53 results about "Catecholamine" patented technology

The present application is applicable to the field of medical devices, and discloses a liquid chromatography-mass spectrometry apparatus and a control method for the liquid chromatography-mass spectrometry apparatus. The liquid chromatography-mass spectrometry apparatus comprises a sample storage device, a pretreatment device, a chromatography device, a mass spectrometry detection device, and a controller. The chromatography device comprises a test solution preparation channel, a test solution transfer assembly, a liquid phase fluid delivery assembly, and a chromatography column. An operating pressure outputted by the liquid phase fluid delivery assembly is less than or equal to 20 MPa. The length of the chromatography column is greater than or equal to 3 mm and less than or equal to 35 mm. A sample comprises a target substance to be measured, and the target substance to be measured comprises at least one of vitamin D, a steroid hormone, and a catecholamine. The present application reduces costs of the liquid chromatography-mass spectrometry apparatus.

The present invention provides compounds of formula (I) that are prodrugs of catecholamine for use in treatment of neurodegenerative diseases and disorders. The present invention also provides pharmaceutical compositions comprising compounds of the invention and methods of treating neurodegenerative or neuropsychiatric diseases and disorders using the compounds of the invention, in particular Parkinson's disease.Accordingly, the present invention relates to compounds of formula (Id)whereinR1 is H and R2 is selected from one of the substituents (i) and (ii) below; orR1 is selected from one of the substituents (i) and (ii) below and R2 is H; orR1 and R2 are both represented by substituent (i) below; orR1 and R2 are both represented by substituent (ii) below; orR1 is substituent (i) and R2 is substituent (ii); orR1 is substituent (ii) and R2 is substituent (i);wherein * indicates the attachment point; andwherein the carbon atom at the attachment point on substituent (i) is in the S-configuration;or a pharmaceutically acceptable salt thereof.

The present invention provides sulfamate derivative prodrugs of the dopamine agonist (4aR,10aR)-1-propyl-1,2,3,4,4a,5,10,10a-octahydro-benzo[g]quinoline-6,7-diol, their use in treating conditions for which treatment with a dopamine agonist has therapeutic benefit, and pharmaceutical compositions comprising a compound of the present invention. The compounds according to the present invention have the formula (Id), wherein R1and R2are each independently selected from H and the substituent (iii) below, wherein * indicates the point of attachment to oxygen, wherein R3is selected from H and COR4, and wherein R4is selected from H and C1-C6alkyl, with the proviso that R1and R2cannot both be H, 15or a pharmaceutically acceptable salt thereof.

The present invention relates to a vanillic acid derivative compound which can be formed by a dehydration condensation reaction between vanillic acid and catecholamine. The vanillic acid derivative compound according to the present invention is characterized in that it can be used as a therapeutic preparation for Alzheimer's disease and neuroinflammation. In addition, the present invention relates to a therapeutic composition for Alzheimer's disease and a therapeutic composition for neuroinflammation, each of which comprises the compound.

Provided herein are methods and materials for preventing cytokine release syndrome (CRS). For example, methods and materials for using one or more catecholamine inhibitors to prevent a mammal from developing CRS are provided.

The purpose of the present disclosure is to provide a novel plant environmental stress alleviator, a plant growth promoter, and a plant DNA repair promoter that have an excellent effect of alleviating environmental stresses such as high temperatures.　The present disclosure provides a plant environmental stress alleviator, a plant growth promoter, and a plant DNA repair promoter containing: one or more neurotransmitters selected from choline esters, catecholamines, serotonin, and amino acids, and / or one or more acetylcholinesterase inhibitors selected from polyphenols, flavonoids, and organic acids, or a plant extract containing said inhibitors; and calcium ions. The present disclosure also provides a method for producing the aforementioned agents.

The invention discloses prevention and treatment of diseases by stimulating TRPV1 + sensory nerves. Specifically, the invention discloses application of a substance for stimulating TRPV1 + sensory nerves in preparation of products for prevention, treatment or adjuvant treatment of inflammatory diseases, autonomic nerve dysfunction diseases or adrenal insufficiency diseases, and application of the substance for stimulating TRPV1 + sensory nerves in preparation of products for inhibiting proinflammatory cytokines and products for promoting anti-inflammatory cytokines. The TRPV1 ion channel agonist disclosed by the invention can obviously and effectively inhibit the expression of numerous proinflammatory cytokines and obviously improve the expression of anti-inflammatory cytokines IL10, so that not only is the physiological status of an inflammatory mouse obviously improved, but also the survival rate of the inflammatory mouse is obviously improved; meanwhile, the spleen function can be adjusted, expression of spleen pro-inflammatory genes is remarkably inhibited, generation of catecholamine is increased, and secretion of cortisol hormones is promoted. The invention has wide clinical application value in prevention and treatment of related diseases.

The application discloses a method and a kit for detecting catecholamine and its metabolites in urine, and comprises the following steps: adding an internal standard working solution to a urine sample to be detected, mixing, then adding butyric anhydride solution, mixing, and then oscillating and mixing at 20-35 DEG C for 5-30 min to perform a derivatization reaction; after the derivatization reaction is completed, centrifugation is performed, the supernatant is taken, and liquid chromatography tandem mass spectrometry is used for detection. The toxicity and danger of butyric anhydride are low, the by-product after derivatization is only butyric acid, and the by-product does not interfere with catecholamine substance detection, and a neutral or weakly alkaline environment with a pH of 7.0-8.0 can meet the derivatization conditions. Since the pretreatment time is short, and the structure is changed after derivatization, the stability of catecholamine prototype substances is improved, meanwhile, the structure after derivatization is more easily ionized in a mass spectrometry ion source, the substance response intensity is also increased, and the detection result is more reliable.

The invention relates to the technical field of catecholamine detection, in particular to a copper-doped carbon dot with similar catechol oxidase activity as well as a preparation method and application of the copper-doped carbon dot. The copper-doped carbon dot provided by the invention can be used as a nano-enzyme for specific catalytic oxidation of catecholamine, so that catecholamine is detected by a colorimetric method. Copper atoms in the copper-doped carbon dots are dispersed in a monatomic form, and a local coordination structure is Cu-N3O which is similar to metal active sites in natural enzyme; according to the structure, the electron transfer capability and the redox activity of a Cu center are remarkably enhanced, so that the structure shows efficient catalytic activity and substrate specificity in a catecholamine oxidation reaction.

The invention relates to the technical field of analysis and detection, in particular to a kit and a method for extracting catecholamine and metabolites thereof based on a paramagnetic particle method and a method for detecting catecholamine and metabolites thereof by LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry). The kit comprises magnetic beads, an activating solution, an eluent, a washing solution, a leacheate I, a leacheate II and a derivatization reagent, the catecholamine and the metabolite thereof comprise one or more of dopamine hydrochloride, epinephrine, noradrenaline hydrochloride, methoxynoradrenaline hydrochloride, methoxyepinephrine hydrochloride and 3-methoxytyramine hydrochloride. The in-vivo concentration of catecholamine and metabolites thereof in human plasma and urine is detected in a high-flux manner by adopting a magnetic bead and derivation method, and the kit has the characteristics of high sensitivity, good repeatability, high accuracy, good specificity and the like.

The present invention provides compounds of formula (I) that are prodrugs of catecholamine for use in treatment of neurodegenerative diseases and disorders. The present invention also provides pharmaceutical compositions comprising compounds of the invention and methods of treating neurodegenerative or neuropsychiatric diseases and disorders using the compounds of the invention, in particular Parkinson's disease. Accordingly, the present invention relates to compounds of formula (Id) wherein R1 is H and R2 is selected from one of the substituents (i) and (ii) below; or R1 is selected from one of the substituents (i) and (ii) below and R2 is H; or R1 and R2 are both represented by substituent (i) below; or R1 and R2 are both represented by substituent (ii) below; or R1 is substituent (i) and R2 is substituent (ii); or R1 is substituent (ii) and R2 is substituent (i); wherein * indicates the attachment point; and wherein the carbon atom at the attachment point on substituent (i) is in the S-configuration; or a pharmaceutically acceptable salt thereof.

The invention provides a magnetic bead extraction unit, a magnetic bead extraction system and application of the magnetic bead extraction unit in catecholamine pre-packaging extraction, and designs the magnetic bead extraction unit which is pre-filled with a magnetic bead treatment reagent of a single sample and can be used for completing magnetic bead extraction before mass spectrometry of the single sample; according to the number of samples needing to be detected, a corresponding number of magnetic bead extraction units are selected and placed on the test tube rack, so that a magnetic bead extraction instrument can be matched to complete magnetic bead extraction of the required number of samples; a pre-packaging kit for extracting catecholamine in a sample is designed, and a magnetic bead activating agent, a washing solution and an eluent which are not easy to volatilize and higher in stability are screened. The magnetic bead extraction unit is simple in structure and convenient to use, the problem of waste of magnetic bead treatment reagents is avoided, the magnetic bead treatment process is more flexible and efficient, the pre-subpackaged reagents are stable in performance, not prone to volatilization and long in quality guarantee period, and the magnetic bead extraction unit is suitable for large-scale application and popularization.

Provided is stimulation of TRPV1+ and / or TRPA1+ sensory nerves for the prevention and treatment of diseases. Particularly provided is use of a substance for stimulating TRPV1+ and / or TRPA1+ sensory nerves in the prevention or treatment of inflammatory diseases, dysautonomia, or adrenal insufficiency, and in inhibiting the generation of pro-inflammatory cytokines and promoting the generation of anti-inflammatory cytokines. A TRPV1 and / or TRPA1 ion channel agonist can significantly effectively inhibit the expression of numerous pro-inflammatory cytokines and significantly improve the expression of the anti-inflammatory cytokine IL10, significantly improving both the physiological state and the survival rate of mice with inflammation. Moreover, the agonist can adjust spleen function, significantly inhibit the expression of pro-inflammatory genes in the spleen, increase the generation of catecholamine, and promote the secretion of cortisol-like hormones. The agonist has wide clinical application value for the prevention and treatment of related diseases.

Dirofilaria immitis (heartworm), is a filarial worm causing dirofilariasis in dogs. We have identified a gene encoding an S-adenosyl-Methionine dependent methyltransferase protein in heartworm, we named DiMT, that has orthologs in other filarial worms. RNAi silencing assays of a gene orthologous to DiMT in Caenorhabditis elegans have shown that knockdown of the orthologous gene is lethal to the worms. We discovered compounds that have an inhibitory effect against the catalytic activity of DiMT protein also consistently have lethal antiparasitic efficacy against the D. immitis microfilariae. Consequently, inhibiting enzymatic activity of DiMT led to the accumulation of catecholamines in the microfilariae which then led to paralysis and death of the parasites.

The present invention relates to a compound of formula (I) as inhibitors of calcium calmodulin dependent protein kinase 2 (CAMK2) for the treatment of for the treatment of heart failure, fibrosis, cardiomyopathies, atrial fibrillation, catecholaminergic polymeric ventricular tachycardia, heart block, cardiac arrhythmias, contraception, anxiety, post-traumatic stress disorder, hypertension, tachycardia, diabetes, allergy, and asthma.

The present invention provides compounds of formula (Id) that are prodrugs of catecholamine for use in treatment of neurodegenerative diseases and disorders. The present invention also provides pharmaceutical compositions comprising compounds of the invention and methods of treating neurodegenerative or neuropsychiatric diseases and disorders using the compounds of the invention, in particular Parkinson's disease.

The present invention provides a sample pretreatment method and kit for extracting 8 catecholamines and their metabolites from urine. The sample pretreatment method adopts a magnetic bead extraction method in combination with a fully automatic extractor for automatic extraction. The magnetic bead working solution in the magnetic bead extraction method is a suspension of magnetic beads, water and isopropanol, and the magnetic beads are a mixture of carboxyl magnetic beads and amino magnetic beads. The sample pretreatment method comprises: S100, the fully automatic extractor sequentially performs sample loading, rinsing and elution steps to obtain a solution to be tested; S200, the solution to be tested is used for LC-MS / MS detection. The technical problem solved by the present invention is that in the prior art, when the magnetic bead extraction method uses a single magnetic bead extraction, the detection of 8 catecholamines and their metabolites requires two sample pretreatments and two tests to complete, resulting in low throughput, poor recovery rate, and cumbersome operation, making it difficult to meet the needs of clinical high throughput.

The invention relates to the technical field of catecholamine detection, in particular to a high-sensitivity diagnostic kit for detecting catecholamine, which comprises air inlet baffles with filter meshes in the middle, the air inlet baffles are mounted on two sides of the lower end of a bottom component, and a storage component is mounted in the bottom component. One side of the upper end of the bottom assembly is rotationally connected with the top assembly through a connecting piece, the other side of the upper end of the bottom assembly is fixedly connected with a magnetic attraction block, one side of the bottom assembly is fixedly connected with a constant-temperature fan and an air guide bent pipe, and the other side of the bottom assembly is fixedly connected with an air guide plate. According to the invention, the flow direction of the air flow is controlled, the air flow can be used for driving the test tube to rotate, the contact probability of detection liquid in the test tube is enhanced, the detection efficiency and accuracy are improved, and meanwhile, in the process, negative pressure can be formed at the bottom of the kit, so that the kit is stably adsorbed on the detection table, the vibration is small, and the detection accuracy is improved. And the stability in the detection process is further improved.

The invention discloses an epoxy resin flame retardant based on a one-dimensional-two-dimensional layered structure nano hybrid as well as a preparation method and application of the epoxy resin flame retardant. According to the flame retardant, graphene oxide (GO) is used as a matrix, GO-APTES with larger interlayer spacing is obtained by modifying GO through APTES, and nanosheets are more dispersed. Then, dopamine is oxidized and self-polymerized under the alkaline condition to form a PDA coating, the surface of the beta-FeOOH nanorod is uniformly coated with the PDA coating, and beta-FeOOH is densely and stably loaded on GO-APTES nanosheets through the interaction of rich catechol / amine groups of the PDA coating and strong hydrogen bonds on the surface of GO-APTES, so that a GA (at) PDA (at) beta-FeOOH nanohybrid is prepared; the obtained GA (at) PDA (at) beta-FeOOH nano hybrid has good dispersibility in an epoxy resin matrix. When the flame retardant is applied to epoxy resin, the thermal stability and flame retardance of a composite material can be remarkably improved.

The invention discloses a hierarchical pore nanoreactor hydrogenation catalyst as well as a preparation method and application thereof, and belongs to the technical field of catalysts. Aiming at the problems of complex preparation and high cost of the existing nano reactor catalyst, poor structural adjustability of the synthesized nano reactor, insufficient consideration of dispersity of loaded active metal and the like, the method disclosed by the invention comprises the following steps: uniformly dispersing catecholamine substances, amino acid and a carrier template in an aqueous solution, adjusting the pH value to 9.0-11.0, and preparing the nano reactor catalyst. After fully stirring, carrying out suction filtration and drying to obtain a catecholamine-amino acid polymer modified material; dispersing in a solvent to form a dispersion liquid, adding a metal precursor into the dispersion liquid, uniformly stirring, and carrying out vacuum drying to remove the solvent; carrying out segmented roasting in an inert atmosphere; and finally, carrying out acid / alkali treatment to obtain the required hierarchical pore nano-reactor catalyst. The catalyst disclosed by the invention is simple to prepare, environment-friendly, low in cost and good in industrial application prospect, and is particularly suitable for hydrogenation of unsaturated organic acids.

The invention belongs to the technical field of biomedical detection, and relates to a kit and a method for simultaneously detecting catecholamine compounds, metabolites thereof and creatinine in human urine or plasma based on a derivatization method. A method for simultaneously detecting catecholamine compounds, metabolites thereof and creatinine in human urine or plasma based on a derivatization method comprises the following steps: respectively adding an internal standard working solution into a series of calibration products, a series of quality control products, a plasma sample and a urine sample, and uniformly mixing; adding a precipitant, uniformly mixing and centrifuging; taking supernate, carrying out nitrogen blowing and drying, adding a sample adjusting solution, and uniformly mixing; a derivatization reagent is added for a derivatization reaction, and a derivatization reaction solution is taken for LC-MS / MS detection analysis. The method breaks through the bottleneck of synchronous detection, realizes synchronous detection of catecholamine, metabolites thereof and creatinine in plasma and random urine for the first time, does not need multiple operations, obtains a whole set of core indexes required by clinical diagnosis at one time, and greatly improves the detection efficiency.

The invention relates to the technical field of electrochemical materials, and particularly discloses a nitrogen-doped yolk shell structure carbon sphere, an electrode, a supercapacitor and a preparation method. According to the invention, phenolic resin is taken as a core, catecholamine substances are taken as a shell precursor, and zinc salt is utilized to regulate and control the activity and fine structure of a carbon skeleton, so that an egg yolk shell structure with'core-shell-gap 'characteristics is prepared, and a mesopore-micropore interpenetrating multi-stage pore system is formed; the carbon sphere with the special structure has high specific surface area, good conductivity and high chemical stability, the maximum specific surface area is 567.04 m < 2 > / g, the specific capacitance of the supercapacitor prepared by taking the carbon sphere as an electrode is 225F / g when the current density is 0.5 A / g, the energy density is 16.7 Wh / kg, the cycle performance is excellent, the capacitance retention rate after 6000 cycles is 95.7%, and the specific capacitance retention rate is 95.7%. And the material shows extremely high application potential in the field of supercapacitors.

This invention provides a foam modifier, its preparation method, and its application. The foam modifier comprises a surfactant, nanoparticles, and water; the surfactant includes anionic gemini surfactants and anionic nonionic surfactants; the nanoparticles are catecholamine polymer particles. The structural formula of the anionic gemini surfactant is [insert structural formula here], and the structural formula of the anionic nonionic surfactant is R6-O-(R8-O). n -R7; The foaming agent described has good foaming properties and good stability under high-salt and high-calcium-magnesium conditions, and can be applied in high-salt reservoirs.

The application discloses a metal complex photosensitizer and a catecholamine coordination modification method and application thereof. The application utilizes a biological source catecholamine compound with redox activity (a characteristic structure unit can realize reversible conversion between phenol and quinone, and can generate a semiquinone free radical through disproportionation reaction, that is, QH2+Q ⇌ 2SQ·) to perform coordination modification on a metal complex photosensitizer, so that a new type of high-efficiency stable photosensitizer can be constructed. The application simultaneously solves three core pain points of existing photosensitizers, that is, low efficiency, instability and insecurity, pushes the metal complex photosensitizer to a brand-new performance platform, and is extremely likely to become a key breakthrough for promoting clinical transformation and application of the photosensitizer.

A purpose is to impart a function a protein having a β-sheet structure by using a material having a small molecular size. A modifying reagent for a protein having a β-sheet structure is provided, comprising a structure derived from a biomolecule and a group containing an aromatic ring, wherein the group containing the aromatic ring may has at least one selected from the group consisting of a benzothiazole group, a benzoxazole group, a benzimidazole group, and a naphthylazo group, each may have a substituent, the structure derived from the biomolecule includes a structure derived from one selected from a group consisting of an enzyme, an antibody, an antigen, a peptide, a polynucleotide, an oligonucleotide, a ligand, an enzyme substrate, a biotin, and a catecholamine.