237 results about "Cell survival rate" patented technology

The invention relates to the field of biological medicine, and discloses a rhizoma polygonati-fructus lycii heterozygous exosome, a preparation method and application thereof and an antioxidant composition. The hybrid exosome comprises miRNA-156a (micro Ribonucleic Acid) and miRNA-396b (micro Ribonucleic Acid), and the expression quantity ratio of the miRNA-156a to the miRNA-396b is (1.2 to 1.8): 1; the heterozygous exosome is loaded with a polygonatum kingianum characteristic active component and a Chinese wolfberry fruit characteristic active component. The rhizoma polygonati-fructus lycii heterozygous exosome is good in biocompatibility and capable of remarkably improving the cell survival rate, and brand-new and efficient raw materials and technical supports are provided for development of anti-aging and anti-oxidation functional products.

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention relates to the technical field of microfluidics, in particular to a high-throughput and high-efficiency cell extrusion microfluidic system and a particle introduction method. And a closed-loop control system integrating real-time cell deformation sensing, dynamic parameter decision and physical extrusion and alternating electric field cooperative application is constructed. The chip is provided with an inlet shunting device, a micro-channel and a channel structure of a microelectrode. The method comprises the steps of real-time deformation image acquisition and analysis and dynamic extrusion parameter decision and cooperative application. The invention provides a high-flux and high-efficiency cell extrusion microfluidic system and a particle introduction method, and aims to solve the problems that the drug loading efficiency and the survival rate are difficult to consider due to heterogeneity in the existing cell extrusion technology and a physical extrusion and electroporation coordinated regulation strategy is lacked, so that personalized precise treatment of cells is realized, and the cell quality is improved. The drug loading efficiency, the cell survival rate and the high throughput are obviously improved.

The invention discloses an application of an antibacterial peptide and an application of the antibacterial peptide in preparation of antibacterial drugs for treating carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the antibacterial peptide is characterized in that the sequence is w-r-r-w-k-r-w-w-r-r, and the sequence is shown in the description. All amino acids are D-type amino acids; the antibacterial peptide can be used as an antibacterial substance in wash supplies, food preservative additives, medical consumable antibacterial agents and cosmetics, can be synthesized through an Fmoc solid-phase chemical method, and is low in synthesis difficulty and good in in-vivo antibacterial activity. Particularly, the antibacterial peptide has broad-spectrum killing activity against carbapenem pseudomonas aeruginosa, klebsiella pneumoniae (ATCC700603), escherichia coli (ATCC25922), staphylococcus aureus and methicillin-resistant staphylococcus aureus, the cell survival rate of the antibacterial peptide within the range of medium and low concentration (2 mu g / mL-32 mu g / mL) reaches half or more, the toxicity is small, the toxicity is almost avoided, the operability is high, and the antibacterial peptide can be widely applied to the field of medical application. The cost is low.

The invention provides an intelligent decision-making method and system for free flap riser anastomosis based on hemodynamics. According to the method, firstly, the blood flow direction, the flow speed and the blood vessel wall tension parameter of a target blood vessel are collected, and a dynamic correlation model of intravascular fluid mechanics and external tissue deformation is established; a three-dimensional blood flow pressure gradient map is generated based on the model, then, a system automatically screens an anastomosis path meeting a blood flow resistance threshold value and the endothelial cell survival rate requirement, and the optimal anastomosis pose is determined by analyzing the blood vessel wall elasticity matching degree and the turbulence generation probability. Then, guiding the accurate adjustment of the clamping direction of the blood vessel and the suture needle pitch in the operation, ensuring that the pressure of the lumen of the anastomosed blood vessel is kept balanced when the anastomosed blood vessel is axially stretched, meanwhile, effectively inhibiting local vortex, and remarkably improving the long-term smoothness of the anastomosis of the blood vessel. According to the method, mechanical precise matching and blood flow stable control of free flap vascular anastomosis are achieved, and the postoperative risk is remarkably reduced.

The invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and application of the culture medium. The culture medium contains inorganic salt, amino acid, vitamins, energy substances, a buffer substance, an antioxidant, sterol, polyamine, an additive and an indicator, and the antioxidant comprises dihydrolipoic acid and S-acetyl-L-glutathione. According to the invention, the culture medium for serum-free suspension culture of the BHK-21 cells, which is clear in components and remarkable in culture effect, can be provided. The added dihydrolipoic acid and S-acetyl-L-glutathione are combined as antioxidants of the culture medium, so that the survival rate of BHK-21 cells cultured in the culture medium is improved while the oxidation resistance of the serum-free suspension culture medium is improved.

The invention discloses a gelatin microcarrier lysate as well as a preparation method and application thereof. The gelatin microcarrier lysis buffer comprises lyase, a cell protective agent, a cell energy metabolism protective agent, an antioxidant, a buffer solution and a chelating agent. The gelatin microcarrier lysate has extremely strong protectiveness to cells, abandons damaging trypsin and EDTA, and innovatively adds a cell protection combination of a cell protective agent L-arginine, a cell energy metabolism protective agent sodium pyruvate and an antioxidant vitamin C, so that the cell survival rate (gt; 98%) and the retention rate (gt; the method is high in cell yield, good in safety and wide in range.

The invention discloses application of a 3D hypoxia mesenchymal stem cell derived exosome preparation in preparation of an immune cell proliferation inhibitor or a medicine for treating acute myocardial infarction, belongs to the technical field of biology, and solves the problems of low cell survival rate and immunological rejection in application of a cell treatment method in the prior art. The 3D hypoxia mesenchymal stem cell derived exosome is used as a medium for treating acute myocardial infarction, and the problem of immunological rejection can be solved.

The invention discloses a preparation method and application of cubilose peptide. The amino acid sequence of the cubilose peptide is KPAPPKPEPK. The cubilose peptide prepared by the invention has strong ultraviolet damage resistance, and can improve the cell survival rate, improve the antioxidant enzyme level in serum, scavenge free radicals and reduce the inflammatory factor level in skin tissues. When the cubilose peptide is used for developing anti-ultraviolet damage products, the efficacy of cubilose can be deeply excavated, and the quality and economic value of cubilose products are improved.

The invention discloses composite hydrogel and application of the composite hydrogel in preparation of a diabetes wound repair dressing. The gel comprises Metformin (Met) loaded Mg-MOF / Met nano-particles, a hydrogel carrier and a hydrogel carrier, wherein the Mg-MOF / Met nano-particles are prepared from the following components of Mg-MOF / Met nano-particles; the hydrogel network is formed by cross-linking chitosan (CA) modified by arginine and oxidized dextran through a Schiff base reaction; wherein the Mg-MOF / Met nano particles are uniformly dispersed in the hydrogel network, and the Mg-MOF / Met nano particles are uniformly dispersed in the hydrogel network. An animal model verifies that after 14 days, the healing rate reaches 92.1%, the antibacterial rate is larger than 95%, the ROS clearance rate is 85.2%, the cell survival rate is larger than 95%, and remarkable application prospects are shown in the field of diabetes wound repair.

The application discloses a kind of live cell dynamic monitoring method, comprising the following steps: obtaining the cell scanning picture group that live cell grown in culture vessel is photographed under specified condition, and the picture is preprocessed;Cell scanning picture group after pre-processing is input into trained cell identification model, and output cell growth state chart group;Cell growth state chart group is input into trained survival rate calculation model, and the survival rate of cell in each cell growth state chart is output;The cell survival rate numerical value obtained is drawn into cell survival rate-time curve chart;The cell survival rate-time curve chart generated is combined with nonlinear regression statistical method, and the half inhibitory concentration value of the efficacy of specified parameter calculation is selected.The growth state of cell under specified condition is monitored, and the survival rate of cell and the half inhibitory concentration value of the efficacy are calculated based on these data;It can significantly improve the efficiency and accuracy in the field of drug research and development, drug screening, treatment scheme effect, etc.

The invention relates to a non-gadolinium magnetic resonance contrast agent and a preparation method thereof, and belongs to the field of contrast agents. The invention aims to provide a non-gadolinium contrast agent with high biological safety aiming at the risks of renal fibrosis and cerebral deposition existing in a gadolinium-based magnetic resonance contrast agent. The invention provides a phosphate group modified non-gadolinium magnetic resonance contrast agent. The phosphate group modified non-gadolinium magnetic resonance contrast agent consists of a ligand of a phosphate group substituted trans-cyclohexanediamine tetraacetic acid derivative (tmPCDTA or tqPCDTA) and metal ions Fe < 3 + > or Mn < 2 + >, the contrast agent disclosed by the invention is high in biological safety: endogenous Fe < 3 + > / Mn < 2 + > is used for replacing gadolinium, and cell experiments prove that the survival rate of 4T1 cells is not influenced at the concentration of 500 mu M; clinical applicability is high, kidney excretion is rapid, bladder signals are enhanced 3-5 minutes after mouse intravenous injection, and liver retention is avoided.

The invention provides a method for improving the success rate of single cell culture under external stimulation, belongs to the field of cell culture, and can solve the problem that the contradiction between a Raman spectrum signal and the survival rate can be formed by improving laser energy in the existing single cell culture. The method comprises the following steps: preparing a working solution containing an allogenic material; pretreating an experimental strain by using a culture medium containing the allogenic material to obtain a cell suspension; introducing the cell suspension into chambers of a micro-fluidic chip, and adjusting the cell suspension until each chamber contains at most one cell; in the presence of the allogenic material, culturing the single cell; wherein the allogenic substance is selected from one or more of an antioxidant substance, an anti-radiation substance, an anti-apoptosis substance and a repair promoting substance. According to the method, the exogenous material is added into the culture medium, and the micro-fluidic chip with a plurality of chambers is adopted for single cell culture, so that the survival rate and the clone formation rate of the single cells after Raman sorting can be remarkably improved, meanwhile, the advantage of low background interference is kept, and the survival rate of the single cells is improved.

The invention belongs to the technical field of antiviral drugs, and relates to application of a pyrimidine derivative as a coxsackie virus CVB3 inhibitor. The anti-CVB3 activity research experiments of several pyrimidine derivatives show that the pyrimidine derivatives inhibit the cytopathic effect (CPE) generated by CVB3 on host cells Hep-2, enhance the cell survival rate and reduce the progeny virus yield; the inhibitory activity of the pyrimidine derivative on CVB3 shows that the pyrimidine derivative has application potential in preparation of anti-CVB3 virus drugs.

The invention belongs to the technical field of compound application, and particularly relates to coriaria lactone compounds, a preparation method thereof and application of the coriaria lactone compounds in oxidative stress resistance. The coriaria lactone compound can significantly inhibit oxidative stress damage of SH-SY5Y cells, and the cell survival rate of the coriaria lactone compound is increased to 79.6%. Meanwhile, the nematode anti-aging activity is achieved, generation of nematode lipofuscin is inhibited, and the highest inhibition rate is 45%. In an extreme oxidative stress experiment, the service life of the nematodes is prolonged, so that the coriaria lactone compound has an application prospect of treating and resisting aging diseases.

The application discloses application of mesenchymal stem cell spheroids in preparation of a preparation for treating osteoarthritis, and relates to the technical field of cell therapy and osteoarthritis treatment, and specifically comprises mesenchymal stem cell spheroids with an average diameter of 120-180 mu m and a solution containing 0.5%-10% human serum albumin. The cells are prepared into 120-180 mu m cell spheroids, and the solution containing specific concentration human serum albumin is used as a carrier, so that the cell survival rate is stabilized at more than 90%, and high-activity delivery is realized; the optimized size ensures smooth injection and long-term retention in the joint cavity, and overcomes the shortcoming that single cells are easily removed; after joint cavity injection, the preparation can synergistically promote bone reconstruction and cartilage repair, and produces clear curative effects such as bone mass increase of more than 30%, cartilage degeneration score reduction of more than 50%, and the like, which are quantifiable and verifiable, so that a stable, long-acting and curative drug preparation and application scheme are provided for osteoarthritis treatment.

The invention belongs to the technical field of biological detection, and particularly relates to an enzymatic-binding dual-response type albumin fluorescent probe as well as a synthesis method and application thereof. The molecular structural formula of the fluorescent probe is shown in the specification. The fluorescent probe has the advantages of simple synthesis, unique response mechanism, high sensitivity, strong specificity, low cytotoxicity and good cell membrane penetrability, the detection limit is as low as 0.028 [mu] g / mL, the selectivity on albumin is superior to that of other biomolecules (including various proteins, ions, amino acids, carbohydrates and biological metabolites), the cell survival rate at the concentration of 30 [mu] M exceeds 90%, and the fluorescent probe has good application prospects. And the product can be taken by cells in only 10 minutes. The ALB fluorescent probe is especially suitable for intracellular albumin detection and fluorescence imaging analysis.

CRISPR-Cas systems have been engineered for various purposes, such as genomic DNA cleavage, base editing, epigenome editing, and genomic imaging. Although significant developments have been made, there still remains a need for new and useful CRISPR-Cas systems as powerful precise genome targeting tools. The invention disclosed herein comprises CRISPR-Cas based compositions and methods for high integration efficiency and expression efficiency of transgenes together with high post-transfection cell viability in eukaryotic cells.

The invention relates to the technical field of biochemical engineering, and particularly discloses a polyester fiber membrane and a preparation method thereof. The polyester fiber membrane is prepared from a copolymer shown in the formula I through electrostatic spinning. The prepared polyester fiber membrane is uniform in fiber diameter, good in gas permeability and high in relative cell survival rate, and has wide application prospects in the field of biomedicine. I

The application provides an amniotic membrane stem cell and a separation method and a hydrogel 3D culture method thereof, relates to the technical field of cell culture, and comprises the following steps: placing amniotic membrane fragments in a pre-digestion buffer in sequence and incubating for 10-20 min, placing the amniotic membrane fragments in a mixed enzyme solution and performing digestion treatment, filtering, and obtaining a filtrate and residual amniotic membrane fragments; centrifuging the filtrate to obtain a cell precipitate, and performing differential adhesion culture to obtain amniotic membrane epithelial stem cells; adding a culture medium to resuspend the residual amniotic membrane fragments so that the residual amniotic membrane fragments are in contact with a culture container and are in a non-suspended state, performing adhesion culture, and obtaining amniotic membrane mesenchymal stem cells that have climbed out. Compared with the number of cells and the cell survival rate of cells that are subjected to digestion treatment of other mixed enzymes, the number of cells obtained by digestion treatment of the mixed enzyme is significantly increased, and the cell survival rate is higher; compared with a step-by-step digestion strategy, the mixed enzyme digestion one-step method is more simple, and efficient and synchronous separation is achieved. The technical problem of a significant decrease in cell activity caused by the step-by-step digestion separation strategy in the prior art is solved.

The invention relates to the technical field of embryonic development evaluation, and discloses an embryonic chromatin openness and gene expression evaluation method, which comprises the following steps: extracting an embryonic cell sample with a survival rate of more than or equal to 95% through a micromanipulation technology; acquiring chromatin open signal data by adopting an ATAC-seq technology; carrying out genome positioning by utilizing a BWA comparison tool, and constructing a three-dimensional contact matrix through a matrix decomposition algorithm in combination with Hi-C data; determining the gene expression quantity by adopting fluorescent quantitative PCR (Polymerase Chain Reaction); and constructing an XGBoost integrated learning model to fuse chromatin characteristics and gene expression data. By optimizing micromanipulation parameters and an ATAC-seq experimental process, the cell survival rate is increased to 95.2 + / -1.3%, the ATAC-seq library complexity reaches 85.3%, and Hi-C data standardization processing is combined, so that the technical problem of data deviation is solved, and the embryo sample processing efficiency and data reliability are remarkably improved.

The invention provides application of ML324 in preparation of drugs for treating and / or preventing heart diseases. The invention shows that the ML324 can prevent and / or treat heart diseases related to myocardial cell death, obviously inhibit activity decline of myocardial cells induced by a cell death inducer Erastin and H2O2, and improve the cell survival rate; generation of active oxygen in cells is reduced, and death modes such as myocardial cell apoptosis are reduced; the compound can be used for preventing, treating and improving heart function damage, myocardial fibrosis and heart organic damage caused by heart diseases, has a good application prospect in the aspect of developing medicines for resisting the heart diseases, and provides a new medicine research and development direction for preventing, treating and improving the heart diseases.

The application relates to the technical field of new uses of medicines, and particularly discloses application of an N-phenylpiperazine derivative in preparation of a medicine for treating Parkinson's disease, wherein the N-phenylpiperazine derivative is HS. + The N-phenylpiperazine derivative HS provided by the application can significantly improve the survival rate of SH-SY5Y cells induced by MPP + , shows a dose-dependent neuroprotective effect, can effectively improve the motor dysfunction of a model animal, increase the open field movement distance, prolong the time of staying on a rotating rod, and provides a new selection for developing an anti-Parkinson's disease treatment medicine with a neuroprotective effect.

The invention belongs to the technical field of cryoprotectants, and particularly relates to a multifunctional bone tissue cryoprotectant and a preparation method thereof. In the multifunctional bone tissue cryopreservation solution, every 1000 ml of the cryopreservation solution is composed of the following components: 20-60 mmol of NaHCO3, 20-60 mmol of NaHCO3, 20-60 mmol of NaHCO3, 20-60 mmol 0.6 to 1.0 mmol of MgSO4; 1.0 mmol to 1.4 mmol of CaCl2; 60 to 80 mmol of KCl; 60 to 100 mmol of NaCl; 60 to 100 mmol of reduced glutathione; 100 to 140 mmol of TES (tetraethyl orthosilicate); 60 to 100 mL of propylene glycol; 6 to 10 mmol of D-glucose; and the balance of deionized water. The multifunctional bone tissue cryopreservation liquid provided by the invention is low in toxicity, high in cell survival rate, strong in antibacterial ability, long in preservation time and high in degreasing ability, and the cryopreservation effect of bone tissues and bone soft tissues is effectively improved.

The invention discloses a bacteriostatic and anti-inflammatory polypeptide extract and application thereof in a Yao bath. The polypeptide extract is extracted from rhizomes of Coptis chinensis (Coptis chinensis Franch.), and is obtained through extraction with a phosphate buffer solution, ultrafiltration and gel chromatography purification, and the polypeptide is as shown in SEQ ID No: 1. Experiments show that the polypeptide extract has significant bacteriostatic activity on pathogenic bacteria such as escherichia coli, shows a dose-dependent anti-inflammatory effect in an RAW264.7 cell model, can effectively inhibit NO release, and has a cell survival rate of 92% or more and excellent biological safety. The polypeptide extract is applied to a Yao bath preparation, O / W type emulsion is prepared, 10 volunteers try on the Yao bath preparation, the improvement rate on mild skin inflammation symptoms (such as red and swollen and pruritus) reaches 87%, and anaphylactic reaction is avoided. The polypeptide extract is simple in preparation process and high in activity, and has a wide application prospect in the fields of cosmetics and medicines.

The invention belongs to the technical field of bioengineering, and particularly provides application of a JNK inhibitor in preparation of drugs for preventing and / or relieving and / or treating pulmonary tuberculosis aiming at the problem that whether the existing JNK inhibitor SP600125 can inhibit BNIP3-mediated mitochondrial autophagy and further exert the ROS bactericidal effect is unknown. And the JNK inhibitor is an SP600125 JNK inhibitor. The JNK inhibitor is used for inhibiting mitochondrial autophagy in the BCG infected macrophages. The JNK inhibitor reduces the up-regulation of BNIP3 and LC3B caused by BCG infection. It is found through the application that when the concentration of the JNK inhibitor reaches 15 umol / L, remarkable cytotoxicity begins to appear, and compared with a control group, the cell survival rate is remarkably decreased. The SP600125 can be used for inhibiting mitochondrial autophagy and down-regulating the expression of BNIP3 and LC3B. Inhibition of JNK expression can inhibit survival of Mtb in RAW264.7 macrophages, and a new thought and direction are provided for treatment of tuberculosis.

The invention relates to the technical field of cell culture, discloses an integral bubble-eliminating organ chip, and aims to solve the problems of cell necrosis and experiment failure caused by bubbles in a perfusion process of an existing organ chip. The chip comprises a sealing cover plate, a substrate, a bubble trapping mechanism and an attachment mechanism, bubbles in perfusate are efficiently eliminated through the bubble trapping mechanism, the attachment mechanism provides a stable attachment surface for cells, bidirectional circulation of the perfusate is achieved, and a guide-in opening and a guide-out opening do not need to be distinguished. The bubble elimination rate reaches 99.9%, the cell survival rate is improved to 95% or above, the repeatability of experimental data is improved by 80%, expensive peripherals are not needed, the use threshold of an organ chip platform is lowered, and the method is suitable for long-term and high-throughput cell culture and organ function simulation experiments.

The invention provides a method for evaluating the intestinal toxicity of nano polypropylene plastic, which comprises the following steps of: 1, exposing macrophages in a culture medium containing polypropylene nano particles with different concentrations and / or different particle sizes for culturing, and detecting any one or more of cell survival rate, glucose consumption level, lactic acid production level and glycolysis level; the method 2 comprises the following steps: exposing an enteritis model mouse in the polypropylene nano-plastic, recording the fecal state, hematochezia degree and weight during administration, and / or calculating a disease activity index, and / or detecting the pathological state of the intestinal tract of the mouse. The invention focuses on analyzing the influence of the polypropylene nano-plastic on glycolysis of macrophages, reveals the potential effect of the polypropylene nano-plastic on enteritis progression, and provides theoretical basis and technical support for prevention and treatment of nano-plastic related diseases.

The invention belongs to the technical field of biology, and discloses a serum-free culture medium and application thereof in obtaining nerve cells through embryo fibroblast transdifferentiation. The serum-free culture medium consists of a basic culture medium, a protein additive and cell culture auxiliary components, the protein additive consists of transferrin, a leukemia inhibition factor and a basic fibroblast growth factor; the final concentration of the transferrin is 5 ng / mL-30 ng / mL, the final concentration of the leukemia inhibition factor is 50 ng / mL-150 ng / mL, and the final concentration of the basic fibroblast growth factor is 10 ng / mL-25 ng / mL. The serum-free culture medium can promote the conversion speed of transdifferentiation of fibroblasts into nerve cells, can significantly improve the transdifferentiation efficiency, and can improve the cell survival rate of transdifferentiation of mouse embryo fibroblasts into nerve cells.