36results about How to "Improve cryopreservation effect" patented technology

The invention discloses new application of tetrahydropyridines, namely the application of the tetrahydropyridines to the cryopreservation of cells, tissues and organs. The effective concentration is 0.1g/L to 100g/L; and the preferred concentration is 0.5g/L to 20g/L. During use, the tetrahydropyridines can be used separately or can be jointly used with other chemical reagents for the cryopreservation of cells, tissues and organs. Through experiments, the inventor of the application verifies that: the tetrahydropyridines can help the cells in resisting the refrigeration conditions and improve the protective effect on the cryopreservation of cells, tissues and organs. If the tetrahydropyridines is jointly used with dimethyl sulfoxide, the tetrahydropyridines can reduce the dosage of the dimethyl sulfoxide, reduce the toxicity of the dimethyl sulfoxide on the cells, and improve the cryopreservation effect.

The invention provides a method for rapidly cryopreserving and resuscitating endometrial stem cells. The method is capable of reducing the probability of cell damage and apoptosis and improving the survival rate. According to the method, cryopreservation fluid or resuscitation fluid is added into the cells to perform short-term low-frequency ultrasonic treatment, so that mutually adhesive cells can produce slight loosening and gaps on premise of not damaging the cell structure, and the fluid can be dispersed into droplets and conveniently infiltrates into the cells; cell adhesion molecules arecapable of promoting mutual adhesion of the cells, so that individual cells are further reduced, and the cell survival rate is improved; and a cell activity inhibitor is capable of performing reversible inhibition on some physiological activities in the cells, and the cryopreservation effect is improved on premise of not influencing the cell activity. According to the means, physical and chemicaldamage to the cells in the cryopreservation and resuscitation process can be reduced, so that the cell survival rate and cryopreservation and resuscitation rate of the cells are improved.

The invention provides an umbilical cord tissue freeze preservation and unfreezing method. According to the umbilical cord tissue freeze preservation and unfreezing method, cell aggregate freeze preservation is adopted instead of single cell freeze preservation, so that cell damage apoptosis rate is reduced, and survival rate is increased; cell adhesion molecules are added to promote bonding of cells, reduce the amount of single cells, and increase cell survival rate; ultrasonic treatment is adopted so as to ensure full penetration of a refrigeration protective solution and an unfreezing protective solution into intercellular spaces, solutions are dispersed into small liquid drops, and the permeability is improved; a cell activity inhibitor is adopted for reversible inhibition of physiological activities in cells, and improving of freeze preservation effect without influencing cell activity. The umbilical cord tissue freeze preservation and unfreezing method is capable of reducing physical and chemical damages on cells in freezing and unfreezing process, and increasing cell survival rate and unfreezing rate, so it is convenient for subsequent using.

The invention discloses a boar seman cryoprotectant which is prepared from the following substances: cryopreservation basic liquid, fresh egg yolk, glycerinum, astaxanthin and a radix rehmanniae preparata extract. The boar seman cryoprotectant is subjected to special matching treatment; under the common action of all the components, the finally prepared cryoprotectant obviously enhances the boar seman cryopreservation effect, and increases the boar seman viability, acrosomal integrity and film integrity rate; the fertilization vitality and the use effect of boar semans are guaranteed to the maximum extent.

The invention relates to the technical field of cryopreservation solutions, in particular to a cell cryopreservation solution, a method for cryopreserving hematopoietic stem cells by using the cell cryopreservation solution and a stem cell preparation. The invention discloses a cell cryopreservation solution which is composed of DMSO, a human serum albumin injection and a plasmon A injection. Thecell cryopreservation liquid is simple and safe in components, the components are matched with one another, a synergistic effect is achieved, the cell cryopreservation effect can be remarkably improved, the survival rate of cryopreserved hematopoietic stem cells after recovery is high, the cell stability is good, and treatment of diseases is facilitated.

The invention relates to a swine semen freezing protecting agent and a cryopreserving method for swine semen by using the same. The swine semen freezing protecting agent is prepared from the followingcomponents in parts by weight: 2.5 parts of glucose, 2 parts of citric acid, 3 parts of coconut oil monoethanolamide, 2.5 parts of hyaluronic acid, 1.5 parts of skimmed milk, 1.2 parts of trehalose,0.07 part of penicillin sodium, 0.2 part of streptomycin sulfate, 95 parts of double distilled water, 23 parts of fresh yolk, 3.5 parts of glycerin, 1.5 parts of astaxanthin and 5 parts of extract ofradix rehmanniae praeparata. The cryopreserving method comprises the following steps of firstly, collecting the swine semen, centrifuging, and filtering, so as to obtain the swine semen A; pouring onepart of swine semen A into an experiment bottle, and adding two parts of freezing protecting agent to thin; finally, adding into liquid nitrogen, and cryopreserving. The swine semen freezing protecting agent has the advantages that the freezing protecting agent is reasonably compounded, so that the cryopreserving effect of the swine semen is improved under the function of the components; the survival rate, acrosome integrity and plasmalemma integrity of the swine semen are improved; the insemination activity and use effect of the swine semen are guaranteed.

The invention relates to macrophage cryopreservation liquid and a macrophage cryopreservation method. The macrophage cryopreservation liquid is prepared from the following components: glucan, bovine serum albumin, sorbitol, low molecular dextran, chitosan and RPMI 1640. The macrophage cryopreservation method utilizes the macrophage cryopreservation liquid to cryopreserve macrophages. According to the macrophage cryopreservation liquid and the macrophage cryopreservation method, the cryopreservation effect of the macrophages is improved, the survival rate is relatively high after recovery, the growth state of cells is good and the generation time is relatively long; the macrophage cryopreservation liquid does not utilize FBS (Fasting Blood Sugar) so that the safety of clinical transfusion is greatly improved; and the content of DMSO (Dimethylsulfoxide) is reduced and the toxin risks to human bodies are reduced.

The invention discloses a cryopreservation method for pinus elliottii embryogenic calluses resistant to pine needle spot brown spot. According to the method provided by the invention, proper pre-culture and a mixed cryoprotectant facilitate improving the cryopreservation effect, after thawing is performed at 32 DEG C, the calluses frozen for 90 days can be regenerated after 1 month, the regeneration rate of the embryogenic calluses is up to 100%, and the regenerated calluses grow well and still retain the ability to differentiate mature somatic embryos; and the method preliminarily establishesa cryopreservation system for the pinus elliottii embryogenic calluses resistant to the pine needle brown spot, and provides a technical reference for embryogenic long-term maintenance of the resistant pinus elliottii embryogenic calluses.

The invention discloses a cell seedling temperature cryopreservation method and a device, and belongs to the technical field of cell cryopreservation, the method comprises the following steps: S1, cooling a cryopreservation tube loaded with cells to be cryopreserved through a refrigeration system according to a preset cooling rate, and detecting the temperature of the cryopreservation tube in the cooling process; S2, judging whether the temperature of the cryopreservation tube reaches a preset seedling temperature or not, if yes, executing S3, and if not, executing S1 continuesly; S3, adopting the refrigeration system to conduct heat preservation on the cryopreservation tube at the preset seedling temperature; S4, applying ultrasonic waves to the cryopreservation tube through an ultrasonic vibrator so as to carry out seedling temperature operation, and lasting the operation for preset threshold time; S5, judging whether seedling temperature operation of the cryopreservation tube succeeds or not by analyzing the temperature of the cryopreservation tube before and after the seedling temperature operation, if yes, executing S6, and if not, continuing to execute S4; and S6, cooling the cryopreservation tube continuesly through the refrigerating system, stopping cooling after the cryopreservation tube is cooled to the preset cryopreservation temperature, and carrying out heat preservation. According to the method, the cell cryopreservation effect is improved by adding the step of judging whether seedling temperature operation succeeds or not.

The invention relates to the field of biotechnology, in particular to a cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof. The CBMC cryopreservation solution is prepared from dimethyl sulfoxide (DMSO) combined hydroxyethyl starch (HES), propylene glycol, human albumin and a buffer solution. Compared with a conventional formula, the CBMC cryopreservation solutioncan avoid contamination of animal-derived pathogens, does not take a cell culture medium as a component of a cryoprotectant, and ensures good cell cryopreservation effect. After cryopreservation for 2months, the average cell viability of CBMC cryopreserved with the cryopreservation solution is 97.47% to 98.43%. The recovered cell can be directly used for clinical transplantation, and the CBMC cryopreservation solution is of practical value in clinical application of cell therapy.

The invention provides a method for improving an encapsulation-dehydration ultralow-temperature preservation effect of early pear stem tips. The early pear stem tips are treated in sequence by utilizing four types of culture media containing carbon nanotubes and graphene quantum dots, and the survival rate of the frozen stem tips can be remarkably improved. Compared with the prior art, the methodhas the advantages that the encapsulation-dehydration ultralow-temperature preservation effect of the early pear stem tips is good, the survival rate is high and the stem tips are easy to regenerate.

The invention provides a method for improving a droplet-vitrification ultralow-temperature preservation effect of early pear stem tips. The early pear stem tips are treated in sequence by utilizing six types of culture media containing carbon nanotubes and graphene quantum dots, and the survival rate of the frozen stem tips can be remarkably improved. Compared with the prior art, the method has the advantages that the droplet-vitrification ultralow-temperature preservation effect of the early pear stem tips is good, the survival rate is high and the stem tips are easy to regenerate.