234 results about "Mass spectrometry detector" patented technology

The invention discloses a measurement method of a volatile organic compound in tobacco lining paper with high determination accuracy and good repeatability, which is suitable for simultaneously determining dozens of volatile organic compounds. The measurement method is as follows: taking fluorobenzene as an internal standard substance; using headspace gas chromatography for measuring the content of the volatile organic compound in the tobacco lining paper; wherein a detector in the headspace gas chromatography is a mass spectrometry detector. The measurement method takes the fluorobenzene as the internal standard substance, uses the headspace gas chromatography/mass spectrometry for measuring the content of the volatile organic compound in the tobacco lining paper, and has the following advantages that the fluorobenzene has stable chemical property and are very similar to determinand in every performance, and the peak time appears in the middle of 19 target VOCs to be detected; therefore, the fluorobenzene is the very suitable internal standard substance, can eliminate the influence of ground substance in the tobacco lining paper on the measurement, causes materials which can not or are difficult to peak to be measured, and has high measurement accuracy and few interference factors.

The invention discloses a chiral analysis method for nicotine in cigarette cut tobacco. The chiral analysis method for nicotine in cigarette cut tobacco is characterized by including the steps that cut tobacco samples are dried, smashed and screened, extracting liquid is added, the mixture is extracted and filtered, then common cut-tobacco-sample extracting liquid is directly analyzed through a multidimensional gas phase chromatography and mass spectrometry detector, cut-tobacco-sample extracting liquid with the excessively-low nicotine content is subjected to automatic solid phase microextraction to be subjected to multidimensional gas-phase-chromatography tandem-mass-spectrum analysis, and the proportion of S-(-)-nicotine and R-(+)-nicotine in the cigarette cut tobacco is quantitatively detected with the peak area normalization method. The detection method is simple, fast and convenient in sample treatment, low in method detection limit, high in sensitivity and good in accuracy degree, and analysis of cigarette cut tobacco samples can be met.

The invention provides a method for testing a spectrum of amino acid and organic acid in urine, wherein, a conventional protein deposition treatment is carried out on biological samples; capillary electrophoresis is adopted as a separate method; and a mass spectrometry detector which has good universality and high sensitivity is adopted as a detector. The method has the advantages of high precision and recovery, derivatization omission, simple operation, high sensitivity and good selectivity and universality and can simultaneously analyze 22 amino acids and 5 organic acids in the biological samples.

The invention provides a system and a method for measuring the content of 57 volatile organic compounds in ambient air. The system comprises a four-way valve, a gas chromatograph and a three-stage cold trap preconcentrator; the four ports of the four-way valve are respectively connected with a pre-separation column, a first secondary separation column, a second secondary separation column and a helium gas source; and the gas chromatograph is provided with a flame ionization detector and a mass spectrometry detector. The method comprises the following steps: (1) preparing a mixed standard use gas; (2) preparing an internal standard use gas; (3) collecting an ambient air sample; (4) establishing a standard curve of a target component; and (5) taking the ambient air sample to be tested, removing interfering substances through the three-stage cold trap preconcentrator, performing gas chromatography-mass spectrometry analysis in the mass spectrometry detector after focusing is performed, obtaining the content of each component according to a peak area and the standard curve established in the step (4), and calculating the concentration of the sample to be tested according to a sample injection volume of the sample to be tested. By adopting the method provided by the invention, the simultaneous analysis of two detectors is achieved by once sample injection, thereby avoiding the influence of the interfering substances on the measurement result simply and effectively.

The invention belongs to the field of analytical chemistry, and specifically relates to a method for determining short-chain chlorinated paraffins in plastics, rubber, and textile materials. (2) purification by sulfonation with concentrated sulfuric acid; and (3) detection step (2) using gas chromatography-electron capture detector or gas chromatography-negative chemical ionization (NCI) mass spectrometry detector The content of short-chain chlorinated paraffins in the obtained purified samples. Beneficial effects of the present invention: Utilize concentrated sulfuric acid sulfonation purification method, can effectively remove interference, use gas chromatography-electron capture detector and NCI mass spectrometry detector to detect medium and short-chain chlorinated paraffins in plastics, rubber, textile materials, can realize Accurate quantification; according to the specific mass spectrum of short-chain chlorinated paraffins, the interference of medium and long-chain chlorinated paraffins can be effectively eliminated, making the qualitative more accurate, the selectivity stronger, and the sensitivity higher.

The invention discloses a biological sample spectral analysis method based on a solute migration electrospray ionization technique. The biological sample spectral analysis method comprises the following steps: 1, under the conditions of normal temperature and normal pressure, firstly, injecting a solvent into a nanolitre spraying capillary tube; 2, loading a sample at the tail end of a solvent or in the middle of the solvent, applying direct-current voltage to the nanolitre spraying capillary tube so as to dissolve a target compound in the sample into the loaded solvent and migrate to the top end of the nanolitre spraying capillary tube, and furthermore performing electric spraying and detecting corresponding signals by using a mass spectrometry detector, thereby obtaining analysis result. The biological sample spectral analysis method has the characteristics that the operation is simple and convenient, no sample pretreatment is needed, the amount of the sample is small, the analysis sensitivity is high, real-time rapid analysis on the target compound is achieved, and the like.

The invention provides an ultrahigh pressure liquid phase detection method for a Chinese patent medicine containing at least two medicinal materials of white paeony root, ginseng, root of red-rooted salvia, sweet worm wood herb, liquoric root, szechuan lovage rhizome, Chinese angelica, astragalus and nutgrass galingale rhizome. The method comprises the following steps of: preparing solution of a test sample and solution of a contrast medicinal material; performing ultra performance liquid chromatography (UPLC) detection by taking acetonitrile-water as a mobile phase and using an ultraviolet detector and/or a mass spectrum detector, wherein the detection wavelength is between 201 and 320nm; and performing result detection, namely detecting whether a corresponding chromatographic peak exists or not at a position where the retention time is as same as that of a contrast medicinal material chromatogram in a chromatogram of the test sample. Compared with thin layer identification and high performance liquid chromatography (HPLC) detection methods, the ultrahigh pressure liquid phase detection method successfully improves the resolution of the chromatogram, shortens the analysis cycle, and ensures more precise and stable results.

The invention belongs to the technical field of gas or volatile sample separation and analysis and particularly provides a portable gas chromatography-mass spectrometry linkage device. The device is divided into a gas chromatography module, an ionization source module and a mass spectrometry detector module; the gas chromatography module comprises a gasification chamber, a column oven and a gas chromatographic column; the ionization source module comprises an ion source and an ion lens; the detector module comprises an ion transmission device and a mass analyzer. The centers of the three modules are located on the same axis, the gas chromatography module is connected with the ion source, and the ionization source module is connected with the ion transmission device of the mass spectrometry detector module through the ion lens. According to the portable gas chromatography-mass spectrometry linkage device, mass spectrometry negative pressure is utilized, air serves as carrier gas, the device is simplified, the weight is reduced, and it is more convenient to achieve vehicle mounting or miniaturization for site survey; sample feeding and carrier gas switching processes are achieved through a three-way valve, the structure is simple, and it is convenient to feed samples quantitatively; the device can be effectively used for real-time detection and analysis of gas samples or volatile samples, and industrial utilization value is achieved.

The invention provides a quality control method for shenmai injection mass spectrum finger prints. Liquid chromatogram is adopted as a separation measure, and a mass spectrum detector is taken as a detection measure so as to obtain two finger prints for representing compound information of red ginseng and radix ophiopogonis in shenmai injection; and the similarity is calculated by adopting a correlation coefficient method, and samples with the similarity more than or equal to 0.9 are judged as products with stable quality. In the quality control method, the characteristics that the mass spectrum detector has high sensitivity and strong specificity, and can sample in multiple channels are utilized, and a method for detecting in the multiple channels and with different modes is selected, sothat the two finger prints can be obtained by one-time analysis, the compound information of red ginseng and radix ophiopogonis in a preparation can be reflected simultaneously, the completeness and the specificity of the information of the finger prints of the preparation are ensured, the repeatability is good, and the technical guarantee is provided for the quality control of the shenmai injection.

The invention relates to the technical field of marine sediment contamination detection, in particular to a dispersive solid-phase extraction-gas chromatography-mass spectrometry detection method of aphthalic acid ester environmental hormone in a marine sediment. The method comprises the following steps of: (1) sample collection, (2) sampling and ultrasonic extraction, (3) concentration, (4) dispersive solid-phase extraction purification, (5) concentration and constant volume, (6) detection by a gas chromatography-mass spectrometry detector, (7) standard curve drawing and (8) determination ofa recovery rate. The method is simple to operate; the ultrasonic extraction efficiency is high; pretreatment of a sample can be accomplished quickly; graphitized carbon black is used as a solid-phaseadsorbent for the first time; the dispersive solid-phase extraction efficiency is high; a purification effect is good; the method is higher in sensitivity, recovery rate and repeatability; a detection limit of 16 phthalic acid esters is 0.1-0.25 micrograms per kg; the recovery rate is 78-117%; a relative standard deviation (n=5) is 2.4-6.8%; and the method can be applied to the accurate determination of a phthalic acid ester content in the marine sediment.

The invention discloses a method for measuring phenolic estrogen by means of derivation. The method includes simultaneously deriving six types of phenolic estrogen; quickly measuring the phenolic estrogen by the aid of ultrahigh-performance liquid chromatography-mass spectrometry detectors. Compared with the prior art, the method has the advantages that the sensitivity of the six types of phenolic estrogen can be improved, the method is good in repeatability and low in detection cost and only has small variable coefficients, and measurement requirements on recovery rates can be met.

The invention discloses a method for measuring the content of gingerol in ginger medicinal materials and preparations thereof. By virtue of the separation and analysis technology of ultra-high performance liquid chromatography in series with a triple quadrupole mass spectrometer detector and an ultraviolet detector as well as a one-measurement multi-evaluation method, a pure substance N-vanillyl nonane amide reference substance which does not exist in a sample, has stable properties and is easy to obtain is adopted as an internal reference substance so as to establish a relative correction factor between the component and four gingerol components in the sample, thereby realizing measurement of the contents of the four gingerol components in the ginger medicinal materials and the preparations thereof by virtue of calculation of the correction factor. The method disclosed by the invention is simple in operation, high in sensitivity, accurate and efficient and low in cost, can be used forobjectively and accurately evaluating the quality of the ginger medicinal materials and the preparations thereof, can be used for quality control, can solve the problem that the quality of the medicinal materials and the preparations thereof can not be objectively and reasonably controlled due to the lack of reference substances, and has important significance for controlling quality and ensuringcurative effects.

The invention discloses an identification method and an application of an insulin mass spectrum peptide graph. According to the method, a V8 enzyme is adopted, and the V8 enzyme is combined with lysylendopeptidase, specific hydrolysis of insulin is carried out, and a peptide graph containing insulin characteristic fragments is established through a liquid chromatograph-mass spectrometer. The method is characterized in that a buffer solution is used for providing an enzymolysis environment with the pH value of 7.5-9.5, wherein a substrate and the enzyme are reacted overnight according to the mass ratio of insulin to V8 enzyme to lysyl endopeptidase of (100-1000) to (100-500) to 1, the reaction liquid is diluted for 10-30 times, a liquid phase separation mass spectrometry detector is adopted for detection, and a characteristic peptide graphs of a variety of insulin are established. By adopting the mass spectrum peptide graph, various different kinds of insulin can be accurately determined, and the method is accurate and reliable.

The invention discloses a method for synchronously measuring blood drug concentrations of multiple antidepressants, wherein the method comprises the steps of: pre-processing a sample to be measured, analyzing the chromatographic column separation, and performing the detection by a mass detector. The method has the advantages that the samples are a few, the pre-processing procedure is simple, the analysis period is short, the detection sensitivity is high, the blood drug concentrations of a plurality of antidepressants can be detected synchronously, the application is wide, the cost is low, and the method is suitable for monitoring conventional blood drug concentrations.

The invention relates to a method of determining a residual quantity of methylmercury in aquatic products. The method comprises mixedly extracting samples by a given volume of hydrochloric acid of a certain concentration and a sodium chloride solution, extracting with toluene, stripping with an L-cysteine ??solution, deriving with a given volume of derivative agents of two concentrations, pre-treating with n-hexane extraction, measuring the methylmercury by using a gas chromatography-mass spectrometry, wherein a temperature of an introduction port of the gas chromatography is set to be 250-300 DEG C, a carrying gas is set as a constant voltage mode, a nonpolar chromatographic column is selected and an interface temperature is 260-300 DEG C, passing the pre-treated sample through the gas chromatography and then into the chromatographic column, then heating, separating the sample in the chromatographic column, then entering into the mass spectrometry, determining nature of the sample according to a retention time of object compounds, characteristic ions and an abundance ratio, and determining an amount of the sample by an external-standard peak-area method. The method provided by the invention has advantages of accurate analysis method, good selectivity, high sensitivity and small interference.

The invention provides a method for detecting ethyl p-toluenesulfonate as genetic toxicity impurities in ibuprofen by gas chromatography-mass spectrometry. The method comprises the following main steps: taking 5% of phenyl-arylidene and 95% of dimethyl polysiloxane as a fixed-phase capillary column; preparing a reference substance linear solution and a sample solution; carrying out temperature programming; after samples are separated by a gas chromatograph, detecting the samples by a mass spectrometry detector, wherein a mass spectrum is an EI source; quantifying selected ions in a scanning mode, wherein the selected ions are m/z: 91, 92, 155; calculating an equation of linear regression by the concentration of a reference substance and the corresponding peak area; and calculating the content of the ethyl p-toluenesulfonate in the ibuprofen by the equation of linear regression. The method is simple, speedy and accurate, and is high in sensitivity; the detection method is scientific andobjective; and the method can be used for controlling the quality of an ibuprofen sample, and has practical value.

The invention relates to a <18>O on-line marked protein quantitative analysis platform, and an operation method thereof. The <18>O on-line marked protein quantitative analysis platform is a system integrating protein on-line enzymolysis, <18>O on-line marking and polypeptide separation and detection. The <18>O on-line marked protein quantitative analysis platform comprises an injection pump, a sampling needle, a column oven, an enzyme reactor, a switch valve, a peptide fragment capture column, a liquid phase chromatography pump, a polypeptide separation column and a mass spectrometry detector. A protein sample is firstly loaded to the enzyme reactor through the injection pump to carry out incubation; <18>O marking can be carried out at the same time with enzymolysis; and the <18>O marked peptide fragment after enzymolysis is enriched on-line and transferred into the polypeptide separation column for further separation, and finally is detected by the mass spectrometry detector. The <18>O on-line marked protein quantitative analysis platform integrates the protein on-line enzymolysis, <18>O on-line marking and polypeptide separation.

The invention belongs to the technical field of sample separation and analysis of isomeride, and specifically to a three-dimensional ion mobility combinational mass spectrometer. The three-dimensional ion mobility mass spectrometer comprises an ionization source module, an ion mobility module and a mass spectrometry detector module, wherein the ionization source module comprises an ion source spraying needle and a desolvation region; the ion mobility module comprises a first ion focusing lens, an ion trap, an inlet end baffle, a buffering gas outlet, annular electrodes, heating rings, strong asymmetric electrodes, a buffering gas inlet and a second ion focusing lens; the mass spectrometry detector module comprises an ion transmission four-stage rod and a flight time mass spectrum; the centers of the ionization source module, the ion mobility module and the mass spectrometry detector module are on the same axle wire; the ionization source module is connected to the first ion focusing lens of the ion mobility module; and the ion mobility module is connected with an ion transmission focusing apparatus of the mass spectrometry detector module through the second ion focusing lens. According to the three-dimensional ion mobility combinational mass spectrometer, the annular overlapped electrodes are adopted to drive ions to fly, and the buffering gas enters from the negative direction; and meanwhile, a one-dimensional electric field is further added to the third dimension, so that the collision probability between the buffering gas and ions is increased, and the separation efficiency is greatly increased.

The invention relates to a high performance liquid chromatography-time-of-flight mass spectrometry method for identifying a degradation product in a compound cold treatment medicine based on a heart-cutting technology. The method comprises the following steps: separating a sample by using a mass spectrum incompatible mobile phase in a first-dimensional chromatograph; acquiring ultraviolet data through a one-dimensional detector; determining a target peak, and carrying out heart-cutting to make the target peak exist in a sample loop; carrying out back flushing through valve switching by using a two-dimensional system using a mass spectrum compatible mobile phase to make the target peak exist in the two-dimensional system and enter a two-dimensional ultraviolet and mass spectrum detector in order to complete impurity analysis and identification. The method has the advantages of no change of original separation conditions, high specificity, accuracy and resolution in impurity detection, and improvement of the reliability of nonvolatile buffer salt separation system impurity mass spectrometry and identification.

The invention belongs to the field of analytical chemistry, and in particular relates to a method for determining the content of tris (2-chloroethyl) phosphate, which is characterized in that it comprises the following steps in sequence: (1) extracting with an organic solvent; (2) extracting from step (1) The obtained extraction solution is filtered; (3) the filtered extraction solution is determined by a high performance liquid chromatography-mass spectrometer detector. The invention has the beneficial effects of detecting tris(2-chloroethyl)phosphate with a high-performance liquid chromatography-mass spectrometer, strong anti-interference ability, convenient and fast sample pretreatment, accurate qualitative and quantitative, and avoiding the target The decomposition of compounds has stronger selectivity and higher sensitivity.

The invention provides a system and a method for determining phthalate in atmospheric particulates, and relates to environment-friendly monitoring method and equipment. The problem that the pollution is easily introduced in the laboratory analysis test process of phthalate is solved. The system comprises a to-be-determined sample adhered with atmospheric particulates, a desorption tube, a heating device, an inert gas flow mechanism, a collecting trap mechanism, a liquid nitrogen cooling mechanism, a chromatographic column and a mass spectrometry detector. The method comprises the following steps: heating the desorption tube filled with the to-be-determined sample; brining to-be-determined component released from the to-be-determined sample into the collecting trap mechanism to cool and enrich by using the inert gas flow; heating the collecting trap mechanism, and brining the desorbed to-be-determined component into the chromatographic column by using the inert gas flow; performing chromatographic separation on the desorbed to-be-determined component by using the chromatographic column; and detecting the phthalate in the atmospheric particulates according to the chromatographic separation result obtained by the chromatographic column by using the mass spectrometry detector.

The invention discloses a method for determining the migration quantity of volatile organic compounds in an adhesive sticker for a food label in a water-based food simulating object. The method comprises the following steps: performing a direct or indirect migration test on the adhesive sticker for the food label through a water-based food simulating solution; sampling the water-based food simulating solution through purging and trapping; separating 10 volatile organic compounds through HP-5ms or chromatographic columns with similar properties; performing detection through a mass spectrometry detector; performing quantification through an external standard method, wherein the 10 volatile organic compounds include nonene, 2-ethylhexanol, 1,2,4,5-tetramethylbenzene, camphor, 1-dodecene, naphthalene, 2-ethylhexyl acrylate, 1-methylnaphthalene, 1-undecanol and dodecyl aldehyde. The method is accurate in test, easy to operate, high in efficiency, quick, high in sensitivity and high in practicality, is applicable to qualitative and quantitative determination on the volatile organic compounds in the adhesive sticker for the food label through a manufacturing enterprise of the adhesive sticker for the food label and relevant government supervision departments, and can be used for avoiding harm to the physical health of a consumer caused by over-large migration quantity of the volatile organic compounds.

A pulse fusion mass spectrometry element analysis method belongs to the technical field of quantitative analysis of chemical components of materials. The steps are: pulse melting and degassing: under the protection of inert gas, the pulse furnace is used to heat and melt the sample, and the oxygen, nitrogen, hydrogen and argon gas elements in inorganic materials such as metals and ceramics are converted into gaseous components in a graphite crucible. Carbon monoxide and carbon dioxide, nitrogen, hydrogen and argon are released; purification treatment: the degassed product is carried out by the carrier gas and purified; mass spectrometry detection: the purified gas is introduced into the mass spectrometer, and the degassed product is ionized and focused into an ion beam , is accelerated by an electric field, separated according to the mass-to-charge ratio, and finally reaches the mass spectrometer to generate a series of electrical signals; after signal collection and data processing, the mass percentages of oxygen, nitrogen, hydrogen and argon elements in the tested sample are obtained. The advantage is that it can realize the simultaneous, fast, high sensitivity and high precision analysis of the above four elements. Sensitivity of 0.1ppm to 0.01ppm.