The invention discloses a precise construction strategy of a high-sensitivity
DNA circulation inducible fluorescent nanorobot, which specifically comprises the following steps: A1, constructing
a DNA nanostructure framework which is composed of a modularly designed recognition domain, a
signal conversion domain and a circulation trigger domain, the recognition domain comprises an
aptamer or a hairpin structure and is used for specifically combining with a target molecule, and the circulation trigger domain is used for specifically combining with the target molecule; the invention relates to the technical field of
gene engineering. According to the precise construction strategy for the high-sensitivity
DNA circulating inducible fluorescent nano-
robot, through integration of a modular
DNA tetrahedral framework and an
enzyme digestion-strand displacement synergistic amplification
system, cyclic utilization and
signal exponential
gain (such as CHA
cascade amplification combined with
CRISPR-Cas12a trans-cleavage) of a target object are achieved, the
detection limit breaks through the 0.1 am level, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-
robot is greatly improved, so that the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-
robot is improved, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is improved. Compared with a traditional single
enzyme digestion or
linear amplification method, the sensitivity is improved by at least three orders of magnitude. Meanwhile, time-resolved
upconversion nanoparticles (UCNPs) are combined with a
magnetic bead separation-lock type probe
noise reduction technology.