 5% of the bacterial culture suspension II which is cultured by the shaking table to prepare a bacterial culture suspension III which is separated and purified to obtain a phanerochaete chrysosporium acclimatized strain. A culture medium is inoculated with the culture medium and cultured for 24 hours, and fermentation broth is prepared. The brown coal is pulverized by a pulverizer and sieved by a 30-mesh sieve to obtain pulverized coal, which is added with water for regulating the moisture content of the pulverized coal to 35-40%, 12-15% of ammonia water (wet basis), and 2% of rice hull (wet basis), the wet pulverized coal is stirred evenly, spread out indoors to a thickness of 8cm, and thermostatically cultured at the temperature of 28 DEG C for 30 hours, then sulfuric acid solution with 8% percentage concentration is used for neutralizing the degraded pulverized brown coal, the pH value of which is adjusted between 6.5 and 7.5, after being dried with hot air at the temperature of 55 DEG C and pulverized, the biological fertilizer is prepared.

The invention provides a biological preparation for treating crop straw. The biological preparation is prepared from complex microbial agents and auxiliaries according to a proportion of 1-2:3-5. Fermentation cytophaga, alcaligenes faecalis, paracoccus denitrificans, bacillus pumilus, phanerochaete chrysosporium and actinomycetes are mixed with one another according to a volume ratio of 5:4:2:6:1:3 to obtain the complex microbial agents. The biological preparation has the advantages that the problems of tight crop rotation between two seasons of crops due to the fact that all straw returns to fields locally, difficulty in carrying out cuttage and seed sowing on next-stubble crops, yellowing and seedling weakness due to nitrogen fighting between seedlings and straw decomposition and the like can be effectively solved by the aid of the biological preparation; the straw can return to fields locally, and the biological preparation can be widely applied to returning the crop straw of rice, wheat, corn and the like to the fields.