58results about How to "Effective hydrolysis" patented technology

The invention discloses a sludge curing agent and application thereof. The sludge curing agent is suitable for curing treatment on high-water-content sludge in town sewage treatment plants, rivers andlakes. The sludge curing agent comprises the following raw materials in percentage by weight: 26 to 44 percent of silicon aluminum iron calcium oxide, 19 to 37 percent of calcium oxide, 13 to 30 percent of special cement, 5 to 25 percent of polyacrylamide and 0.2 to 3 percent of chitosan. The sludge curing agent realizes sludge curing and stabilization through the chemical property of the industrial waste and the mutual activity excitation; the water content of the sludge is effectively reduced, the early-stage compressive strength and the flexural strength of the sludge are improved, and mechanical operation during backfilling is facilitated; heavy metal in the sludge is adsorbed by the chitosan, organic matters are degraded, the pathogene is deactivated, pollution is reduced and stink can be eliminated; and the sludge curing agent is low in price and simple in curing process, and the cured sludge does not cause secondary pollution.

The invention provides a compound enzyme preparation and a beverage prepared by using the compound enzyme preparation, and relates to the technical field of foods and beverages. The compound enzyme preparation is prepared from amylase, cellulase, papain and glutamine transaminase. The compound enzyme preparation can effectively hydrolyze starch, fiber and protein, improve the content of soluble components of the starch, fiber and protein, and catalyze intramolecular and intermolecular covalent crosslinking of oat protein or polypeptide, so that the structure of the oat protein can be changed in the enzymolysis process, bitter peptides can be hidden, umami peptides can be exposed, and the enzymolysis efficiency can be improved. The problem that an existing oat plant protein beverage can generate bitter taste is effectively relieved; meanwhile, the compound enzyme preparation also has the effect of improving the emulsifying and stabilizing performance of the oat protein beverage, and the step of adding an emulsifying agent and a stabilizing agent for blending in the later period of the existing oat protein beverage is avoided.

The invention relates to a soup clay pot and a control method thereof. The soup clay pot comprises a pot body, an inner liner which is positioned inside the pot body and is used for containing food, a heating device used for heating the food, a temperature measuring device used for measuring the temperature of the food, a sealing device used for closing an inner pot, and a control device used for controlling the soup clay pot to work; wherein the control device is internally preset with high temperature T1, maximum high temperature T1max, minimum high temperature T1min, low temperature T2, maximum low temperature T2max and minimum high temperature T2min as well as high temperature heat preservation time t1 and low temperature heat preservation time t2; temperature signals output by the temperature measuring device are compared with the preset temperature parameters by the control device, time signals output by the temperature measuring device are compared with the preset time parameters by the control device, and the action of the heating device (4) and the sealing device (6) can be controlled by the control device according to the comparison results. The soup clay pot has simple structure and convenient control, and the control method of the soup clay pot can effectively promote protein in the food to be hydrolyzed, thus being beneficial to digestive absorption of nutrition.

The invention relates to a lignocellulose anaerobic methane-producing pretreatment and fermentation technology. Pretreatment is conducted on lignocellulose through cellulase, and hydrolysis of the lignocellulose in the anaerobic fermentation process is facilitated. The technology comprises the steps that the moisture content of the raw materials is adjusted to below 10%; physical crushing is conducted on the lignocellulose through a crushing machine; the cellulase is added into the crushed lignocellulose; a buffer solution is added into lignocellulose and cellulase mixed liquor, and the solid content is controlled to blow 10%; a reaction is conducted for 72 h at 50 DEG C, and full stirring is conducted; an anaerobic fermentation inoculum is directly added into the lignocellulose and cellulase mixed liquor to be fermented, and the additive amount of dry matter of the inoculum is 50%-300% of that of the dry matter of the lignocellulose. According to the lignocellulose anaerobic methane-producing pretreatment and fermentation technology, the content of the cellulase inside a reactor can be increased, the content of the cellulase in the anaerobic fermentation process is increased, the efficiency of anaerobic fermentation is promoted, and the daily gas yield, the accumulated gas yield and the unit raw material gas yield are increased.

The invention provides a method for increasing compost humic acid content through stage treatment, belongs to the technical field of solid organic waste treatment and resource utilization, and aims to pre-hydrolyze organic solid waste to be treated and destroy a lignocellulose crystal connection structure in the organic solid waste to be treated. According to the method, the lignocellulose efficient hydrolysis microbial inoculum is added before composting to promote hydrolysis of organic components, ventilation is stopped while a functional material is added in the high-temperature stage of composting, rapid condensation of a humic acid precursor compound is promoted to form humic acid, and a substrate is provided for humic acid formation; after ventilation is recovered, the functional material stimulates enzyme activity, and oxidative polymerization of the humic acid precursor is further accelerated. In the compost cooling period, mineral substances and metal ion oxides are added into the compost body, and formed humic acid molecules are protected against further hydrolysis by microorganisms.

The invention provides a sewage treatment system and a sewage treatment method. The sewage treatment system comprises a membrane biological reaction tank. A biological rotary cage is arranged inside the membrane biological reaction tank, wherein multi-hole hollow biological balls are filled inside the biological rotary cage. Biological cotton balls are arranged inside the multi-hole hollow biological balls. The surfaces of the biological cotton balls are chelated with iron ions and are covered with biological membranes. The sewage treatment method comprises the steps that after primary treatment is carried out on sewage in regulating tanks, the sewage is transported to the membrane biological reaction tank, the biological rotary cage is activated, and the multi-hole hollow biological balls can rotate in the sewage; separation is carried out on activated sludge and macromolecule organic matters in the sewage by the biological membranes; aerobes, facultative biological floras and obligated anaerobic bacteria are added to carry out oxygenolysis on a midbody of a molecular chain; an organic compound broken chain of a long chain is converted to the midbody of the molecular chain by the iron ions; viruses and bacteria are sterilized by a high-energy physical oxidation reactor, after detecting, the standard is reached, and discharging is carried out. According to the sewage treatment system and the sewage treatment method, sewage treatment which is rapid, efficient and low in cost can be achieved.

The invention discloses a compound ethanol enzyme with acid proteases and a method for preparing the compound ethanol enzyme. The compound ethanol enzyme and the method have the advantages that the high-activity acid proteases and plant extract with abundant plant-based enzymes and nutrient substances are particularly compounded on the basis of scientific compound food-grade enzyme preparations, so that the compound ethanol enzyme is provided with complete enzyme systems; activators, protective agents and antioxidants are further added into the high-activity acid proteases and the plant extract, the enzyme activity can be completely released by the aid of the activators and can be stabilized by the aid of the protective agents and the antioxidants, and hops extract and novasil which are used for inhibiting bacteria contamination and promoting saccharification of leaven and quick proliferation and fermentation of saccharomyces cerevisiae are further scientifically compounded; starch, proteins, fat, cellulose, hemi-cellulose, pectin and the like in unprocessed grain can be sufficiently and effectively hydrolyzed, accordingly, the raw material utilization rate and the liquor yields can be effectively increased, and the alcohol contents can be increased by 13.63%, 36.25% and 17.44% respectively as compared with commercially available special compound ethanol enzymes; the liquor yields of raw materials can be respectively increased by 17.41%, 38.81% and 20.04%, and the ethanol production cost can be obviously reduced.

The invention discloses a method for quickly separating and culturing fibroblast from human skin tissues. The method comprises the following steps of cleaning and shearing fresh foreskin tissues afteroperation of adults or children, and sequentially adopting solutions of dispase II and dispase I to digest under the condition of oscillation in a water bath at the temperature of 37 DEG C; and collecting skin tissue blocks through centrifuging; enabling a culture medium to resuspend, and culturing in a culture box with CO2 (carbon dioxide) volume concentration of 5% at the temperature of 37 DEGC. The method has the advantages that the temperature of the digestion function of the dispase I and the dispase II is increased from 4 DEG C to 37 DEG C, the activities of the dispase I and the dispase II are respectively improved, the contact areas between the dispase I as well as the dispase II and a corresponding primer are increased by the oscillation in the water bath, the enzymatic reaction is favorably performed, the digestion time of the dispase I as well as the dispase II is greatly shortened, and the separating and culture speed of the fibroblast is improved; the damage of enzyme to tissue cells is decreased, the wall attaching rate of the cells is improved, and the primary fibroblast can complete passage after culturing for 6 to 8 days.

The invention discloses a production method and applications of a novel rhizomucor miehei aspartic protease, and particularly relates to a recombinant aspartic protease, a coding gene, and a production method and applications of the recombinant aspartic protease. The recombinant aspartic protease is protein having an amino acid sequence shown as SEQ ID NO:1. The coding gene of the recombinant aspartic protease is a DNA molecule shown as SEQ ID NO:2. An aspartic protease gene that is RmproA of rhizomucor miehei is connected to a pichia pastoris GS115 expression vector pPIC9K, and is converted into pichia pastoris and induced expression is performed to obtain the recombinant aspartic protease. Through high-density fermentation in a fermentation tank having a volume of 5 L, the maximum protease production activity at 156 h of a recombinant strain is 3400 U/mL, and the protein content is 6.42 mg/mL. The recombinant aspartic protease is capable of effectively tendering pork, reducing shearing force, making meat palatable, and effectively hydrolyzing animal and plant protein to prepare low-molecular-weight polypeptides.

The invention discloses a Baijiu-brewing compound enzyme containing acid protease and a preparation method of the Baijiu-brewing compound enzyme containing the acid protease and belongs to the technical field of compound enzyme preparation. The Baijiu-brewing compound enzyme containing the acid protease and the preparation method have the advantages that on the basis of scientific compounding of food-grade enzyme preparations, high-activity acid protease and plant extracts containing plant enzymes and nutrient substances are compounded specially to enable an enzyme system of the compound enzyme to be more complete; activating agents enabling enzyme activity to be completely released and protective agents and antioxidants enabling enzyme activity to be more stable are added, and esterified red yeast and aroma-producing active dry yeast which make Baijiu more sufficient in aroma are compounded scientifically, so that starch, protein, fat, cellulose, hemicellulose, pectin and the like in grain can be hydrolyzed sufficiently and effectively, Baijiu yield can be increased by 24% to 29% remarkably, total ester content can be increased from 16.59% to 57.85%, and ethyl acetate content can be increased from 17.9% to 109%; taste of various types of Baijiu can be improved remarkably, and the compound enzyme can be suitable for brewing various types of Baijiu and addition of different Baijiu brewing methods.

The invention relates to the technical field of bactericide compounding, and discloses a bactericide for afforestation tree disease damage prevention and control and a preparation method thereof. Effective ingredients of the bactericide are mainly prepared from Chinese pulsatilla roots, black nightshade herb, artemisia annua, herba violae, radix stemonae, garlic, radix clematidis, fructus chebulae, cortex phellodendri, an adhesive, a thickening agent, a lubricating agent and water. The preparation method of the bactericide mainly comprises the steps of washing, filtering, preparing and the like. The bactericide has a good disease damage prevention and control effect on common urban afforestation disease damages such as anthracnose, powdery mildew and a rust disease; in addition, all the adopted main ingredients are Chinese herb plants beneficial for a human body and the environment and are good in proportioning effect, friendly to urban ecology, free of toxic and side effects on people and livestock and high in using safety; the preparation method of the bactericide is easy and convenient to operate and can be circularly put into use, and therefore the method is suitable for being applied and popularized in urban afforestation projects.

The invention relates to a preparation method of a sausage, concretely relates to a duck blood polypeptide nutrient sausage and its preparation method, and belongs to the technical field of food processing. The method comprises the following steps: carrying out fermenting extraction of fresh duck blood to obtain a duck blood polypeptide nutrient solution; adding small amounts of starch, a vegetable gelatin, and assistants comprising salt, sugar, pepper and Chinese prickly ash; and filling to form a sausage, boiling, and cooling to prepare the duck blood polypeptide nutrient sausage, wherein the raw material duck blood is blood from domestic ducks, has a salty taste and a cold property, and can enrich the blood and detoxify. The fresh duck blood contains 16.26% of proteins, 226mg/kg of Fe, 5.77mg/kg of Zn, and 12.82% of amino acids, so the duck blood is a biological resource having very high nutritive values, and the finished products of the duck blood are especially suitable for special consumption populations comprising humans suffering from the alimentary canal disease, hypoallergenic patients, the elderly, athletes and the like.

The invention relates to the field of genetic engineering, in particular to low-temperature alpha-galactosidase GalA17, a gene thereof and application thereof, and provides an alpha-galactosidase GalA17 derived from flavobacterium sp. TN17 in gastrointestinal tracts of long-horned beetles, and the amino acid sequence of the alpha-galactosidase GalA17 is shown as SEQ ID No.1. The invention provides an encoding gene galA17 for encoding the alpha-galactosidase. The alpha-galactosidase has the following properties that: the optimum pH is 5.5, the optimum temperature is at 45 DEG C, and enzyme activity is over 55 percent at the temperature of between 30 and 50 DEG C; the pH stability is excellent; the protease resistibility and capability of hydrolyzing various substrates are excellent; and the alpha-galactosidase can be used as feed or food additives applied in feed and food industry.

The invention discloses a composite enzyme used for feeding piglets. The composite enzyme comprises 100000 to 200000 U/g of xylanase, 8000 to 12000 U/g of dextranase, and 8000 to 12000 U/g of mannase. The invention also discloses a piglet corn-soybean meal ration containing the provided composite enzyme used for feeding piglets. With the composite enzyme provided by the invention, non-starch polysaccharides in rations can be effectively hydrolyzed; nutrients such as proteins and starches in the rations can be effectively digested and absorbed by animals; piglet growth can be promoted; cultivation cost can be reduced; cultivation environment can be reduced; and substantial economic and ecological benefits can be brought in.

The invention relates to a resveratrol zymophyte and application thereof. Classification of a bacterial strain is named as Bacillus-safensis, a storage unit is a general microorganism center of China Committee for Culture Collection of Microorganisms, preservation number is CGMCC No.13129, preservation address is No.3, No.1 Compound, Beichen West Road, Chaoyang District, Beijing, preservation date is October 21, 2016, and the application of the resveratrol zymophyte in fermented resveratrol is provided. A microbial transformation method is used for directly converting polydatin in polygonum cuspidatum crude medicinal materials to resveratrol. The method is simple, transformation process is easy to control, specificity of transformation reaction is stronger, separation and purification of products are convenient, yield and quality of resveratrol product are effectively improved, and added value of products is improved.

The invention provides a flavored function konjac sauce rich in decanoyl acetaldehyde and a preparation method of the flavored function konjac sauce. The preparation method comprises the following steps of (1) preparing root and leaf juice from fresh heartleaf houttuynia roots and heartleaf houttuynia leaves, adding deacetylate konjac glucomannan to obtain heartleaf houttuynia herb konjac gum liquid, and performing refrigeration for standby application; (2) selecting entire soybeans, performing hulling, performing washing, performing soaking, performing draining, adding the heartleaf houttuynia herb konjac gum liquid in a certain weight ratio, and performing refining to obtain viscous heartleaf houttuynia herb konjac soybean milk; (3) making the viscous heartleaf houttuynia herb konjac soybean milk into heartleaf houttuynia herb konjac bean curd blanks, performing dicing, performing vacuum dehydrating, and controlling the water content; and (4) performing inoculating with aspergillus oryzae for culturing, performing aerobic fermentation to obtain konjac bean curd yeast blanks, putting the konjac bean curd yeast blanks in a preserving machine to making preserved blanks, performing inoculating with lactic acid bacteria and microzymes, and performing anaerobic fermentation, so as to obtain the konjac sauce. The flavored function konjac sauce has unique fishy fragrance and rich flavor, can diminish inflammation, resist bacteria, promote urination and reinforce immunity of human bodies. The content of the decanoyl acetaldehyde in the konjac sauce is greater than 0.1g/Kg, the content of methyl-nonyl-ketone is greater than 0.21g/Kg, and the content of total flavonoids is greater than 6.0g/Kg.

The present invention relates to novel xylanase having alkali resistance, which corresponds to glycoside hydrolase family 10 (GH10) isolated from Micobacterium sp. HY-17 strain. More specifically, the xylanase is a novel enzyme which is stable in an alkali environment, effectively hydrolyzes various xylans, hydrolyzes beta-glucan, and degrades cellobiose. Thus, the zylanase of the present invention can be used as an enzyme in the food industry, an enzyme for feed, a biomass pretreatment enzyme, and a useful enzyme for various industry groups including a pulp process.

The invention discloses a method for removing sticky substances in waste paper pulp by utilizing high-temperature esterase. The esterase is esterase EstE1, PDB ID: 2C7B from a metagenome library. The method specifically comprises the following steps: adding 0.1U/g to 1.0U/g (relative to absolute dry pulp mass) of the esterase into the waste paper pulp, wherein the treatment time is 45min to 120min, the temperature is 40 DEG C to 80 DEG C, the pH (Potential of Hydrogen) is 5.0 to 9.0, and the stirring speed is 100rpm to 150rpm; finally, detecting the content of the sticky substances in the waste paper pulp according to a TAPPIT-277 standard method. A result proves that the removal rate of the sticky substances reaches 54.1 percent to 66.5 percent; the removing effect in a high-temperature environment is still relatively good. The sticky substances in the waste paper pulp are effectively degraded through utilizing the high-temperature esterase EST1; an operation process is simple and convenient, and extra mechanical equipment is not needed; the removing effect on the sticky substances is obvious, the production cost is low, and no pollution is caused.

The invention discloses a high-conversion-rate production method of fermented citric acid. The production method comprises the following steps of mixing wheat flour, wheat straw and wheat bran, performing pulverizing, screening with a 80-mesh sieve, adding water, carrying out ultrasonic soaking at 40-50 DEG C for 5-12 hours at the ultrasonic power of 300-400W, regulating the pH value of the systemto 6.6-6.8, and adding alpha-amylase and cellulase to carry out enzymolysis for 10-20 hours to obtain pretreated wheat flour; filtering and decoloring the pretreated wheat flour to obtain a filtrate,adjusting the total sugar concentration in the filtrate to 12-16%, then adding ammonium sulfate, monopotassium phosphate, vitamin B1 and compound trace elements, and uniformly mixing to obtain a fermentation culture solution; inoculating phanerochaete chrysosporium, trichoderma reesia and aspergillus niger into the fermentation culture solution, introducing air for fermentation in a stirring state with the air introducing amount of 0.4-0.5VVM, performing fermenting at the temperature of 30-40 DEG C until the concentration of residual reducing sugar is less than or equal to 0.2 percent, carrying out filtering, and performing chromatographic separation and extraction.

The invention discloses a method for preparing degummed mulberry silk products based on a biological enzyme method, which belongs to the technical field of dyeing and finishing. In the present invention, protease and cutinase are used to treat mulberry silk products to remove sericin and hydrophobic lipids on the surface of mulberry silk; then, lipase, glycerol triacetate and hydrogen peroxide are added to the system to remove silk The glyceride remaining on the surface of the plain, catalyzing the hydrolysis of glycerol triacetate to produce acetic acid at the same time, the acetic acid and hydrogen peroxide react to produce peracetic acid, and the low-temperature bleaching of mulberry silk is carried out to realize the method of degumming processing of mulberry silk products by enzymatic method. Compared with the traditional rapid scouring method of alkali method and only protease degumming method, the method of the present invention has low production energy consumption, good degumming effect and reduced environmental pollution. The degummed silk product prepared by the method of the invention has a degumming rate of more than 23.5%, a whiteness of more than 82.5%, a wetting time of less than 1s, a strength of more than 562N and a COD of less than 1751mg/L.

The preparation method comprises the following steps: (1) dissolving a soluble nickel salt, a soluble cobalt salt, urea and ammonium fluoride in deionized water to form a uniform solution, transferring the uniform solution into a reaction kettle containing carbon cloth, carrying out a hydrothermal reaction, washing the reaction kettle with water, and drying the reaction kettle to obtain a nickel-cobalt nitride nanosheet array; a nickel-cobalt bimetallic precursor loaded on the carbon cloth is obtained; and (2) placing the nickel-cobalt bimetallic precursor loaded on the carbon cloth in an ammonia atmosphere for high-temperature nitridation to obtain a target product, namely the difunctional electrocatalyst. Based on an in-situ growth strategy and an array structure of the heterojunction nanosheets, rapid electron transfer is facilitated, more active sites are exposed, electron interaction of a heterojunction interface and a synergistic effect of different components are facilitated, and therefore the catalytic activity of the material is facilitated.

The invention relates to a technical triazophos floating stabilizing bag taking silicon gel as an adsorbent. The technical triazophos floating stabilizing bag comprises a stabilizing bag made of nonwoven cloth; activated silicon gel is wrapped in the stabilizing bag; the periphery of the stabilizing bag is connected with a buoy; the stabilizing bag is kept on the upper layer of technical triazophos by virtue of the buoyancy of the buoy. The technical triazophos floating stabilizing bag provided by the invention has the beneficial effects that the stabilizing bag with an adsorption effect floats on the top of the technical triazophos by virtue of the buoyancy of the buoy, and does not sink to the bottom, thus furthest and fast adsorbing various impurities such as water and ethyl alcohol, and effectively improving the stabilizing time of the technical triazophos.