Improved mesenchymal stem cell protective solution and application thereof

A technology of mesenchymal stem cells and protective solution, which is applied in the field of improved mesenchymal stem cell protective solution, can solve the problems of cell chemical damage and achieve the effects of reducing cell activity loss, improving stress resistance, and reducing accumulation

Active Publication Date: 2018-06-01
北京唐颐惠康生物医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, vitrification requires the use of cytotoxic dime

Method used

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  • Improved mesenchymal stem cell protective solution and application thereof
  • Improved mesenchymal stem cell protective solution and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] An improved mesenchymal stem cell protection solution, comprising the following ingredients in parts by weight:

[0035] 20 parts of osmotic protectant, 20 parts of protein composition, 0.1 part of cell physiological activity inhibitor and 5 parts of amifostine;

[0036] The osmoprotectant includes 12.5 parts of dimethyl sulfoxide, 5 parts of raffinose and 2.5 parts of proline;

[0037] The protein composition includes 2.5 parts of antifreeze protein, 5 parts of γ-globulin and 12.5 parts of plant-derived recombinant human serum albumin;

[0038] The cell physiological activity inhibitor includes 0.05 part of octreotide and 0.05 part of tunicamycin.

Embodiment 2

[0040] An improved mesenchymal stem cell protection solution, comprising the following ingredients in parts by weight:

[0041] 25 parts of osmotic protectant, 15 parts of protein composition, 0.2 part of cell physiological activity inhibitor and 2 parts of amifostine;

[0042] The osmoprotectant includes 12.5 parts of dimethyl sulfoxide, 5 parts of raffinose and 7.5 parts of proline;

[0043] The protein composition includes 2.5 parts of antifreeze protein, 5 parts of gamma-globulin and 7.5 parts of plant-derived recombinant human serum albumin;

[0044] The cell physiological activity inhibitor includes 0.1 part of octreotide and 0.1 part of tunicamycin.

Embodiment 3

[0046] An improved mesenchymal stem cell protection solution, comprising the ingredients described in Example 1, and also including 2 parts by weight of sodium lactated Ringer.

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Abstract

The invention provides an improved mesenchymal stem cell protective solution. The concentration of electrolytes in an unfrozen water solution inside and outside cells is balanced through a permeatingprotective agent, so that cell damage due to excessive dehydration of cells in the outside high-permeability environment is avoided; added protein composition can supply cells with required nutrients,and accordingly, the cell survival rate is increased; a physiological activity inhibitor of cells can reversibly inhibit physiological activities in the cells, and the cryopreservation effect is improved on the premise that the cell activity is not affected; amifostine is a broad-spectrum cell protective agent and a radiation protective agent and can improve the tolerance of cells to the outsideadverse environment, reduce accumulation of toxic substances in cells and improve the proliferative activity of stem cells. Through combined use of components, the stress resistance of mesenchymal stem cells can be effectively improved, cell activity loss caused in the cryopreservation process can be reduced, and the cell survival rate and the thawing rate after cryopreservation are increased.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation and resuscitation of human tissue, and in particular relates to an improved mesenchymal stem cell protection solution and its application. Background technique [0002] Mesenchymal stem cells are important members of the stem cell family. They are primitive cells with self-replication and multi-directional differentiation potential. They are the origin cells of the body and the primitive cells that form various tissues and organs of the human body. Under certain conditions, it can differentiate into a variety of functional cells or tissues and organs, which are called "universal cells" in the medical field, and are suitable as seed cells for the repair of tissue and organ damage caused by aging and disease. Therefore, storing mesenchymal stem cells can provide important medical materials. The traditional programmed cryopreservation tends to cause mechanical damage to the cells because it...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 曹毓琳刘俊江时兆田白志惠
Owner 北京唐颐惠康生物医学技术有限公司
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