60 results about "Deamination" patented technology

The invention discloses a sulfur-oxidizing bacterium screening and enriching nutrient solution, a sulfur-oxidizing bacterium enriching method and a deodorizing method. The sulfur-oxidizing bacterium screening and enriching nutrient solution comprises 1.0 g.L <-1 > of K2HPO4, 0.5 g.L <-1 > of NaHCO3, 0.05 g.L <-1 > of NH4Cl, 0.225 g.L <-1 > of Na2S. 9H2O and 0.001 g.L <-1 > of trace elements, wherein the trace elements comprise 0.5 g.L <-1 > of EDTA (Ethylene Diamine Tetraacetic Acid), 0.01 g.L <-1 > of (NH4) 6Mo7O24. 4H2O, 0.04 g.L <-1 > of ZnSO4. 7H2O, 0.03 g.L <-1 > of MnCl2. 7H2O, 0.02 g.L <-1 > of CuSO4. 5H2O The deodorization performance of the municipal sewage treatment plant is improved through synchronous desulfurization and deamination of the acidophilic sulfur-oxidizing bacteria, and compared with a traditional deodorization process, the synchronous desulfurization and deamination process can enrich high-abundance sulfur-oxidizing bacteria in a short time and shows higher ammonia gas and hydrogen sulfide removal capacity.

The present invention relates to antisense oligonucleotides (AONs) that can mediate RNA editing by binding to a target RNA nucleic acid molecule, preferably an RNA transcript molecule, in a cell and recruiting an endogenous deaminating enzyme in the cell to deaminate a target adenosine in the target RNA molecule to an inosine. The target RNA molecule is a transcript molecule form the SLC10A1 gene that encodes the Na+ / Taurocholate Co-transporting Polypeptide (NTCP), and the target adenosine is the adenosine in the GAC codon coding for aspartic acid (D) at position 24 of the NTCP protein. The deamination of the adenosine changes the amino acid to a glycine (G). The RNA editing of the adenosine will result in a loss-of-function of the NTCP protein, which will result in lowered uptake of bile acids from the portal circulation into the liver, thereby lowering the risk of suffering from disorders related to bile accumulation in the liver.

This utility model discloses a ternary precursor material mother liquor ammonia removal treatment system, including an ammonia removal tower, a falling film evaporator, a dilute ammonia water tank, a condenser, and an ammonia water storage tank. The top of the ammonia removal tower is connected to the shell side of the falling film evaporator section via an ammonia gas outlet pipe, and the bottom of the ammonia removal tower is connected to the gas-liquid separation section via a liquid outlet pipe. The shell side of the falling film evaporator section is connected to the cooling inlet end of the condenser via an ammonia gas outlet pipe, and the cooling outlet end of the condenser is connected to the ammonia water storage tank. A dilute ammonia water inlet pipe and a reflux pipe are connected between the shell side of the falling film evaporator section and the top of the dilute ammonia water tank. A liquid outlet pipe is connected to the gas-liquid separation section. A reflux pipe and a falling film circulation pump are connected between the liquid outlet pipe and the top of the tube side of the falling film evaporator section. A vapor compression unit is provided between the gas-liquid separation section and the ammonia removal tower. This utility model has the advantages of reducing the amount of vapor used in the ammonia removal tower, reducing energy consumption, achieving good ammonia removal effect from the mother liquor, achieving good ammonia water recovery effect, low system operating cost, and stable and reliable operation.

This invention discloses a method for the deamination functionalization of aliphatic nitrosamines, comprising: reacting an aliphatic nitrosamine as shown in formula (II) with a nucleophile in the presence of an acid and a solvent to generate a deamination functionalized product as shown in formula (III); wherein, R 1 R 2 R 3 Each group is independently selected from hydrogen, hydroxyl, alkyl, alkoxy, alkanoyl, alkoxycarbonyl, alkylsulfinyl, alkylsulfonyl, aryl, heteroaryl, phenylalkyl, or R 1 R 2 R 3 Two or three of the functional groups form a non-aromatic ring, cage-like compound; FG is a functional group in which a nucleophile participates in the reaction. The method of this invention has the advantages of inexpensive and readily available raw materials, simple reaction conditions, and wide substrate applicability.

The application discloses a method for recovering terephthalic acid from polyester-containing products, which comprises the following steps: (a) performing an enzymatic reaction by contacting the polyester-containing products with a degradation enzyme, using ammonia water / ammonia gas / liquid ammonia / ammonium hydroxide as a pH regulator in the enzymatic reaction, and forming a degradation solution containing ammonium terephthalate and a dihydric alcohol after the enzymatic reaction; (c) performing a heating treatment on the degradation solution; and (d) obtaining solid terephthalic acid through solid-liquid separation. Through the cycle mode of water dissolution-reconcentration-again deamination, the total recovery rate of PTA is significantly improved to more than 90%, efficient utilization of materials is realized, and the whole process still does not need to add acid and alkali, no waste salt is generated, and the process is green and economical.

The present invention discloses a bio-based high-strength solvent-resistant hyperbranched adhesive and a preparation method thereof, belonging to the field of bonding technology. The present invention prepares a hyperbranched polymer containing rich amino functional groups by deamination polycondensation reaction, and crosslinks it with glycerol triglycidyl ether through addition to obtain the high-strength, solvent-resistant bio-based hyperbranched adhesive MDUG adhesive of the present invention. The adhesive prepared by the present invention is widely applicable to bonding various materials, has high bonding strength and good solvent resistance, and the bonded substrate can also maintain its stability in extremely cold or high temperature environments (-196°C to 100°C), and adopts a simple solvent-free synthesis method, is easy to operate, and has low cost.

The invention relates to the technical field of microorganism culture and screening, and discloses a culture medium, a preparation method thereof and application of the culture medium in screening mineralized microorganisms, and the culture medium contains peptone, yeast extract powder, sodium chloride, magnesium chloride and calcium chloride. The culture medium takes peptone and yeast extract powder as core components, is not only suitable for screening urealysis type mineralized bacteria, but also provides nutrient substances required by growth of amino acid deamination, carbonic anhydrase catalysis or heterotrophic metabolism driven type mineralized microorganisms, comprehensively screens and prevents potential mineralized bacteria from being omitted. The culture medium can be used for accurately and efficiently screening metal surface mineralizable microorganisms. According to the culture medium, the ecological adaptability of marine strains is optimized, peptone and yeast extract powder supply nutrient substances needed by growth of marine bacteria, sodium chloride, magnesium chloride and calcium chloride are mainly used for simulating the marine salinity environment and adjusting the osmotic pressure and enzyme activity of the bacteria, the matching degree with the environment is high, and the enrichment efficiency is high.

A high pressure melamine production process wherein treating crude melamine comprises separating solid melamine from a mother liquor (1) comprising water, ammonia, carbon dioxide, residual melamine and OAT; the treatment of the mother liquor comprises deamination (100) and treatment of an ammonia depletion solution (4) in an OAT decomposition section (200); a purification treatment (300) of the OAT-depleted stream (6) produces a vapor stream (7) containing ammonia, CO2 and water and a first liquid stream (8) and a second liquid stream (9); said first liquid stream (8) comprising residual ammonia and having a CO2 concentration not greater than a target concentration; the second liquid stream (9) has an ammonia concentration not greater than a target concentration; at least part of the first liquid stream and / or at least part of the second liquid stream is recycled to the melamine production process.

The present application relates to the technical field of chemical separation, and discloses a methylamine separation and purification system and method, which can efficiently destroy hydrogen bond association system by accurately controlling flash evaporation pressure, temperature and material residence time, can recover the extraction water after removing trace organic impurities, can recover the extraction agent polarity and selectivity, can guarantee the stable separation efficiency of trimethylamine, can cut off the cross-unit pollution path from the source, can slow down the tower scaling and improve the mass transfer efficiency, can simultaneously match the whole process parameter monitoring system, can collect the operation parameters of each tower, product purity, impurity removal rate and hydrogen bond dissociation rate, can calculate the system running cycle and failure probability based on the data, and can propose the product purity stability and system energy consumption optimization strategy, so as to dynamically control the operation parameters of the whole link such as deamination, extraction, separation and recovery, and guarantee the stable output of high-purity methylamine product and the long-period continuous operation of the system.

The application discloses a system and method for extracting ammonia from solvent regenerator overhead reflux to a deamination tower, and belongs to the technical field of solvent regeneration. The system comprises a solvent regenerator, a deamination tower, a reboiler, a water cooler, a first circulating pump, a second circulating pump and a static mixer. The pipeline connecting the first circulating pump and the water cooler is connected with the pipeline connecting the second circulating pump and the static mixer through an ammonia extraction pipeline. The ammonia collected at the top of the regenerator affects the desulfurization effect of the solvent. In the application, the ammonia water collected at the top of the regenerator is sent to the deamination tower for treatment, so that the ammonia nitrogen and hydrogen sulfide contents in the amine solution after regeneration are reduced, and the amine regeneration effect is ensured. The application of the solvent regenerator overhead reflux ammonia extraction to the deamination tower to a hydrogenation device for removing hydrogen sulfide in circulating hydrogen can solve the common problem of poor desulfurization effect, and can also be applied to other naphthenic base distillate hydrogenation devices of various tonnages.

The application belongs to the technical field of organic synthesis and specifically relates to a preparation process of high-efficiency taurine. The process specifically comprises the following steps: S1, raw material solution preparation and mother liquor threshold reuse; S2, construction of an aminolysis selective chemical environment; S3, aminolysis reaction; S4, deamination and clarification filtration; S5, segmented acidification-crystallization coupling; S6, crystal throwing and controlled cooling crystallization; S7, solid-liquid separation, washing and drying; and S8, mother liquor threshold detection and closed-loop reuse. The application constructs an aminolysis selective control, acidification-crystallization purification and mother liquor threshold reuse synergistic closed-loop process, stably controls a reaction system, dynamically determines an end point, and inhibits by-product generation from the source; segmented acidification and temperature-controlled crystallization are adopted to improve the purity of crystals and the first-time pharmaceutical-grade qualified rate; and the mother liquor is recycled according to a multi-index threshold access, thereby blocking impurity accumulation.

An emission reduction device for ammonia in granulation exhaust gas from a urea production device. The emission reduction device comprises a combined tower body (1), a deamination section (2) arranged at a lower portion inside the combined tower body (1), and an ammonia recovery section (3) arranged at an upper portion inside the combined tower body; and further comprises a partition plate (7) and a gas riser (8) that are arranged between the deamination section (2) and the ammonia recovery section (3), wherein the deamination section (2) and the ammonia recovery section (3) are in gas phase communication but not in liquid phase communication. The emission reduction device can be flexibly mounted in a granulation tower or a granulation plant, has low investment costs and operation costs, and has a prolonged service life.

The invention belongs to the field of biological medicine, and particularly relates to a 12-bit deamination esterification impurity of terlipressin for injection and application. The deamination and esterification impurity is a terlipressin 12-site deamination and esterification compound generated by reaction of terlipressin 12-site amination glycine and mannitol, the molecular weight of a single isotope is 1391.5510, and the structural formula of the deamination and esterification impurity is shown as a formula I. The deamination and esterification impurity is a terlipressin 12-site deamination and esterification compound.

The invention relates to the technical field of wastewater treatment, and particularly discloses a biological deamination treatment process based on an iron-carbon coupling method. The invention discloses a biological deamination treatment process based on an iron-carbon coupling method. The biological deamination treatment process comprises the following steps: pretreatment: filling a reactor with iron-carbon composite filler with a sandwich structure; then circularly contacting the surface of the filler with an iron reducing bacteria solution and a sulfur autotrophic bacteria solution, and culturing and biofilm culturing to form a composite membrane; wherein the iron-carbon composite filler with the sandwich structure comprises two conductive net layers and an iron powder layer arranged between the two net layers; wastewater treatment: adjusting the pH and temperature of the wastewater, then introducing the wastewater into a reactor, and using hydrogen generated by iron-carbon reaction as a signal source: when the hydrogen concentration is greater than 0.5%, automatically closing aeration to enter a denitrification mode, and when the hydrogen concentration is less than 0.2%, starting aeration to convert into a nitrification mode; the composite membrane formed in the reactor continuously degrades ammonia nitrogen, and deamination treatment is completed.

This invention discloses a system and method for defluorination and ammonia removal from glass engraving wastewater. The defluorination and ammonia removal method includes the following steps: reacting glass engraving wastewater with an alkaline calcium-containing compound solution to produce a primary mixed liquid; reacting the primary mixed liquid with a fly ash suspension to obtain a secondary mixed liquid; subjecting the secondary mixed liquid to a flocculation reaction to produce a tertiary mixed liquid; filtering the tertiary mixed liquid to produce filtrate, neutralizing the filtrate with an oxalic acid solution, and then sequentially performing coagulation and flocculation reactions to produce a quaternary mixed liquid; subjecting the quaternary mixed liquid to sedimentation separation to produce a supernatant; and subjecting the supernatant to short-cut nitrification and denitrification treatment to produce a defluorinated and deammoniated supernatant. This invention uses fly ash to replace part of the slaked lime for defluorination, separates sludge and water through plate and frame filtration, adjusts the pH with oxalic acid, and adds coagulants and flocculants to achieve decalcification and hardening. The remaining oxalic acid provides a carbon source, and short-cut nitrification and denitrification are used for nitrogen removal, improving the defluorination and ammonia removal efficiency and reducing treatment costs.

This invention relates to the use of a class of deaminases or transaminases and their cofactors or metabolic substrates as drugs for the treatment of polyglutamine diseases. Specifically, this invention discovers that a series of transaminases and deaminases (such as asparagine synthase ASNS) can degrade polyglutamine aggregates by deaminating polyglutamine (polyQ) proteins, thereby treating polyglutamine diseases.

The invention belongs to the field of biological medicine, and particularly relates to an impurity detection method of a terlipressin preparation for injection. The impurity is the deamination esterification impurity of terlipressin and mannitol found for the first time, the structure of the impurity is shown as a formula I and / or a formula II, and the synthesized impurity is used as a reference substance and is detected through ultra-high performance liquid chromatography.

The application relates to the field of medicine synthesis, in particular to a three-step preparation method of a ketoprofen general compound. The ketoprofen general compound is prepared through three-step reactions of deamination, deesterification and acid hydrolysis from a prepared compound of formula IV, wherein R1 is -CONR4R5, -COX1, -COOR2 or -CN at the ortho position or the para position of an amine group, R2, R3, R4 and R5 are the same or different and are H or C1-C6 alkyl, and X1 is F, Cl, Br or I. The reaction is suitable for industrial production.

The invention relates to the technical field of microorganisms and sewage treatment, in particular to a strain of European nitrosomonas and application thereof. The strain is European nitrosomonas DZ01, the strain is classified and named as Nitrosomonas europaea DZ01, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20241936. The European nitrosomonas DZ01 provided by the invention can tolerate a high-concentration ammonia nitrogen environment of 2000mg / L, achieves the optimal metabolic activity under the initial ammonia nitrogen concentration of 216mg / L, and realizes efficient deamination under a room temperature condition; according to the present invention, the method has characteristics of stable denitrification in the pretreated electroplating wastewater, and can maintain the deamination function in the electronic manufacturing industry wastewater containing 400 mg / L MAH, such that the stability of biological metabolism is ensured, and the ammonia nitrogen removal problem in the industrial wastewater is well solved.

Described herein are various methods of removing uracils due to the deamination of unmethylated cytosines in an assay using engineered cytosine deaminases to deaminate methylated cytosines. The Chemoenzymatic Uracil Replacement of Nucleobases (ChURN) method includes providing a sample comprising single stranded DNA library fragments in which a cytosine deaminase has deaminated methylated cytosines; contacting the sample with an uracil DNA glycosylase (UDG) to deglycosylate uracil residues to form abasic sites having a hemiacetal formation within the single stranded DNA library fragments; contacting the sample with a reactive cytosine nucleobase analog to install a cytosine at abasic sites thru a noncanonical linkage; and subjecting the sample to polymerase chain reaction (PCR) amplification resulting in double stranded DNA corrected library fragments. The Uracil Enzymatic Removal and Substitution at Errors (U-ERASE) method includes providing a sample comprising single stranded DNA library fragments in which a cytosine deaminase has deaminated methylated cytosines, synthesizing double stranded DNA library fragments in which the second strand is tagged to facilitate its downstream degradation, treating the double stranded DNA library fragments with an uracil DNA glycosylase and an endonuclease resulting in the removal of uracil bases and single nucleotide gaps at those sites, and repairing the single nucleotide gaps through treatment with a polymerase, dCTP, and a ligase, resulting in replacement of false positive uracil bases with a mismatched base, such as cytosine. Subsequently, the second strand is selectively degraded, allowing for selective amplification of the original DNA strand via PCR.

The invention discloses anti-vomiting hydrolyzed whey protein and a preparation method thereof, and belongs to the technical field of food processing. The product provided by the invention reduces the digestion burden of protein and relieves the symptom of vomiting. According to the method disclosed by the invention, protein sequences are mutually crosslinked by virtue of a treatment mode of first fibration, second polymerization and final hydrolysis, and a hydrolyzed polypeptide sequence is changed, so that the abundance of the polypeptide SLAMAASDISLA in a hydrolysate is improved. The polypeptide has the characteristics of being easy to recognize by intestinal peptidase and relatively high in PepT1 affinity, the bioavailability of protein is improved, and the deamination proportion of unutilized amino acid is reduced, so that substances generating nitrogen-containing toxoid are reduced, the stimulation of toxin accumulation on gastrointestinal mucosa is relieved, and the symptoms of nausea, vomiting and the like of a user are avoided.

The invention belongs to the technical field of carbon capture, and particularly relates to a high-efficiency low-energy-consumption carbon capture absorbent and a preparation method thereof. The preparation method comprises the following steps: protecting aminopyridine by using di-tert-butyl dicarbonate to obtain protected aminopyridine, then carrying out Mannich reaction on the protected aminopyridine, 2, 2, 3, 3, 4, 4, 5, 5-octafluorovaleraldehyde and 3-aminopropyltrimethoxysilane under the catalysis of acetic acid, and removing amino protection to generate an active agent; and grafting the active agent with the carrier through a silane chain segment to obtain the high-efficiency low-energy-consumption carbon capture absorbent. According to the invention, the adsorption capacity and the regeneration efficiency are synchronously improved, and the stability and the water vapor interference resistance of the adsorbent are also enhanced.

The invention belongs to the technical field of solid waste treatment, and particularly relates to a method for efficient deamination and cyclic utilization of ammonia-containing calcium slag. The method comprises the following steps: S1, preheating for deamination: preheating ammonia-containing calcium slag by using roasting flue gas to convert free ammonia from a liquid state into a gaseous state for removal so as to obtain deaminated calcium slag and ammonia-containing gas; s2, water-cooling absorption: cooling and condensing the ammonia-containing gas into ammonia water through a heat exchanger, and recycling the ammonia water for preparing a tungsten smelting desorption agent; s3, roasting conversion: roasting and converting the deaminated calcium slag into quick lime; s4, adjusting the pH value: adjusting the pH value of the ammonia-containing wastewater by using the quicklime prepared by conversion, and returning ammonia-containing calcium slag after filter pressing to the step S1 for circular treatment. According to the method, efficient removal and resource recovery of ammonia in the ammonia-containing calcium slag are achieved, meanwhile, the calcium slag is converted into quick lime, a closed-loop cyclic utilization path is constructed, the common problem that the treatment and utilization difficulty of the ammonia-containing calcium slag is large is solved, and the method has the advantages of being energy-saving, environmentally friendly, low in cost, easy and convenient to operate and the like.

The invention discloses a sea snake tail glycosaminoglycan as well as a derivative, a pharmaceutically acceptable salt, a preparation method and an application of the sea snake tail glycosaminoglycan. The sea snake tail glycosaminoglycan has a structure as shown in a formula (I), is prepared by extracting, separating and purifying the sea snake tail, and is further subjected to deacetylation, carboxyl reduction, peroxidation depolymerization, deacylation and deamination depolymerization and gel column chromatography to obtain various derivatives of the sea snake tail glycosaminoglycan. The glycosaminoglycan compound and the derivative thereof show remarkable anticoagulation and antithrombotic activity, are low in bleeding risk, mainly act on an endogenous blood coagulation factor X enzyme complex (FXase) and a heparin cofactor II (HCII), and can provide a new thought for preparing medicines or functional foods for treating and / or preventing thrombotic diseases.

The invention relates to the technical field of organic synthetic chemistry, and particularly discloses a synthesis method of o-chlorobenzylamine. According to the method, o-chlorobenzyl chloride, liquid ammonia and caustic soda liquid are used as raw materials, and the preparation process mainly comprises the steps of amination reaction, liquid ammonia recovery, alkali treatment deamination and product purification. Specifically, in a high-pressure reaction kettle, liquid ammonia is used as a reaction medium and reacts with o-chlorobenzyl chloride under the conditions of low temperature and specific pressure, and after the reaction, the liquid ammonia is evaporated out and recycled for recycling; dissolving with water, adding alkali for dissociation, and removing residual ammonia gas under negative pressure; and finally, separating an organic phase by standing and layering, and dehydrating and filtering to obtain a high-purity o-chlorobenzylamine product. According to the method, by constructing a solvent-free system and cooperatively controlling the reaction temperature, the material proportion and post-treatment parameters, side reactions are remarkably inhibited, and therefore high-selectivity synthesis is achieved. The whole process has the advantages of simplicity and convenience in operation, environmental friendliness, high resource utilization rate and easiness in industrial implementation.

The present invention relates to antisense oligonucleotides (AONs) that can mediate RNA editing by binding to a target RNA nucleic acid molecule, preferably an RNA transcript molecule, in a cell and recruiting an endogenous deaminating enzyme in the cell to deaminate a target adenosine in the target RNA molecule to an inosine. The target RNA molecule is a transcript molecule form the human PNPLA3 gene that encodes the I148M variant of the Patatin-Like Phospholipase domain-containing protein 3 (PNPLA3), and the target adenosine is the adenosine in the AUG codon coding for methionine (M) at position 148 of the PNPLA3 I148M variant protein. The deamination of the adenosine changes the amino acid to a valine (V). The RNA editing of the adenosine will result in a reversion of the functionality of the PNPLA3 protein that is similar or equal to the functionality of the wildtype PNPLA3 protein, which will result in lowered fattening of the liver, thereby lowering the risk of suffering from disorders related to non-alcoholic fatty liver disease (NAFLD) caused by the I148M variant.

The present disclosure relates to the field of diseases caused by iron overload, such as homeostatic iron regulatory protein (HFE) hemochromatosis. The present disclosure provides oligonucleotides for RNA editing technology that target and deaminate the c.845G>A nucleotide in the transcript of the p.Cys282Tyr (C282Y) mutant human HFE gene, reducing iron overload, particularly in the liver.

The invention discloses a preparation method of 3, 5-diaminobenzoic acid, which comprises the following steps: by taking parachlorobenzoic acid as a raw material, carrying out amination reaction, selective chlorination and diazotization deamination to obtain a 3, 5-dichlorobenzoic acid intermediate, and finally carrying out amination reaction to obtain the 3, 5-diaminobenzoic acid. The purity of the 3, 5-diaminobenzoic acid solid product obtained in the process is greater than 98%, and the total yield can reach more than 79%. The preparation method of the 3, 5-diaminobenzoic acid is specifically limited, traditional processes such as nitration and hydrogenation are not needed, and on the premise that the high yield is obtained, the whole steps are simple and safe, and industrial implementation is easy.

This invention relates to the field of biotechnology, and discloses an engineered *Yarrowia lipolytica* strain that produces p-coumaric acid using glucose as a substrate, its construction method, and its applications. Construction method: A tyrosine ammonia-lyase gene is integrated using a CRISPR / Cas9 localization and integration method. TAL In *Yarrowia lipophila* strains, the DAHP synthase gene was enhanced. ARO4 , DHS1 and AROG Overexpression of tyrosine synthase TYR Genes and histidine phosphotransferases HIS5 The protease gene was further integrated to synthesize exogenous phenylalanine deamination and hydroxylation pathway genes for p-coumaric acid, including phenylalanine ammonia-lyase gene, cinnamate hydroxylase gene, and P450 reductase gene. The p-coumaric acid produced by the engineered *Yarrowia lipolytica* strain of this invention can reach a maximum yield of 1.7 g / L in shake flasks, and a yield of 30 g / L in a 5 L fed-batch fermentation tank, demonstrating significant industrial application value.