178 results about "Complex matrix" patented technology

The invention discloses a magnetic nano-enzyme probe and a preparation method thereof, a kit for detecting helicobacter pylori and application of the magnetic nano-enzyme probe, the core of the magnetic nano-enzyme probe is Fe3O4 nano-particles, magnetic enrichment capacity is provided, and interference of a complex matrix in a sample is eliminated; a layer of Pt nano-particles is electrostatically adsorbed through the cationic polymer to serve as a colorimetric interlayer, and a strong colorimetric signal is provided; au (at) Pt for modifying 5, 5 '-dithiobis (2-nitrobenzoic acid) is arranged on the surface layer and is used for providing strong catalytic signals and sites coupled with a large number of specific antibodies; meanwhile, the inner and outer layers of nano enzyme particles can synergistically enhance the catalytic action. The magnetic nano-enzyme probe disclosed by the invention has strong magnetic responsiveness, high colorimetric signal, strong catalytic activity and specific bacterium capturing capacity, realizes rapid enrichment and quantitative analysis of helicobacter pylori in a sample to be detected, eliminates matrix interference in practical application, and has high sensitivity and high specificity. The method has great potential in the aspects of high sensitivity and clinical monitoring of bacterial infection or pollution.

The invention relates to the technical field of liquid-liquid homogeneous microextraction, in particular to a method for microextraction analysis of perfluorooctanoic acid by using a switchable eutectic solvent. Comprising the following steps: step 1, mixing a hydrogen bond acceptor and a hydrogen bond donor according to a set proportion value, and stirring and mixing at a set temperature value to obtain a eutectic solvent; step 2, filtering the water sample through a water-based filter membrane, then adding a set amount of deep eutectic solvent, dropwise adding an alkaline solution, carrying out full vortex mixing through a mixer, and then adding an acidic solution to form an emulsion; according to the method, the high selectivity of the green solvent with a specific structure on perfluorooctanoic acid and the characteristic that the solvent is switched between hydrophilic and hydrophobic forms are utilized, so that the microextraction time is shortened, and the impurity removal and enrichment effects are enhanced. The method is simple and convenient to operate, green, environment-friendly, low in cost and good in effect, has the advantage of efficiently and rapidly pretreating the batch small-size complex matrix sample, and has a relatively good application prospect.

The invention relates to a food-borne microorganism detection method and system based on a spectrum technology. The method comprises the following steps: carrying out correlation analysis on the content sequence of each chemical substance in a plurality of blank matrix samples and the absorbance sequence of each wave band in spectral data of the plurality of blank matrix samples to obtain an aliasing coefficient of each wave band; analyzing the content of each strain in the plurality of strain samples and the absorbance of each wave band in the corresponding strain spectral data to obtain an absorbance influence coefficient between any two strains under each wave band; determining a flora representative coefficient of each wave band according to the number of the plurality of strains, the aliasing coefficient of each wave band and the absorbance influence coefficient of other strains on the first strain under the corresponding wave band; and according to the flora representative coefficient of each wave band and the absorbance sequence of the corresponding wave band in the spectral data of the plurality of target samples, screening a characteristic wave band from each wave band. According to the method, the analysis difficulty during coexistence of multiple flora in a complex matrix is simplified, and the generalization ability and the practical application value of the model are improved.

The invention provides a fluorescence probe signal calibration method and system for rapid detection of multiple residues in a complex matrix, and the method comprises the steps: collecting a fluorescence signal of a to-be-detected sample, and analyzing the original concentration, the fluorescence average lifetime and the fluorescence peak emission wavelength of the to-be-detected sample from the fluorescence signal; calculating a life deviation between the fluorescence average life and a preset reference life, and a wavelength deviation between the fluorescence peak emission wavelength and a preset reference wavelength; calculating to obtain a signal fidelity index based on the life deviation and the wavelength deviation; according to a preset threshold interval in which the signal fidelity index falls, a corresponding calibration strategy is determined, and the calibration strategy is one of non-calibration, calibration execution or invalid marking; and when the calibration strategy is to execute calibration, calculating a total calibration factor based on the life deviation and the wavelength deviation, calibrating the original concentration by using the total calibration factor, and outputting the calibrated concentration.

The invention discloses a compound monitoring method and system based on chromatographic column analysis and a medium, and relates to the technical field of chromatographic detection.The method comprises the steps that a complex matrix sample is taken, and first chromatographic column response distribution of the complex matrix sample is collected through a diode array detector; enhancement strategy optimization is carried out according to adjustable action parameters of the diode array detector, and the diode array detector collects second chromatographic column response distribution of the complex matrix sample under a first enhancement strategy; identifying a difference chromatographic column response distribution of the first chromatographic column response distribution and the second chromatographic column response distribution, and constructing a high-dimensional feature fusion fingerprint; and performing similarity measurement based on the high-dimensional feature fusion fingerprints and a pre-constructed compound feature fingerprint database to obtain a compound detection result. The technical problem of low detection accuracy caused by insufficient chromatographic response information of the compound under a complex matrix condition in the prior art is solved, and the technical effect of improving the detection accuracy of the compound is achieved.

The invention discloses a Janus-MOF composite material as well as a preparation method and application thereof. The material is formed by heterogeneously integrating an adsorption module and a signal module through a lattice orientation growth method, wherein the adsorption module adopts a zirconium-oxygen cluster and a tetrafluoroterephthalic acid ligand to construct a molecular imprinting pore channel to specifically capture PFAS; the signal module comprises a spiropyrane modified ligand and an electrochemically deposited gold nano needle tip array, and the SERS detection limit reaches 0.05 ppt through photoresponse aperture regulation and three-mode (electromagnetic-chemical-mechanical) synergistic enhancement; the shell layer is doped with titanium dioxide quantum dots, PFAS can be degraded under ultraviolet irradiation, and the substrate can be recycled for more than or equal to 5 times. The invention further provides a surface enhanced Raman spectroscopy (SERS) and electrochemical impedance spectroscopy (EIS) dual-mode coupling technology, the cross validation detection error is less than or equal to 15%, the problems that the traditional method is poor in selectivity, low in sensitivity and incapable of in-situ regeneration are solved, and the method is suitable for rapid detection of trace PFAS in complex matrixes such as food, surface (undersurface) water and industrial wastewater.

The invention discloses an ICP-MS (Inductively Coupled Plasma Mass Spectrometry) detection method for phosphorus in trichlorosilane, and belongs to the field of analysis of high-purity electronic chemicals. According to the invention, a low-temperature matrix digestion technology is adopted to quantitatively form a SiFcomplex and inhibit silicon volatilization loss; according to the matrix matching calibration system, a calibration curve is constructed by adopting a weighted least square method, so that matrix interference is effectively corrected; a detection technology screening strategy is carried out by combining a cascade strategy of high-resolution inductively coupled plasma mass spectrometry, so that the interference of a complex matrix is successfully avoided. Experiments show that when the method is used for detecting phosphorus, the linear correlation coefficient R is greater than 0.999, the detection limit is as low as 0.02 ng / g, and the method accords with ISO 17025 and semiconductor-grade material quality control standards, and is suitable for accurate quantitative analysis of ng / g-grade phosphorus elements in high-purity trichlorosilane.

The invention belongs to the technical field of biological detection, and particularly relates to a specific monoclonal antibody for AKK bacterium active protein P9, a detection kit and application of the specific monoclonal antibody. The antibody pair is 7G4 and 8B2, the amino acid sequence of a heavy chain variable region of the 7G4 is SEQ ID NO.2, and the amino acid sequence of a light chain variable region of the 7G4 is SEQ ID NO.4; the amino acid sequence of the 8B2 heavy chain variable region is SEQ ID NO.6, and the amino acid sequence of the 8B2 light chain variable region is SEQ ID NO.8. The antibody pair has strong specificity and high affinity, and can accurately recognize different antigen epitopes of the P9 protein. A colloidal gold detection and double-antibody sandwich ELISA detection system constructed based on the kit adapts to a complex matrix and various process treatment samples, is stable and reliable in detection, has qualitative and quantitative capabilities, can be used for scenes of P9 protein detection, fermentation monitoring, quality control and the like, and solves the problems of insufficient specificity, poor adaptability and the like in the prior art.

The invention relates to the technical field of environmental and biological analysis and detection, in particular to a method for extracting perfluorinated / polyfluorinated compounds based on confinement fluid. The method comprises the following steps: providing a multi-dimensional reticular nanopore material; growing mutually staggered carbon nanofibers on the surface of a substrate through a chemical vapor deposition method; loading a confinement fluid in a pore channel of the multi-dimensional reticular nano-pore channel material; contacting the material with a to-be-detected sample, and extracting; desorbing the extracted material by using a desorption solvent to obtain a desorption solution; and detecting the perfluorinated / polyfluorinated compounds in the desorption solution. A multi-dimensional net-shaped nano pore channel formed by mutual staggering of the carbon nanofibers provides a stable multi-dimensional micro-nano confinement space for confinement fluid. According to the structure, mass transfer and distribution of a target analyte from a complex matrix to a confinement fluid are greatly promoted, so that efficient enrichment of various perfluoro / polyfluoro compounds is realized, and the recovery rate and the detection sensitivity of the method are improved.

The present invention discloses a method for quantitative determination of trace harmful elements in various flotation products of rare polymetallic ores, which includes three parts: sample processing, preparation of complex matrix standard solution and sample testing. Each flotation product is digested with analytically pure concentrated nitric acid; the main mineral species of each product are determined by XRD, and the high-purity standard substances of each main associated mineral are digested with analytically pure concentrated nitric acid, and the content of harmful elements to be monitored is determined by ICP-MS after appropriate dilution; each standard substance digestion solution is diluted to a major element concentration of 1000μg / mL, and used to dilute the harmful element standard stock solution to obtain standard solutions with different concentration gradients; then the process blank and digestion sample are tested using an inductively coupled plasma mass spectrometer using a combination of internal standard method and matrix matching to obtain the content of trace harmful elements to be measured. It effectively overcomes the mass spectrometry and non-mass spectrometry interference problems faced by complex matrix sample testing, is suitable for the analysis and testing of trace element content in complex samples, and has a simple method and is easy to operate.

This invention discloses a pretreatment method for detecting trace perfluorinated and polyfluoroalkyl substances (PFAS) in complex matrix textiles, belonging to the field of materials analysis technology. The method involves placing the textile in an alkaline solvent containing an alkyl sulfonate displacing agent. Under heating conditions, the displacing agent utilizes its competitive adsorption mechanism to forcibly occupy matrix adsorption sites and displace the target analytes into the solvent. Subsequently, the temperature is rapidly lowered to the phase separation induction temperature, inducing supersaturation precipitation of the displacing agent, which then encapsulates impurities in situ and co-precipitates, forming a solid-liquid stratified system. Finally, the supernatant is collected for analysis. This invention utilizes the dual properties of the displacing agent—high-temperature solubilization and low-temperature phase change removal—to effectively break the strong adsorption equilibrium of aged textiles without using a solid-phase extraction column. This solves the contradiction between low extraction rate and significant matrix interference, significantly improving the recovery rate of PFAS. It offers advantages such as low cost, simple operation, and high accuracy.

The invention relates to the technical field of biochemistry, and discloses a DNA chimera preparation and application method, which comprises the following steps: synthesizing phosphorylated linear annular L-rTDNA and template DNA, mixing in a 1 * T4 DNA ligase buffer solution according to an equal molar concentration of 10 [mu] M, heating at 95 DEG C for 5 minutes, and then carrying out gradient cooling to room temperature for renaturation for 120 minutes; adding T4DNA ligase to close a tail end notch, and carrying out enzyme digestion treatment to obtain annular rTDNA; the method comprises the following steps: synthesizing hairpin DNAHP (deoxyribonucleic acid), mixing rTDNA and HP according to an equal molar concentration of 2 mu mol / L, denaturating at 95 DEG C for 5 minutes, slowly cooling to 25 DEG C within 2 hours, and self-assembling to obtain the DNA topology limited chimera. According to the invention, a DNA chimera with dynamic response capability is constructed, an integrated detection process of enzyme-free assembly-target triggering-signal cascade amplification is realized, and meanwhile, the problems of anti-interference and ultra-sensitive detection in a complex matrix are solved.

The invention relates to the technical field of low earth orbit (LEO) satellite communication, in particular to a deep expansion large-scale MIMO LEO satellite precoding method based on a graph neural network (GNN). The method comprises the following steps: firstly, constructing a power-limited energy efficiency maximization precoding problem of large-scale MIMO LEO satellite communication; secondly, converting an original problem into a series of iterative solution sub-problems through a Dinkelbach algorithm, and converting the sub-problems into convex problems by using a weighted minimum mean square error (WMMSE) method to obtain an iterative update expression of a user precoding vector; the GNN is constructed for the complex matrix inversion in the user precoding vector updating process to perform depth expansion, and the Taylor expansion is applied to approach the matrix inversion operation in the GNN framework, so that the calculation efficiency is further improved. According to the method, higher energy efficiency of the system can be realized, the complexity of solving the optimization problem is remarkably reduced, and the operation speed is increased.

The invention discloses a data processing method, the method is applied to data processing equipment, and a processor architecture of the data processing equipment at least comprises a universal central processing unit (CPU) and a plurality of matrix acceleration modules. The method comprises the following steps: reading a real part data set and an imaginary part data set of a first data type stored in a first register in the complex matrix acceleration module; wherein the real part data set and the imaginary part data set correspond to a preset number of matrix data blocks; performing multiply-accumulate operation on the real part data set and the imaginary part data set through a first calculation unit corresponding to the first data type in the complex matrix acceleration module to obtain a first calculation result; and storing the first calculation result to a first storage area corresponding to the complex matrix acceleration module. The invention further discloses a data processing device and equipment, a storage medium and a computer program product.

The invention discloses a near-zero background fluorescence sensor based on polydopamine functionalized MXene as well as a preparation method and application of the near-zero background fluorescence sensor, and belongs to the technical field of universal biosensing. The sensor comprises a polydopamine functionalized MXene material and a quantum dot-aptamer fluorescent probe which is accurately coupled by click chemistry in a ratio of 1: 1. The detection method is characterized in that a target object is utilized to induce the probe to desorb from the surface of a material, and a supernatant containing the free probe is physically separated from a material precipitate through centrifugal separation, so that background fluorescence interference is almost completely eliminated. According to the invention, ultra-sensitive detection of aflatoxin B1 is realized, and the detection limit of AFB1 in complex matrixes such as corn flour is as low as 0.27 mu g / kg. All components of the platform can be modularly and independently stored for a long time, the platform can be directly applied to detection of various micromolecular mycotoxins without optimization by replacing aptamers, and the platform has high sensitivity, high stability and high universality.

The application relates to the technical field of automatic charging, and provides a control method and system of an automatic charging device, a mechanical arm of the automatic charging device comprising three rotary joints, one translation joint and joint arms between the joints, the rotary planes of the three rotary joints being parallel to each other, the translation joint driving a charging gun to move along a translation axis, the method comprising the following steps: acquiring current pose parameters of the mechanical arm and a target pose of the charging gun; calculating a current pose of the charging gun based on the current pose parameters; and planning a joint path of the mechanical arm based on a two-dimensional search method and the geometric relationship between the rotation angle of the charging gun relative to the translation axis and the joint angles of the rotary joints according to the current pose and the target pose. The application does not need to establish a D-H kinematics model and does not need to perform complex matrix operations; moreover, the two-dimensional search method can directly obtain an optimal solution without iterative calculation, reduces the calculation time cost, and has high overall planning efficiency.

The invention belongs to the technical field of environmental analytical chemistry, and particularly relates to a separation and purification and high-throughput target quantitative analysis method for multiple types of environmental pollutants in a complex matrix biological sample. The method comprises the following specific steps: S1, carrying out ultrasonic extraction on a biological sample to obtain an extracting solution; s2, performing dispersive solid-phase extraction and purification treatment on the extracting solution to obtain a solution to be detected; s3, determining the environmental pollutants in the liquid to be detected by adopting a selected ion scanning mode of the gas chromatograph-mass spectrometer. The pretreatment method disclosed by the invention can effectively reduce complex matrix interference of the biological sample, and can quickly and efficiently perform high-throughput target analysis on multiple types of environmental pollutants in the biological sample; the method disclosed by the invention is low in detection limit and capable of realizing trace detection of a biological sample; the invention provides a new method for researching the occurrence condition of the environmental pollutants in the 17 marine organisms in Liaoning Bay, and has important significance for the progress of environmental science.

Aiming at the problem that high-sensitivity and high-accuracy quantitative analysis is difficult to realize when a traditional detection method is used for a complex multi-component mixture, the invention discloses a terahertz spectrum quantitative analysis method for a rubber mixture. Based on a terahertz spectrum technology and in combination with an improved rhodeus ocellatus optimization algorithm (IFBO), accurate detection of the content of two trace anti-aging agents (NBC and 44S) in a five-component rubber mixture is realized. Through systematic experimental design and spectral analysis, differentiation characteristics of different anti-aging agent proportions in a time domain and an absorbance spectrum are determined, and SG preprocessing, PCA and SPXY data set division methods are utilized, so that the signal-to-noise ratio of spectral data and the model generalization ability are effectively improved. A support vector regression (SVR) model is introduced according to small-sample and high-dimensional nonlinear spectral data characteristics, and the significant advantages of IFBO in parameter optimization are verified by comparing the optimization effects of GA, PSO and FBO. Experimental results show that the correlation coefficient (Rp) of the IFBO-SVR model on a prediction set reaches 0.9879, the root mean square error (RMSEP) is reduced to 0.0024, and compared with a traditional algorithm, the method has higher accuracy and stability. The invention not only provides an efficient technical scheme for rapid detection of trace anti-aging agents in complex matrixes, but also lays a theoretical foundation and basis for quality control of rubber products and environmental safety assessment.

The invention discloses an attapulgite-multi-walled carbon nanotube purification material as well as a preparation method and application thereof. The attapulgite-multi-walled carbon nanotube is used as a purifying agent in tea pesticide detection, matrix interferents can be effectively removed, the surface area of the attapulgite-multi-walled carbon nanotube is large, and pigments can be adsorbed from a complex matrix through the electron interaction and hydrophobic interaction of the multi-walled carbon nanotube; the porous structure of the attapulgite can physically adsorb and capture macromolecular impurities (such as proteins and polysaccharides), and amido bonds on the interface of the attapulgite-multi-walled carbon nanotubes can also adsorb polar molecules (such as organic acids). The attapulgite-multi-walled carbon nanotube interface is connected through a chemical bond, so that the phase separation problem of a physical mixed adsorbent is avoided, the material is uniformly dispersed in a solvent and can be in rapid contact with a matrix, and the purification efficiency is improved. Compared with a traditional QuEChERS method, the method has the advantages that a single material attapulgite-multiwalled carbon nanotube is adopted as a purifying agent, matrix impurities can be efficiently removed without multi-material combination, and the method is small in dosage, high in adsorption capacity, simple in experimental operation and low in raw material cost.

This invention discloses a magnetic covalent organic framework composite material modified with nucleic acid aptamers, its preparation method, and its applications. The composite material uses magnetic iron oxide microspheres as the core, with the surface of the core coated with a shell of covalent organic framework material. Nucleic acid aptamers capable of specifically recognizing tetracycline antibiotics are stably assembled onto the shell surface through non-covalent interactions. This invention fully utilizes the large specific surface area and electron-rich framework of magnetic covalent organic framework materials as efficient carriers for nucleic acid aptamers, effectively overcoming the shortcomings of traditional adsorption materials in terms of poor selectivity and limited adsorption capacity in complex matrices, while avoiding cumbersome covalent modification processes. This composite material exhibits excellent adsorption capacity and superior selectivity for tetracycline antibiotics, and is particularly suitable for rapid and efficient magnetic solid-phase extraction of trace tetracycline antibiotics in complex animal-derived food matrices, significantly improving the detection sensitivity and accuracy of subsequent instrumental analysis.

The invention discloses a Pseudomonas fragi aptamer as well as a screening method and application of the Pseudomonas fragi aptamer, and relates to the technical field of microorganisms. The DNA (Deoxyribose Nucleic Acid) sequence of the Pseudomonas fragi aptamer is as shown in SEQ ID No: 1 (Sequence Identifier Number 1). The parameter dynamic mapping and iteration mechanism of a multi-objective evolutionary algorithm model is adopted, the OmpA membrane protein is obtained through molecular docking and multi-objective evolutionary algorithm optimization screening, the OmpA membrane protein can be specifically bound with pseudomonas, the binding rate of the OmpA membrane protein with pseudomonas is as high as 79.2%, and the OmpA membrane protein has high specificity and sensitivity. Even if in a complex matrix containing high protein, the detection error rate can be as low as 8%, which indicates that the aptamer can successfully inhibit environmental interference, can effectively avoid background interference, and can improve the detection accuracy.

The invention belongs to the technical field of analysis and detection, and particularly relates to a multifunctional magnetic adsorbent and a preparation method and application thereof. According to the preparation method of the multifunctional magnetic adsorbent provided by the invention, the magnetic material, the adsorbent, the pH regulator and the derivative reagent are mixed and ground, and the self-assembly of the material is realized by adopting a one-pot grinding method, so that the multifunctional magnetic adsorbent is prepared. The multifunctional magnetic adsorbent integrates the functions of magnetism, adsorption, pH adjustment and derivation at the same time, when the material is used for sample pretreatment, a pH adjusting agent or a derivation reagent does not need to be additionally added, meanwhile, rapid liquid-solid separation can be achieved with the help of a magnet, centrifugation or filtration is not needed, and therefore operation steps can be effectively simplified, operation errors are reduced, and the operation efficiency is improved. Efficient derivatization and enrichment of a target analyte are achieved, and the method is suitable for high-sensitivity analysis and detection of complex matrix samples and particularly suitable for analysis of small molecular compounds.

The invention relates to a method for recovering organic molecules (10) from a complex matrix (20) said method (100) comprising the steps of: Providing (110) the complex matrix (20); Contacting (120) the complex matrix (20) with biogenic nanoparticles (30), for an adsorption of the organic molecules (10) on biogenic nanoparticles (30); Separating (140) biogenic nanoparticles enriched (35) with the organic molecules (10) from a depleted complex matrix (22); and Using (150) the organic molecules (10) that were adsorbed to the biogenic nanoparticles (30) in a chemical process or in a chemical device.

The invention discloses a rapid detection method for domoic acid based on a carbon nanotube and an aptamer, and belongs to the field of domoic acid detection.The rapid detection method comprises the steps that the amino-modified aptamer is covalently fixed to the surface of the single-walled carbon nanotube in an orientation controllable mode through carboxyl activation-amide bonding, and an integrated conductive sensing element is constructed; after the element is in contact with a sample to be detected, domoic acid is specifically combined with the aptamer to cause instantaneous change of conductance of the carbon nano tube, and a result is read by the micro-bridge circuit and output within 1 minute. The method does not need pretreatment, large-scale instruments and cold chain storage, has strong anti-interference capability on complex matrixes, has a detection limit of 0.02 ng mL and has relative standard deviation of lt; therefore, on-site high-sensitivity rapid screening of domoic acid is realized.

The invention provides a molecularly imprinted material PtCo-MIP for kynurenine detection, a preparation method, application and a product, and belongs to the technical field of analytical chemistry. According to the invention, PtCo alloy is combined with a molecular imprinting technology; wherein the PtCo alloy has more excellent oxidase-like activity compared with Pt nanoparticles, and the molecular imprinting technology solves the problem of insufficient specific detection of a target factor by nano-enzyme by forming a cavity complementary with a kynurenine structure on the surface of the PtCo alloy, so that efficient and safe kynurenine specific recognition is realized, and the detection sensitivity is improved. And the blank of low-cost and high-specificity detection technology in objective diagnosis of depression is filled. The kynurenine detection kit has an extremely wide detection range on kynurenine, and the detection range and the sensitivity are far better than those of an existing commercial kit; meanwhile, the anti-interference capability is excellent, accurate detection can be performed even in a complex matrix, the detection performance is excellent, and an accurate biological basis is provided for early diagnosis of depression.

The invention discloses a multi-WiFi device adaptive selection method for cooperative tracking in a complex environment. The method comprises the steps that when multiple WiFi devices track a target object in a complex environment, CSI data of each antenna of any wifi device is acquired, each wifi device comprises three antennas, and each piece of CSI data is a 3 * 30 complex matrix; based on the CSI data of each antenna of any wifi device, determining a Fisher information matrix of Doppler frequency shift of the CSI data of any wifi device; and based on the Fisher information matrix of the Doppler frequency shift of the CSI data of any wifi device, establishing a relation model between the Doppler frequency shifts of the receivers of all wifi devices and the speed parameters of the target object. The technical problems of low tracking efficiency, large error and poor stability when commercial WiFi equipment tracks a target object in a complex environment in the prior art are solved.

The invention discloses five food-borne pathogenic bacteria LAMP method high-throughput detection primer groups, a kit and application, and belongs to the technical field of food-borne pathogenic bacteria detection. The primer group corresponds to five bacteria, namely salmonella, vibrio parahaemolyticus, listeria monocytogenes, escherichia coli O157: H7 and staphylococcus aureus, and the nucleotide sequences of the primers are SEQ ID No.1-30. A matched system can complete the whole-process detection at the constant temperature of 62 DEG C for 45 minutes, the result does not need a complicated instrument, the positive grey and negative purple are judged by naked eyes, and the operation is simple and convenient; the detection sensitivity reaches 1-75 copies / reaction, an artificially polluted aquatic product sample does not need to be subjected to enrichment and is subjected to simple splitting decomposition and enrichment, the specificity is high, and no cross reaction exists; the anti-interference capability is outstanding, the method adapts to complex matrixes of aquatic products, and the detection coincidence rate is high; the device is simple in structure and low in cost, only needs a conventional constant temperature device, is suitable for basic food, agriculture, disease control, customs and other detection institutions, food enterprises, on-site law enforcement and on-site detection and other scenes, and provides an efficient and economic technical means for aquatic product safety management and control.

The invention discloses a bimetal SERS (Surface Enhanced Raman Scattering) nano probe based on a biological silence region as well as a preparation method and application of the bimetal SERS nano probe. According to the sensor, AuNS (at) 4-MBN (at) Ag-aptamer is adopted as the nano probe, and Fe3O4 (at) AuNPs-cDNA (FA-cDNA) is adopted as a magnetic substrate. In the sensor, an FA-cDNA (complementary deoxyribonucleic acid) and AuNS (at) 4-MBN (at) Ag-aptamer form an FA-cDNA-aptamer-AuNS (at) 4-MBN (at) Ag magnetic complex through a base complementary pairing effect. The SERS sensor established by the bimetal nanoprobe in the'biological silence 'region has important practical application significance in trace CPF detection in a complex matrix environment.

The invention provides a preparation method of a chip for quantitative detection of acyl carnitine in a high-coverage and high-sensitivity clinical sample. The preparation method comprises the following steps: A) preparing a silicon nanowire material; b) performing in-situ reduction modification on the gold nanoparticles by the silicon nanowire material to obtain an Au-SiNWs material; and C) carrying out a self-assembly reaction to prepare the Sulfo-Au-SiNWs material. According to the method, the ionization efficiency is enhanced through the synergistic effect of the introduced surface sulfonic acid groups and the gold nanoparticles, and the signal response of AC in a high-salt complex matrix is effectively improved. In the aspect of quantification, the innovatively designed full-spectrum internal standard method for perdeuterated ethanol derivatization avoids peak overlapping by utilizing isotope quality difference, breaks through the traditional quantification mode depending on a few standard substances, realizes high-coverage and high-accuracy quantitative analysis on AC in a clinical sample for the first time, and remarkably improves the universality and clinical operability of the method.