4 results about "Small fragment" patented technology

The invention discloses a data security transmission method based on an encryption algorithm, and relates to the technical field of data encryption transmission, and the method comprises the steps: carrying out the comparative analysis of a reduced fragment number of each small fragment and an original transmission number, marking an abnormal fragment, generating a transmission number comparison table according to a calibration number and the original fragment number, and carrying out the transmission of the abnormal fragment. In the data transmission process, each fragment is endowed with a unique identifier, encrypted transmission is performed on data, secure transmission of the data is ensured, and specific processing is performed on the transmission number of the fragment subjected to interception or interference operation, so that a receiving end can identify that the data is possibly interfered, and corresponding measures are taken for processing, thereby improving the data transmission efficiency. And after the data transmission is completed, the received serial numbers are subjected to reduction operation and are compared and analyzed with the transmission serial number comparison table, so that whether each fragment is transmitted normally or not can be accurately judged, and abnormal fragments can be found and processed in time.

The present invention discloses a method for enriching small fragment cDNA of single cell sequencing for identifying miRNA expression in single cell transcriptome, using SPRISlect magnetic beads to sort library fragments, adding PEG10000, isopropanol and trichlorohexammine cobalt complex to the magnetic bead binding solution to improve the enrichment of small fragments of 100 to 300 bp. The present invention innovatively integrates DNA linear amplification and magnetic bead small fragment sorting technology with 10xGenomics single cell library construction method, solving the problem of small fragment PrimRNA data loss caused by insufficient small fragment enrichment ability in the single cell sequencing process in the prior art. The present invention has broad application prospects.

The invention relates to the technical field of gene engineering, in particular to a plasmid point mutation or small fragment insertion method, which is characterized in that a specific primer is designed at a mutation or insertion site, another primer is designed in other non-target regions of a plasmid to form upstream and downstream pairing with the specific primer, and the distance between the other non-target regions and the insertion site is 500-1000bp; carrying out a first-step PCR reaction on the plasmid by using the specific primer and the other primer; a PCR reaction product is recovered, and a DNA double-chain fragment is obtained; and carrying out a second-step PCR reaction on the plasmid by taking a DNA double-chain fragment as a large primer, and recovering and digesting a PCR product, so as to convert. The method can realize 100% correct rate of plasmid point mutation or small fragment insertion, is an efficient method for plasmid point mutation or small fragment insertion, and has a good promotion effect on molecular biology.