9results about How to "Enhance cell viability" patented technology

The present disclosure relates to microorganisms comprising a protein encoded by an exogenous ftsN gene, and methods of producing L-amino acids using the same.

The invention relates to the technical field of medical health care products, in particular to an anticancer composition containing phellinus igniarius extract and total ginsenoside and application of the anticancer composition. The anticancer composition comprises a phellinus igniarius extract and total saponins of ginseng. The mass ratio of the phellinus igniarius extract to the total ginsenoside is 1: (0.5-2); the phellinus igniarius extract is obtained by taking artificially cultivated phellinus igniarius as a raw material through ultrasonic-assisted extraction and reflux extraction in stages; the extraction solvent is selected from water or an ethanol water solution with the ethanol concentration not exceeding 40%. The anticancer composition provided by the invention has remarkable in-vitro anticancer activity and low toxicity to normal cells.

This invention provides a kit, method, and single-cell library construction method for the dissociation of marine animal tissues. The dissociation method includes: S1) dissociating marine animal tissue samples at 8℃~12℃ using a cell dissociation buffer to obtain a single-cell suspension; S2) centrifuging the single-cell suspension and washing the single-cell precipitate obtained after centrifugation with a cell resuspension buffer to obtain single cells from the marine animal tissue sample; wherein, the cell dissociation buffer comprises a protease; the protease is Bacillus subtilis protease A; the concentration of sodium ions in the cell resuspension buffer is 300-800mM, and the concentration of potassium ions is 5-20mM.

This invention provides a benzothiadiazine 1,1-dioxide compound, its preparation method, and its applications. The general structural formula of the benzothiadiazine 1,1-dioxide compound is shown in Formula (I), or its isomers, or pharmaceutically acceptable salts, esters, or prodrugs thereof. The benzothiadiazine 1,1-dioxide compound of this invention has a novel structure, can selectively inhibit HDAC6 enzyme, has a strong protective effect on nerve cells, low toxicity, and low potential cardiotoxicity, and is expected to be used as a neuroprotective agent for the treatment of neurodegenerative diseases.

The application belongs to the technical field of biological medicine, and particularly relates to a thymus tissue dissociation reagent, a kit and application thereof in obtaining thymus epithelial cells. The thymus tissue dissociation reagent at least comprises collagenase D, dispersinase II, deoxyribonuclease I, dissociation enzyme and a dissolving reagent; and collagenase II and / or collagenase IV and / or trypsin. The cell survival rate of the thymus tissue dissociation reagent after dissociating the thymus tissue is maintained at a high level of 95.7% to 99.6%, which is significantly higher than that of the prior art; and after sorting, the number of thymus epithelial cells enriched and obtained from each gram of thymus tissue is 1.38e 5 ~1.06e 6 , and the success rate of enriching and obtaining thymus epithelial cells is as high as 95%, which is significantly higher than that of the prior art; and the proportion of epithelial stem cells in the thymus epithelial cells is 65.7%, which is significantly higher than that of the prior art.

The invention discloses an application of marine-derived alkaloid in preparation of an anti-cholestasis medicine, and belongs to the field of natural medicines. The invention discloses an application of indolediketopiperazine alkaloid 12R, 13S-Dihydroxyfumitorgin C (DHFC) or a pharmaceutically acceptable salt of the indolediketopiperazine alkaloid in preparation of an agonist of a peroxisome proliferator-activated receptor delta (PPAR delta), and an application of the indolediketopiperazine alkaloid 12R, 13S-Dihydroxyfumitorgin C (DHFC) or the pharmaceutically acceptable salt of the indolediketopiperazine alkaloid in preparation of an anti-cholestasis medicine, and the indolediketopiperazine alkaloid 12R, 13S-Dihydroxyfumitorgin C (DHFC) or the pharmaceutically acceptable salt of the indolediketopiperazine alkaloid has a protective effect on cholestasis by activating the PPAR delta. DHFC is in high-affinity binding with an LBD region of PPAR delta, the transcriptional level of downstream genes of PPAR delta is increased, the affinity KD of DHFC to PPAR delta protein is 153nM, and the DHFC plays a synergistic treatment role in multiple links of improving bile acid homeostasis, inhibiting inflammation, relieving liver fibrosis and the like. The invention provides a new lead compound for the development of anti-cholestasis drugs, and has important significance for the development of Chinese marine-derived drugs. Formula (I)

The application discloses a kind of pyranose derivatives represented by the following general formula I, its stereoisomer, pharmaceutically acceptable salt, crystalline hydrate or solvate, its preparation method, the pharmaceutical composition for protecting the compounds and its application in central nervous system diseases and inflammatory diseases etc..According to the pyranose derivative of the application, the cell viability of A beta 1-42 induced cell damage model is significantly improved, the cell viability of 6-hydroxydopamine induced cell damage model is improved, the cell viability of oxygen-glucose deprivation / reoxygenation cell damage model is improved, the level of lipopolysaccharide induced cell inflammatory factor is inhibited, etc.The pharmacological effects have good clinical application prospect.

The present application belongs to the field of biomedical and cosmetic technology, and particularly relates to the application of protein AMBP in the preparation of a preparation for promoting the activity of hair follicle cells or for preventing and treating alopecia. The present application first discovers that the protein AMBP has the activity of regulating the hair follicle hair papilla cells, hair matrix cells and outer root sheath cells, can promote the proliferation of the hair papilla cells, the hair matrix cells and the outer root sheath cells, and improve the ability of the outer root sheath cells to remove active oxygen; also has the effects of promoting the hair follicle cycle transition, promoting hair growth and hair thickening. Therefore, the protein AMBP can be applied to the preparation of a preparation for treating alopecia.

This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a food-medicine homology composition and its use in the preparation of a treatment for lung injury. The food-medicine homology composition of this invention comprehensively considers the overall state of the body and the intrinsic physiological functions of the lungs. Its main active ingredients are lung-protecting food-medicine homology materials and small-molecule collagen peptides. Specifically, *Siraitia grosvenorii* (monk fruit) and *Lilium brownii* (lily bulb) are the principal ingredients, while *Eriobotrya japonica* (loquat leaf), *Platycodon grandiflorus* (platycodon root), *Lonicera japonica* (honeysuckle flower), and *Morus alba* (mulberry leaf) are the assistant ingredients. The adjuvant ingredients include *Dioscorea opposita* (Chinese yam), *Codonopsis pilosula* (codonopsis root), *Astragalus membranaceus* (astragalus root), *Pyrus pyrifolia* (snow pear), *Tremella fuciformis* (white fungus), and olive. These ingredients have the effects of clearing heat and moistening the lungs, invigorating qi and strengthening the spleen, and nourishing yin and moistening dryness. Combined with small-molecule collagen peptides, it comprehensively regulates lung tissue, helps improve lung function, promotes lung repair, and achieves lung-protecting effects, making it suitable for the treatment of lung injury.