47 results about "Linear amplification" patented technology

The invention relates to a differential voltage time-digital converter for preventing large based on Double-tailed, and the differential voltage time-digital converter comprises a Double-tailed dynamic latch comparator which carries out the linear amplification of an input differential voltage, and outputs a differential voltage; a voltage time domain converter (VTC) which converts the output differential voltage into a time difference signal and outputs a first delay voltage and a second delay voltage; the input end of the polarity judging device is connected with the VTC, and the polarity judging device is used for outputting a polar code as the highest bit of a thermometer code; the input end of the edge selector ELS is connected with the VTC, and the edge selector ELS is used for generating an EARLY signal and a LATE signal according to the first delay voltage and the second delay voltage; and the input end of the delay chain type time-to-digital domain converter TDC is connected with the edge selector ELS, and the delay chain type time-to-digital domain converter TDC is used for quantizing the time difference between the EARLY signal and the LATE signal and generating a thermometer code. According to the invention, the idea of high-speed latch is introduced to obtain stable differential input voltage with smooth waveform, so that the VTC module can carry out time domain transformation on the differential input voltage, and the resolution of the TDC is improved.

The invention relates to the technical field of road safety facilities, in particular to a gradient effect concave deceleration strip visual grading mark design method which comprises the steps that S1, basic design data of a target road scene are obtained, and the basic design data comprise a target limited vehicle speed numerical value and a total length numerical value of a deceleration area; s2, according to the basic design data, geometric configuration parameters of a plurality of concave speed reduction units sequentially arranged in the vehicle advancing direction are calculated, and the geometric configuration parameters comprise the concave depth numerical value of each concave speed reduction unit and the distance numerical value between every two adjacent concave speed reduction units; the concave depth value obtained through calculation presents a gradually increasing trend in the vehicle advancing direction. According to the method, the concave speed reduction units with the gradually-increased depth and the gradually-decreased interval are configured through an algorithm, the overspeed vehicle is forced to decelerate through non-linear amplified composite vibration, and the control efficiency of road section speed limiting is improved.

The application is suitable for the biotechnology field, and provides a multiplex amplification method and system and application, wherein the multiplex amplification method comprises the following steps: linear amplification of a target template by using an upstream primer mixture designed for multiple target regions to obtain a first product by purification; the 5' end of the upstream primer contains a universal primer sequence, and the 3' end contains a specific target recognition sequence; linear amplification of the first product by using a downstream primer mixture designed for multiple target regions to obtain a second product by purification; the 5' end of the downstream primer contains a universal primer sequence, and the 3' end contains a specific target recognition sequence; and unified exponential amplification of the second product by using a universal primer. The application innovatively adopts a specific multi-target molecular marker combined with a universal primer amplification strategy to establish a brand-new multi-target DNA fragment amplification technology system, and high uniformity, high specificity and high-throughput multi-target amplification can be realized.

The invention discloses a vibration wire sensor health state diagnosis device and method, and relates to the technical field of sensor state diagnosis, the device comprises a main processor, a vibration signal generation module, a linear amplification circuit and a saturation amplification circuit, the vibration signal generation module generates an excitation modulation signal so as to enable a vibration wire of a vibration wire sensor to vibrate, and the linear amplification circuit is connected with the saturation amplification circuit; a vibrating wire vibration signal of the vibrating wire sensor is collected through the signal processing unit, a linear amplification signal and a multiple harmonic signal are obtained through the linear amplification circuit and the saturation amplification circuit, and the main processor carries out time domain and frequency domain signal processing on the linear amplification signal and the multiple harmonic signal. And obtaining a signal-to-noise ratio value and the duration time of each harmonic signal, and judging and diagnosing the health state of the vibrating wire sensor according to the time domain and frequency domain signals in the linear amplification signal and the multiple harmonic signals, the signal-to-noise ratio value and the duration time of each harmonic signal, so that automatic diagnosis of the health state of the vibrating wire sensor is realized.

The invention is applicable to the technical field of biology, and provides a multiple amplification method and system and application wherein the multiple amplification method comprises the following steps: performing linear amplification and purification on a target template by using an upstream primer mixture designed for a plurality of target regions to obtain a first product; the 5'end of the upstream primer contains a universal primer sequence, and the 3 'end of the upstream primer contains a specific target recognition sequence; performing linear amplification on the first product by using a downstream primer mixture designed for a plurality of target areas, and purifying to obtain a second product; the 5'end of the downstream primer contains a universal primer sequence, and the 3 'end of the downstream primer contains a specific target recognition sequence; and performing uniform exponential amplification on the second product by using a universal primer. According to the invention, a brand new multi-target DNA fragment amplification technology system is established by innovatively adopting a specific multi-target molecular marker and combining a universal primer amplification strategy, and high-uniformity, high-specificity and high-throughput multi-target amplification can be realized.

The invention discloses an ultrasonic flow measuring device based on a transfer function waveform optimizer, and belongs to the technical field of ultrasonic flow measurement. The problem that the flow measurement precision of an existing ultrasonic flow measurement device needs to be improved is solved. The ultrasonic flow measuring device comprises a transmitting transducer, a receiving transducer, a waveform optimizer and a linear amplification excitation module. The waveform optimizer optimizes an excitation waveform and outputs an excitation signal through the waveform generator, and is used for enabling the output signal of the receiving transducer to present a stable sine section and / or a high signal-to-noise ratio section; and the linear amplification excitation module performs linear amplification excitation on the output signal of the waveform generator, so that the transmitting transducer can reproduce the excitation signal output by the waveform generator.

The invention relates to an array type monitoring device for a loose part of a nuclear power station. The array type monitoring device comprises a channel selection array module and a channel switching array module which are integrated in a processor. The channel selection array screens target signals from multiple sound channels and playback channels through a preset logic (based on signal intensity and key areas), and supports single-channel extraction or multi-channel fusion; the channel switching array realizes smooth interference-free switching between real-time signals and between real-time and historical playback signals based on a special algorithm. The device adopts a 2U rack type design and is internally provided with a dual-power-amplifier system; a D-type power amplifier drives left and right sound channel loudspeakers by a high-frequency switch technology, and the power consumption is less than or equal to 75W; the class AB power amplifier drives an earphone interface through linear amplification, and the distortion rate is lt; 6%. And the integrated storage module supports historical sound data backtracking analysis. The problems that existing equipment is insufficient in multi-channel processing, inflexible in switching and poor in power amplifier adaptability are solved, and the comprehensiveness, timeliness and accuracy of nuclear power station loose part monitoring are comprehensively improved.

The invention relates to the technical field of discrete manufacturing industry production process control, and discloses an automatic collaborative regulation system for a discrete manufacturing industry production process, which comprises a node control unit distributed at a process node and a collaborative control resolving unit, the cooperative control calculation unit converts the node operation rhythm into a time axis distribution parameter in a sequential logic correlation model, calculates a target adjustment vector based on the parameter, and superposes a compensation pulse at the output leading edge of the target adjustment vector by using a feed-forward shaping mechanism, so that an adjustment signal breaks through a physical execution dead zone of a bottom-layer driving execution unit, and the control of the bottom-layer driving execution unit is realized. According to the method, through signal shaping and smoothing of the dynamic impedance difference between the ideal control law and the mechanical actuator, the non-lagging response to extremely fine disturbance is realized, the non-linear amplification of errors in a rigid time sequence frame is blocked, and the stable convergence of a control loop is ensured.

The invention discloses a temperature control circuit of an oven controlled crystal oscillator, which is characterized in that on the basis of a traditional thermosensitive bridge and a heating circuit, a switch driving mode of a driving circuit is changed into a linear mode, namely a linear voltage comparison circuit is added, and the linear voltage comparison circuit not only has a linear amplification function, but also has a PID (Proportion Integration Differentiation) calculation function. In addition, the heating circuit is connected with the voltage-controlled current source circuit. When the thermosensitive bridge is unbalanced, the thermosensitive bridge generates a voltage signal difference related to the temperature, the voltage difference drives a heating power tube of the heating circuit after being subjected to linear amplification and PID calculation through the linear voltage comparison circuit, and when the set temperature is reached, the thermosensitive bridge and the linear voltage comparison circuit output constant voltage. The heating circuit generates constant heating power, and the whole circuit reaches a balanced state. According to the invention, after the voltage difference is regulated and controlled through the linear comparison circuit, the heating power tube is controlled, and the control is not an on state or an off state, but is related to the amplitude of the voltage difference, so that the temperature control precision is improved, and under the cooperation of the PID circuit, the temperature overshoot phenomenon is well inhibited.

The invention belongs to the technical field of power supply circuits, and particularly relates to a four-quadrant direct-current linear adjustable power supply device and a working method thereof.The four-quadrant direct-current linear adjustable power supply device comprises a linear amplification circuit, a positive power supply selection circuit, a positive power supply adjustment circuit, a negative power supply selection circuit, a negative power supply adjustment circuit and an output stage circuit; an input signal is amplified to the output stage circuit through the linear amplification circuit, so that the positive power supply selection circuit outputs a corresponding adjustable positive voltage to a load through the positive power supply adjustment circuit and the output stage circuit, or the input signal is amplified to the output stage circuit through the linear amplification circuit; the negative power supply selection circuit outputs a corresponding adjustable negative voltage to a load through the negative power supply adjustment circuit and the output stage circuit; adjustable input positive voltage and input negative voltage can be obtained, meanwhile, the linear amplification circuit is matched with the output stage circuit to achieve linear continuous adjustable output within the range of + / -210V, and the wide-range, high-precision and four-quadrant working capacity is achieved.

The application provides a photochemical reactor, method and system with total reflection characteristics, and relates to the technical field of photochemistry.The application aligns a light source with the direction of a reaction fluid, and uses total reflection conditions to constrain light energy in a reaction system, thereby forming an orderly propagating optical waveguide structure, and fundamentally solving the problem of serious loss caused by disordered scattering of light energy, wall absorption and transmission in the prior art.Orderly and efficient use of light energy enables faster reaction rate under the same light intensity, or higher light intensity can be used under small pipe diameter, thereby greatly improving reaction efficiency and shortening reaction time.The reactor structure of the application is only a simple pipe structure, and does not need complex optical fiber coupling or nested channels, and linear amplification can be realized through simple series connection or parallel connection without obvious adverse amplification effect.Two total reflection modes can be flexibly selected to adapt to reaction systems with different refractive indexes, are widely applicable, and have simple structure and low energy consumption, and meet the requirements of green chemistry.

The application belongs to the technical field of quantum communication, and in particular relates to a polarization-frequency two-degree-of-freedom encoding coherent state noiseless linear amplification method. The parallel amplification idea is used. The original polarization-frequency two-degree-of-freedom encoded coherent state is split into N paths through a beam splitter array. The coherent state in each path becomes a weak coherent state. A PC amplifier and an auxiliary photon are arranged on each path. The photon state of each path is amplified. If all the PC amplifiers on all the paths operate successfully, the output states of all the amplifiers are converged on a path through another beam splitter array. Finally, a new coherent state is extracted. The application can effectively improve the average photon number in the output coherent state, realize amplification of the coherent state, and perfectly preserve the information of the photon encoding in the polarization and frequency two degrees of freedom. In addition, the application has strong practicability.

The embodiment of the invention discloses a wide-range pre-amplification circuit with a temperature compensation function and photoelectric detection equipment. The wide-range pre-amplification circuit comprises a cross-group amplification module, a filtering module and a logarithmic amplification module which are connected in sequence; for a weak light current signal in a large dynamic range, a transimpedance amplification module is designed by using an operational amplifier to carry out I / V conversion circuit, so that signal distortion caused by parasitic capacitance and gain resistance of a photoelectric conversion device is avoided; a logarithmic amplification module and a temperature compensation module designed by using a cut-off and amplification region of a triode are used for performing piecewise linear amplification on a voltage signal output by a transimpedance amplification module, high gain is used for amplifying when a small signal is input, and low gain is used for amplifying when a large signal is input so as to meet the input range of a high-precision ADC; the problem of high-precision measurement of wide-range weak signals is solved, and the temperature stability of the double-slope logarithmic amplification circuit is improved by utilizing the temperature compensation module.

The application provides an envelope tracking method and device, the method obtains a target envelope tracking input current signal through a boost circuit, and transmits the target envelope tracking input current signal to an amplification circuit through the boost circuit; the amplification circuit comprises an operational amplifier and a feedback network, and the operational amplifier works in a floating ground mode; the amplification circuit performs closed-loop conversion amplification on the target envelope tracking input current signal, and outputs an envelope tracking output voltage; the application also provides an envelope tracking device, and solves the problem that in the prior art, when the envelope tracking output voltage amplitude is high and the swing is large, the operational amplifier has high loss and low efficiency in the linear amplification process, so that the target envelope tracking input current signal is converted and amplified more efficiently, the conversion efficiency of the operational amplifier is improved, the amplification loss is reduced, and the user experience is improved.

The invention discloses a miRNA detection kit and method based on Cas12a self-circulation and CHA double cascade amplification, and belongs to the technical field of biosensing. The method comprises the following steps: firstly, converting target miRNA into a double-stranded DNA activator by utilizing self-assembly of a catalytic hairpin; furthermore, a CRISPR-Cas12a positive feedback self-circulation system based on specially designed scgRNA is triggered, so that exponential amplification of a signal is realized; finally, the Au-coated CuO core-shell nano-enzyme with high catalytic activity is released by cutting a connecting chain, and TMB-HO chromogenic reaction is catalyzed, so that colorimetric detection is realized. According to the present invention, the linear amplification mode and the index amplification mode are ingeniously fused, the heterojunction nano-enzyme is used to enhance the signal, and the kit has advantages of high sensitivity (the detection limit can achieve 1.39 fM), strong specificity, simple operation, no complex instrument, visual detection realization and the like, and has wide application prospects in the fields of early cancer screening and immediate cancer diagnosis.

The utility model provides a fault monitoring device for equipment voiceprint analysis, which comprises a shell, and a pickup unit, a linear amplification circuit, a signal processing unit, a control unit, a feedback unit and a power supply unit are arranged in the shell. The signal processing unit comprises a filter circuit, an analog-to-digital conversion circuit and an audio processing unit; a signal obtained by the pickup unit is transmitted to the signal processing unit after passing through the linear amplification circuit, the output of the signal processing unit is connected with the input of the control unit, and the control unit is electrically connected with the feedback unit; at least one of the linear amplification circuit, the signal processing unit and the control unit is coated with an electromagnetic shielding cover; the number of the pickup units is multiple, the number of the corresponding linear amplification circuits is consistent with that of the pickup units, and any pickup unit is independently connected with one linear amplification circuit. Compared with the prior art, the system is suitable for various scenes of mechanical noise and electric power noise, is good in anti-interference performance, and can provide good platform support for fault analysis, guidance and control.

The application discloses a kind of amplitude-phase grouping modulation-oriented received signal reconstruction methods, it is applicable to communication system receiving end, the amplitude-phase grouping modulation signal of distortion caused by power amplifier nonlinear amplification is reconstructed.The method is first to the frequency domain equalization of received signal, and eliminate the influence of power amplifier linear amplification;Then, the phase of received signal is adjusted, and demodulates additional phase;Subsequently, the amplitude of received signal is revised;Then, based on least square criterion, signal reconstruction optimization problem model is constructed, and least square optimization reconstruction is executed.Afterwards, additional phase demodulation, amplitude adjustment and least square optimization reconstruction are iteratively executed until the preset iteration number is reached, so as to obtain high-quality reconstructed signal.The application can effectively deal with nonlinear distortion at the receiving end of the communication system, realize high-quality signal reconstruction, thereby significantly improving the overall performance and power efficiency of the communication system.

The invention discloses a mass spectrometer linear amplification performance detection method, a mass spectrometer and a storage medium, and relates to the technical field of mass spectrometers. The method comprises the following steps: conveying a standard sample solution with a target concentration to an ion source for ionization; controlling the preceding-stage ion transmission module to start an ion enrichment function, and controlling the mass spectrometer to work under a plurality of different resolutions through the multi-stage ion transmission module; aiming at each resolution ratio, controlling the last-stage ion transmission module to start an ion trap function, enriching and releasing ions from the preceding-stage ion transmission module for multiple times, and acquiring the signal intensity output by the electronic detection module when the ions are released each time, and taking the maximum value of the signal intensity which is linearly increased along with the enrichment time under all the resolutions as the upper limit value of the linear amplification range of the mass spectrometer. Therefore, the upper limit of the linear amplification range of the mass spectrometer can be accurately measured under the condition close to a real signal through multi-stage ion controllable enrichment and release in combination with resolution adjustment.

The invention discloses a temperature control circuit of a self-adaptive heating and cooling constant temperature crystal oscillator, which is characterized in that on the basis of a traditional thermosensitive bridge and a heating circuit, a switch driving mode of a driving circuit is changed into a linear mode, that is, a linear voltage comparison circuit is added, and the linear voltage comparison circuit not only has a linear amplification function, but also has a PID (Proportion Integration Differentiation) calculation function. When the thermo-sensitive bridge is unbalanced, the thermo-sensitive bridge generates a voltage signal difference related to the temperature, the voltage difference is linearly amplified through the linear voltage comparison circuit, and after PID calculation, a thermoelectric cooler TEC of the heating / cooling circuit is driven to heat or cool a circuit board where the thermo-sensitive bridge is located. The linear comparison circuit is used for regulating and controlling the voltage difference and then controlling the heating / cooling circuit, the problem that temperature control fails when the external environment temperature exceeds the set temperature can be effectively solved, control is not in an on state or an off state any more and is related to the amplitude of the voltage difference, and therefore the temperature control precision is improved, and the temperature control efficiency is improved. And the temperature overshoot phenomenon is well inhibited.

The application discloses a wind field prediction method and system based on big data, and the method comprises wind field data acquisition, wind speed interval characteristic quantization, wind field prediction loss framework construction, wind field prediction model establishment and real-time wind field prediction. The application belongs to the field of data processing, and specifically refers to a wind field prediction method and system based on big data. The scheme defines interval weight, dynamically balances the differences between different intervals, defines interval prediction error sensitivity index, designs error sensitivity enhancement factor, and strengthens the feature expression of the core interval. By defining the relative error of wind field prediction, the error sensitivity enhancement factor is introduced to realize the non-linear amplification of the error of the high error sensitivity interval, and to provide a basis for subsequent loss enhancement. A unified loss framework is designed to realize the double mechanism of error amplification and loss enhancement, avoid excessive calibration or insufficient calibration, solve the prediction deviation pain point caused by high wind speed interval error, and further improve the wind field prediction effect.

The invention discloses a broadband dual-polarization high-dynamic transceiving front-end SiP module which comprises a power amplification and transceiving polarization control unit, an amplitude limiting unit, a receiving amplification blocking unit, a ceramic packaging substrate and a heat dissipation carrier. The power amplification and transmit-receive polarization control unit comprises a power amplifier, a transmit-receive switch and a polarization switch; the amplitude limiting unit can bear high-power signal input, limits the amplitude of an output signal under the condition of large signal input, and keeps a low insertion loss characteristic under the condition of normal small signal input; the receiving, amplifying and blocking unit is used for performing linear amplification and programmable attenuation on the amplitude of a received signal, selecting a reference state under the condition of normal small signal input, and selecting a blocking attenuation state under the condition of large signal input; the heat dissipation carrier is embedded in the ceramic packaging substrate and is used for shortening the heat dissipation path of the high-power device. According to the invention, the multi-mode and high-dynamic range of the transceiving front end can be realized, the heat dissipation efficiency of the module is improved, and broadband, miniaturized and high-power output is realized.

The utility model discloses a signal amplification circuit and a temperature detection circuit, the signal amplification circuit comprises a signal acquisition module and a signal amplification module, the output end of the signal acquisition module is connected with the input end of the signal amplification module; the signal acquisition module acquires and processes a to-be-amplified signal source and outputs the acquired to-be-amplified signal to the signal amplification module; and the signal amplification module carries out subtraction processing on the signal to be amplified and the reference signal, carries out linear amplification processing on the signal obtained by signal subtraction, and outputs the signal to an external circuit. Through subtraction processing and linear amplification processing of the signal amplification module, the error of a signal processing result is effectively reduced, and the measurement accuracy is improved.

The application discloses a power supply of a broadband envelope tracking power amplifier, comprising a linear amplification circuit, a comparison circuit and a switching amplification circuit; the linear amplification circuit comprises a linear amplifier, a switching amplifier two and an envelope bandwidth processing unit, the input end of the envelope bandwidth processing unit is used for accessing an envelope, the output end of the envelope bandwidth processing unit is connected with the input end of the switching amplifier two, the output end of the switching amplifier two is used for providing a voltage signal to the linear amplifier, and the input end of the linear amplifier is used for accessing the envelope; the output end of the linear amplifier is connected with the input end of the comparison circuit, the output end of the comparison circuit is connected with the input end of the switching amplification circuit, and the output end of the linear amplifier and the output end of the switching amplification circuit are used for providing a power supply signal to the broadband envelope tracking power amplifier. The application changes the design of the linear power supply, improves the efficiency of the linear power supply at the peak envelope, and thus can improve the overall efficiency of the envelope tracking power supply without reducing the performance.

The disclosed semiconductor amplifier circuit addresses distortion reduction in audio signal amplification by incorporating semiconductor devices with substrate loss pickup elements. The circuit includes a differential amplifier setup using semiconductor devices for initial signal processing and a cascode stage with semiconductor devices equipped with substrate loss pickup elements for mitigating substrate loss currents. Bias networks set operating points for linear amplification, complemented by a power supply connection for operational voltage. Substrate loss pickup elements are configured to reduce distortion by capturing uncollected charge carriers, enhancing audio fidelity.

The utility model discloses a differential combination T mixer for LNP preparation, which belongs to the field of micro-fluidic mixers for small-scale test, pilot-scale test amplification and production of LNP preparation and comprises a central connecting module, a connecting bolt and a liquid inlet and outlet module. According to the differential combination T mixer, the pipe diameter sizes and lengths of three different flow paths can be configured according to the flow velocity of a two-phase solution and the total flow velocity, compared with a traditional T mixer, the standby pressure of the whole system can be reduced, and the production speed and efficiency are improved; in the step-by-step amplification process, liquid inlet and outlet modules are flexibly switched, the Reynolds coefficient or standby pressure of each flow path is kept unchanged, and rapid LNP linear amplification is achieved. And more flexible selection can be carried out according to batch, flow velocity, repeated use or not and the like, the whole body can be made of metal materials (such as stainless steel, titanium alloy, hastelloy and the like) or plastic materials (PEEK, PTFE and the like), and different production requirements can be flexibly met.

The invention provides a super-continuum spectrum light source system based on titanium sapphire laser driving, and relates to the field of biomedical optics and spectral imaging. A three-stage amplification structure is designed to carry out linear amplification on a laser source, the laser bandwidth is effectively reduced while the power is ensured, and the monochromaticity of laser is improved. According to the invention, the annular structure formed by connecting the photonic crystal fiber and the optical fiber coupler is used for widening the visible super-continuum spectrum, the laser can be continuously broadened for multiple times, the spectrum flatness is high, the quantum loss is smaller, and the good output power can be ensured. According to the invention, wavelength switching and power control are realized by using the electronic control AOTF, accurate synchronization with galvanometer scanning and detector acquisition can be realized, the switching speed of the AOTF is at a microsecond level, multi-band excitation can be realized in one scanning process, and multi-channel confocal imaging and spectrum scanning are supported.

The application relates to the technical field of optical communication, and discloses a device and a method for data alignment based on half-rate over-sampling clock, which comprises a transmitting end, an analog front end and an over-sampling data clock recovery end; the transmitting end is used for receiving a plurality of low-speed parallel data signals and clock signals, and performing multiplexing conversion, voltage driving and electro-optical modulation to obtain a first optical signal and a second optical signal; the analog front end is used for performing photoelectric conversion and linear amplification on the first optical signal and the second optical signal to obtain recovered data signals and recovered clock signals; and the over-sampling data clock recovery end is used for performing six-phase clock generation, dense half-rate over-sampling and digital phase judgment on the recovered data signals and the recovered clock signals to obtain an optimal sampling clock and aligned recovered data. Through the combination of the half-rate over-sampling and the digital phase judgment mechanism, the adverse effects caused by transmission disturbance can be reduced, the clock sampling data capacity can be improved, and the overall operation stability of the optical communication link can be improved.

The invention discloses a kit for sensing and detecting a miRNA (micro Ribonucleic Acid) marker for mastitis and a sensing and detecting method. The invention develops a two-pot isothermal miRNA sensing detection method based on RCA amplification and CRISPR / Cas12a, according to the method, isothermal linear amplification of target miRNA is realized by using a lock-type probe mediated RCA technology, HRP on MNP-ssDNA-HRP is released by combining trans-shear activity of CRISPR / Cas12a, and visual color development reading of the miRNA level is realized by using HRP to catalyze TMB / H2O2 color development.

The invention provides a photoelectric sensor detection method and device based on a current difference ratio and a printer, and relates to the technical field of photoelectric sensor detection. According to the method, a sampling resistor network is dynamically gated to ensure that a receiving tube of a photoelectric sensor always works in a linear amplification area so as to obtain reliable voltage signals; and the reference current, the dark current and the bright current under different consumable states and transmitting tube switching states are calculated according to the reference current, the dark current and the bright current. A current difference ratio and a preset judgment threshold value are introduced to serve as judgment bases, and the consumable condition is determined; the ratio effectively counteracts the influence of the ambient light component and the parameter drift of the sensor, and is only related to the reflectivity and the refractive index of the consumable in different states. According to the method, the judgment condition is simple and fixed, the detection accuracy and reliability are remarkably improved, the method has good software and hardware adaptability, and faults such as mark skipping, content dislocation and paper jam are effectively avoided.

The invention discloses an MALBAC linear amplification method and an application thereof. DNA polymerase of the MALBAC linear amplification is Deep, a reaction buffer solution of the Deep contains Mg < 2 + >, and Mg < 2 + > is not additionally added into a reaction system of the MALBAC linear amplification. According to the method, the technical problem of amplification preference in the traditional amplification technology is effectively solved, the detection data quality is high, and the detection accuracy and the result credibility of the newborn RNA molecule in the single cell are remarkably improved. The method is suitable for the field of single cell transcriptomics research, and provides an effective technical means for realizing high-precision and high-reliability single cell gene expression analysis.