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898 results about "Sample sequence" patented technology

DNA sequence detection by limited primer extension

A novel limited primer extension reaction improves detection sensitivity and specificity in a variety of hybridization platforms. In the invention, a sequence of target DNA that lacks one of the four types of nucleic acid bases for a span of eight or more adjacent nucleotide positions is selected for use. This sequence is referred to as the extension complement sequence, or ECS. A primer with a sequence that is complementary to the target sequence that is immediately downstream (to the 3′ side) of this ECS is used to initiate an extension reaction. Extension occurs using a DNA polymerase and standard deoxynucleoside triphosphates for three of the four types of nucleic acid bases. The fourth base, which is complementary to the base missing in the ECS, is either absent or present only in the form of a dideoxynucleoside triphosphate, which does not support further extension. In either case, the extension reaction does not proceed past the first occurrence in the template of the base that is missing in the ECS. This results in a primer extension product with fixed length determined by the length of the ECS. The process can be repeated using a thermal-stable polymerase in a thermal-cycled reaction that results in a linear amplification of the targeted sequence. The resulting limited primer extension products serve as ideal hybridization analytes for determination of sample sequence content using microarrays.
Owner:ATOM SCI

Synchronizing clocks across a communication link

Apparatus, system and method for synchronizing one or more clocks across a communication link. A slave clock may be synchronized to a master clock by means of a synchronization signal sent from the master to the slave clock side of the link. The synchronization signal may be an expected signal pattern sent at intervals expected by the slave side. The slave clock may correlate received signals with a representation of the expected synchronization signal to produce a correlation sample sequence at a first sample rate which is related as n times the slave clock rate. The synchronization signal receipt time indicated by the correlation sample sequence may be refined by interpolating the correlation sample sequence around a best correlation sample to locate a best interpolation at an interpolation resolution smaller than the sample resolution. The best interpolation may in turn be further refined by estimating between interpolator outputs adjacent to the best interpolation output. The synchronization signal receipt time thus determined is compared to the expected time based upon the slave clock, which is adjusted until the times match. After initialization, all slave clock errors are preferably accumulated to prevent long-term slip between the slave and master clocks. Formerly independent master and slave clocks synchronized across the communication link constitute a noncommon clock which may be compared on each side of the link to secondary independent clocks, and the secondary independent clocks may then be separately synchronized by adjusting one to have the same difference from its local noncommon clock as the secondary clock on the other side of the link has from its local noncommon clock.
Owner:WI LAN INC

Intelligent fault classification and location method for ultra-high voltage direct current transmission line

The invention discloses an intelligent fault classification and location method for an ultra-high voltage direct current transmission line, and belongs to the technical field of relay protection of power systems. The method comprises the following steps of: classifying fault data by using a neural network by adopting a layered and distributed neural network model; distinguishing fault types; sending the classified data into different neural networks respectively for performing fault location; when the direct current transmission line has a fault and a sampling frequency is 10 kHz, selecting a discrete line mode voltage signal which has the sampling sequence length of 100 after the fault and performing S-transform, wherein a transform result is a complex time-frequency matrix of 51*100; solving the modulus of each element in the complex matrix to obtain transient energy distribution of the line mode voltage at all frequencies; selecting first five energy spectrums as sample properties; selecting a transfer function and a learning rule; setting proper neural network parameters for constructing a BP network model; and performing fault classification and fault location. A large number of simulation results show that the method has a good effect.
Owner:KUNMING UNIV OF SCI & TECH

Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing

InactiveUS6418382B2Rapid and economical and quantitative and precise determination and classificationSufficient discrimination and resolutionData processing applicationsMicrobiological testing/measurementSample sequenceSingle sequence
This invention provides methods by which biologically derived DNA sequences in a mixed sample or in an arrayed single sequence clone can be determined and classified without sequencing. The methods make use of information on the presence of carefully chosen target subsequences, typically of length from 4 to 8 base pairs, and preferably the length between target subsequences in a sample DNA sequence together with DNA sequence databases containing lists of sequences likely to be present in the sample to determine a sample sequence. The preferred method uses restriction endonucleases to recognize target subsequences and cut the sample sequence. Then carefully chosen recognition moieties are ligated to the cut fragments, the fragments amplified, and the experimental observation made. Polymerase chain reaction (PCR) is the preferred method of amplification. Another embodiment of the invention uses information on the presence or absence of carefully chosen target subsequences in a single sequence clone together with DNA sequence databases to determine the clone sequence. Computer implemented methods are provided to analyze the experimental results and to determine the sample sequences in question and to carefully choose target subsequences in order that experiments yield a maximum amount of information.
Owner:CURAGEN CORP

Personnel detection and counting system based on millimeter wave radar

The invention provides a personnel detection and counting system based on a millimeter wave radar, wherein the millimeter wave radar is installed at a set position of a monitoring area, the millimeterwave radar transmits millimeter wave band radio frequency signals to the monitoring area through a multi-transmitting and multi-receiving antenna of the millimeter wave radar and receives echo signals at the same time, the echo signals are subjected to frequency mixing with the transmitted signals and then subjected to down-conversion to obtain beat intermediate frequency signals, and the beat intermediate frequency signals are sampled to obtain an echo sampling sequence. A digital signal processor reads the echo sampling sequence and performs signal processing to obtain a detected point cloud data set. An ARM processor reads the point cloud data set, meanwhile, Kalman filtering tracking is carried out on the point cloud data, continuous observation of states and the number of a pluralityof human body moving targets is achieved, the positions and the number of people in the monitoring area are obtained, and the number of people in the monitoring area is counted. The personnel detection and counting system based on the millimeter wave radar can track at least 20 targets simultaneously, provide the position and speed information of the targets, effectively judge the state of the targets and give early warning in time.
Owner:长沙莫之比智能科技有限公司

Radial basis function (RBF) neural network parameter self-optimizing-based multi-step prediction method for water quality

InactiveCN102737288AImprove the performance of multi-step forecastingEfficient intelligent automatic early warningBiological neural network modelsForecastingSample waterSample sequence
The invention discloses a radial basis function (RBF) neural network parameter self-optimizing-based multi-step prediction method for water quality. The method comprises the following steps of: first storing the data of each monitoring station into a database of a local server by using the remote transmission of an online water quality monitoring instrument; then performing normalization processing on a water quality sample sequence, calculating an autocorrelation coefficient to determine an input variable of an RBF neural network, and converting sample data into a standard dynamic sequence data format trained and predicted by the RBF neutral network; next searching for and determining an optimal value of a spreading coefficient spread of the RBF neural network by utilizing a differential evolution algorithm and taking a relative standard error as a target function to obtain an optimal prediction model; and finally sampling water quality data in real time, performing multi-step prediction by using the obtained optimal prediction model and adopting a single-point iteration method, and evaluating a water quality prediction result to realize an early warning function. The water quality can be intelligently warned.
Owner:ZHEJIANG UNIV

Fault line selection method of distribution network based on empirical mode decomposition (EMD)

The invention relates to a fault line selection method of a distribution network based on empirical mode decomposition (EMD), belonging to the technical field of the relay protection of a power system. The fault line selection method provided by the invention comprises the following steps of: solving a zero-sequence current by utilizing the Clarke transformation theory according to the three-phase current of each line when the distribution network fails and a protection element starts, wherein the three-phase current of each line is measured at the protection mounting part; selecting a discrete zero-sequence current signal with the sampling sequence length of 100 dots, and carrying out EMD (Empirical Mode Decomposition) on the discrete zero-sequence current signal so as to acquire m IMF (Intrinsic Mode Function) components; respectively solving the absolute values of the m IMF components of the zero-sequence current of each line to acquire m absolute values of IMFt (i); and then, summing the m absolute values of IMFt (i) of the zero-sequence current of each line to acquire EMD energy Ei, wherein the line with the maximum Ei value is a fault line. By adopting the sampling frequency of 10 kHz and the time window of 10 ms, the fault line selection method provided by the invention needs data shorter in length, has the advantages of low hardware requirements, easiness in technological implementation, stronger tolerance to transition resistance and stronger practicability and is free from interference influences.
Owner:CHUXIONG POWER SUPPLY BUREAU OF YUNNAN POWER GRID CO LTD
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