10results about How to "Qualitatively accurate" patented technology

The invention discloses a kit for detecting HLA-B27 and a detection method. The kit for detecting HLA-B27 comprises a PCR (Polymerase Chain Reaction) solution, a primer probe mixed solution, a positive reference substance and a negative reference substance, the primer probe mixed solution comprises an upstream primer 1F, a downstream primer 1R and a probe 1P for detecting the HLA-B27, and an upstream primer 2F, a downstream primer 2R and a probe 2P for detecting a reference gene. A fluorescent PCR-melting curve method is adopted, sequence alignment is carried out according to an NCBI database, primers and probes which are high in specificity and capable of simultaneously detecting main subtypes (HLA-B27: 02, HLA-B27: 04, HLA-B27: 05, HLA-B27: 06, HLA-B27: 07 and HLA-B27: 24) of the HLA-B27 are selected, and compatibility detection of different subtypes of the HLA-B27 can be achieved through a pair of primers and a probe. The Tm value in the melting curve instead of the Ct value of the amplification curve is adopted for result judgment, qualitative accuracy is achieved, and the result is visual and easy to interpret.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The invention relates to the technical field of chemical analysis, in particular to a method for detecting free carotenoid in chili. The detection method comprises the following steps: detecting the capsicum extract by using ultra-high performance liquid chromatography-tandem mass spectrometry, and the liquid chromatography in the ultra-high performance liquid chromatography-tandem mass spectrometry comprises the following mobile phase components: a mobile phase A: methanol or acetonitrile, formic acid and ammonium formate, the volume percentage of formic acid in the mobile phase A is 0.05-0.2%, the volume percentage of ammonium formate in the mobile phase A is 0.05-0.2%, and the volume percentage of ammonium formate in the mobile phase A is 0.05-0.2%; the concentration of ammonium formate in the mobile phase A is 4.5-5.5 mM; and the mobile phase B is an MTBE (Methyl Tert-Butyl Ether) solution containing 0.005 to 0.02 g / 100mL of antioxidant. The detection method can accurately detect the free carotenoid in the pepper and perform quantitative analysis, and meanwhile, the detection method is simple and rapid to operate, high in sensitivity and good in repeatability.

This invention discloses a method for detecting and analyzing toxic gases based on flame photometry. The method includes: determining the types and quantities of constituent atoms of each toxic gas according to its molecular formula; determining the characteristic elements of each toxic gas, and further determining the typical characteristic values ​​of each toxic gas; each toxic gas uniquely corresponds to a set of typical characteristic values; the set of typical characteristic values ​​is calculated from the types and quantities of atoms corresponding to the characteristic elements; measuring the constituent atoms and content of the gas to be tested using flame photometry, calculating the measured characteristic values ​​of the gas to be tested, and obtaining the set of measured characteristic values ​​of the gas to be tested; comparing the obtained set of measured characteristic values ​​with known sets of typical characteristic values; if the set of measured characteristic values ​​completely corresponds to a certain set of typical characteristic values, then the gas to be tested can be determined to be the toxic gas corresponding to that set of typical characteristic values.

The invention discloses an integrated micro-fluidic chip system for detection, photothermal decomposition and verification of perfluorinated and polyfluoroalkyl substances. The integrated micro-fluidic chip system comprises an upper ultraviolet light-emitting layer, a middle micro-fluidic main body layer and a lower constant-temperature heating layer, the middle microfluidic main body layer internally comprises an inlet channel, an optical detection module, a photothermal decomposition chamber, a circulating pump structure, a residue verification chamber and an outlet channel; the upper ultraviolet light-emitting layer and the lower constant-temperature heating layer are respectively arranged corresponding to the photothermal decomposition chamber so as to provide a light field and a thermal field which have a synergistic effect; the system further comprises an external control circuit for controlling the operation of the circulating pump structure, the upper ultraviolet light-emitting layer and the lower constant-temperature heating layer, and controlling the path selection of the fluid according to the output result of the residual verification chamber, so as to realize a closed-loop treatment flow. According to the three-layer micro-fluidic chip system provided by the invention, efficient and reliable treatment on perfluoro and polyfluoroalkyl substances is realized through photo-thermal synergistic degradation and closed-loop feedback control.

The application provides a non-target detection device and system based on a nanopore and a detection method thereof, the non-target detection device and system based on a nanopore are used for non-target detection of a complex matrix through a nanopore, feature extraction is performed on a nanopore detection graph, and sample classification is realized through model construction, the detection method does not make a prior definition on a to-be-detected target, and various molecular information existing in serum is acquired in a net catching manner, non-target capture and overall state analysis of various molecules in serum are realized, and a new way of rapid and portable disease screening is provided.The application breaks through the limitation of traditional single marker detection by combining the nanopore technology and machine learning, and is expected to promote the development of precision medicine in a more efficient and comprehensive direction.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for determining 4-(4-amino-3-fluorophenoxy) pyridine-2-carboxylic acid methylamine and 2-chloro-5-aminobenzotrifluoride in a medicine, the determination method is a liquid chromatography, a C8 chromatographic column is adopted, a mobile phase A is selected from a phosphate solution, a mobile phase B is selected from a nitrile solvent, and gradient elution is carried out. The detection method has good specificity, repeatability and accuracy and high sensitivity, can effectively and accurately perform qualitative and quantitative analysis on the compound, and ensures safety and effectiveness of medicine quality.

The application discloses biomarkers for atherosclerosis diagnosis and application thereof. The biomarkers for atherosclerosis diagnosis include any one or any combination of PG(38:7), PG(34:2), PG(34:3), PG(38:6), PG(36:6), PG(38:5), PG(36:3) and PG(36:4). The biomarkers provided by the application have a strong correlation with advanced atherosclerotic plaques, and can be used for diagnosis and experimental research of atherosclerosis, especially advanced atherosclerotic plaques.

The invention discloses a detection method for oligomeric isomaltosaccharide content in a formula milk powder, including: S1: preparing hydrochloric acid, sodium hydroxide solution, PBS buffer and beta-galactosidase-glucose oxidase solution; S2: weighing the standard of each sugar respectively, configuring it into standard working solutions of different concentrations; S3: using high performance liquid chromatography, obtaining the peak area values ​​of standard working solutions of different concentrations, and using the peak area value as the ordinate, using the concentration value of the corresponding standard working solution as the abscissa, establishing a standard curve; S4: weighing the sample to be tested, adding beta-galactosidase-glucose oxidase solution to configure it into liquid to be tested; S5: obtaining the peak area value of liquid to be tested by high performance liquid chromatography, checking the concentration of each sugar in the liquid to be tested in the corresponding standard curve according to the peak area value, and then obtaining the content of each sugar in the sample to be tested by conversion formula. The present invention achieves the purpose of accurately detecting the content of oligomeric isomaltosaccharide in formula milk powder.

This invention provides a kit for detecting the pathogen of turtle mumps disease and its usage method, belonging to the field of biodetection technology. The kit includes a tissue pathogen release buffer, magnetic macroporous SiO2 microspheres, nucleic acid extraction solution, and detection reagents for the turtle mumps disease pathogen. This invention uses the tissue pathogen release buffer to release pathogens from tissues, magnetic macroporous SiO2 microspheres for pathogen enrichment, and nucleic acid extraction reagents to obtain a crude extract that can be directly used for LAMP amplification and qPCR amplification detection.