2 results about "Small fragment" patented technology

The invention relates to the technical field of gene engineering, in particular to a plasmid point mutation or small fragment insertion method, which is characterized in that a specific primer is designed at a mutation or insertion site, another primer is designed in other non-target regions of a plasmid to form upstream and downstream pairing with the specific primer, and the distance between the other non-target regions and the insertion site is 500-1000bp; carrying out a first-step PCR reaction on the plasmid by using the specific primer and the other primer; a PCR reaction product is recovered, and a DNA double-chain fragment is obtained; and carrying out a second-step PCR reaction on the plasmid by taking a DNA double-chain fragment as a large primer, and recovering and digesting a PCR product, so as to convert. The method can realize 100% correct rate of plasmid point mutation or small fragment insertion, is an efficient method for plasmid point mutation or small fragment insertion, and has a good promotion effect on molecular biology.