High-conversion-rate production method of fermented citric acid
A technology for fermenting citric acid and high conversion rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of wheat flour fermentation effect affecting fermentation conversion rate, production and application value, unsatisfactory technical effect, etc. , to achieve the effect of improving conversion efficiency, small equipment investment and improving utilization rate
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Embodiment 1
[0023] A high conversion rate production method of fermented citric acid, comprising the steps of:
[0024] S1. Mix 100 parts of wheat flour, 15 parts of wheat straw, and 6 parts of wheat bran, crush them through an 80-mesh sieve, add 200 parts of water, and ultrasonically soak at 50°C for 5 hours. The ultrasonic power is 400W, and the concentration is 0.2mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.8, and α-amylase with a specific activity of 15 U / g and cellulase with a specific activity of 6 U / g were added for enzymolysis for 10 hours to obtain pretreated wheat flour;
[0025] S2. Filter 150 parts of pretreated wheat flour through plate and frame filtration to remove filter residue, obtain filtrate after decolorization, wherein the filtrate turbidity≤30NTU, 420nm light transmittance≥6.2%, adjust the total sugar concentration in the filtrate to 12%, then add 2 parts of sulfuric acid Ammonium, 0.5 part of potassium dihydrogen phosphate, 0.1 part of vi...
Embodiment 2
[0030] A high conversion rate production method of fermented citric acid, comprising the steps of:
[0031] S1. Mix 100 parts of wheat flour, 25 parts of wheat straw, and 2 parts of wheat bran, crush them through an 80-mesh sieve, add 400 parts of water, and ultrasonically soak at 40°C for 12 hours. The ultrasonic power is 300W, and the concentration is 0.6mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.6, and α-amylase with a specific activity of 20 U / g and cellulase with a specific activity of 2 U / g were added for enzymolysis for 20 hours to obtain pretreated wheat flour;
[0032] S2. Filter 120 parts of pretreated wheat flour through plate and frame filtration to remove the filter residue, obtain the filtrate after decolorization, wherein the filtrate turbidity≤30NTU, 420nm light transmittance≥6.2%, adjust the total sugar concentration in the filtrate to 16%, and then add 1 part of sulfuric acid Ammonium, 1 part of potassium dihydrogen phosphate, 0.01...
Embodiment 3
[0037] A high conversion rate production method of fermented citric acid, comprising the steps of:
[0038] S1. Mix 100 parts of wheat flour, 18 parts of wheat straw, and 5 parts of wheat bran, crush them through an 80-mesh sieve, add 260 parts of water, and ultrasonically soak at 47°C for 6 hours. The ultrasonic power is 370W, and the concentration is 0.3mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.7, and α-amylase with a specific activity of 18 U / g and cellulase with a specific activity of 3 U / g were added for enzymolysis for 18 hours to obtain pretreated wheat flour;
[0039] S2, filter 130 parts of pretreated wheat flour through plate and frame to remove filter residue, obtain filtrate after decolorization, wherein filtrate turbidity ≤ 25NTU, 420nm light transmittance ≥ 6.5%, adjust the total sugar concentration in the filtrate to 15%, then add 1.3 parts of sulfuric acid Ammonium, 0.8 parts of potassium dihydrogen phosphate, 0.02 parts of vitamin ...
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