5 results about "Cutinase" patented technology

The present invention relates to a method for treating a textile comprising a polyester with low crystallinity by contacting the textile with a cutinase, as well as a textile produced by such method.

This invention discloses a BhrPETase keratinase mutant and its application in the degradation of polyethylene terephthalate (PET), belonging to the fields of environmental science and enzyme engineering. The site numbers are based on the amino acid sequence shown in SEQ ID NO:1. The mutant contains substitutions of S14G, H78Y, and S184G relative to SEQ ID NO:1, with the preferred amino acid sequence shown in SEQ ID NO:9. Under conditions of 1 mg enzyme protein / g substrate, 65°C, and 96 h, the preferred mutant exhibits a degradation rate of 90.4% for PET membranes and a half-life of 150 h at 60°C, making it suitable for enzymatic degradation and depolymerization of PET.

The application discloses a wool tricarboxylic acid blended fabric and a preparation method thereof, and belongs to the technical field of textile printing and dyeing. The method comprises the following steps: respectively performing normal-pressure low-temperature plasma pretreatment and specific enzyme synergistic modification on wool sliver and tricarboxylic acid short fiber sliver, moderately passivating wool scales by using protease, and promoting surface micro-deacetylation of tricarboxylic acid by using esterase or cutinase; mixing the slivers, spinning and weaving into a fabric; performing one-bath same-color dyeing by using disperse / acid dyes at 100 DEG C under normal pressure; and performing medium-low temperature heat setting. Through synergistic effect of plasma physical etching and biological enzyme chemical degradation, the technical prejudice that tricarboxylic acid needs high-temperature dyeing at 130 DEG C is broken. The method can obtain excellent same color under normal pressure, significantly improves wool strength retention rate, avoids wool heat damage and yellowing, the finished product has low shrinkage rate after machine washing, is green and environment-friendly, and is suitable for industrial popularization.

The application discloses a method for improving catalytic degradation of PET plastic by cutinase, and the method comprises the following steps: preparing a high-molecular condensed phase, collecting a concentrated phase of the condensed phase by centrifugation, adding LCC-ICCG-218Y enzyme solution and PET solid to the concentrated phase, and oscillating to make the concentrated phase uniformly distributed in the reaction system, starting the reaction under oil bath at 40-72 DEG C, collecting the reaction liquid, and characterizing the degradation product of the reaction liquid by ultraviolet. Compared with a phosphate buffer solution, the degradation rate of PET plastic at 40 DEG C and 50 DEG C is finally doubled. The method establishes a simple method for improving enzymatic hydrolysis of PET plastic, and only by adjusting the reaction microenvironment of the enzyme, without complex protein modification and enzyme immobilization means, the method has certain application value.