The invention discloses a production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through a complex enzyme method.The production technology comprises the following steps that1, the medicinal material cortex phellodendri or coptidis rhizoma is smashed, and water is added to the powder for soaking; 2, beta-dextranase, xylanase, phytase and cutinase are added into the mixture, the pH is adjusted with diluted hydrochloric acid, enzymolysis and boiling are conducted, enzymes are subjected to inactivation, and enzymatic hydrolysate is obtained; 3, water is added, the pH is adjusted with diluted hydrochloric acid, extraction is conducted, hot filtration is conducted, and filtrate is collected; 4, ethyl alcohol is added into filter residues, and digestion is conducted; 5, filtrates are combined, concentration is conducted, the pH is adjusted with diluted hydrochloric acid, cooling is conducted, a saturated NaCl solution is added, overnight preservation is conducted at 4 DEG C, suction filtration is conducted, drying is conducted, and berberine is obtained.The production technology for extracting berberine from cortex phellodendri and coptidis rhizoma through the complex enzyme method is simple in method, high in product yield and purity, low in cost and free of environmental pollution.