Method for synchronously detecting six sweetening agents in distilled spirit by using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer

A triple quadrupole, ultra-high performance liquid phase technology, applied in the field of detection of sweeteners in liquor, can solve the problems of difficult inspection and few detection methods of sweeteners, and achieve simple and fast operation, excellent separation effect, and repeatability Good results

Active Publication Date: 2015-05-20
ANHUI GUJING DISTILLERY +1
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  • Abstract
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Problems solved by technology

However, due to the lack of detection methods suitable for sweeteners in liquor, it has caused difficulties for relevant inspection departments

Method used

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  • Method for synchronously detecting six sweetening agents in distilled spirit by using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer
  • Method for synchronously detecting six sweetening agents in distilled spirit by using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer
  • Method for synchronously detecting six sweetening agents in distilled spirit by using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer

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Embodiment 1

[0058]The method for simultaneously detecting 6 kinds of sweeteners in liquor by an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer of the present invention uses an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer UPLC-MS / MS, including Ultra-high performance liquid chromatography UPLC and triple quadrupole tandem mass spectrometer MS / MS; the method is to proceed as follows:

[0059] (1) Pretreatment of liquor to be tested

[0060] Pipette 5mL of a liquor to be tested into a colorimetric tube, and process it with a nitrogen blower to remove the alcohols in the liquor to be tested until 1-2mL of the liquor to be tested remains, then dilute to 5mL with ultrapure water, and pass through 0.22 The sample to be tested is obtained after filtration with a μm water filter membrane;

[0061] (2) Condition setting of the instrument used

[0062] The condition of setting described ultra-high performance liquid...

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Abstract

The invention discloses a method for synchronously detecting six sweetening agents (namely acesulfame, saccharin sodium, sodium cyclamate, aspartame, trichlorosucrose and neotame) in a distilled spirit by using an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer. The method comprises the following steps: performing nitrogen blowing on a wine sample to remove alcohol, separating the wine sample by using an ultra-high performance liquid chromatograph, and then performing multi-reaction ion monitoring mode detection by using a triple quadrupole tandem mass spectrometer. The six sweetening agents have good linear relationships in a range of 50-1000ng/mL, correlation coefficients r2 of the six sweetening agents are more than 0.993, the average recovery rate of the sample is 90-120%, and the limit of detection is 2.69-5.16ng/mL. The method disclosed by the invention is accurate, reliable, simple and fast, and can be used for synchronously detecting the six sweetening agents in the distilled spirit.

Description

technical field [0001] The invention relates to a method for detecting sweeteners in liquor, in particular to an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometer for simultaneously detecting six kinds of sweeteners in liquor, which belongs to the wine analysis technology field. Background technique [0002] Baijiu is a traditional Chinese product with a history of thousands of years, and its mellow and sweet taste is deeply loved by consumers. However, many wine companies add sweeteners to improve the taste for their own interests, and some companies use wine flavors and fragrances containing sweeteners due to lax purchase of raw materials, resulting in sweetness in liquor products. Dosage exceeded. Frequent reports of this phenomenon in the media have caused panic among consumers in the market. Studies have shown that the intake of high doses of sweeteners can cause damage to the human liver and nervous system, and even cause cancer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 刘国英汤有宏王银辉李亭亭李安军马雷
Owner ANHUI GUJING DISTILLERY
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