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Method of detecting primer extension reaction, method of discriminating base type, device for discriminating base type, device for detecting pyrophosphate, method of detecting nucleic acid and tip for introducing sample solution

Convenient techniques for discriminating the base type in a base sequence of a nucleic acid are provided. The technique includes the step (a) of preparing a sample solution containing a nucleic acid, a primer having a base sequence that includes a complementary binding region which complementarily binds to the nucleic acid, and a nucleotide; the step (b) of allowing the sample solution to stand under a condition to cause an extension reaction of the primer, and producing pyrophosphate when the extension reaction is caused; the step (c) of bringing the sample solution into contact with the front face of a H+ hardly permeable membrane having H+-pyrophosphatase, which penetrates from front to back of the membrane, of which active site that hydrolyzes pyrophosphate being exposed to the front face; the step (d) of measuring the H+ concentration of at least either one of the solution at the front face side of the H+ hardly permeable membrane or the solution at the back face side of the H+ hardly permeable membrane, in a state where the H+-pyrophosphatase is immersed in the solution; the step (e) of detecting the extension reaction on the basis of the result of measurement in the step (d) ; and the step (f) of discriminating the base type in the base sequence of the nucleic acid on the basis of the result of detection in the step (e).
Owner:PANASONIC CORP

Binocular visible light camera and thermal infrared camera-based target identification method

The invention discloses a binocular visible light camera and thermal infrared camera-based target identification method. The method comprises the steps of calibrating internal and external parametersof two cameras of a binocular visible light camera through a position relationship between an image collected by the binocular visible light camera and a pseudo-random array stereoscopic target in a world coordinate system, and obtaining a rotation and translation matrix position relationship, between world coordinate systems, of the two cameras; according to an image collected by a thermal infrared camera, calibrating internal and external parameters of the thermal infrared camera; calibrating a position relationship between the binocular visible light camera and the thermal infrared camera;performing binocular stereoscopic visual matching on the images collected by the two cameras of the binocular visible light camera by adopting a sift feature detection algorithm, and calculating a visible light binocular three-dimensional point cloud according to a matching result; performing information fusion on temperature information of the thermal infrared camera and the three-dimensional point cloud of the binocular visible light camera; and inputting an information fusion result to a trained deep neural network for performing target identification.
Owner:SOUTHWEAT UNIV OF SCI & TECH
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