41 results about "Calmodulin" patented technology

The invention discloses a neutralizing nucleic acid aptamer for targeted inhibition of a B cell activating factor and application of the neutralizing nucleic acid aptamer in treatment of systemic lupus erythematosus. The neutralizing aptamer disclosed by the invention can be used for efficiently inhibiting the combination of BAFF and specific receptors on a B cell membrane, including a BAFF receptor (BAFF-R), a human calcium regulatory cyclophilin ligand interaction molecule (TACI) and a B cell maturation antigen (BCMA), so that the proportion of plasma cells, plasma mother cells and hair growth center B cells is reduced, and finally, the generation and secretion of autoantibodies are reduced; the compound has a targeted therapeutic effect on systemic lupus erythematosus. Compared with an existing biological agent for neutralizing BAFF, the neutralizing nucleic acid aptamer has the advantages of being high in specificity, high in affinity, easy to synthesize, low in immunogenicity, low in cost, capable of being designed and programmed and the like, is a potential novel nucleic acid preparation for SLE targeted therapy, and has a good clinical transformation application prospect.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention discloses application of a plutella xylostella calmodulin inhibitor in prevention and treatment of lepidoptera pests. According to the invention, an inhibitor Cyclosporin A capable of inhibiting the activity of Plutella xylostella calcineurin is used for feeding Plutella xylostella larvae, and it is found that Cyclosporin A can cause the death of Plutella xylostella larvae and reduce the survival rate of the larvae. In addition, the Cyclosporin A treatment can prolong the pupation time of the plutella xylostella and the eclosion time of pupae, greatly reduce the pupation rate and the eclosion rate, and also can obviously reduce the egg laying amount of female plutella xylostella and the hatching rate of eggs. On the basis, the plutella xylostella can be prevented and controlled by utilizing the substance capable of inhibiting the activity of the calmodulin of the plutella xylostella. Meanwhile, the calmodulin is relatively conservative in the lepidoptera insects, so that substances capable of inhibiting the activity of the calmodulin of the plutella xylostella can also be used for preventing and treating other lepidoptera insects. The invention provides a new target for prevention and treatment of lepidoptera pests, and is beneficial to green prevention and treatment of lepidoptera pests.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

The application discloses an Aspergillus luchuensis YZ-1 and application thereof, and belongs to the field of microorganism safety.The application separates a new strain from a black tea in Guilin, Guangxi, identifies the strain by ITS, beta tubulin and calmodulin, and the identification result is Aspergillus luchuensis, and the preservation number is CCTCC NO: M 20221462.It is verified by experiments that the strain can inhibit various bacteria and fungi, the water extract and fermentation liquor of the strain can significantly inhibit the growth of Aspergillus flavus, the fermentation liquor has a strong inhibiting effect on the growth of Aspergillus flavus, and the strain has a strong adsorbing effect on aflatoxin B1.Therefore, the Aspergillus luchuensis disclosed by the application has important application value in preventing and controlling mold pollution in grain crops and food.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a calmodulin-binding protein CBP60g or a coding gene CBP60g thereof in control of clubroot, wherein an amino acid sequence of the calmodulin-binding protein CBP60g is shown as SEQ ID NO. 1. The application can restore the resistance of Arabidopsis thaliana to Plasmodiophora brassicae at high temperature by overexpressing CBP60g, and the same is verified in Brassica napus, and the normal growth, yield and quality of the Brassica napus are not affected, which indicates that the CBP60g gene can resist Plasmodiophora brassicae at high temperature, and increase the recovery of the immune response of crops to high-temperature combined stress, and provides a strategy for molecular breeding in response to climate change.

The invention provides a recombinant bovine lactoferrin peptide capable of reducing feedback inhibition on host bacteria and a construction method thereof in order to overcome the practical application problem of a genetic engineering method in the process of producing the bovine lactoferrin peptide at present. According to the invention, bovine lactoferrin peptide, calmodulin and enhanced green fluorescent protein are connected in series to form bovine lactoferrin peptide fusion protein, a pichia pastoris recombinant strain is constructed, and high-level expression of bovine lactoferrin peptide is realized. According to the method, positive charges of bovine lactoferrin are neutralized on the basis of calmodulin, toxicity of antibacterial peptide to a host is weakened, the tandem enhanced green fluorescent protein can emit green fluorescence under 488 nm exciting light, and real-time analysis of the expression level of bovine lactoferrin fusion protein is achieved. According to the method, by adopting a fusion expression technology, the toxicity to a host when the antibacterial peptide is produced by a genetic engineering method is overcome, so that the expression quantity is increased, and a foundation is laid for high-density fermentation production of the bovine lactoferrin peptide.

The invention discloses a medicago sativa MsCML69 gene for regulating and controlling salt tolerance and application of the medicago sativa MsCML69 gene. The nucleotide sequence of the MsCML69 gene is as shown in SEQ ID NO. 1; 1, and the corresponding amino acid sequence is as shown in SEQ ID NO. 2. The cDNA sequence of the calmodulin-like gene MsCML69 is cloned from medicago sativa, the obtained MsCML69 gene is connected with a plant expression vector to construct a recombinant expression vector, the constructed recombinant expression vector is used for transforming plant leaves, and finally transgenic plants are cultivated. Results show that overexpression of the gene can significantly improve the salt tolerance of the plant. The discovery of the MsCML69 gene provides a gene resource for cultivation of new stress-resistant germplasm of medicago sativa, the application range of the MsCML69 gene is widened, and the application of the MsCML69 gene has important production practice significance.

The invention belongs to the field of rice genetic breeding, and particularly relates to application of a rice calmodulin gene in regulation and control of rice high-temperature stress resistance. The invention discloses application of a rice calmodulin gene in regulation and control of high temperature stress resistance of rice, the nucleotide sequence of the rice calmodulin gene is shown as SEQ ID NO.1, CDS is shown as SEQ ID NO.2, and the amino acid sequence of protein coded by the rice calmodulin gene is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice calmodulin gene. The calmodulin gene is knocked out through a gene editing technology, the nucleotide sequence of the calmodulin mutant gene is as shown in SEQ ID NO.4, CDS is as shown in SEQ ID NO.5, the amino acid sequence of encoded protein is as shown in SEQ ID NO.6, and the high temperature sensitivity, heat resistance and relative setting rate of the mutant phenotype are remarkably reduced. The invention can provide new gene resources and theoretical basis for high-temperature resistance genetic breeding of rice.

The invention discloses a preparation method and application of whole-protein woven network hydrogel, and belongs to the technical field of biological materials. According to the preparation method disclosed by the invention, by designing a pseudo-cable hydrocarbon dimer (BXA ') and utilizing stimulation factors such as concentration, calmodulin (CaM), photoresponse CaM (hv), temperature and / or light to induce topological conversion of the pseudo-cable hydrocarbon dimer into a star-shaped molecule, intermolecular crosslinking is realized, and then spontaneous polymerization is performed to form a whole-protein woven network, so that the whole-protein woven network has dynamically adjustable mechanical properties; the ultrahigh-modulus full-protein woven network hydrogel with multiple stimulation responsiveness is realized. Further, the hydrogel can be used for immobilization and controllable release of enzymes (such as mannase). The whole-protein woven network hydrogel provided by the invention has the advantages of high biocompatibility, controllable structure, diversified response mechanisms and the like, and has wide application prospects in the fields of biomedicine, intelligent drug loading, tissue engineering and the like.

The invention discloses a BCG3973 gene knocked-down recombinant bacillus calmette guerin vaccine strain as well as a construction method and application thereof. According to the invention, a CRISPRi / dCas9th1 technology is utilized to block RNA polymerase movement in space through a compound formed by crRNA, dCas9 protein and sgRNA at a transcriptional level, so that the expression of the BCG3973 gene in the BCG vaccine is inhibited. After being treated by the recombinant bacillus calmette guerin bacterial strain, the immune-related pathways of macrophages, such as a cytokine-cytokine receptor action pathway, a Ca < + + > signal pathway and pathways such as PPAR and JAK-STAT, are obviously increased, and IL-1beta, TNF-alpha, antiviral protein and calmodulin with higher levels are expressed, so that the recombinant bacillus calmette guerin bacterial strain has stronger bactericidal ability on escherichia coli. Mouse in-vivo experiments show that the recombinant bacillus calmette-guerin bacterial strain has a very good protection effect on escherichia coli infection and endotoxemia caused by lipopolysaccharide, and shows higher survival rate and stronger capability of eliminating heterologous substance infection under attack of a lethal dose of Candida albicans.

The present invention relates generally to compounds of Formula (I), (II), (III), (IV), and (V), and pharmaceutically acceptable salts thereof, compositions and kits comprising the same, and methods of making and using the same. The compounds of the invention are effective in treating Diamond-Blackfan Anemia (DBA).

The invention discloses ultrasonic response ZnS (at) Lf piezoelectric nanoparticles as well as a preparation method and application thereof, and relates to the technical field of piezoelectric materials. The ultrasonic response ZnS (at) Lf piezoelectric nano-particle comprises a ZnS nano-particle and lactoferrin loaded on the surface of the ZnS nano-particle. According to the invention, lactoferrin is used as a potent brain-targeting ligand, and passes through a blood-brain barrier to be accumulated in brain parenchyma under the cell transfer action of brain capillary endothelial cells. ZnS nano particles release charges under the ultrasonic action, an electric signal activates a voltage-gated calcium ion channel and extracellular calcium ion influx, calcium ion / calmodulin kinase induces tyrosine hydroxylase (TH) phosphorylation, the activity of tyrosine hydroxylase is enhanced, tyrosine hydroxylase is a key enzyme for generating dopamine, and the activity of the tyrosine hydroxylase is enhanced. Therefore, more tyrosine in vivo is converted into neurotransmitter dopamine under the action of the enzyme. Finally, the regeneration of dopaminergic neurons is realized, so that the Parkinson's disease is effectively improved.

The invention discloses a protein nitrosation modification regulation compound as shown in a formula I as well as a preparation method and application of the protein nitrosation modification regulation compound. The compound disclosed by the invention can be used for simultaneously combining calcium / calmodulin dependent kinase II alpha (CaMKII alpha) and nerve type nitric oxide synthase (nNOS), promoting the interaction of the two proteins and up-regulating the nitrosation modification level of the CaMKII alpha, and can be used for preventing and / or treating diseases mediated by the CaMKII alpha.

Calcium / calmodulin-dependent protein kinase II (CaMKII) inhibitors and their use in treating or preventing cardiovascular diseases are disclosed.

The invention discloses a functional module for regulating drought and salt stress response of plants and application of the functional module. The functional module comprises a micro RNA member TaMIR7762, a target gene TaCML31 of the micro RNA member TaMIR7762 and a downstream MYB member TaMYB77 of the micro RNA member TaMIR7762. A precursor sequence of the micro RNA member TaMIR7762 is a sequence obtained by replacing T in SEQ ID NO: 1 with U, and a mature region sequence of the micro RNA member TaMIR7762 is a sequence obtained by replacing T in SEQ ID NO: 2 with U; the target gene TaCML31 is used for coding calmodulin protein, and the sequence of the target gene TaCML31 is shown as SEQ ID NO: 3; the sequence of the MYB transcription factor TaMYB77 is as shown in SEQ ID NO: 4, the expression of TaCML31 is negatively regulated through transcriptional cutting of the micro RNA member TaMIR7762, and the TaCML31 interacts with a DNA (Deoxyribose Nucleic Acid) binding structural domain in MYB of the TaMYB77 through an EF-Hand structural domain to jointly regulate the expression of a downstream stress defense gene. The invention shows that by silencing or inhibiting or reducing or knocking out the TaMIR7762 and overexpressing the target gene TaCML31 of the TaMIR7762 and the downstream MYB member TaMYB77, the drought tolerance and salt tolerance of wheat growth can be realized, so that the yield of wheat is greatly increased.

The invention discloses a ligand molecule, a nanowire as well as a preparation method and application of the nanowire, the ligand molecule is obtained by cross-linking trifluoromethylphenothiazine azide and a Linker molecule, and further, the ligand-protein interaction force of the trifluoromethylphenothiazine azide and calmodulin is utilized to successfully induce the assembly of the protein nanowire. According to the invention, the successful assembly of the nanowire is realized by regulating and controlling the size of the coupling molecule between the double-end ligands, the assembly method of the calmodulin CaM one-dimensional nanowire is expanded, and a new scheme is provided for the assembly of other protein nanowires with ligand molecules.

The invention provides a nano antibody targeting human transmembrane activator and calmodulin TACI (transmembrane activator and calmodulin phosphatase) interaction protein and application of the nano antibody. The antibody is derived from an alpaca heavy-chain antibody variable region. The anti-TACI single-domain antibody NB38 can be combined with human TACI with high affinity and specificity, can recognize recombinant TACI protein, can also recognize TACI with native conformation on the surface of a cell, and can partially block a BAFF / APRIL signal channel. Based on the nano antibody, gene engineering forms such as fusion protein, a chimeric antigen receptor, effector cells for expressing the chimeric antigen receptor, a bispecific cell connector and the like are constructed. The nano antibody can be further expanded into a coupled drug form. The product can be used for mediating directional recognition and killing of TACI positive cells, can be used as supplement and expansion of BCMA targeted therapy, and provides a new candidate technical scheme for targeted therapy of multiple myeloma and other TACI related diseases.

The invention relates to a chimonanthus praecox calmodulin protein CpCML45 and an application thereof. The CpCML45 gene is cloned from chimonanthus praecox, the maximum ORF frame of the CpCML45 gene is 627 bp, and 208 amino acids are encoded. According to the invention, a pCAMBIA-2301G-CpCML45 overexpression vector is constructed and is transferred into arabidopsis thaliana Col-0. Through overexpression of CpCML45, the leaf area of transgenic arabidopsis thaliana is significantly increased, the inflorescence is significantly increased, and the leaf area and the inflorescence are in direct proportion to the expression quantity. Through overexpression of CpCML45, drought tolerance and salt tolerance of transgenic arabidopsis thaliana are enhanced, but low temperature tolerance of the transgenic arabidopsis thaliana is reduced.

The invention provides antibodies, antibody-drug conjugates, bispecific T cell engagers (BiTEs), and chimeric antigen receptors (CARs) that target transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI). Such antibodies, antibody-drug conjugates, BiTEs, and CARs can be used, e.g., in methods for treating a cancer (e.g., multiple myeloma), an autoimmune disorder (e.g., characterized by a high titer of antibodies contributing to the disorder), or a plasma cell disease disorder (e.g., plasma cell dyscrasias) in a subject in need thereof.

The invention relates to a proton radiation brain injury tissue marker and application thereof, radiation brain injury differential metabolite markers comprise prostaglandin F2alpha, fructose-1, 6-diphosphate, dihydroxyacetone phosphate and inosine, and differential protein markers comprise fibroblast growth factor receptor 2 and calcium / calmodulin dependent protein kinase II. Differential metabolites and differential proteins are used as proton radiation brain damage biomarkers. According to the invention, the differential marker is used as a potential biomarker of radiation brain tissue injury, and demonstrative research is provided for multi-omics analysis of radiation brain injury and explanation of an action mechanism of radiation brain injury.

The invention belongs to the field of plant genetic engineering technology and disease control, and relates to calmodulin OsCML15 and application thereof in rice virus disease resistance.

The invention discloses a calmodulin electrochemical biosensor based on laser-induced graphene as well as a preparation method and application of the calmodulin electrochemical biosensor, and belongs to the technical field of electrochemical biosensors. The preparation method comprises the following steps: S1, depositing pyrrole-1-propionic acid on the surface of a laser-induced graphene electrode, and then dropwise adding a chlorinated 4-(4, 6-dimethoxy-1, 3, 5-triazine-2-yl)-4-methylmorpholine solution; s2, dropwise adding a phosphate buffer salt solution of target protein on the surface of the electrode deposited with pyrrole propionic acid, washing after incubation, drying, and dropwise adding a bovine serum albumin solution; and S3, dropwise adding a phosphate buffer salt solution of a protein antibody on the surface of the target protein modified electrode, incubating, washing, drying, dropwise adding a horse radish peroxidase labeled immune globulin solution, incubating, washing, and drying to obtain the calmodulin electrochemical biosensor. The sensor prepared by the method disclosed by the invention has relatively good stability and reproducibility while showing immediate, rapid and high-sensitivity detection.

The invention relates to the technical field of crop molecular biology, in particular to application of a CML47 calmodulin protein and a coding gene thereof in plant drought resistance, and the nucleotide sequence of the drought-resistant gene CML47 is as shown in SEQ ID NO.1; the amino acid sequence of the protein is as shown in SEQ ID NO. 2. After the CML47 gene is mutated through a CRISPR / Cas9 technology, the growth of a mutant plant under a drought treatment condition is obviously better than that of a wild plant type, which indicates that the drought resistance of the plant can be obviously improved after the gene is mutated. The invention provides a gene resource for cultivating and improving new varieties of drought-resistant plants; a resource is provided for deeply explaining a signal transduction network of a plant, and a theoretical basis is provided for explaining a molecular mechanism of the CML47 gene in corn drought stress signal response; compared with a traditional breeding mode, the provided drought response plant breeding method is short in breeding time and high in purposiveness, the breeding period is remarkably shortened, and the drought resistance breeding efficiency can be improved.

The present invention relates generally to methods, compositions and kits for treatment of ribosomal disorders and ribosomopathy, e.g. Diamond Blackfan anemia (DBA). In some embodiments, the invention relates to methods for the use of calmodulin inhibitors and calcium channel blockers for treatment of ribosomal disorders and ribosomopathy, e.g. Diamond Blackfan anemia (DBA).

The present invention discloses the use of KN-93 in the preparation of drugs for inhibiting porcine delta coronavirus, and relates to the technical fields of cell biology and virology. The present invention comprehensively utilizes technologies such as cell biology and virology, and finds that the calmodulin-dependent protein kinase II inhibitor KN-93 has the effect of inhibiting porcine delta coronavirus infection in vitro. First, KN-93 is subjected to cytotoxicity testing to determine the maximum safe concentration of the drug, and then fluorescent quantitative PCR (RT-qPCR), TCID 50 The in vitro inhibition of PDCoV infection by 10 μmol / L KN-93 was determined by ELISA and IFA, and it was found to significantly inhibit porcine deltacoronavirus. Therefore, KN-93 can be used to prepare drugs that inhibit porcine deltacoronavirus.

The present invention relates to methods, compositions and kits for treatment of ribosomal disorders and ribosomopathies, e.g. Diamond Blackfan anemia (DBA). In some embodiments, the invention relates to the use of novel classes of compounds, i.e. inhibitors of RSK (p90S6K); inhibitors of p70S6K; and inhibitors of rps6, to treat ribosomal disorders and ribosomopathies. In some embodiments, the invention relates to the use of specific Chk2 inhibitors and to the use of specific phenothiazine derivatives to treat ribosomal disorders and ribosomopathies, e.g. DBA.

The present invention relates generally to compounds of Formula (I), (II), (III), (IV), and (V), and pharmaceutically acceptable salts thereof, compositions and kits comprising the same, and methods of making and using the same. The compounds of the invention are effective in treating Diamond-Blackfan Anemia (DBA).

To provide an oligonucleotide enabling accurate grasp of the initial onset of Welsh onion white rot and a method for detecting both group A and group B strains of Sclerotium cepivorum.SOLUTION: The detection method according to the present invention uses an oligonucleotide of 15-30 base-length that is capable of annealing to any one region, or a complementary region thereof, selected from gene regions of glyceraldehyde-3-phosphate dehydrogenase (G3PDH), heat shock protein 60 (HSP60), and calmodulin (CaM) of plant-pathogenic Ascomycota, the region being conserved in both group A and group B strains of Sclerotium cepivorum and exhibiting polymorphisms in Botrytis, Dumontinia, Macrophomina, Sclerotinia, and Sclerotium., Sclerotinia, and Sclerotium.SELECTED DRAWING: Figure 7