4results about How to "High sample throughput" patented technology

The embodiment of the invention discloses a primer group and application. Specific primers are designed for a UreA gene, a GyrA gene, a 23SrRNA gene, a PbP1 gene, a 16SrRNA gene, a porD gene, a CagA gene, a vacA gene, a CYP2C19 * 2 gene, a CYP2C19 * 3 gene, a CYP2C19 * 17 gene and mutation sites on the basis of a nucleic acid mass spectrometry technology, the helicobacter pylori detection kit has the advantages of high sensitivity and high accuracy, and helicobacter pylori identification, antibiotic sensitivity / drug resistance detection and virulence gene typing can be quickly and efficiently carried out; the method for detecting the metabolic type of the proton pump inhibitor has a good application prospect.

This invention relates to the field of molecular biology, and more particularly to a kit and method for detecting obesity gene polymorphisms. The invention develops a kit and nucleic acid mass spectrometry detection method for simultaneously detecting obesity gene polymorphisms rs1421085, rs9939609, rs1801282, rs1042713, and rs17782313. This allows for the rapid and simultaneous detection of ten genotypes at five polymorphic loci, making genetic testing for obesity-related polymorphisms more convenient and offering high sample throughput. This provides better technical support for obesity gene detection and helps to provide better suggestions for personal weight loss plans, demonstrating broad application prospects.

The invention discloses an iron nutrition deficiency or overload risk assessment method for gestational diabetes patients, and relates to molecular biology. The primer group for evaluating the iron nutrition deficiency or overload risk of the gestational diabetes mellitus patient is provided, the primer group is used for amplifying the mononucleotide polymorphic site of the hepcidin coding gene, and the primer length is 18-30 bp. And the single nucleotide polymorphic sites comprise rs10416533, rs7251432, rs8101606, rs55863037 and rs2293689, and the single nucleotide polymorphic sites comprise rs10416533, rs7251432, rs8101606, rs55863037, the primer group is as shown in SEQ ID NO: 1-15. The invention provides a detection kit comprising the primer group and a method for evaluating iron nutrition deficiency or overload risk of gestational diabetes mellitus patients by adopting the detection kit. The method has the advantages of ultrahigh flexibility, full-automatic data analysis, high PCR multiple number and high conversion success rate. The insertion and deletion INDEL and translocation fusion genes can be effectively detected, and the accuracy rate gt is high; the yield is 99.7%. The sample flux and the cost performance are high.

The invention relates to the technical field of biology, in particular to a primer group and application thereof in preparation of a thalassemia detection kit. The primer group is designed and obtained on the basis of at least one of sequences 1)-13): 1) chr16: 219267-219290; (2) chr16: 228804 to 228829, and the molecular weight of chr16: (3) chr16: 215255 to 215276; (4) chr16: 235945 to 235971, and chr16: 235945 to And (5) chr16: 196488 to 196508, and the molecular weight of chr16: 196488 (6) chr < 11 >: 5276986 to 5277008; (7) chr11: 5257290-5257314, and (7) chr11: 5257290- (8) chr < 11 >: 5249915 to 5249939; (9) chr11: 5258978 to 5259000, and (9) chr11: 5258978 (10) chr11: 5221888-5221912, (10) chr11: (5221888- (11) chr11: 5189975-5189996, (11) chr11: (5189975- (12) chr11: 5152341-5152365, (12) chr11: ( (11, 13) chr11: 5133905-5133925, (11, 12, 13) chr11 Wherein the primer is at least partially complementary to the sequences 1)-13).