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4 results about "Ribosomal protein" patented technology
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A ribosomal protein (r-protein or rProtein) is any of the proteins that, in conjunction with rRNA, make up the ribosomal subunits involved in the cellular process of translation. A large part of the knowledge about these organic molecules has come from the study of E. coli ribosomes. All ribosomal proteins have been isolated and many specific antibodies have been produced. These, together with electronic microscopy and the use of certain reactives, have allowed for the determination of the topography of the proteins in the ribosome. E. coli, other bacteria and Archaea have a 30S small subunit and a 50S large subunit, whereas humans and yeasts have a 40S small subunit and a 60S large subunit. Equivalent subunits are frequently numbered differently between bacteria, Archaea, yeasts and humans. More recently, a near-complete (near)atomic picture of the ribosomal proteins is emerging from the latest high-resolution cryo-EM data (including PDB ID: 5AFI).
Expression cassettes, expression vectors, genetically engineered strains and their applications in the preparation of malonic acid
This invention provides an expression cassette, an expression vector, a genetically engineered strain, and their application in the preparation of malonic acid. The expression cassette includes expression cassette 1, expression cassette 2, and expression cassette 3. Expression cassette 1 includes the gene for the mitochondrial 37S ribosomal protein mS47; expression cassette 2 includes the ACC1 gene, which incorporates a mitochondrial localization signal encoding gene at its 5' end; and expression cassette 3 includes the upstream homologous arm of an endogenous ACC1 gene, which incorporates a weak promoter at its 5' end. This invention, through the expression cassette, can promote an increase in the levels of mS47 and ACC1 in mitochondria, thereby promoting the synthesis pathway of acetyl-CoA-malonyl-CoA-malonic acid (ACEA), while simultaneously reducing the level of endogenous ACC1 in the cytoplasm, avoiding competitive effects on the mitochondrial ACEA synthesis pathway, and ultimately effectively increasing malonic acid production.
Owner:WANHUA CHEM GRP CO LTD
Recombinant polyepitopic immunogenic polypeptides derived from pratylenchus penetrans and uses thereof
A recombinant multi-epitope immunogenic polypeptide derived from *C. bancroftian* and its applications are disclosed. The amino acid sequence of the recombinant multi-epitope immunogenic polypeptide includes a fragment of the *Mycobacterium tuberculosis* 50S ribosomal protein L7 / L12, a cytotoxic T lymphocyte epitope, a helper T lymphocyte epitope, and a linear B cell epitope, formed by tandem linkers. The nucleotide coding sequence of the polypeptide is also disclosed. This invention further discloses a recombinant expression vector containing the nucleic acid molecule, a host cell containing the recombinant expression vector, an immunogenic composition containing the recombinant multi-epitope immunogenic polypeptide, and a method for preparing the recombinant multi-epitope immunogenic polypeptide. Immuninformatics analysis results show that the polypeptide is antigenic, non-sensitizing, and non-toxic; structural prediction, molecular docking, and computer immunosimulation results suggest its application potential as a candidate immunogenic polypeptide related to *C. bancroftian*.
Owner:HANGZHOU DIANZI UNIV
Process for the preparation of 30s ribosomal protein s1 and its use as an antibacterial potentiator
ActiveCN118725042BRibosomal protein E-L30Acinetobacter species
The application provides a preparation method of 30S ribosomal protein S1 and use of the 30S ribosomal protein S1 as an antibacterial synergist, and belongs to the technical field of biological medicines. The 30S ribosomal protein S1 in the application is a bioactive protein produced by Acinetobacter bacteria, and has the effect of enhancing the antibacterial capacity of antibiotics. In the process of being combined with streptomycin sulfate, the 30S ribosomal protein S1 greatly enhances the inhibiting capacity of streptomycin sulfate on avian pathogenic E.coli without showing the effect of inhibiting bacteria itself, which lays a foundation for future practical application, and also plays a reference role for the development of new antibacterial synergists.
Owner:JILIN UNIVERSITY
Ribosomal protein n' mutant and application thereof
The application belongs to the technical field of genetic engineering, and particularly relates to a ribosomal protein N' mutant and application thereof. The ribosomal protein N' mutant is subjected to Y190D mutation. When the ribosomal protein N' expressed by a production strain is subjected to Y190D mutation, the production of LNT II is increased by 28.6%, the production of LNnT is increased by 50%, the production of LNT is increased by 92.3%, the production of 3'-SL is increased by 13.8%, and the production of 6'-SL is increased by 20%. It can be seen that after the 190th amino acid of PriA is changed from tyrosine to aspartic acid, the production capacity is improved. When the ribosomal protein N' mutant is applied to an Escherichia coli for producing human milk oligosaccharides, the production of human milk oligosaccharides is significantly improved.
Owner:TIANJIN UNIV OF SCI & TECH