The invention belongs to the field of steel material, in particular to a preparation method for forming Ti2O3 nano particles in molten steel. The method has the following characteristics that: Fe-Ti alloy wires are added in molten low-carbon steel or low-carbon alloy steel with the oxygen concentration of less than 100PPm, so that molten steel containing O and Ti is obtained; since the alloying elements Ti and O of precipitated phase Ti2O3 higher than the melting point of matrix alloy are contained, the solubility of Ti and O reduces with the decrease of the temperature, the high cooling speed can form high supercooling degree, meanwhile, the melt can form the high linear rate of flow in the process of solidification, and thereby solidified alloy strengthened by dispersed and precipitated nano Ti2O3 is obtained. The invention has the following advantages that: nano Ti2O3 dispersed phase can be directly formed in the solidified alloy, and thereby the strength of the alloy is enhanced under the premise that the plasticity and toughness of the alloy are nearly kept unchanged.