399 results about "Bisulfite" patented technology

There is disclosed a cancer diagnostic method based upon DNA methylation differences at specific CpG sites. Specifically, the inventive method provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.

A method for preparing a nucleic acid probe is provided. The method comprises forming an activated cytosine or cytidine by a bisulfite catalyzed reaction; and covalently linking a reporter molecule to the activated cytosine or cytidine, wherein said activating step, said covalently linking step, or both are conducted in the presence of microwave energy. Also provided by the invention are nucleic acid probes.

The invention discloses a construction method and an application of a high-throughout sequencing library. The construction method of the high-throughout sequencing library comprises the following steps: fragmenting genome DNA (deoxyribonucleic acid); repairing terminals of DNA fragments; adding a base A to 3' terminals of the DNA fragments with the repaired terminals; connecting the DNA fragments with sticky ends A to methylation linkers; carrying out hybrid capture on connecting products by using a specificity probe so as to obtain target fragments; carrying out bisulfite treatment on the target fragments so as to convert non-methylated cytosine in the target fragments to uracil; carrying out PCR (polymerase chain reaction) amplification on the converted target fragments; separating and purifying amplification products which form the high-throughout sequencing library. According to the method and application of the high-throughout sequencing library, the high-throughout sequencing library of a genome specific zone of a sample can be conveniently and efficiently constructed.

The invention provides a construction method and application of a genome-wide methylation high-throughput sequencing library. The construction method of the genome-wide methylation high-throughput sequencing library comprises steps of: conducting digestion on a genome DNA with Msp I and a second restriction endonuclease; conducting end repair on DNA fragments; adding a basic group A on a 3'terminal of the DNA fragment subjected to end repair; connecting a DNA fragment with a cohesive end A to a methylation joint; conducting fragment selection on the connect products with the methylation joint, in order to obtain a target fragment; subjecting the target fragment to a bisulfite treatment, in order to convert unmethylated cytosine in the target fragment to uracil; subjecting the converted target fragment to PCR amplification; and separating and purifying the amplification products, wherein the amplification products form the genome-wide methylation high-throughput sequencing library. The construction method and application of the genome-wide methylation high-throughput sequencing library provided by the invention can conveniently and effectively construct the genome-wide methylation high-throughput sequencing library of the genome DNA sample.

A catalytic cracking fume desulfuration wastewater treatment process is characterized in that wastewater is discharged to a slurry pool and is mixed with a flocculating agent, a stirrer is used for stirring the wastewater and the flocculating agent, the wastewater and the flocculating agent are mixed evenly, the wastewater after being mixed is discharged to an expansion tube type filter and is subjected to the coagulation reaction at the lower end of the expansion tube type filter, small particulate matters in the wastewater are flocculated, large flocculating bodies are formed, the solid-liquid separation is achieved through the filtration of a filtering film, thick slag and supernatant fluid are obtained, the supernatant fluid is discharged to an oxidation tank further, the thick slag is subjected to back washing and enters a subsequent filtering box, the thick slag is concentrated and dewatered in the filtering box, mud cakes which are concentrated are transported outwards, the water of dehydration returns to the slurry pool again, is mixed with the wastewater and is processed again, the false chemical oxygen demand (COD) which contains sulfite and bisulfite is oxidized and removed by the supernatant fluid in the oxidation tank, and the sewage which meets standards is discharged. Compared with the prior art, the process has the advantages that the area occupied by devices is small, the operation cost is low, the solid and the liquid can be separated completely, the working environment of workers is good, device parameters can be set in accordance with parameters of different materials, and the application is wide.

The present invention relates to a tag-modified bisulfite genomic sequencing (tBGS) method developed for simplified evaluation of DNA methylation sites. The method employs direct cycle sequencing of PCR products at kilobase scale, without conventional DNA fragment cloning. The method entails subjecting bisulfite-modified genomic DNA to a second-round PCR amplification employing GC-tagged primers. The invention also relates to a method for identifying a patient at risk for lung cancer using the tBGS technique disclosed.

A contaminant-removal filter for removing carbonyl-containing compounds from a gas stream, such as air. Examples of common airborne carbonyl-containing compounds include ketones, including acetone, and aldehydes, including formaldehyde. The filter has a porous or fibrous body that includes a plurality of passages extending from a first, inlet face to a second, outlet face, the passages providing flow paths. The body has a reactant material impregnated throughout the substrate. The reactant material is a sulfite, bisulfite, oxidant, or derivative of ammonia, specifically high molecular weight and stable amines. Strong alkali (basic) materials are particularly suitable for aldehyde removal. The filter is free of any humectants.

The invention relates to a method for removing organic pollutants in water at an ultrahigh speed. The method comprises the following steps: firstly, adding a reducing agent to water to be treated; secondly, adjusting the pH value of the water to be treated to be within the range of 3-7; thirdly, adding an oxidizing agent to the water to be treated. According to the method, bisulfite radicals are utilized for reacting with potassium permanganate or manganese dioxide, the removal of the organic pollutants in the water at the ultrahigh speed is realized by quickly generating a highly active oxidizing agent Mn(III) (at a non-complexing state), the rate of removing the pollutants by potassium permanganate and manganese dioxide can be improved by one thousand to one million times and is ten thousand times higher than the apparent oxidation rate of a general advanced oxidation technology, and the degradation for the pollutants can be completed in ten seconds or several seconds. According to the method, the pollutants incapable of being oxidized by potassium permanganate and manganese dioxide can also be oxidized, and even nitrobenzene capable of being oxidized by hydroxyl radicals only in traditional meanings can be oxidized, so that the oxidizing capacity of potassium permanganate and manganese dioxide is greatly improved, and the application range of potassium permanganate and manganese dioxide is expanded.

The present invention provides a method and assay for determining global methylation using the methylation status of repetitive DNA elements as a surrogate marker. Also provided by the invention is a method for determining efficacy of a DNA methylation inhibiting drug by using changes in repetitive DNA methylation as a marker for drug efficacy. Additionally, the invention provides nucleic acid compositions for performing the method.

The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.

Methods for determining the methylation status of a plurality of cytosines are disclosed. In some aspects genomic DNA target sequences containing CpGs are targeted for analysis by multiplex amplification using target specific probes that can be specifically degraded prior to amplification. The targets may be modified with bisulfite prior to amplification. In another aspect targets are cut with methylation sensitive or insensitive restriction enzymes and marked with a tag using the target specific probes. The presence or absence of methylation may be determined using methylation sensitive restriction enzyme or bisulfite treatment. Detection in many embodiments employs hybridization to tag arrays, genotyping arrays or resequencing arrays.

The invention relates to preparation and application methods of ratio-dependent bisulfite ion fluorescent probes, belonging to the technical field of anion detection. The probe molecules have recognition specificity for bisulfite ions in a neutral aquatic environment, generate fluorescence ratio variations, have very high sensitivity, and meanwhile, cause obvious color and fluorescent color variations. The fluorescent probes do not generate obvious variations for other ions, free radicals or molecules. The invention implements recognition specificity of the probe molecules for the bisulfite ions, provides the fluorescent probes with color comparison sensing function, and thus, has wide application prospects in the fields of biology and ion detection.

Antiviral protease inhibitors, including peptidyl aldehydes, peptidyl α-ketoamides, peptidyl bisulfite salts, and peptidyl heterocycles, are disclosed, along with related antiviral compounds, and methods of using the same to treat or prevent viral infection and disease. The compounds possess broad-spectrum activity against viruses that belong to the picornavirus-like supercluster, which include important human and animal pathogens including noroviruses, enteroviruses, poliovirus, foot-and-mouth disease virus, hepatitis A virus, human rhinovirus (cause of common cold), human coronavirus (another cause of common cold), transmissible gastroenteritis virus, murine hepatitis virus, feline infectious peritonitis virus, and severe acute respiratory syndrome coronavirus.

The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.

The present invention discloses one kind of lichi and longan preservative with high preserving effect, no toxicity, no environmental pollution and low cost and suitable for industrial production. The preservative consists of queensland arrowroot powder and bisulfite, pyrosulfite or dithionite capable of releasing SO2 when reacting with water and air. When the preservative is used, it is packed into non-woven polypropylene pocket, the pocket is set inside non-woven polypropylene bag for holding lichi or longan, and the bag is sealed and set into some packing box, with the amount of the preservative being 3.1-8.6 % of the weight of lichi or longan.

This invention provides methods and arrays for determination of the methylation patterns at single-nucleotide resolution by array-based hybrid selection and next-generation sequencing of bisulfite-treated DNA.

Described herein are methods, compositions and kits for the generation of bisulfite-converted libraries useful for conducting reduced representation bisulfite sequencing (RRBS). The methods described herein can be employed to generate RRBS libraries in a manner that is easier and more cost-efficient than conventional RRBS methods, and can be efficiently sequenced with next generation sequencing (NGS) techniques without the need for genomic, higher diversity sequencing controls such as PhiX spike-ins.

The invention provides a method for desulfurization of SO2 waste gas. A basic absorption liquid neutralizes the SO2- laden waste gas to eliminate SO2, discharge the purified gas when the content of SO2 reaches the standard; microbe reduces the desulfurizing absorption tail liquid (containing sulfite group, bisulphate group, or sulfuric group), then microbe oxygenizes the liquid, and the SO2 contained is recovered as a existent of elemental sulfur. The liquid treated by microbe is basic, and it can return directly to the desulfurizing absorption unit and be reused as a basic absorption liquid. And it realizes the aim of eliminating waste and recycling sulfur resource. The method for waste gas treatment is easy to operate and leads to none secondary pollution.

A stabilised composition comprising at least one adrenergic compound and at least one antioxidant selected from the group consisting of a bisulfite, a metabisulfite and a sulfite compound.

The present invention provides methods for improving the efficiency of methylation detection. The present invention provides improvements from current technologies via increased efficiency of template denaturation and bisulfite conversion reaction, also significant time savings in sample preparation, recovery, as well as increased efficiency of desulfonation. These methods facilitate rapid analysis of research and clinical samples and enhance the ability to process high-through put sample preparations. The methods are applicable to essentially all methylation detection procedures and also to the analysis of methylation patterns from various species.

The invention discloses a construction method, a kit and application of a plasma free DNA methylation detection library. The construction method comprises the following steps: S1 of extracting plasmafree DNA from a blood sample; S2 of performing tail end repair and 3' terminal A base addition on the plasma free DNA; S3 of connecting the tail ends of the plasma free DNA obtained in the S2 with C methylation modified connectors; S4 of performing bisulfite conversion on a product obtained in S3 and performing PCR expansion; S5 of performing magnetic bead purification on the PCR product of S4 andremoving small-fragment DNA which is not amplified in a non-specific mode and primer dimer; S6 of performing PCR amplification on a product of S5 and performing magnetic bead purification to obtain aplasma free DNA methylation detection library. By means of the technical scheme of the construction method disclosed by the invention, an initial amount of general microscale methylation library construction is reduced, library construction steps are simplified, library recycling efficiency is improved, and a foundation is established for sequentially screening tumor diagnosis markers through plasma free DNA methylation difference locus.

The invention provides a method for desulfurization from a SO2- laden waste gas and for recovery of the sulfur resource. A basic absorption liquid neutralizes the SO2- laden waste gas, discharge the purified gas when the content of SO2 reaches the standard; microbe reduces the desulfurizing absorption tail liquid (containing bisulphate group), and then the liquid is oxygenized with chemical treatment to recover elemental sulfur. The desulfurizing absorption tail liquid treated by microbe is basic, and it can return directly to the desulfurizing absorption unit and be reused as a basic absorption liquid; the chemical oxidant is oxygenized by microbe to revive. The invention realizes the aim of eliminating waste and recycling sulfur resource. And it is easy to operate and leads to none secondary pollution.

A substantially cyanide-free plating solution for depositing copper from the monovalent ionic state, which includes a source of copper ions, a reducing agent capable of reducing divalent copper ions to monovalent copper ions, an alkali material in an amount sufficient to maintain the pH of the solution in the range of about 7 to about 10, and a complexing agent of an imide, such as succinimide, 3-methyl-3-ethyl succinimide, 3-methyl succinimide, 3-ethyl succinimide, 3,3,4,4-tetramethyl succinimide, or 3,3,4-trimethyl succinimide, or a hydantoin, such as dimethyl hydantoin. The substantially cyanide-free plating solutions may also include at least one of a conductivity salt, an additive to promote brightness, or an alloying metal. The reducing agent may be an alkali sulfite, alkali bisulfite, hydroxylamine, or hydrazine. The copper is typically provided in the form of CuC1, CuC12, CuSO4, or Cu20 in an amount sufficient to provide a copper ion concentration of from about 2 to about 30 grams per liter of solution, and the complexing agent is present in an amount sufficient to provide a molar ratio of copper ions to complexing agent of from about 1:1 to about 1:5, preferably about 1:4. The alkali material is typically NaOH, KOH, NH4OH, or Na2CO3, and the conductivity salt is typically NaC1, KC1, Na2SO4, K4P2O7, Na3PO4, C6H5Na3O7, C6H11NaO7, NH4C1, or KNaC4H4O6. Useful additives include organic amines or oxyalkyl polyamines, such as triethylene tetramine, tetraethylene pentamine, and polyoxypropyl-triamine. Methods for preparing such a solution for plating copper onto a substrate, and of plating copper onto a substrate with such a solution are also disclosed.

The process of invention provides a simplified conversion of sulfur dioxide gas into hydrogen sulfide gas. First, sulfur dioxide gas is absorbed into an aqueous sulfide solution to form sulfite and bisulfide ions. Second, additional sulfur dioxide gas is absorbed into the aqueous solution to form hydrogen sulfide. Third, another portion of the sulfur dioxide is absorbed and reacts in the aqueous solution to form bisulfite. Most advantageously, the bisulfite is decomposed into sulfur dioxide and sulfite. The sulfite is then reduced to sulfide and returned for use in the absorption process. The hydrogen sulfide may then be reacted with SO2 via the Claus reaction to form elemental sulfur.