251 results about "Small fragment" patented technology

The invention relates to methods for isolating and amplifying small fragments of genomic DNA for genotyping polymorphisms in human populations.

A flash system has multiple channels of flash memory chips that can be accessed in parallel. Host data is assigned to one of the channels by a multi-channel controller processor and accumulated in a multi-channel page buffer. When a page boundary in the page buffer is reached, the page buffer is written to a target physical block if full, or combined with old data fragments in an Aggregating Flash Block (AFB) when the logical-sector addresses (LSA's) match. Thus small fragments are aggregated using the AFB, reducing erases and wear of flash blocks. The page buffer is copied to the AFB when a STOP command occurs. Each channel has one or more AFB's, which are tracked by an AFB tracking table.

Apparatus and methods of using lasers are provided for the perforation of oil and gas well casings and rock formations. A rock removal process called laser spallation is provided that utilizes a combination of laser-induced thermal stress and laser induced superheated steam explosions just below the surface of the laser/rock interaction to spall or fracture the rock into small fragments that can then be easily removed from the rock formation. The use of high power laser beams of kilowatt level is provided to rapidly cut the steel casings and perforate into the formation. Techniques of the invention increase permeability and reduce hole tapering while perforating a deep hole in reservoir rock formations.

Apparatus and methods of using lasers are provided for spalling and drilling holes into rocks. A rock removal process is provided that utilizes a combination of laser-induced thermal stress and laser induced superheated steam explosions just below the surface of the laser/rock interaction to spall the rock into small fragments that can then be easily removed by a purging flow. Single laser beams of given irradiance spall rock and create holes having diameter and depth approximately equal to the beam spot size. A group of the single laser beams are steered in a controllable manner by an electro-optic laser beam switch to locations on the surface of the rock, creating multiple overlapping spalled holes thereby removing a layer of rock of a desired diameter. Drilling of a deep hole is achieved by spalling consecutive layers with an intermittent feed motion of the laser head perpendicular to the rock surface.

The invention discloses a construction method of a ctDNA ultra-low-frequency mutation detection library, a kit and an analysis method of library detection data. The construction method comprises steps as follows: S1, cfDNA is extracted from whole blood; S2, the terminal of cfDNA is restored and an A basic group is added to the 3'terminal; S3, the terminal of the cfDNA obtained in S2 is connected with connectors containing random label sequences; S4, primers for multiplex PCR (polymerase chain reaction) are designed according to the sequences of the connectors and an object region for object region capturing; S5, a PCR product in S4 is subjected to magnetic bead purification, and small-fragment DNA not subjected to non-specific amplification and primer dimer are removed; S6, a product in S5 is subjected to PCR amplification, an index sequence is introduced, and the ctDNA ultra-low-frequency mutation library is obtained. According to the method, PCR mistakes and sequencing mistakes are eliminated, and the detection specificity is improved.

A clean dry glass powder useful as a substitute for Portland cement in concrete, in paints, and for other known uses for glass powder produced conventionally can be produced from unsorted post-consumer waste glass, including a subsatantial fraction of non-glass items, by employing glass pulverizing equipment to reduce waste glass to small fragments, allowing removal of trash, employing a multistep washing process to clean the glass fragments, in the preferred embodiment using aggregate cleaning equipment, drying the fragments, preferably using fluidized bed techniques, and grinding the glass to a desired particle size, preferably using a ball mill, in combination with an air classification step to produce a glass powder of uniform particle size.

A full-insurance rain bomb fuze relates to an artificial hail-suppressing rain bomb fuze for antiaircraft guns. The present invention mainly includes a fuze body, an upper drug tray, a lower drug tray, a rotary body, a bracket, a pressure screw, and a detonator tube. There is a 134-136° circular groove on the bottom surface of the medicine tray and the top surface of the medicine tray respectively. The gunpowder column is installed on the side, and the gyratory body is installed in the medicine feeding tray. The left and right sides of the medicine feeding tray and the gyratory body are respectively equipped with centrifugal safety screw plugs, centrifugal springs, centrifugal pins and safety pins, and gunpowder safety screw plugs. The dimensions of the booster tube have also been improved. The invention has the advantages of small fragments, sufficient explosion, safe use, good delay reliability and more complete detonation.

Method for processing and packaging garlic, comprising selecting a garlic bulb comprising at least one clove, wherein the clove is contained within a membranous tunic, and has a stem end and a root end; removing the tunic from the glove thereon, to obtain a peeled garlic clove; cutting off a small fragment of the stem end of the clove; washing the peeled clove; surface-sterilizing the peeled clove; packaging the peeled clove in a plastic bag; and sealing the bag under vacuum. Also disclosed are an improved method for storing and transporting the processed garlic cloves, as well as the processed garlic cloves.

The invention discloses a low-frequency gene fusion detection method and a low-frequency gene fusion detection device. The detection method comprises the following steps: S1, extracting cfDNA from whole blood; S2, performing tail end repairing on the cfDNA and adding A basic group at the 3' end; S3, connecting a joint containing a random label sequence; S4, designing a multiplex-PCR primer and performing target area capture; S5, performing magnetic bead purification on the PCR product in the S4, removing small fragment DNA not subjected to non-specific amplification and a primer dimer, and introducing an index sequence to obtain a ctDNA ultralow-frequency mutation library; S7, performing computer sequencing; S8, performing clustering analysis on data after library sequencing through the random label sequence; and S9, entering variation detection analysis after the quality control of the remaining data is qualified. By application of the technical scheme of the invention, the low-frequency mutation detection sensitivity is improved.

A fragmentation warhead comprising manual selection means for generating larger fragments versus smaller fragments upon detonation. The warhead includes a generally cylindrically shaped fragmenting metal outer warhead within which lies a generally cylindrically shaped explosive charge. Cylindrically arranged ring mechanisms within the warhead may be rotated to select desired fragmentation patterns.

Methods and systems for non-intrusively detecting the existence of fissile materials in a container via the measurement of energetic prompt neutrons are disclosed. The methods and systems use the unique nature of the prompt neutron energy spectrum from photo-fission arising from the emission of neutrons from almost fully accelerated fragments to unambiguously identify fissile material. The angular distribution of the prompt neutrons from photo-fission and the energy distribution correlated to neutron angle relative to the photon beam are used to distinguish odd-even from even-even nuclei undergoing photo-fission. The independence of the neutron yield curve (yield as a function of electron beam energy or photon energy) on neutron energy also is also used to distinguish photo-fission from other processes such as (γ, n). Different beam geometries are used to detect localized samples of fissile material and also fissile materials dispersed as small fragments or thin sheets over broad regions. These signals from photo-fission are unique and allow the detection of any material in the actinide region of the nuclear periodic table.

The invention discloses a rapid assembling method of multi-fragment DNA yeast. The rapid assembling method comprises the following steps: (1) performing co-transformation on a plurality of DNA molecules with homologous arms, and linearized yeast shuttle vectors, so as to obtain yeast; (2) eluting all the transformed yeast colonies on a whole screening culture plate, centrifuging, and discarding the supernate to obtain transformed yeast cells; (3) extracting plasmid DNA of the yeast cells obtained in step (2), transforming the competent cells of colon bacillus; and (4) screening colon bacillus, and cloning to obtain large-fragment DNA. The method for assembling large-fragment DNA molecules is fast in speed, simple, convenient and feasible, high in success rate, low in cost, high in efficiency, easy to operate, beneficial in enlarging the industrialization scale and wide in application, and a plurality of small-fragment DNA molecules can be assembled into one large-fragment DNA molecule.

The invention discloses a corn quick cooking rice gruel and a production method thereof, aiming at solving the problem of single nutrition of the existing fast food. The production method is characterized in that: a. rice, sorghum rice and unpolished rice do not need processing; b. cowpea, mung beans, small red beans, black kerneled rice, small fragments, myotonin and sticky rice are cleaned and edulcorated; the methods of screening, air separation and the like are adopted to remove impurities and imperfect grains and grains damaged by disease and pests; the provisions are dipped in water to moisten the provisions, dried by a microwave and naturally cooled by radiation and burned grains are removed; e. the provisions are mixed evenly by the following weight proportion: 60% of the rice, 10% of the cowpea, 10% of mung beans or the small red beans, 10% of the black kerneled rice and 10% of one of the sorghum rice, the small fragments, the myotonin, the unpolished rice and the sticky rice; and d. the mixture is vacuumized. The corn quick cooking rice gruel is rich in nutrition, convenient in eating and can be produced continuously.

The invention discloses a process for preparing ethanol by solid fermentation and distillation of sweet sorghum stalks, which comprises, (1) comminuting the harvested mature sweet Chinese sorghum stalk into small fragments with comminuting machines, (2) charging yeast activating liquid, (3) loading the fermentation raw material into fermentation tank and sealing, (4) distilling the fermented raw material in distillation flask.

The invention discloses a construction method, a kit and application of a plasma free DNA methylation detection library. The construction method comprises the following steps: S1 of extracting plasmafree DNA from a blood sample; S2 of performing tail end repair and 3' terminal A base addition on the plasma free DNA; S3 of connecting the tail ends of the plasma free DNA obtained in the S2 with C methylation modified connectors; S4 of performing bisulfite conversion on a product obtained in S3 and performing PCR expansion; S5 of performing magnetic bead purification on the PCR product of S4 andremoving small-fragment DNA which is not amplified in a non-specific mode and primer dimer; S6 of performing PCR amplification on a product of S5 and performing magnetic bead purification to obtain aplasma free DNA methylation detection library. By means of the technical scheme of the construction method disclosed by the invention, an initial amount of general microscale methylation library construction is reduced, library construction steps are simplified, library recycling efficiency is improved, and a foundation is established for sequentially screening tumor diagnosis markers through plasma free DNA methylation difference locus.

The invention discloses a foreign body detection system for an airfield runway. The system consists of a radar transmission and receiving antenna, a radar transceiver front-end, a cantilever, a rotating mechanism, a signal processor and a display control terminal, wherein the radar transmission and receiving antenna is connected with the radar transceiver front-end through a waveguide; the radar transceiver front-end is connected with the signal processor through the rotating mechanism; and the signal processor is connected with the display control terminal. For a linear frequency modulation continuous wave system, a high range resolution is obtained by wideband linear frequency modulation, and a synthetic aperture is constructed by the rotation of the antenna along a semicircular trajectory, so that a high azimuth resolution is obtained, the area of a ground clutter unit is reduced, and a small fragment target on the runway can be detected.

A long-distance blast banger including a cartridge loaded with a blast banger made up from plastic material. The blast banger encompasses a sound explosives cup and a delay cup. The sound explosives cup is loaded with a sound explosives agent having components including perchloryl potassium, magnesium powder and aluminum powder. A sufficient sound value is acquired through appropriate compacting of the sound explosives cup, and therefrom realizes uniform shattering of the cup into small fragments that are insufficient to cause injury. Furthermore, at least two helical grooves extending from a bottom to a top of the cartridge are defined on an inner wall thereof, and ear attachments are configured on a base of an outer wall of the blast banger so as to correspond with the helical grooves, thereby engendering a spinning stability effect during flight time of the blast banger, reducing wind resistance, and thus increasing flight range.

International regulations for aerofoils within gas turbine engines require the safe containment of a released aerofoil. The blade fragments must be contained within an engine casing. Smaller fragments will generally be easier to contain within the casing and therefore reduce the weight of that casing. However introducing lines of weakness may result in cavities and holes which are subject to moisture ingress and problems associated therewith. By providing a root section which incorporates a core having shear surfaces, blades can be designed which in normal use are subject to compressive loads and remain operational, but when subject to impact loads or bending forces create tension forces which cause fragmentation along the shear surfaces after initial energy losses by slippage. By providing the shear surfaces in cores their location is encapsulated avoiding problems with moisture ingress.