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This invention relates to the field of molecular biology, and more particularly to a kit and method for detecting obesity gene polymorphisms. The invention develops a kit and nucleic acid mass spectrometry detection method for simultaneously detecting obesity gene polymorphisms rs1421085, rs9939609, rs1801282, rs1042713, and rs17782313. This allows for the rapid and simultaneous detection of ten genotypes at five polymorphic loci, making genetic testing for obesity-related polymorphisms more convenient and offering high sample throughput. This provides better technical support for obesity gene detection and helps to provide better suggestions for personal weight loss plans, demonstrating broad application prospects.