35 results about "Deamination" patented technology

This utility model discloses a ternary precursor material mother liquor ammonia removal treatment system, including an ammonia removal tower, a falling film evaporator, a dilute ammonia water tank, a condenser, and an ammonia water storage tank. The top of the ammonia removal tower is connected to the shell side of the falling film evaporator section via an ammonia gas outlet pipe, and the bottom of the ammonia removal tower is connected to the gas-liquid separation section via a liquid outlet pipe. The shell side of the falling film evaporator section is connected to the cooling inlet end of the condenser via an ammonia gas outlet pipe, and the cooling outlet end of the condenser is connected to the ammonia water storage tank. A dilute ammonia water inlet pipe and a reflux pipe are connected between the shell side of the falling film evaporator section and the top of the dilute ammonia water tank. A liquid outlet pipe is connected to the gas-liquid separation section. A reflux pipe and a falling film circulation pump are connected between the liquid outlet pipe and the top of the tube side of the falling film evaporator section. A vapor compression unit is provided between the gas-liquid separation section and the ammonia removal tower. This utility model has the advantages of reducing the amount of vapor used in the ammonia removal tower, reducing energy consumption, achieving good ammonia removal effect from the mother liquor, achieving good ammonia water recovery effect, low system operating cost, and stable and reliable operation.

This invention discloses a method for the deamination functionalization of aliphatic nitrosamines, comprising: reacting an aliphatic nitrosamine as shown in formula (II) with a nucleophile in the presence of an acid and a solvent to generate a deamination functionalized product as shown in formula (III); wherein, R 1 R 2 R 3 Each group is independently selected from hydrogen, hydroxyl, alkyl, alkoxy, alkanoyl, alkoxycarbonyl, alkylsulfinyl, alkylsulfonyl, aryl, heteroaryl, phenylalkyl, or R 1 R 2 R 3 Two or three of the functional groups form a non-aromatic ring, cage-like compound; FG is a functional group in which a nucleophile participates in the reaction. The method of this invention has the advantages of inexpensive and readily available raw materials, simple reaction conditions, and wide substrate applicability.

The application discloses a method for recovering terephthalic acid from polyester-containing products, which comprises the following steps: (a) performing an enzymatic reaction by contacting the polyester-containing products with a degradation enzyme, using ammonia water / ammonia gas / liquid ammonia / ammonium hydroxide as a pH regulator in the enzymatic reaction, and forming a degradation solution containing ammonium terephthalate and a dihydric alcohol after the enzymatic reaction; (c) performing a heating treatment on the degradation solution; and (d) obtaining solid terephthalic acid through solid-liquid separation. Through the cycle mode of water dissolution-reconcentration-again deamination, the total recovery rate of PTA is significantly improved to more than 90%, efficient utilization of materials is realized, and the whole process still does not need to add acid and alkali, no waste salt is generated, and the process is green and economical.

A high pressure melamine production process wherein treating crude melamine comprises separating solid melamine from a mother liquor (1) comprising water, ammonia, carbon dioxide, residual melamine and OAT; the treatment of the mother liquor comprises deamination (100) and treatment of an ammonia depletion solution (4) in an OAT decomposition section (200); a purification treatment (300) of the OAT-depleted stream (6) produces a vapor stream (7) containing ammonia, CO2 and water and a first liquid stream (8) and a second liquid stream (9); said first liquid stream (8) comprising residual ammonia and having a CO2 concentration not greater than a target concentration; the second liquid stream (9) has an ammonia concentration not greater than a target concentration; at least part of the first liquid stream and / or at least part of the second liquid stream is recycled to the melamine production process.

The present application relates to the technical field of chemical separation, and discloses a methylamine separation and purification system and method, which can efficiently destroy hydrogen bond association system by accurately controlling flash evaporation pressure, temperature and material residence time, can recover the extraction water after removing trace organic impurities, can recover the extraction agent polarity and selectivity, can guarantee the stable separation efficiency of trimethylamine, can cut off the cross-unit pollution path from the source, can slow down the tower scaling and improve the mass transfer efficiency, can simultaneously match the whole process parameter monitoring system, can collect the operation parameters of each tower, product purity, impurity removal rate and hydrogen bond dissociation rate, can calculate the system running cycle and failure probability based on the data, and can propose the product purity stability and system energy consumption optimization strategy, so as to dynamically control the operation parameters of the whole link such as deamination, extraction, separation and recovery, and guarantee the stable output of high-purity methylamine product and the long-period continuous operation of the system.

The application belongs to the technical field of organic synthesis and specifically relates to a preparation process of high-efficiency taurine. The process specifically comprises the following steps: S1, raw material solution preparation and mother liquor threshold reuse; S2, construction of an aminolysis selective chemical environment; S3, aminolysis reaction; S4, deamination and clarification filtration; S5, segmented acidification-crystallization coupling; S6, crystal throwing and controlled cooling crystallization; S7, solid-liquid separation, washing and drying; and S8, mother liquor threshold detection and closed-loop reuse. The application constructs an aminolysis selective control, acidification-crystallization purification and mother liquor threshold reuse synergistic closed-loop process, stably controls a reaction system, dynamically determines an end point, and inhibits by-product generation from the source; segmented acidification and temperature-controlled crystallization are adopted to improve the purity of crystals and the first-time pharmaceutical-grade qualified rate; and the mother liquor is recycled according to a multi-index threshold access, thereby blocking impurity accumulation.

The invention belongs to the field of biological medicine, and particularly relates to a 12-bit deamination esterification impurity of terlipressin for injection and application. The deamination and esterification impurity is a terlipressin 12-site deamination and esterification compound generated by reaction of terlipressin 12-site amination glycine and mannitol, the molecular weight of a single isotope is 1391.5510, and the structural formula of the deamination and esterification impurity is shown as a formula I. The deamination and esterification impurity is a terlipressin 12-site deamination and esterification compound.

The invention relates to the technical field of wastewater treatment, and particularly discloses a biological deamination treatment process based on an iron-carbon coupling method. The invention discloses a biological deamination treatment process based on an iron-carbon coupling method. The biological deamination treatment process comprises the following steps: pretreatment: filling a reactor with iron-carbon composite filler with a sandwich structure; then circularly contacting the surface of the filler with an iron reducing bacteria solution and a sulfur autotrophic bacteria solution, and culturing and biofilm culturing to form a composite membrane; wherein the iron-carbon composite filler with the sandwich structure comprises two conductive net layers and an iron powder layer arranged between the two net layers; wastewater treatment: adjusting the pH and temperature of the wastewater, then introducing the wastewater into a reactor, and using hydrogen generated by iron-carbon reaction as a signal source: when the hydrogen concentration is greater than 0.5%, automatically closing aeration to enter a denitrification mode, and when the hydrogen concentration is less than 0.2%, starting aeration to convert into a nitrification mode; the composite membrane formed in the reactor continuously degrades ammonia nitrogen, and deamination treatment is completed.

This invention discloses a system and method for defluorination and ammonia removal from glass engraving wastewater. The defluorination and ammonia removal method includes the following steps: reacting glass engraving wastewater with an alkaline calcium-containing compound solution to produce a primary mixed liquid; reacting the primary mixed liquid with a fly ash suspension to obtain a secondary mixed liquid; subjecting the secondary mixed liquid to a flocculation reaction to produce a tertiary mixed liquid; filtering the tertiary mixed liquid to produce filtrate, neutralizing the filtrate with an oxalic acid solution, and then sequentially performing coagulation and flocculation reactions to produce a quaternary mixed liquid; subjecting the quaternary mixed liquid to sedimentation separation to produce a supernatant; and subjecting the supernatant to short-cut nitrification and denitrification treatment to produce a defluorinated and deammoniated supernatant. This invention uses fly ash to replace part of the slaked lime for defluorination, separates sludge and water through plate and frame filtration, adjusts the pH with oxalic acid, and adds coagulants and flocculants to achieve decalcification and hardening. The remaining oxalic acid provides a carbon source, and short-cut nitrification and denitrification are used for nitrogen removal, improving the defluorination and ammonia removal efficiency and reducing treatment costs.

This invention relates to the use of a class of deaminases or transaminases and their cofactors or metabolic substrates as drugs for the treatment of polyglutamine diseases. Specifically, this invention discovers that a series of transaminases and deaminases (such as asparagine synthase ASNS) can degrade polyglutamine aggregates by deaminating polyglutamine (polyQ) proteins, thereby treating polyglutamine diseases.

The invention belongs to the field of biological medicine, and particularly relates to an impurity detection method of a terlipressin preparation for injection. The impurity is the deamination esterification impurity of terlipressin and mannitol found for the first time, the structure of the impurity is shown as a formula I and / or a formula II, and the synthesized impurity is used as a reference substance and is detected through ultra-high performance liquid chromatography.

The application relates to the field of medicine synthesis, in particular to a three-step preparation method of a ketoprofen general compound. The ketoprofen general compound is prepared through three-step reactions of deamination, deesterification and acid hydrolysis from a prepared compound of formula IV, wherein R1 is -CONR4R5, -COX1, -COOR2 or -CN at the ortho position or the para position of an amine group, R2, R3, R4 and R5 are the same or different and are H or C1-C6 alkyl, and X1 is F, Cl, Br or I. The reaction is suitable for industrial production.

The invention discloses anti-vomiting hydrolyzed whey protein and a preparation method thereof, and belongs to the technical field of food processing. The product provided by the invention reduces the digestion burden of protein and relieves the symptom of vomiting. According to the method disclosed by the invention, protein sequences are mutually crosslinked by virtue of a treatment mode of first fibration, second polymerization and final hydrolysis, and a hydrolyzed polypeptide sequence is changed, so that the abundance of the polypeptide SLAMAASDISLA in a hydrolysate is improved. The polypeptide has the characteristics of being easy to recognize by intestinal peptidase and relatively high in PepT1 affinity, the bioavailability of protein is improved, and the deamination proportion of unutilized amino acid is reduced, so that substances generating nitrogen-containing toxoid are reduced, the stimulation of toxin accumulation on gastrointestinal mucosa is relieved, and the symptoms of nausea, vomiting and the like of a user are avoided.

The invention belongs to the technical field of carbon capture, and particularly relates to a high-efficiency low-energy-consumption carbon capture absorbent and a preparation method thereof. The preparation method comprises the following steps: protecting aminopyridine by using di-tert-butyl dicarbonate to obtain protected aminopyridine, then carrying out Mannich reaction on the protected aminopyridine, 2, 2, 3, 3, 4, 4, 5, 5-octafluorovaleraldehyde and 3-aminopropyltrimethoxysilane under the catalysis of acetic acid, and removing amino protection to generate an active agent; and grafting the active agent with the carrier through a silane chain segment to obtain the high-efficiency low-energy-consumption carbon capture absorbent. According to the invention, the adsorption capacity and the regeneration efficiency are synchronously improved, and the stability and the water vapor interference resistance of the adsorbent are also enhanced.

The invention discloses a sea snake tail glycosaminoglycan as well as a derivative, a pharmaceutically acceptable salt, a preparation method and an application of the sea snake tail glycosaminoglycan. The sea snake tail glycosaminoglycan has a structure as shown in a formula (I), is prepared by extracting, separating and purifying the sea snake tail, and is further subjected to deacetylation, carboxyl reduction, peroxidation depolymerization, deacylation and deamination depolymerization and gel column chromatography to obtain various derivatives of the sea snake tail glycosaminoglycan. The glycosaminoglycan compound and the derivative thereof show remarkable anticoagulation and antithrombotic activity, are low in bleeding risk, mainly act on an endogenous blood coagulation factor X enzyme complex (FXase) and a heparin cofactor II (HCII), and can provide a new thought for preparing medicines or functional foods for treating and / or preventing thrombotic diseases.

The invention relates to the technical field of organic synthetic chemistry, and particularly discloses a synthesis method of o-chlorobenzylamine. According to the method, o-chlorobenzyl chloride, liquid ammonia and caustic soda liquid are used as raw materials, and the preparation process mainly comprises the steps of amination reaction, liquid ammonia recovery, alkali treatment deamination and product purification. Specifically, in a high-pressure reaction kettle, liquid ammonia is used as a reaction medium and reacts with o-chlorobenzyl chloride under the conditions of low temperature and specific pressure, and after the reaction, the liquid ammonia is evaporated out and recycled for recycling; dissolving with water, adding alkali for dissociation, and removing residual ammonia gas under negative pressure; and finally, separating an organic phase by standing and layering, and dehydrating and filtering to obtain a high-purity o-chlorobenzylamine product. According to the method, by constructing a solvent-free system and cooperatively controlling the reaction temperature, the material proportion and post-treatment parameters, side reactions are remarkably inhibited, and therefore high-selectivity synthesis is achieved. The whole process has the advantages of simplicity and convenience in operation, environmental friendliness, high resource utilization rate and easiness in industrial implementation.

This invention relates to the field of biotechnology, and discloses an engineered *Yarrowia lipolytica* strain that produces p-coumaric acid using glucose as a substrate, its construction method, and its applications. Construction method: A tyrosine ammonia-lyase gene is integrated using a CRISPR / Cas9 localization and integration method. TAL In *Yarrowia lipophila* strains, the DAHP synthase gene was enhanced. ARO4 , DHS1 and AROG Overexpression of tyrosine synthase TYR Genes and histidine phosphotransferases HIS5 The protease gene was further integrated to synthesize exogenous phenylalanine deamination and hydroxylation pathway genes for p-coumaric acid, including phenylalanine ammonia-lyase gene, cinnamate hydroxylase gene, and P450 reductase gene. The p-coumaric acid produced by the engineered *Yarrowia lipolytica* strain of this invention can reach a maximum yield of 1.7 g / L in shake flasks, and a yield of 30 g / L in a 5 L fed-batch fermentation tank, demonstrating significant industrial application value.

This invention discloses a stability-improved complete thyroid hormone antigen, its preparation, and its application. The free amino group of the thyroid hormone or its deiodinated derivative moiety of this complete antigen is protected by a tert-butoxycarbonyl (Boc) group, and its carboxyl group is coupled to a carrier protein via an amide bond. The preparation method includes: protecting the amino group of a thyroid hormone molecule (such as T4, T3, etc.) with Boc2O, followed by activating its carboxyl group using the EDC / NHS method and coupling it to a carrier protein. This invention effectively overcomes the chemical instability problems of traditional complete antigens, such as easy photo-oxidative deiodination, easy racemization, and easy deamination reactions. In vitro detection reagents coated with the complete antigen of this invention exhibit longer shelf life, lower batch-to-batch variability, and extremely stable detection performance.

The application provides a method for synthesizing elacridar, comprising the following steps: S1), coupling reaction of compound 11 and compound 16 to obtain compound 12; S2), removing a deamination protection group from compound 12 to obtain compound 13; S3), obtaining compound 14 from compound 13; S4), reducing compound 14 to obtain compound 15 elacridar; wherein R1 represents a hydroxyl protection group, R2 represents an amino protection group, and X represents halogen. The method has the advantages of novel route, mild reaction condition, convenient post-treatment, low cost, high yield and suitability for industrial production. The reaction formula is as follows:

The invention belongs to the field of reproductive medicine, and particularly relates to application of deaminotyrosine in preparation of a reagent or a medicine for improving the quality of aged oocytes. The invention discloses and provides a mechanism for activating an ERK signal channel by utilizing deaminotyrosine specificity to act on the quality improvement of the aged oocytes for the first time. Experiments prove that the deaminotyrosine can reduce the abnormal form proportion of spindle bodies of oocytes, regulate the transformation of mitochondria to uniform distribution, improve the membrane potential of the mitochondria, reduce the ROS level, improve the maturation rate of the oocytes and improve the development rate of in-vitro fertilized blastocysts. The deaminated tyrosine is an endogenous and symbiotic microbial metabolite of a human body, so that the deaminated tyrosine has extremely high biological safety while improving the quality of aged oocytes, and an effective and safe new means is provided for improving the fertility potential of elderly women and optimizing and assisting a reproductive in-vitro maturation technology.

The invention relates to the technical field of biology, in particular to a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine. The invention discloses a preparation and purification method of 2 '-fluoro-2'-deoxyguanosine.The preparation method comprises the following steps that 2, 6-diaminopurine and 2 '-fluoro-2'-deoxyuridine generate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside under the catalysis of thymine nucleoside phosphorylase and purine nucleoside phosphorylase, filtering is conducted, and the 2 '-fluoro-2, 6-diaminopine-2'-deoxyguanosine is obtained. Then adenosine deaminase is added for catalysis, and 2 '-fluoro-2'-deoxyguanosine is generated; biosynthesis of the 2 '-fluoro-2'-deoxyguanosine is divided into two stages, the intermediate 2 '-fluoro-2, 6-diaminopine-2'-deoxynucleoside is synthesized firstly, and then deamination is performed to form the 2 '-fluoro-2'-deoxyguanosine, so that the problem that the conversion rate is extremely low when guanine with low solubility is taken as a substrate is solved.

The invention discloses a preparation method of a 2 '-modified inosine monomer, and belongs to the technical field of synthesis of medical intermediates. The method comprises the following steps: by taking a compound I as a starting raw material, preparing a compound II under the action of adenosine deaminase, then reacting the compound II with DMTrCl to generate a compound III, purifying the compound III, and reacting with a phosphorus reagent to prepare a compound IV. According to the method, adenosine deaminase is used as a deamination catalytic enzyme, an adenosine derivative is converted into an inosine derivative, the reaction can be carried out in pure water, the reaction is fast, the condition is mild, almost no by-product is generated, after the reaction is finished, a solvent is used for crystal transformation, a compound II with the purity of 99% + can be obtained, DMTr is added to the compound II, a compound III is formed, the compound III does not need column chromatography, and the yield is high. And then further reacting with a phosphorus reagent to obtain the 2 '-modified inosine monomer derivative with the purity of 99% +.

The invention relates to a water circulation treatment method and system for aquaculture, and belongs to the technical field of aquaculture water treatment.The method comprises the following steps that firstly, solid filtration, protein separation and short-cut nitrification treatment are conducted on effluent of a culture pond, and the molar concentration ratio of nitrite to ammonia nitrogen in the effluent is controlled to be (1.1-1.5): 1; then, 10-30% of water flow is shunted from the nitrified water body, anaerobic ammonia oxidation reaction is performed after deoxidation treatment, and ammonia nitrogen and nitrite are directly converted into nitrogen under the anaerobic condition; and finally, combining the deaminated side flow with the main flow water body, sterilizing and oxygenating, and then refluxing to the culture pond. According to the present invention, with the combination of the side flow deamination and the short-cut nitrification process, the complete removal of ammonia nitrogen is achieved, the accumulation of nitrate in the system is avoided, the dependence on the carbon source is reduced, the treatment process is simplified, and the cyclic utilization efficiency of the water resource is significantly improved.

This invention discloses a method for synthesizing an intermediate of isopropylpyridine, using 3-bromo-4-isopropylaniline as the starting material, and obtaining 2-bromo-4-chloroisopropylbenzene through deamination and chlorination under the action of nitric acid, triphosgene, and a base; wherein the base is 4-pyrrolylpyridine, 4-dimethylaminopyridine, pyridine, etc. The synthesis method of this invention uses inexpensive and readily available starting materials and reagents, resulting in low production costs. Furthermore, the reaction operation is simple, safe, and exhibits good selectivity with few side reactions. In particular, by optimizing the type of base, a reaction yield of at least 80%, or even 90%, and a product purity of at least 90%, or even 95%, can be obtained, making it suitable for large-scale industrial production.

The application discloses a method for preparing caprolactam by taking lysine-containing fermentation liquor, lysine or lysine salt as raw materials, and belongs to the technical field of caprolactam synthesis. The application solves the problems of low yield, complex and harsh operation conditions, high preparation cost and inability to be applied in large-scale industrialization in the prior art. The application takes lysine-containing fermentation liquor, lysine or lysine salt as starting raw materials, synthesizes aminocaprolactam under high temperature and high pressure with or without sodium hydroxide, then uses chloramine as a deaminating reagent to react with the amino group of aminocaprolactam, so that the amino group is converted into a hydrazine, the hydrazine is oxidized into an azo compound by chloramine, and then caprolactam is obtained by denitrogenation under alkaline conditions. The preparation method has the advantages of high product yield, short reaction path, high reaction efficiency, simple operation, low production cost and the like, and is suitable for large-scale production of caprolactam.

The present invention relates to a use of a class of deaminases or transaminases and cofactors or metabolic substrates thereof as drugs for treating polyglutamine (polyQ) diseases. Specifically, in the present invention, it is found that a series of transaminases and deaminases (asparagine synthetase (ASNS), etc.) can degrade polyQ aggregates by means of deamination of polyQ proteins, so as to treat polyQ diseases.

The application provides a biological pretreatment method of rare earth ore mining wastewater. The pretreatment method uses a mixed bacterial flora meeting the requirements of bacteria genus obtained through domestication to activate and gradually expand culture; then the mixed bacterial flora is domesticated in different concentrations of wastewater to be treated to adapt to the treatment environment, and finally added into the wastewater to perform deamination treatment. The application uses the mixed bacterial flora to realize rapid denitrification of the rare earth ore mining wastewater through an anaerobic ammonia oxidation process, expands the conventional treatment method, and reduces the load and operation cost of the wastewater treatment system.

The invention provides a continuous production system and method of 2-methylimidazole. The system comprises a feeding unit, an amination reaction unit, a deamination unit, a cyclization reaction unit and a concentration and crystallization unit which are connected in sequence, wherein the feeding unit comprises an acetaldehyde continuous feeding device and an ammonia source continuous feeding device; the amination reaction unit comprises a plurality of sections of amination microtube reactors which are connected in series; the deamination unit comprises a deamination device; the cyclization reaction unit comprises a plurality of cyclization reaction kettles and a glyoxal feeding device. According to respective characteristics of the amination reaction and the cyclization reaction, continuous production is realized in a pipe-kettle combined manner, so that the problem of large storage amount of hazardous chemicals on a production site can be solved, major potential safety hazards caused by the amination dangerous reaction can be eliminated, separation of the two-step reaction can be realized, in addition, the deamination step is added, and the production cost is reduced. Interference of excessive raw materials in the first-step amination reaction on the second-step cyclization reaction can be eliminated, side reactions are reduced, the raw material conversion rate and the product yield are improved, and the product quality is good.

The invention provides a bifunctional deaminase with adenine and cytosine bidirectional deamination activity as well as a mutant, a screening method and application of the bifunctional deaminase. The amino acid sequence of the bifunctional deaminase comprises any one of the following sequences: (1) an amino acid sequence as shown in SEQ ID NO: 2; (2) an amino acid sequence which is derived from the amino acid sequence as shown in SEQ ID NO: 2 through substitution, deletion or addition of one or more amino acids and has adenine deamination activity and cytosine deamination activity; and (3) an amino acid sequence which has at least 80% identity with the amino acid sequence as shown in SEQ ID NO: 2 and has adenine deamination activity and cytosine deamination activity. The single-structural-domain bifunctional deaminase variant with high activity and balanced bidirectional deamination capacity is obtained through directed evolution, the editing activity of the variant is remarkably improved, bidirectional editing can be achieved only through a single polypeptide chain, and the bidirectional deamination capacity is balanced.