The invention relates to the technical field of
microfluidics and digital PCR detection, and discloses a
microfluidic chip for centrifugal droplet generation and a digital PCR detection method.The
microfluidic chip comprises a sealed cavity formed by a bottom plate and a cover plate, a through hole is formed in the center of the
chip and used for being fixed to centrifugal equipment, the core structure of the
chip is a droplet generation component, and the droplet generation component is arranged in the cavity. Comprising a sample adding cavity, a micro-
nozzle array and a
liquid drop collecting cavity, a sample is driven by
centrifugal force to generate nano-
liter-scale'water-in-oil 'liquid drops through micro-nozzles, the
chip is used for digital PCR reaction, each
liquid drop serves as an independent reaction unit to improve detection sensitivity and precision, the chip is suitable for detection of low-abundance
gene mutation, and by combining
Taqman-
MGB probe or other molecule detection technologies, the detection sensitivity and accuracy are improved. The chip can efficiently detect
mutation types of EGFR genes, including EGFR-T790M
mutation, has a wide clinical application prospect, provides a high-
throughput and high-precision droplet generation method, and is suitable for application scenarios of digital PCR,
single cell analysis and other microfluidic technologies.