4024 results about "Standard solution" patented technology

The invention discloses an analysis and detection method for impurity elements such as aluminum, calcium, ion, molybdenum, niobium, titanium, tungsten and the like in chromium carbide. The method comprises adding a chromium carbide sample into a dissolving cup, adding hydrofluoric acid, sulphuric acid and nitric acid sequentially, stirring, charging into a sealed high-pressure jar; putting the sealed high-pressure jar into a microwave extinguishing instrument for two times of microwave extinguishment; taking the high-pressure jar out of the microwave extinguishing instrument for cooling, transferring the dissolved chromium carbide liquid sample into a volumeric flask, diluting to a predetermined index, stirring; preparing a chromium substrate matched mixed standard solution series of aluminum, calcium, iron, molybdenum, niobium, titanium and tungsten; measuring element emission power of aluminum, calcium, iron, molybdenum, niobium, titanium, tungsten or the like in a blank liquid sample, a chromium carbide liquid sample and the prepared series mixed standard solution by an inductively coupled plasma atomic emission spectrometer in the same time, obtaining the analysis result by checking a standard working curve or by linear equation calculation. The invention adopts two times of microwave extinguishment using the mixed acid, solves the problem of hardness in chromium carbide decomposition, having a measurement range from 0.010% to 1.00%, which is high in accuracy, and good in precision.

The invention relates to a method for extracting suaeda salsa flavone and measuring content of flavone in suaeda salsa. The method comprises the following steps: 1), collecting the suaeda salsa, drying the suaeda salsa and crushing the suaeda salsa; with ethanol as an extractant, soaking the suadeda salsa and carrying out microwave-assisted extracting; filtering; extracting with petroleum ether; transferring the ethanol layer to a macroporous adsorption resin layer; and eluting with water and ethanol aqueous solution sequentially; collecting the eluant, and pressurizing and concentrating the eluant to obtain flavone compound extract; 2), with rutin as a contrast, preparing standard solution with different concentration gradients; measuring the chromatographic peak area at the wavelength of 360nm through a high efficiency liquid chromatography to form flavone content contrast reference; measuring the chromatographic peak area of the to-be-measured solution; obtaining the flavone content of the to-be-measured solution according to the flavone content contrast reference; and obtaining the content of the flavone in the suaeda salsa according to the dilution multiple of the to-be-measured solution. According to the method provided by the invention, soaking and microwave-assisted extracting are adopted to extract flavone, and the content of the flavone is measured by chromatography, so that the method is simple and the solvent is easily available. Besides, the obtained flavone is high in content, and has the physiological and pharmacological activities such as antiviral activity, antioxidant activity, anti-ageing activity and the like.

According to the standard solution and the determination method of the present invention, in a case where a voltage is applied by a drive voltage of a measurement apparatus to an electrode portion of a biosensor comprising an electrode portion including a counter electrode and a measuring electrode formed on an insulating substrate, and a reagent layer which reacts with a sample solution supplied to the electrode portion, and a current value which flows at the application is measured, thereby determining a substrate contained in the sample solution, a reducing substance is contained in the standard solution used for controlling a precision of measurement of the measurement apparatus. Therefore, when the standard solution is measured, a large change occurs in a current waveform between time t0 and t1 shown in FIG. 6 due to the reducing substance, thereby discriminating whether the analyte liquid being measured is the standard solution or the sample solution and easily identifying the kind of analyte liquid.

The invention relates to a judging method of the solid insulating and aging degrees of a transformer, which sequentially comprises the following steps of: 1. preparing a gradient concentration standard solution of a furfural solution, carrying out liquid chromatographic detection, and making a standard curve I; 2. taking insulating paper samples in an actual electric network transformer, carrying out the liquid chromatographic detection, and obtaining the furfural concentration in each sample according to the standard curve I; 3. respectively measuring the water content H thereof by adopting the insulating paper samples in the step 2, computing the dry paper concentration C, additionally sampling, measuring the specific viscosity thereof, and computing the polymerization degree of the insulating paper samples according to a Martin empirical formula; 4. making a standard curve II by using the polymerization degree of insulating paper as an abscissa and using the logarithm of the furfural concentration as an ordinate; and 5. measuring the furfural concentration for an unknown sample, obtaining the polymerization degree of the insulating paper according to the standard curve II in the step 4, and judging the solid insulating and aging degrees of the transformer according to an IEC60450 standard. The method only needs to extract proper oil samples, has simple operation, does not need power failure, and is a quite practical method.

The invention provides a method for detecting trace elements in human body whole blood through an inductively coupled plasma mass spectrometry method and belongs to the technical field of biotechnical detection. The method comprises the following steps: (1) pre-treating a whole blood sample; (2) detecting a mixed element standard solution and a to-be-detected sample solution by an inductively coupled plasma mass spectrometer; and (3) performing quantitative analysis on a detection result and calculating the content of to-be-detected elements in the human body whole blood sample. The method provided by the invention can be used for simultaneously detecting the content of trace elements, such as magnesium, manganese, calcium, iron, copper, zinc and lead, in the human body whole blood; the sample dosage is less; the pretreatment is simple; the cost is low; the whole detection process is short in time and high in efficiency; the detection sensitivity and precision andthe detection stability are high.

The invention relates to a method for detecting the element content in alloy or ore by utilizing an ICP emission spectrometer, comprising the following steps: placing the alloy or the ore in solvent,heating and leading the alloy or the ore to be dissolved fully in solution to obtain mixed solution; diluting the mixed solution to obtain test solution; selecting the optimal analysis line of each element according to the composition of substrate of the alloy or the ore; preparing a set of corresponding standard solutions with the concentration sequencing from low to high according to the concentration index of each element in the alloy or the ore; introducing the standard solutions to the ICP emission spectrometer to measure the emission light intensity of the element analysis line and to draw a standard curve; introducing the test solution to the ICP emission spectrometer to measure the emission light intensity corresponding to each element; and determining the content of each element according to the standard curve. The method can detect simultaneously the content of the required element and oxide only by one solution, therefore, the invention has simple operation, shortened measuring cycle, stable detection results with high accuracy degree.

The invention relates to a fast preparation method of a biological affinity copper nanometer cluster, is applied to the detection of Hg<2+> content in a water sample, and belongs to the technical field of nanometer material preparation. A copper source solution and a protein solution are uniformly mixed; the pH of the solution is regulated to the alkalinity by alkali; oxidizing agents are dropped; heating incubation is carried out for a period of time; the copper nanometer cluster is prepared; the copper nanometer cluster is used for detecting the fluorescence intensity of an Hg<2+> standard solution or an Hg<2+> sample solution; the fluorescence intensity is substituted into a linear regression equation, and the Hg<2+> content in the sample is calculated. Due to strong oxidizing property and coordinating capability of oxidizing agents, the secondary structure in protein can be changed, copper-protein-oxidant complexes can be formed, the reducing capacity of the protein is enhanced, and the formation of the copper nanometer cluster is obviously accelerated. The copper nanometer cluster is used for the Hg<2+> detection in the water sample, and the method is obviously superior to the prior art in the aspects of analysis time, sensitivity, selectivity and cost.

The invention discloses a general rapid detection method for micromolecular poisonous and harmful substances in liquid milk. The method is characterized in that the method concretely comprises the following steps: 1, carrying out simple and general extraction and purification on a liquid milk sample to obtain a loading liquid; 2, preparing a mixed standard solution and a matrix addition standard curve; 3, determining the micromolecular poisonous and harmful substances in the liquid milk through UPLC-MS/MS; and 4, carrying out concentration calculation. The method of the invention has the following advantages: the method starts from general characteristics of micromolecular compounds and fully takes the dissolvability, the thermal stability, the acidic and basic stability, the polar strength, the coexistent stability and the like of all the substances into consideration, a general rapid preprocessing technology and a UPLC-MS/MS electro-spray apparatus determination technology are developed, and the substantial efficiency improvement and the detection cost reduction effect are brought to the production process control and food detection work on condition that detection limit requirements are satisfied.

The invention discloses a high-throughput detection method for 99 residual veterinary drugs in animal-derived food. The detection method is characterized by comprising the following concrete steps: (1) carrying out extraction and purification on 99 residual veterinary drugs having substantially different physicochemical properties and belonging to eight kinds of common veterinary drugs through one-step pretreatment based on carrier-assisted liquid-liquid extraction technology; (2) preparation of a mixed standard solution and a matrix standard curve; and (3) determining the concentrations of 99 residual veterinary drugs in a to-be-detected solution by using ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry. According to the invention, the pretreatment and instrumental analysis process of the method is directed at compounds with different physicochemical properties, so the method has the advantages of good compatibility, high detection efficiency, good operability and low detection cost, and the detection limit of the method can meet requirements of all the test objects.

In a clinical laboratory of a hospital, an enormous amount of effort has been required to maintain the quality of an analyzer, standard solution and control samples. An object of the present invention is to provide a control method for controlling a clinical laboratory with reduced cost, and a control apparatus using the same.

In order to control data of an analyzer, standard solution, and a control sample, a support center is connected to each analyzer located in each hospital through a network line. Various kinds of analysis parameters and the result of measurements are exchanged so as to provide each clinical laboratory with a control situation in real time.

The invention relates to a preparation method of a lithium ion battery cathode multicomponent composite material Lia(NixCoyMnz)NbO2 / M, and a preparation method of a precursor of the multicomponent material. The lithium ion battery cathode multicomponent composite material belongs to a nickel cobalt lithium manganate series cathode material, and can be expressed by a chemical formula Lia(NixCoyMnz)NbO2 / M. The preparation method comprises the following steps: measuring to prepare a standard mixed solution; under the protection of an inert atmosphere, adding a proper amount of a complexing agent and a precipitating agent into the mixed solution, adjusting the pH value to reach the end point of the reaction, filtering, washing and drying to obtain a spherical multicomponent precursor containing doped elements; grinding a lithium source and multicomponent precursor obtained in the step (2) in a ball mill; and placing the mixture obtained in the step (3) in a high temperature kiln to obtain a first sintering, so as to obtain the multicomponent material. The invention thorough mixing of precursor and lithium salt improves reaction activity, and strictly controls the temperature in the process of sintering, so as to obtain the multicomponent cathode material with regular morphology and uniform particle size.

The invention discloses a medical type all-solid potassium ion selectivity sensor and a preparation method thereof. The medical type all-solid potassium ion selectivity sensor comprises a substrate, two potassium ion selectivity working electrodes which are completely the same in structure and arranged side by side, an insulating layer and a salt bridge. Without a constant-potential reference electrode in the conventional sense, one of the two potassium ion selectivity working electrodes is used to be matched with a potassium standard solution to realize the function of a reference electrode, the complexity in preparation of the whole sensor is greatly reduced. Each of the two same potassium ion selectivity working electrodes comprises a conductive layer, a reaction electrode, a contact electrode and a potassium ion sensitive film. According to the invention, since the constant-potential traditional reference electrode and the electrolyte layer of a common solid-state electrode are not needed, the preparation process and flow of the all-solid potassium ion selectivity sensor are greatly simplified; and moreover, the all-solid potassium ion selectivity sensor has accurate and quick response, and is small and light, convenient to carry and suitable for large-scale production.

The invention discloses an environmental aerosol direct sampling gathering sample injecting device and a quantitative analysis method. The environmental aerosol direct sampling gathering sample injecting device comprises an environmental aerosol sampling module, an aerosol atomization dissolving generation module, an aerosol condensation growing up module, an aerosol number concentration gathering module and a base body gas removing module. The environmental aerosol sampling module comprises a sampling port, a sampling pump and a connection pipeline, preferably, the sampling pump is a membrane pump; the aerosol atomization dissolving generation module comprises an atomizer, a fog chamber, a drying device and a connection pipeline, the atomizer is used for atomizing a standard solution to generate fog drops, the fog room is used for removing large fog drops and the drying device is used for dissolving the fog drops output from the fog room and removing steam contained in the fog drops. Due to the mode that the environmental aerosol direct sampling gathering sample injecting device serves as an aerosol sample injection system and is used in cooperation with an ICP-MS, quality sample injection rate of an element to be measured in environmental aerosol can be measured quantitatively, and then sample injection volume flow of the environmental aerosol is divided by the quality sample injection rate to acquire the quality volume concentration of the element to be measured.

The invention relates to a method for detecting an organophosphorus multi-pesticide residue in tea based on matrix effect compensation, which belongs to the technical field of safety detection of foods. The method comprises the following steps of: (1) mixing a pesticide standard solution; (2) preparing a sample solution; (3) detecting a sample to be detected by a gas chromatogram and mixing the standard solution. The method is rapid, simple and economic, has high sensitivity, effectively reduces or eliminates the interference of a complex matrix derived from the tea on the detection of organophosphorus pesticide residues and provides a scientific basis for further improving an evaluation system of the quality safety of the tea.

The invention relates to a quantitative measuring method of a lithium ion battery electrolyte solvent, which relates to a quantitative measuring method of a battery electrolyte solvent, and solves the problems of the existing lithium ion battery electrolyte solvent and the additive quantitative analysis method that the requirement on detection equipment is high, the price is high, the detection process is complicated, the quantitative detection accuracy is low and the detection is easily subjected to the interference of impurities. The measuring method comprises the following steps: I, preparing an inner standard solution identical in internal standard substance concentration; II, drawing an internal standard working curve; III, preparing a detection solution; and IV, calculating the concentration of a component in the detection solution according to a specific value Y of the chromatographic peak area to the internal standard substance chromatographic peak area. The method is suitable for detecting a great number of analysis samples and has advantages of high detection speed, little time, high efficiency and the like.

The invention discloses a method that can simultaneously analyze various elements, aims at solving the defects that currently the analysis of various elements cannot be finished at one time, and consequently the analysis time is long and the reagent usage is large, and discloses a method that can simultaneously measure the elements of silicon, aluminum, calcium and barium and comprises the following steps: a test sample is preprocessed through the acid dissolution method, microwave-digested, naturally cooled, and then placed into a volumetric flask with scales, diluted and shaken until well distributed, a solution with a standard working curve is prepared, an atomic emission spectrograph of inductance coupled plasmas is started and led to reach the measurement requirement, a plasma torch is lighted, the relative standard deviation of the plasma torch is led to be less than 5 percent, wavelength of each element is chosen, the working curves are plotted, and the content of each element is computed. The method combines the microwave digesting sample dissolution technique with the atomic emission spectrograph of inductance coupled plasmas, can simultaneously analyze nine elements including silicon, etc., is accurate and quick, has greatly reduced reagent usage, and reduces the environmental pollution that is caused by chemical reagents.

Provided are a kit and method for quantitatively detecting trimethylamine N-oxide. The method for quantitatively detecting trimethylamine N-oxide comprise the following steps of adding trimethylamine N-oxide in an aqueous solution of human serum albumin to prepare a series of trimethylamine N-oxide standard solutions with a concentration gradient, adding an internal standard substance and a precipitating agent to each standard solution respectively, centrifuging the obtained standard solutions, obtaining a series of supernatant, analyzing the supernatant by using liquid chromatography and mass spectrometry respectively, and obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in each trimethylamine N-oxide standard solution and a relationship between the ratios and concentrations of trimethylamine N-oxide; adding the internal standard substance and the precipitating agent to a to-be-tested blood serum sample, conducting centrifugation, analyzing obtained supernatant by using liquid chromatography and mass spectrometry, obtaining the ratio of the chromatographic peak area of trimethylamine N-oxide to that of the internal standard substance in the to-be-tested blood serum sample, and obtaining the concentration of trimethylamine N-oxide in the to-be-tested blood serum sample according to the relationship between the ratios and the concentrations of trimethylamine N-oxide. According to the kit and method for quantitatively detecting trimethylamine N-oxide, the problem that unreliable detection results can be caused by application of water, a bovine serum albumin solution or a phosphate buffer solution and other blank biological matrix which are far from plasma components of the human body can be avoided, and the reliability of the detection results is improved.

A fast detection method of water microbe includes filtering water sample by microhole filtering film with different hole diameter, carrying out ATP luminous detection for used filtering film and for ATP standard solution and new microhole filtering film, calculating linear regression equation of Lg [ATP] = A+ BLg [CPM] based on logarithmic relation of ATP concentration to its luminous pulse counts and calculating out microbe content in water as well as ATP concentration in sample according CPM value of filtering film.

The invention relates to a method for measuring microelements in high titanium blast furnace slag, which comprises the following steps: firstly, adopting an alkali fusion method to pre-process samples, and leading the samples to be totally conversed into soluble salt, and preparing into sample solution, secondly, preparing standard solution of iron, manganese, vanadium and phosphor with different concentrations, thirdly, respectively measuring the spectral line strength of calcium in the standard solution of iron, manganese, vanadium and phosphor with different concentrations, utilizing the concentration of elements to be the abscissa and the spectral line strength to be the longitudinal coordinate to draw a working curve, fourthly, measuring the intensity of the spectral lines of iron, manganese, vanadium and phosphor in sample solution under the condition which is the same to the step three, and calculating and assuring the content of the four elements in each sample solution according to the working curve. The invention has the advantages that the method is simple and fast, which has little dosage of agents and samples, and can simultaneously measure a plurality of elements. The method shortens analysis time, and lowers test cost.

The invention discloses a method for determining content of potassium, sodium, calcium and magnesium in cigarette paper simultaneously, which includes the following steps: cutting 100+/-0.1 mg of a cigarette paper sample, using 100mL of 0.02mol/L hydrochloric acid as an extracting agent, ultrasonic-extracting for 18-22min to obtain extract liquor, selecting 5.0mL of the extract liquor to a 25mL volumetric flask, bringing to the volume with 0.02mol/L hydrochloric acid to obtain diluent, and filtering the diluent through a 0.45 mu m filtering film to obtain sample liquid to be detected; selecting sodium chloride, potassium chloride, hexahydrate magnesium chloride and anhydrous calcium chloride, using pure water as solvent, preparing mixed standard solution through gradual diluting, and adding 0.02mol/L hydrochloric acid to prepare series standard working solution; detecting and analyzing the series working solution and the sample liquid to be detected respectively through an ion chromatographic instrument; and drawing a standard curve and calculating results. The method is fast and simple to operate, high in sensitivity, low in detection limit, good in recovery rate, and suitable for batch analyzing of important inorganic elements in the cigarette paper.

A method to detect the acute biology toxicity by the photobacterium is to prepare the new photobacterium culture firstly, then to adjust the pH of tested water: the pH is 4.5 when the Cu density is higher than other metal and organic matter; the pH is 5.4 when the water sample does not contain Cu and other metal density is higher than the organic matter; the pH is 7.0 when the organic matter in water is higher than metal density. The ZnSO4 is as reference toxicity and matching a series of standard solution which are set in tube which is added in the photobacterium culture and detect the relative luminance. The luminance change of test water is expressed by the relative luminance. The computing formula is: the sample relative luminance = sample tube luminance/reference tube luminance. The invention uses the photobacterium not the toxicity to detect the acute biology toxicity of water.

The invention provides a method for determining calcium oxide and magnesium oxide in iron slag grains. The determination method comprises the following steps: weighing a sample; heating and dissolving by using acid; adding perchloric acid until the perchloric acid is fumed at a wet salt state; cooling, adding a 20% hexamethylenetetramine solution to adjust the pH (Potential of Hydrogen) value to 7-8, and preserving the temperature; adding a 1% copper reagent, diluting by water and filtering, wherein a filtering solution is a solution to be detected; separately taking the filtering solution, and mixing with the water; adding 1:1 triethanolamine and adjusting the pH to be more than 12 by using 20% potassium hydroxide; adding a calcium carboxylic acid indication agent; titrating by using an EDTA (Ethylene Diamine Tetraacetic Acid) standard solution and calculating the mass percent of the calcium oxide according to a formula shown in the specification; separately taking the filtering solution and mixing with the water in a ratio of 1:1, wherein the amount of the filtering solution is the same as that in the step 4; adding the 1:1 triethanolamine and adding an ammonia buffering solution with the pH of 10; adding a 1% chromium black T indication agent and titrating by using the EDTA standard solution; and calculating the mass percent of the magnesium oxide according to a formula shown in the specification. The method is short in flow and high in speed, and the aims of simplicity, rapidness and accuracy are realized.