364 results about "Linear amplification" patented technology

The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification. The method of the invention includes the steps of forming at least a first cleavage structure comprising at least one flap, cleaving the cleavage structure with a cleavage means to release the at least one flap and detecting the at least one flap. In one embodiment of the invention, the method includes the additional steps of forming at least a second cleavage structure comprising the at least one released flap from the first cleavage structure cleaving the second cleavage structure with a cleavage means to release at least one flap from the second cleavage structure and detecting released flaps from the first or second cleavage structure.

Described herein are novel polynucleotides associated with prostate cancer. The polynucleotides are miRNAs, miRNA precursors, and associated nucleic acids. Methods and compositions are described that can be used for diagnosis, prognosis, and treatment of prostate cancer. Also described herein are methods that can be used to identify modulators of the disease-associated polynucleotides. Also described herein are methods and compositions for linear amplification and labeling of a targeted nucleic acid. The amplified targeted molecules may be used in hybridization techniques like Luminex and Microarray analysis.

Compositions and methods for amplifying selected polynucleotides, including DNA and RNA, particularly in multiplex amplification reactions using common primers amplification. Generally, methods of the invention employ multiple steps such as template-specific hybridization, a linear amplification, partial degradation of nucleic acid, and ligation. At the end of the process the sequences of selected polynucleotides are flanked by the common sequences which can be used for exponential amplification using common primers. In some aspects the polynucleotides are associated with a barcode and the presence of the barcode is detected to measure the amount of the polynucleotide.

The present invention relates to an in-vessel or down-hole optical imaging sensor or system for operating in structures which may contain media with different spectral transmission characteristics. The imaging sensor of the present invention selectively emits and/or detects two or more independently controllable wavelengths or wavebands. The imaging sensor comprises an illuminator for emitting radiation of a specified wavelength or waveband through a medium to a target, at least one detector for detecting the radiation deflected by said target and at least one amplifier for providing non-linear amplification of the detector output.

The invention relates to a pulse electronic accelerator nano-pico ampere electron beam flow measuring system, relating to an electronic accelerator pulse electron beam flow measuring system, in particular to the nano-pico (10-10A/cm2) micro beam flow measurement of millisecond pulse, belonging to the electron radiation environment ground simulation and test field. The pulse electronic accelerator nano-pico ampere electron beam flow measuring system is composed of a novel inclined-bottom faraday cup, three coaxial cables and a nano-pico level current amplifier. The measurement result can be obtained by oscilloscope or data acquisition card. The inclined-bottom faraday cup electron collection system resolves the collection efficiency problem of high energy electrons (MeV), to improve collection efficiency. The adopted three coaxial cables can reduce the interference of electromagnetic field. The nano-pico ampere level current amplifier resolves the stable current linear amplification problem under electron radiation, and converts weak current signals to voltage signals, thus being convenient for data acquisition of oscilloscope or data acquisition card. The invention resolves the measurement problem of nano-pico ampere pulse electron beam flow, to confirm the accuracy, real-time property and easy use of measurement.

An RF power amplifier has a final-stage amplifier stage which generates an RF transmit output signal, a signal detector which detects an RF transmit output level, a first detector, a second detector and a control circuit. The final-stage amplifier stage includes a transistor and a load element and performs saturation type nonlinear amplification and non-saturation type linear amplification. The first detector and the control circuit maintain the RF transmit output signal approximately constant with respect to a variation in load at an antenna at the saturation type nonlinear amplification. The second detector and the control circuit reduce an increase in the output voltage of the final stage transistor with respect to an overload state of the antenna at the non-saturation type linear amplification.

A motor control apparatus of the present invention comprises a velocity detector for detecting a rotation velocity of a mover of a motor, a position detector for detecting a position of the mover, a differentiator for performing time differentiation with respect to the position detected by the position detector to obtain a velocity calculation value, a feedback signal generating circuit for generating a feedback signal including the velocity detection value obtained by the velocity detector as a high frequency component and the velocity calculation value obtained by the differentiator as a low frequency component, and a linear amplifier and an integrating amplifier for performing PI control, the linear amplifier and the integrating amplifier performing linear amplification and integrating amplification of a difference between the velocity instruction and the feedback signal, respectively.

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

The invention relates to a method and an instrument for measuring the shock ground resistance of a transmission line tower. The method comprises the following steps: generating incident current by a shock current generator and filling the incident current into the ground; collecting the grounding voltage and the current of the tower by a data collecting device; carrying out treatment and linear amplification on the collected data by an MCU control panel to solve the voltage response of a grounding body under the action of standard lightning current and obtain an equivalent voltage um and a current peak im; and finally, obtaining the shock ground resistance of the tower according to a formula of Rch=um/im. The instrument comprises a current pole lead connecting plug, a grounding device connecting plug, controlled silicon, a charging board, a voltage-multiplying generator, a voltage collecting board, a current collecting board, a battery and a main control board, wherein the current polelead connecting plug is connected with the charging board and the voltage-multiplying generator and then connected to the main control board; the controlled silicon is connected to a circuit in series; the voltage collecting board is connected with a voltage pole lead connecting plug, the grounding device connecting plug and the main control board; the current collecting board is connected with the current pole lead connecting plug, the grounding device connecting plug and the main control device; and the battery is directly connected to the main control board.

Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification of the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

The invention provides a combined pre-coding and power allocation method of the multi-cast network based on the network coding in the wireless communication field. In the invention, a multi-cast network formed by two-source, two-place and arbitrary relaying cluster is adopted. Two sinks need to obtain the information of each source and can not cross-transmit the signal due to the limit of the sending power, so the relaying node is responsible for forwarding information of each source. Through introducing simulation network coding into the relaying, namely, the received aliasing signal of the two sources is broadcast out after linear amplification, thus minimizing the transmission time to the maximum extent. The invention improves the network error frame performance and enhances the overall through put of the network by introducing the combined pre-coding and power allocation.

A spaceflight optical remote sensing imaging simulation method based on a space-time unified feature comprises the steps that (1), the relation of the position of a satellite at any GMT and the time and the longitude and latitude of the satellite at a GMT sub-satellite point are obtained in a calculating mode according to satellite orbit parameters; (2), the entrance pupil radiance L (lamda) of a remote sensor is obtained in a calculating mode according to the longitude and latitude of the satellite at the GMT sub-satellite point, an imaging time ground target reflectivity feature pho and a solar elevation angle theta; (3), the signal Starget of the remote sensor is obtained by combining the remote senor parameters and L (lamda), linear amplification, smoothing and quantization processing are carried out on the Starget in sequence, and an initial simulation image is obtained after a remote sensor optical system, a remote sensor detector, a remote sensor circuit and a simulation MTF of a satellite platform are overlaid; (4), after compression, uncompression, radiation correction and MTF compensation are carried out on the initial simulation image, a simulation image needed by a user is obtained. The method can carry out effective optical remote sensing full-chain imaging simulation, and optical remote sensing in-orbit imaging quality is greatly improved.