Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for treating papermaking white water by utilizing cutinase

A technology of cutinase and processing time, which is applied in the field of enzyme engineering, can solve the problems of high processing cost, downtime, paper quality, and large chemical consumption, and achieve the effects of improving production efficiency, mild reaction conditions, and simple production

Active Publication Date: 2017-03-08
JIANGNAN UNIV
View PDF10 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Resin substances in white water DCS are some hydrophobic substances soluble in neutral organic solvents. These substances will be deposited on the surface of equipment in various forms during the papermaking process, causing problems such as downtime and paper quality decline.
[0004] The common treatment method for this kind of problem is mainly to use cationic polyelectrolyte for neutralization. This process not only needs to consume a lot of chemicals, but also has high treatment cost, and will increase the difficulty of subsequent water treatment in papermaking. Therefore, it is necessary to develop a mild reaction condition, Environmentally friendly papermaking white water treatment method is very necessary

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for treating papermaking white water by utilizing cutinase
  • Method for treating papermaking white water by utilizing cutinase
  • Method for treating papermaking white water by utilizing cutinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Thermobifidafusca-derived cutinase hydrolysis of papermaking white water resinous deposits (glycerol trioleate) model

[0029] Weigh 15 mL of triolein (0.915 g / mL) and add it to 85 mL of Tris-HCl buffer solution at pH 8.0. After mixing evenly, take 10 mL and add 100 U of Thermobifidafusca-derived cutinase (Genbank accession number: AAZ54920), in a 60°C water bath After 30 minutes of accurate reaction in medium, take 5mL of reaction solution and add 5mL of 95% ethanol to terminate the reaction, and then use 0.05mol / L sodium hydroxide standard solution to titrate to pH 10.3 on the potentiometric titrator as the end point. According to the sodium hydroxide standard solution consumed by titration The volume of cutinase to judge the decomposition of triolein. Cutinase can decompose triolein to generate oleic acid. By titration, the more sodium hydroxide consumption is consumed, the more thorough the enzyme reaction is. The results are shown in the table As shown i...

Embodiment 2

[0032] Example 2: Fusarium solani-derived cutinase hydrolyzes papermaking white water resinous deposits (glycerol trioleate) model

[0033] Weigh 15 mL of triolein and add it to 85 mL of Tris-HCl buffer solution at pH 8.5. After mixing evenly, take 10 mL and add 1000 U Fusarium solani-derived cutinase (Genbank accession number: AAA33334), and react in a 60°C water bath for 60 minutes Finally, take 5 mL of sample and add 5 mL of 95% ethanol to terminate the reaction, and then use 0.05 mol / L sodium hydroxide standard solution to titrate on a potentiometric titrator to pH 10.3 as the end point. According to the volume of sodium hydroxide standard solution consumed by titration, the decomposition of triolein by cutinase is judged. The results are shown in Table 2. When the amount of cutinase added to the reaction solution was 728.6U / g substrate, the degradation rate of triolein reached 74.8% after reacting at 40°C for 60 minutes.

[0034]Table 2 Cutinase from Fusarium solani hydr...

Embodiment 3

[0036] Example 3: Humicola Insolens-derived Cutinase Hydrolysis of Papermaking White Water Resin Deposits (Glyceryl Trioleate) Model

[0037] Weigh 15 mL of triolein and add it to 85 mL of Tris-HCl buffer solution at pH 8.5. After mixing evenly, take 10 mL and add 200 U of cutinase from Humicola Insolens (RCSB Protein data bank accession number: 4OYY), in a water bath at 40 °C After accurate reaction for 60 minutes, take 5 mL of sample and add 5 mL of 95% ethanol to terminate the reaction, and then use 0.05 mol / L sodium hydroxide standard solution to titrate to pH 10.3 on the potentiometric titrator as the end point. According to the volume of sodium hydroxide standard solution consumed by titration, the decomposition of triolein by cutinase is judged. The results are shown in Table 2. When the amount of cutinase added was 145.72U / g of the substrate reaction solution, the degradation rate of triolein reached 83.5% after reacting at 60°C for 60 minutes.

[0038] Table 2 Hydrol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for treating papermaking white water by utilizing cutinase and belongs to the technical field of enzyme engineering. The method disclosed by the invention adopts efficient and stable cutinase for carrying out enzyme catalytic decomposition on lipids such as glycerol trioleate and polyvinyl acetate, high temperature heating or vigorous stirring does not need to be carried out, reaction conditions are mild, and degradation rate can reach 83.5%, thereby being environment-friendly. Production of cutinase required by reaction is simple, and production efficiency is improved. Meanwhile, compared with other physical treatment, enzymatic method treatment is simple in steps, and in the actual white water treatment process, when the dose of cutinase is 0.5-1U per g substrate, better treatment effect also can be obtained, and turbidity difference reaches more than 95%. The white water treated by cutinase can be directly reused and partially or completely replaces cleaning water, so that closed circulation of papermaking system water is realized, and zero discharge in real sense is achieved. The advantages become especially important in the modern society with environmental protection taken seriously increasingly.

Description

technical field [0001] The invention relates to a method for treating papermaking white water with cutinase, which belongs to the technical field of enzyme engineering. Background technique [0002] Worldwide, wastewater from the paper industry is a source of concern. In our country, papermaking wastewater pollution has become a key factor restricting the survival and development of the industry. Pulping and papermaking wastewater mainly includes three categories: black liquor, mid-stage water, and papermaking white water. After replacing the traditional caustic soda process with clean processes such as "alkaline hydrogen peroxide steaming and bleaching integration" in the pulping system, the discharge of pulping wastewater has been low (<60m 3 / ton of pulp), its pollution load is equivalent to about 50% of the traditional process, and the advanced pulping and papermaking system only discharges steaming and bleaching wastewater and papermaking white water. Compared wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): D21F1/66
CPCD21F1/66
Inventor 吴敬洪若宇宿玲恰陈晟
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com