20results about How to "Qualitatively accurate" patented technology

The invention relates to a sample treatment method for selectively removing glycosyl from glycosphingolipid through photocatalysis and a mass spectrometric detection method. The sample treatment method specifically comprises the following steps: (1) dissolving a sample in an alkaline protic solvent; (2) dispersing titanium dioxide particles; and (3) carrying out photocatalytic reaction under ultraviolet irradiation, and centrifuging to obtain a sample solution subjected to photocatalytic treatment. The mass spectrometric detection method specifically comprises the following steps: injecting a sample solution subjected to photocatalysis treatment into a mass spectrum, carrying out primary mass spectrometric detection, and carrying out comparison data analysis on the sample solution subjected to photocatalysis treatment and a sample extracting solution not subjected to photocatalysis treatment, a primary mass spectrum peak which is possibly a glycolipid decomposition product is screened through comparison and analysis results, and secondary mass spectrum detection is further carried out, so that the accurate molecular structure of glycolipid is analyzed. According to the method, the glycosyl head of glycolipid can be selectively, rapidly and efficiently removed in the presence of complex lipidomes, subsequent mass spectrum detection analysis is carried out, glycolipid spectrum peaks in complex mass spectrum peaks can be effectively, rapidly and accurately found out by comparing the mass spectrum peaks before and after removal, and the detection result is accurate. Important chemical information is provided for rapid locking of correct candidate substances in the first-level mass spectrum qualitative process, and meanwhile, the glycolipid decomposition product can obtain carbon chain structure information which is richer than that of original glycolipid to assist qualitative analysis.

The invention discloses a kit for detecting HLA-B27 and a detection method. The kit for detecting HLA-B27 comprises a PCR (Polymerase Chain Reaction) solution, a primer probe mixed solution, a positive reference substance and a negative reference substance, the primer probe mixed solution comprises an upstream primer 1F, a downstream primer 1R and a probe 1P for detecting the HLA-B27, and an upstream primer 2F, a downstream primer 2R and a probe 2P for detecting a reference gene. A fluorescent PCR-melting curve method is adopted, sequence alignment is carried out according to an NCBI database, primers and probes which are high in specificity and capable of simultaneously detecting main subtypes (HLA-B27: 02, HLA-B27: 04, HLA-B27: 05, HLA-B27: 06, HLA-B27: 07 and HLA-B27: 24) of the HLA-B27 are selected, and compatibility detection of different subtypes of the HLA-B27 can be achieved through a pair of primers and a probe. The Tm value in the melting curve instead of the Ct value of the amplification curve is adopted for result judgment, qualitative accuracy is achieved, and the result is visual and easy to interpret.

The invention discloses a method for determining the contents of ester solvents and additives in a sodium-ion battery electrolyte by GC-MS. Through preparation of a diluent, selection of a program method and control of conditions, the types and contents of the solvents and the additives in the sodium-ion battery electrolyte can be rapidly and accurately determined in one step. Therefore, the method can be widely applied to the field of component analysis of the sodium-ion battery electrolyte.

The invention provides a method for determining the content of phenolic organic matters in tar residue and phenolic wastewater, which comprises the following steps: optimizing an MRM (Magnetic Resonance Modeling) method based on a GC-QQQ optimizer to obtain an optimized MRM method; detecting mixed standard solutions of phenol, o-methylphenol and p-methylphenol with different concentration gradients by using an optimized MRM method to obtain a linear regression equation corresponding to the characteristic ion flow peak area and the concentration of each compound, and taking the linear regression equation as a quantitative standard curve equation of each compound; the method comprises the following steps: extracting organic matters in a liquid phase and a solid content of coal tar and phenolic wastewater raw materials into an organic phase, and circularly washing the raw materials for multiple times by using an extraction solvent to obtain a to-be-detected organic phase; detecting a to-be-detected organic phase by using an optimized MRM method, and comparing and matching the quantitative ion pair and the qualitative ion pair with compounds in the standard solution to obtain a characteristic peak of the to-be-detected organic phase; and obtaining the content of each compound in the sample to be detected according to the peak area of the characteristic peak of each compound and the quantitative standard curve equation.

The invention relates to the technical field of chemical analysis, in particular to a method for detecting free carotenoid in chili. The detection method comprises the following steps: detecting the capsicum extract by using ultra-high performance liquid chromatography-tandem mass spectrometry, and the liquid chromatography in the ultra-high performance liquid chromatography-tandem mass spectrometry comprises the following mobile phase components: a mobile phase A: methanol or acetonitrile, formic acid and ammonium formate, the volume percentage of formic acid in the mobile phase A is 0.05-0.2%, the volume percentage of ammonium formate in the mobile phase A is 0.05-0.2%, and the volume percentage of ammonium formate in the mobile phase A is 0.05-0.2%; the concentration of ammonium formate in the mobile phase A is 4.5-5.5 mM; and the mobile phase B is an MTBE (Methyl Tert-Butyl Ether) solution containing 0.005 to 0.02 g / 100mL of antioxidant. The detection method can accurately detect the free carotenoid in the pepper and perform quantitative analysis, and meanwhile, the detection method is simple and rapid to operate, high in sensitivity and good in repeatability.

This invention discloses a method for detecting and analyzing toxic gases based on flame photometry. The method includes: determining the types and quantities of constituent atoms of each toxic gas according to its molecular formula; determining the characteristic elements of each toxic gas, and further determining the typical characteristic values ​​of each toxic gas; each toxic gas uniquely corresponds to a set of typical characteristic values; the set of typical characteristic values ​​is calculated from the types and quantities of atoms corresponding to the characteristic elements; measuring the constituent atoms and content of the gas to be tested using flame photometry, calculating the measured characteristic values ​​of the gas to be tested, and obtaining the set of measured characteristic values ​​of the gas to be tested; comparing the obtained set of measured characteristic values ​​with known sets of typical characteristic values; if the set of measured characteristic values ​​completely corresponds to a certain set of typical characteristic values, then the gas to be tested can be determined to be the toxic gas corresponding to that set of typical characteristic values.

The invention provides a qualitative and quantitative analysis method for alkane organic matters in a complex matrix, which comprises the following steps: optimizing an MRM method based on a GC-QQQ optimizer, detecting a plurality of n-alkane mixed standard solutions with different concentration gradients, selecting a quantitative ion pair and a qualitative ion pair, establishing a linear regression equation based on the quantitative ion flow peak area and the concentration of each compound, and calculating the qualitative and quantitative analysis result of the alkane organic matters in the complex matrix. Taking the linear regression equation as a quantitative standard curvilinear equation of each compound, and counting the standard retention time of each compound; extracting organic matters in a liquid phase and a solid content of a to-be-detected sample into an organic phase, and circularly washing the raw material for multiple times by using an extraction solvent to obtain a to-be-detected organic phase; detecting a to-be-detected organic phase by using the optimized MRM method to obtain an ion flow peak area and retention time of the to-be-detected organic phase; and obtaining the content of each compound in the to-be-detected sample based on the ion flow peak area of the to-be-detected organic phase and the quantitative standard curve equation, and determining the nature of the compounds based on the retention time of the to-be-detected organic phase and the standard retention time.

The invention discloses an integrated micro-fluidic chip system for detection, photothermal decomposition and verification of perfluorinated and polyfluoroalkyl substances. The integrated micro-fluidic chip system comprises an upper ultraviolet light-emitting layer, a middle micro-fluidic main body layer and a lower constant-temperature heating layer, the middle microfluidic main body layer internally comprises an inlet channel, an optical detection module, a photothermal decomposition chamber, a circulating pump structure, a residue verification chamber and an outlet channel; the upper ultraviolet light-emitting layer and the lower constant-temperature heating layer are respectively arranged corresponding to the photothermal decomposition chamber so as to provide a light field and a thermal field which have a synergistic effect; the system further comprises an external control circuit for controlling the operation of the circulating pump structure, the upper ultraviolet light-emitting layer and the lower constant-temperature heating layer, and controlling the path selection of the fluid according to the output result of the residual verification chamber, so as to realize a closed-loop treatment flow. According to the three-layer micro-fluidic chip system provided by the invention, efficient and reliable treatment on perfluoro and polyfluoroalkyl substances is realized through photo-thermal synergistic degradation and closed-loop feedback control.

The application provides a non-target detection device and system based on a nanopore and a detection method thereof, the non-target detection device and system based on a nanopore are used for non-target detection of a complex matrix through a nanopore, feature extraction is performed on a nanopore detection graph, and sample classification is realized through model construction, the detection method does not make a prior definition on a to-be-detected target, and various molecular information existing in serum is acquired in a net catching manner, non-target capture and overall state analysis of various molecules in serum are realized, and a new way of rapid and portable disease screening is provided.The application breaks through the limitation of traditional single marker detection by combining the nanopore technology and machine learning, and is expected to promote the development of precision medicine in a more efficient and comprehensive direction.

The invention belongs to the field of analytical chemistry, and particularly relates to a method for determining 4-(4-amino-3-fluorophenoxy) pyridine-2-carboxylic acid methylamine and 2-chloro-5-aminobenzotrifluoride in a medicine, the determination method is a liquid chromatography, a C8 chromatographic column is adopted, a mobile phase A is selected from a phosphate solution, a mobile phase B is selected from a nitrile solvent, and gradient elution is carried out. The detection method has good specificity, repeatability and accuracy and high sensitivity, can effectively and accurately perform qualitative and quantitative analysis on the compound, and ensures safety and effectiveness of medicine quality.

The application discloses biomarkers for atherosclerosis diagnosis and application thereof. The biomarkers for atherosclerosis diagnosis include any one or any combination of PG(38:7), PG(34:2), PG(34:3), PG(38:6), PG(36:6), PG(38:5), PG(36:3) and PG(36:4). The biomarkers provided by the application have a strong correlation with advanced atherosclerotic plaques, and can be used for diagnosis and experimental research of atherosclerosis, especially advanced atherosclerotic plaques.

The invention discloses a detection method for ellagic acid and its metabolites, and relates to the field of detection and analysis technology. The method of the present invention optimizes the pre-treatment method and detection technology, and adopts a composite extractant N-methylpyrrolidone-methanol solution to ultrasonically extract substances rich in ellagic acid and urolithin, and the extracted samples are qualitatively and quantitatively determined by high performance liquid chromatography tandem ultra-high resolution mass spectrometer, and at the same time, according to the experimental results, a simple high performance liquid chromatography determination method is developed. This method breaks the shortcomings of the traditional detection method that ellagic acid and urolithin substances have poor solubility and are difficult to extract such substances in the sample, and the quantitative results are inaccurate. The method has simple processing steps, good separation effect, short detection time, high sensitivity, qualitative accuracy, simple operation, good specificity, and can analyze the changing trend of the ellagic acid metabolic pathway, providing new ideas and methods for studying its biological metabolic process.

The present invention provides a biosensor and a kit for detecting algae toxins, including an aptamer Apt, a T chain, a waste chain, a primer1 chain, a primer2 chain, an auxiliary chain H, a rolling circle template chain padlock and a hairpin HP, and the nucleotide sequence thereof is shown in SEQ ID NO: 1-8. The sensor of the present invention has good analytical performance, and has the advantages of being visible to the naked eye, fast detection speed, simple operation, low price, low detection limit, and high specificity. It can make up for the defects and shortcomings of the existing detection methods of microcystin, and realize rapid and accurate quantitative detection thereof. The kit prepared by this research has methodological versatility for the detection of microcystin, and has potential application prospects in food samples and environmental monitoring.

The invention discloses a detection method for oligomeric isomaltosaccharide content in a formula milk powder, including: S1: preparing hydrochloric acid, sodium hydroxide solution, PBS buffer and beta-galactosidase-glucose oxidase solution; S2: weighing the standard of each sugar respectively, configuring it into standard working solutions of different concentrations; S3: using high performance liquid chromatography, obtaining the peak area values ​​of standard working solutions of different concentrations, and using the peak area value as the ordinate, using the concentration value of the corresponding standard working solution as the abscissa, establishing a standard curve; S4: weighing the sample to be tested, adding beta-galactosidase-glucose oxidase solution to configure it into liquid to be tested; S5: obtaining the peak area value of liquid to be tested by high performance liquid chromatography, checking the concentration of each sugar in the liquid to be tested in the corresponding standard curve according to the peak area value, and then obtaining the content of each sugar in the sample to be tested by conversion formula. The present invention achieves the purpose of accurately detecting the content of oligomeric isomaltosaccharide in formula milk powder.

The invention belongs to the technical field of medicine detection, and particularly relates to a high performance liquid chromatography method for simultaneously detecting methyl p-toluenesulfonate and ethyl p-toluenesulfonate in an upatinib bulk drug. According to the high performance liquid chromatography method for simultaneously detecting methyl p-toluenesulfonate and ethyl p-toluenesulfonate in the upatinib bulk drug, high performance liquid chromatography is adopted for detection, and the chromatographic conditions are as follows: octadecylsilane chemically bonded silica serves as a filler of a chromatographic column, the detection wavelength is 220-230 nm, the column temperature is 33-37 DEG C, the detection time is 20-30 min, and the detection time is 30-50 min. And carrying out isocratic elution by taking an acetonitrile-phosphoric acid buffer solution system as a mobile phase. The invention provides a high performance liquid chromatography method for simultaneously detecting methyl p-toluenesulfonate and ethyl p-toluenesulfonate in the upatinib bulk drug, which is simple, convenient, rapid, high in specificity, high in sensitivity and good in separation degree, and improves the drug safety.

This invention provides a kit for detecting the pathogen of turtle mumps disease and its usage method, belonging to the field of biodetection technology. The kit includes a tissue pathogen release buffer, magnetic macroporous SiO2 microspheres, nucleic acid extraction solution, and detection reagents for the turtle mumps disease pathogen. This invention uses the tissue pathogen release buffer to release pathogens from tissues, magnetic macroporous SiO2 microspheres for pathogen enrichment, and nucleic acid extraction reagents to obtain a crude extract that can be directly used for LAMP amplification and qPCR amplification detection.

The invention discloses a method for simultaneously analyzing different types of liquid crystal monomers based on GC-APCI-MS / MS technology, which belongs to the technical field of analytical chemistry. Anhydrous sodium sulfate and recovery rate indicators PCB-28-d4 and PCB-114-d4 are added to a sample to be tested, a mixed solution of dichloromethane and n-hexane is added, the mixture is evenly mixed, ultrasonic extraction is performed, supernatant is obtained by centrifugation, the above steps are repeated, the extracts are combined, concentrated, and an internal standard is added. 13 C 12 ‑PCB‑141 was added to the fixed volume and detected by GC‑APCI‑MS / MS technology. The detection limit range of the method of the present invention for LCMs was 0.01‑0.2 ng / mL, the quantification limit range was 0.05‑1.2 ng / mL, and the repeatability of the quantitative lower limit for 6 consecutive injections was within 10% (0.26%‑7.01%).

The invention provides a method for simultaneously detecting five corticoids in a blood sample. The five corticoids are cortisol, cortisone, corticosterone, 21-deoxycortisol and 11-deoxycortisol respectively. The method comprises the following steps: pre-treating a blood sample by adopting the steps of precipitating protein, adding a specific buffer solution and the like, then detecting a target sample solution by adopting a high performance liquid chromatography-tandem mass spectrometry, establishing a standard curve by adopting an internal standard method, and calculating the contents of the five corticoids in the blood. The detection method has the advantages of being high in detection sensitivity, high in flux, simple, convenient, rapid, high in detection efficiency and the like, cortisol, cortisone, corticosterone, 21-deoxycortisol and 11-deoxycortisol in blood can be detected at the same time, accurate qualitative and quantitative analysis can be conducted, clinical application and scientific research cost is reduced, and high application value is achieved.

The invention discloses a method for qualitatively and quantitatively analyzing new psychoactive substances in tobacco tar through in-situ ionization-quadrupole rod time-of-flight mass spectrometry, an electronic tobacco tar sample does not need to be pretreated and can be directly diluted with an internal standard solution, then in-situ ionization-quadrupole rod time-of-flight high-resolution mass spectrometry analysis can be carried out, and only several minutes are needed. The method can obtain comprehensive information of a complex electronic cigarette liquid sample at m / z 50-1000Da to realize qualitative analysis, can make a standard curve through an internal standard method to carry out accurate quantification, and realizes qualitative and quantitative analysis at the same time in an ultra-fast manner. The rapid, accurate and high-sensitivity qualitative and quantitative analysis method is established by taking the new psychoactive substances in the electronic cigarette liquid as research objects, and technical support can be provided for rapid screening of the new psychoactive substances.

The invention discloses a method for detecting the content of crocin in a saffron crocus extract. The method comprises the following steps: drawing a standard curve of crocin-I and crocin-II; performing ultra-high performance liquid chromatography detection on the saffron crocus extract; the used mobile phase comprises a mobile phase A and a mobile phase B; the mobile phase A is water, and the mobile phase B is acetonitrile; a gradient elution procedure is adopted for elution, the volume fraction of the mobile phase A is 80%-60% in 0-4 min, the volume fraction of the mobile phase B is 20%-40%, the volume fraction of the mobile phase A is 60%-80% in 4-4.01 min, the volume fraction of the mobile phase B is 40%-20% in 4-4.01 min, the volume fraction of the mobile phase A is 80%, and the volume fraction of the mobile phase B is 20% in 4.01-5 min; and substituting the obtained peak area into a standard curve, and calculating the concentration. According to the method, an ultra-performance liquid chromatograph is selected as a detection means for optimizing extraction conditions, the detection quantity is low, the sensitivity is high, the repeatability is good, the stability is high, and accurate qualitation and quantification can be achieved.