188 results about "Protein family" patented technology

The present invention relates to the design of trimeric polypeptides using polypeptide structural elements derived from the tetranectin protein family, and their use in rational de novo design and production of multi-functional molecules including the application of the multi-functional molecules in protein library technology, such as phage display technology, diagnostic and therapeutic systems, such as human gene therapy and imaging. The trimeric polypeptides being constructed as a monomer polypeptide construct comprising at least one tetranectin trimerising structural element (TTSE) which is covalently linked to at least one heterologous moiety, said TTSE being capable of forming a stable complex with two other TTSEs; or as an oligomer which is comprised of two monomer polypeptide constructs as mentioned above, and which comprises three TTSEs or a multiplum of three TTSEs, or which is comprised of three monomer polypeptide constructs.

Bispecific single chain antibody molecules are disclosed which may be used to advantage to treat various forms of cancer associated with the overexpression of members of the EGFR protein family.

Anti-EGFR family member antibodies and bispecific antibodies comprising one or more anti-EGFR family member antibodies are disclosed. These antibodies can be used to advantage to specifically target forms of cancer associated with the overexpression of members of the EGFR protein family.

Methods useful in constructing libraries that collectively display members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.

The invention concerns the identification and use of neogenin receptor-binding domains of members of the repulsive guidance molecule (RGM) protein family as well as polypeptide fragments derived therefrom. The inventive domains, i.e. peptide fragments are suited as agents for the active or passive immunization of individuals as well as diagnostic and therapeutic agents for use with diseases or pathological conditions in whose origin or progression, a member of the RGM family and a cellular receptor assigned to this molecule are involved. The invention also concerns monoclonal and polyclonal antibodies directed against the inventive binding domains and against the polypeptides derived therefrom, and to method for producing the inventive domains, polypeptides and antibodies.

The invention provides processes and compositions for enhancing the immunogenicity of TAP-1 expression-deficient cells by increasing the presentation of MHC Class I surface molecules for detection by cytotoxic T-lymphocyte cells through increased TAP-1 expression, which comprises administering to the TAP-1 expression-deficient cells a TAP-1 expression increasing amount of a bio-acceptable substance that promotes transcription of TAP-1 gene in the cells to cause enhanced MHC Class I surface expression of the cells. The bio-acceptable substance may be a histone H3 deacetylase inhibitor, such as trichostatin A, a transcriptional co-activator having intrinsic histone acetyl transferase activity or a histone acetyl transferase comprising at least one member of the CBP/p300 protein family. The process and compositions increase the immunogenicity of the target cells to enhance their destruction by cytotoxic lymphocytes.

A genetic locus and corresponding family of proteins associated with regulation of immune development, function, and cell survival are provided. The locus comprising the TIM family is genetically associated with immune dysfunction, including atopy, autoimmunity, inflammatory bowel disease, dysplasia, and susceptibility to blood-bourne infectious diseases. Polymorphisms in the human TIM-1 gene and exposure to Hepatitis A Virus (HAV) are shown to be associated with protection from the development of atopy.

Nucleic acids encoding SOC/CRAC calcium channel polypeptides, including fragments and biologically functional variants thereof and encoded polypeptides are provided. The nucleic acids and polypeptides disclosed herein are useful as therapeutic and diagnostic agents. Agents that selectively bind to the foregoing polypeptides and genes also are provided.

The invention discloses a rice leaf color control gene heme oxygenase2 (HO2) and an application thereof. A gene which is cloned from a rice leaf control mutant ho2 and is proved to have the effect of controlling the color of a rice leaf is named after HO2. A transgene functional complementation assay proves that the HO2 is the gene which controls the color of the rice leaf. Comparative analysis on amino acid sequences shows that HO2 protein belongs to a hemeoxygenase protein family. Phenotype observation and biophysical analysis prove that the cloned gene has the effects of regulating and controlling the development of chloroplasts and then controlling the color of the leaf. The invention also discloses an application of the rice leaf color control gene HO2 in fine seed breeding and cross breeding by taking a green yellow leaf character as a marker character by using genetic engineering. The rice leaf color control gene HO2 is obtained by using a map-based cloning technology, and the effects of the gene is tested through the transgene functional complementation test. Therefore, the rice leaf color control gene HO2 has the effect of ensuring rice to keep an appropriate leaf color, so that the photosynthetic efficiency of the rice is improved, and finally the rice yield can be improved.

The invention discloses a novel recombinant insect resistant protein, as well as a preparation method and an application thereof, relates to the fields of insect resistant protein and biosafety and solves the existing problem of the prior art. The method comprises the following steps: predicting a Cry protein family partial member to determine a directional transformation area from the point of protein natural evolution through a pMAL algorithm; selecting constructed protein by using an MP method to construct 64 recombinant proteins; and synthesizing an insect resistant gene NGc through insect resistant activity test and codon modification, and expressing to obtain the novel recombinant insect resistant protein NGc. The NGc is constructed to a plant expression carrier by taking ubi as a promoter and bar as a selection marker; the insect resistant protein NGc is converted to a corn selfing line through agrobacterium mediated infection, a transgenic offspring is a corn plant with high resistant immune level and with the insect resistant effect better than the trans-CrylAb, CrylGc through an indoor/field insect resistant authentication. Compared with the non-transgenic rice main cultivar Jijing 88, the transgenic rice has an obvious high striped rice borer resistance characteristic; and the recombinant insect resistant protein has high-efficiency expression in a monocotyledon and high lepidopteron resistant biological characteristic.

The invention discloses gossypium hirsutum GhB2 protein as well as an encoding gene and application thereof. The GhB2 protein provided by the invention is as shown in (1) or (2): (1) proteins as shown in SEQ ID No. 2; (2) proteins obtained through substitution and/or deficiency and/or addition of one or more amino acid residues on amino acid sequences shown in the SEQ ID No. 2, and with the same functions. According to the gossypium hirsutum GhB2 protein and the encoding gene and application thereof disclosed by the invention, sequencing of a transcription group of Chinese planted cotton KV3 is used as a foundation, cotton GhB2 genes are cloned, typical DCD ultra-protein family genes are obtained, procedure death of cells is mediated, through gene silencing and overexpression analysis, the effects of the gossypium hirsutum GhB2 protein in verticillium wilt resistance for Gossypium hirsutum are discussed, and candidate genes are provided for subsequent cultivation of disease-resistant varieties through genetic engineering means.

The present invention relates to peptide, polynucleotide and fusion proteins for use as inhibitors in hemostasis. These inhibitors are members of the family of proteins bearing a collagen-like domain and a globular domain. The inhibitors are useful for promoting blood flow in the vasculature by reducing thrombogenic and complement activity. The inhibitors are also useful for pacify collagenous surfaces and modulating wound healing.

The invention provides a dust mite allergen and an application thereof. The dust mite allergen belongs to the TEF (translation elongation factor) protein family, can be used for diagnosing, treating and preventing allergic diseases, particularly dust mite allergic diseases, and is particularly used for diagnosing, treating and preventing the allergic disease caused by a thirty-fifth component of the dust mite allergen. Recombinant protein of the dust mite allergen is prepared through gene cloning and protein expression, the protein purity is high, the specificity is better, and the yield is high. The dust mite allergen is used for preparing a reagent for diagnosing, preventing or treating the allergic disease caused by the dust mite allergen, has the characteristics of high specificity and low cost, and particularly can be used for effectively diagnosing whether a patient is allergic to the thirty-fifth component of the dust mite allergen.

The invention relates to an anther development control gene and use thereof in realizing male sterility in Arabidopsis thaliana. An Arabidopsis thaliana male sterile mutant ahl16 is screened out from T-DNA-inserted mutant population, a gene AHL16 controlling the fertility of Arabidopsis thaliana is cloned and identified, the nucleotide sequence of the gene AHL16 is represented by SEQ ID N0.1, and genetic complement experiments prove that the AHL16 gene in a wild type can restore the male sterile phenotype. Amino acid sequence analysis and subcellular localization experiments indicate that the AHL16 gene codes proteins of an AT-hookmotif nuclear localized protein family and is located in the nucleus. In Arabidopsis thaliana, the mutation of the gene leads to complete male sterility. The gene has a very important application value in aspects of explanation of influences of the growth of the inner layers of the outer walls of microspores and the structures of the inner walls on the fertility of plants and improvement on yield by hybrid seed production.

The present invention relates to a method for identifying compounds that modulate the activity of sirtuin deacetylase protein family members. Compounds of the invention are identified by designing or screening for a compound which binds to at least one amino acid residue of the newly identified nicotinamide inhibition and base exchange site of Sir2 and testing the compound for its ability to modulate the activity of the Sir2 protein. Compositions and methods for preventing or treating diseases or disorders associated with Sir2 are also provided.

The present invention provides compositions and methods that enhance cell survival. Such compositions feature chimeric polypeptides that include at least a GM-CSF receptor ligand and an anti-apoptotic moiety (e.g., a Bcl-2 protein family member). In one embodiment, the chimeric polypeptide is a GM-CSF-Bcl-xL chimeric polypeptide. The invention further includes methods of using chimeric polypeptides to enhance cell survival or inhibit cell death in a cell at risk of cell death.

Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospholipids, and related unnatural polymers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.