High-conversion-rate production method of fermented citric acid

A technology for fermenting citric acid and high conversion rate, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of wheat flour fermentation effect affecting fermentation conversion rate, production and application value, unsatisfactory technical effect, etc. , to achieve the effect of improving conversion efficiency, small equipment investment and improving utilization rate

Inactive Publication Date: 2020-12-08
TTCA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, wheat flour is gradually being used in citric acid fermentation, and the added value of the isolated gluten flour is high, but the fermentation effect of wheat flour affects the fermentation conversion rate, and the conversion rate is lower than 93%. The technical effect achieved is not ideal, and its production Application value is severely affected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high conversion rate production method of fermented citric acid, comprising the steps of:

[0024] S1. Mix 100 parts of wheat flour, 15 parts of wheat straw, and 6 parts of wheat bran, crush them through an 80-mesh sieve, add 200 parts of water, and ultrasonically soak at 50°C for 5 hours. The ultrasonic power is 400W, and the concentration is 0.2mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.8, and α-amylase with a specific activity of 15 U / g and cellulase with a specific activity of 6 U / g were added for enzymolysis for 10 hours to obtain pretreated wheat flour;

[0025] S2. Filter 150 parts of pretreated wheat flour through plate and frame filtration to remove filter residue, obtain filtrate after decolorization, wherein the filtrate turbidity≤30NTU, 420nm light transmittance≥6.2%, adjust the total sugar concentration in the filtrate to 12%, then add 2 parts of sulfuric acid Ammonium, 0.5 part of potassium dihydrogen phosphate, 0.1 part of vi...

Embodiment 2

[0030] A high conversion rate production method of fermented citric acid, comprising the steps of:

[0031] S1. Mix 100 parts of wheat flour, 25 parts of wheat straw, and 2 parts of wheat bran, crush them through an 80-mesh sieve, add 400 parts of water, and ultrasonically soak at 40°C for 12 hours. The ultrasonic power is 300W, and the concentration is 0.6mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.6, and α-amylase with a specific activity of 20 U / g and cellulase with a specific activity of 2 U / g were added for enzymolysis for 20 hours to obtain pretreated wheat flour;

[0032] S2. Filter 120 parts of pretreated wheat flour through plate and frame filtration to remove the filter residue, obtain the filtrate after decolorization, wherein the filtrate turbidity≤30NTU, 420nm light transmittance≥6.2%, adjust the total sugar concentration in the filtrate to 16%, and then add 1 part of sulfuric acid Ammonium, 1 part of potassium dihydrogen phosphate, 0.01...

Embodiment 3

[0037] A high conversion rate production method of fermented citric acid, comprising the steps of:

[0038] S1. Mix 100 parts of wheat flour, 18 parts of wheat straw, and 5 parts of wheat bran, crush them through an 80-mesh sieve, add 260 parts of water, and ultrasonically soak at 47°C for 6 hours. The ultrasonic power is 370W, and the concentration is 0.3mol / L sodium hydroxide. The pH value of the solution was adjusted to 6.7, and α-amylase with a specific activity of 18 U / g and cellulase with a specific activity of 3 U / g were added for enzymolysis for 18 hours to obtain pretreated wheat flour;

[0039] S2, filter 130 parts of pretreated wheat flour through plate and frame to remove filter residue, obtain filtrate after decolorization, wherein filtrate turbidity ≤ 25NTU, 420nm light transmittance ≥ 6.5%, adjust the total sugar concentration in the filtrate to 15%, then add 1.3 parts of sulfuric acid Ammonium, 0.8 parts of potassium dihydrogen phosphate, 0.02 parts of vitamin ...

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Abstract

The invention discloses a high-conversion-rate production method of fermented citric acid. The production method comprises the following steps of mixing wheat flour, wheat straw and wheat bran, performing pulverizing, screening with a 80-mesh sieve, adding water, carrying out ultrasonic soaking at 40-50 DEG C for 5-12 hours at the ultrasonic power of 300-400W, regulating the pH value of the systemto 6.6-6.8, and adding alpha-amylase and cellulase to carry out enzymolysis for 10-20 hours to obtain pretreated wheat flour; filtering and decoloring the pretreated wheat flour to obtain a filtrate,adjusting the total sugar concentration in the filtrate to 12-16%, then adding ammonium sulfate, monopotassium phosphate, vitamin B1 and compound trace elements, and uniformly mixing to obtain a fermentation culture solution; inoculating phanerochaete chrysosporium, trichoderma reesia and aspergillus niger into the fermentation culture solution, introducing air for fermentation in a stirring state with the air introducing amount of 0.4-0.5VVM, performing fermenting at the temperature of 30-40 DEG C until the concentration of residual reducing sugar is less than or equal to 0.2 percent, carrying out filtering, and performing chromatographic separation and extraction.

Description

technical field [0001] The invention relates to the technical field of citric acid fermentation production, in particular to a high-conversion production method of fermented citric acid. Background technique [0002] Citric acid, scientific name 2-hydroxypropane-1,2,3-tricarboxylic acid, molecular formula C6H8O7, is an organic acid widely used in food, medicine and chemical industry. Citric acid is the largest acid among organic acids. At present, the annual production capacity of citric acid in my country has reached more than 1 million tons, accounting for about 70% of the world's production capacity; the annual output of citric acid is more than 700,000 tons, accounting for about 65% of the world's total; The annual export volume is 500,000 tons, more than half of the world's total trade volume. The world's two largest citric acid importers are Germany and the United States, with an annual import volume of about 100,000 tons and 80,000 tons. my country is the world's lar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12P39/00C12R1/885C12R1/685C12R1/645
CPCC12P7/48C12P39/00C12P2201/00
Inventor 刘海清于清吴凤宾苏晓宾
Owner TTCA
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