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This invention provides a kit for detecting the pathogen of turtle mumps disease and its usage method, belonging to the field of biodetection technology. The kit includes a tissue pathogen release buffer, magnetic macroporous SiO2 microspheres, nucleic acid extraction solution, and detection reagents for the turtle mumps disease pathogen. This invention uses the tissue pathogen release buffer to release pathogens from tissues, magnetic macroporous SiO2 microspheres for pathogen enrichment, and nucleic acid extraction reagents to obtain a crude extract that can be directly used for LAMP amplification and qPCR amplification detection.