7 results about "Pyrimidine Nucleotides" patented technology

The invention provides a uracil production strain and a construction method and application thereof.According to the strain, on an E.coli UR14 genome by means of a CRIPSR / Cas9 gene editing technology, firstly, psuG genes, preTA genes, rutA genes and upp genes are knocked out, so that decomposition of uracil is blocked; then, a uridine phosphorylase gene udp and a pyrimidine-5 '-nucleotide nucleotidase gene ppnN are subjected to overexpression, and synthesis and accumulation of uracil are synergistically enhanced; and finally, overexpression of the ribose phosphate mutase gene pgm further enhances the conversion of a by-product ribose phosphate 1-precursor 5-ribose phosphate 1-pyrophosphate and improves the carbon utilization rate, and the obtained strain has good genetic stability and high fermentation yield, can stably produce uracil, and has wide application prospects.

A fibrillin production promoter contains at least one pyrimidine nucleotide or precursor thereof as an active ingredient.

This invention relates to a pharmaceutical composition for tumor treatment and its application. Specifically, this invention relates to a composition containing a fluorouracil drug and pyrimidine nucleosides and their derivatives. Fluorouracil drugs and pyrimidine nucleotides and their derivatives have a synergistic antitumor effect; combined use can reduce the concentration of single drugs and achieve better antitumor efficacy, while reducing toxic side effects.

The present invention is a muscular atrophy inhibitor comprising at least one pyrimidine nucleotide or a precursor thereof as an active ingredient. Also, the present invention is a method for inhibiting muscular atrophy by administrating at least one pyrimidine nucleotide or a precursor thereof.

Provided herein are methods, compositions, and kits related to using a CpG binding protein. In one embodiment, the present disclosure includes methods, compositions, and kits related to using a CpG binding protein with a cytidine deaminase protein to identify methylated cytosine nucleotides. The cytidine deaminase can be an altered cytidine deaminase that includes an amino acid substitution mutation at a position functionally equivalent to (Tyr / Phe)130 in a wild-type APOBEC3A protein. In another embodiment, the present disclosure includes methods, compositions, and kits related to using a CpG binding protein with a ten-eleven translocase (TET) protein to identify methylated cytosine nucleotides.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

The invention provides a high-temperature-resistant uracil nucleotide kinase mutant and application thereof, a coding gene of the mutant, and application of the mutant in preparing uridine triphosphate from uracil nucleotide through enzyme catalysis. The method for preparing uridine triphosphate from the high-temperature-resistant UMP kinase mutant has the advantages of high yield, simple process, short period, convenience in industrial production and the like. The UMP kinase and the mutant thereof can be efficiently expressed in escherichia coli, the optimum enzyme activity temperature of the mutant enzyme is 70 DEG C, and the mutant enzyme shows relatively high tolerance to a high-temperature environment of 50-70 DEG C. Under the conditions that the reaction temperature is 50 DEG C, the substrate concentration is 150 mM and acetic acid kinase is added as a coenzyme, the conversion rate of uridine nucleotide catalyzed by enzyme liquid obtained by carrying out heat treatment on the UMP kinase mutant at 70 DEG C reaches 96% within only 2 hours, so that a good technical support is provided for industrial large-scale production of uridine triphosphate.