11 results about "Pyrimidine Nucleotides" patented technology

The invention provides a uracil production strain and a construction method and application thereof.According to the strain, on an E.coli UR14 genome by means of a CRIPSR / Cas9 gene editing technology, firstly, psuG genes, preTA genes, rutA genes and upp genes are knocked out, so that decomposition of uracil is blocked; then, a uridine phosphorylase gene udp and a pyrimidine-5 '-nucleotide nucleotidase gene ppnN are subjected to overexpression, and synthesis and accumulation of uracil are synergistically enhanced; and finally, overexpression of the ribose phosphate mutase gene pgm further enhances the conversion of a by-product ribose phosphate 1-precursor 5-ribose phosphate 1-pyrophosphate and improves the carbon utilization rate, and the obtained strain has good genetic stability and high fermentation yield, can stably produce uracil, and has wide application prospects.

A fibrillin production promoter contains at least one pyrimidine nucleotide or precursor thereof as an active ingredient.

This invention relates to a pharmaceutical composition for tumor treatment and its application. Specifically, this invention relates to a composition containing a fluorouracil drug and pyrimidine nucleosides and their derivatives. Fluorouracil drugs and pyrimidine nucleotides and their derivatives have a synergistic antitumor effect; combined use can reduce the concentration of single drugs and achieve better antitumor efficacy, while reducing toxic side effects.

The present application belongs to the technical field of genetic engineering, and particularly relates to an engineered bacillus subtilis, a preparation method and application thereof. In the present application, in order to improve the supply of precursor cytidine triphosphate (CTP) in the process of fermentation of bacillus subtilis for producing cytidine, first, the feedback-inhibited CTP synthetase PyrG E156K is overexpressed to promote the conversion of uridine triphosphate to CTP; then, the repressor gene of the pyrimidine operon pyrR is knocked out to promote the synthesis of uridine monophosphate, and further promote the synthesis of CTP; finally, the pyrimidine nucleotide degradation genes pdp and cdd are knocked out to block the conversion of uridine / cytidine to uracil / cytosine and the conversion of cytidine to uridine, respectively, reduce the consumption of pyrimidine nucleotides, and further facilitate the intracellular accumulation of CTP. The yield of cytidine of the finally constructed engineering bacteria is about 12.6 times that of the original starting bacteria, and the results of the present application further prove the importance of CTP for cytidine synthesis.

The present invention is a muscular atrophy inhibitor comprising at least one pyrimidine nucleotide or a precursor thereof as an active ingredient. Also, the present invention is a method for inhibiting muscular atrophy by administrating at least one pyrimidine nucleotide or a precursor thereof.

Provided herein are methods, compositions, and kits related to using a CpG binding protein. In one embodiment, the present disclosure includes methods, compositions, and kits related to using a CpG binding protein with a cytidine deaminase protein to identify methylated cytosine nucleotides. The cytidine deaminase can be an altered cytidine deaminase that includes an amino acid substitution mutation at a position functionally equivalent to (Tyr / Phe)130 in a wild-type APOBEC3A protein. In another embodiment, the present disclosure includes methods, compositions, and kits related to using a CpG binding protein with a ten-eleven translocase (TET) protein to identify methylated cytosine nucleotides.

A collagen production promoter contains at least one pyrimidine nucleotide or precursor thereof as an active ingredient.

The invention relates to a novel enzyme composition comprising at least two different thermostable polypeptides having type II DNA methyltransferase activity as well as a restriction / modification system in particular for the transformation of microorganisms of the genus Caldicellulosiruptor, wherein said polypeptides methylate an adenine in a asymmetric DNA recognition site, where the adenine nucleotide is followed by a thymine nucleotide in the linear sequence of bases along its 5′→3′ direction, wherein the DNA recognition site is 5′-GCATC-3′ and / or wherein said polypeptide methylate the adenine in a complement DNA recognition site, where a thymine nucleotide is followed by a adenine nucleotide in the linear sequence of bases along its 3′→5′ direction, wherein the DNA recognition site is 3′-CGTAG-5′.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

The invention provides a high-temperature-resistant uracil nucleotide kinase mutant and application thereof, a coding gene of the mutant, and application of the mutant in preparing uridine triphosphate from uracil nucleotide through enzyme catalysis. The method for preparing uridine triphosphate from the high-temperature-resistant UMP kinase mutant has the advantages of high yield, simple process, short period, convenience in industrial production and the like. The UMP kinase and the mutant thereof can be efficiently expressed in escherichia coli, the optimum enzyme activity temperature of the mutant enzyme is 70 DEG C, and the mutant enzyme shows relatively high tolerance to a high-temperature environment of 50-70 DEG C. Under the conditions that the reaction temperature is 50 DEG C, the substrate concentration is 150 mM and acetic acid kinase is added as a coenzyme, the conversion rate of uridine nucleotide catalyzed by enzyme liquid obtained by carrying out heat treatment on the UMP kinase mutant at 70 DEG C reaches 96% within only 2 hours, so that a good technical support is provided for industrial large-scale production of uridine triphosphate.

The invention belongs to the technical field of gene engineering, and particularly relates to engineering modified bacillus subtilis, a preparation method and application thereof. In the invention, in order to improve the supply of precursor cytidine triphosphate (CTP) in the process of producing citicoline by fermentation of bacillus subtilis, firstly, CTP synthetase PyrGE156K with feedback inhibition removed is overexpressed, and the conversion from uridine triphosphate to CTP is promoted; then, a repressor protein gene pyrR of the pyrimidine operon is knocked out, synthesis of uridine monophosphate is promoted, and then synthesis of CTP is promoted; finally, the pyrimidine nucleotide degradation genes pdp and cdd are knocked out, conversion from uridine / cytidine to uracil / cytosine and conversion from cytidine to uridine are blocked respectively, consumption of pyrimidine nucleotide is reduced, and intracellular accumulation of CTP is facilitated. The citicoline yield of the finally constructed engineering bacterium is about 12.6 times of the yield of the original starting bacterium, and the result of the invention further proves the importance of CTP on citicoline synthesis.