217 results about "Poloxamer" patented technology

The invention relates to the technical field of medical biopolymer materials, in particular to a two-component gynecological gel spray and a using method thereof.The two-component gynecological gel spray comprises a first component and a second component, and the first component at least comprises formylated multi-arm polyethylene glycol, formylated poloxamer and a solvent; the second component at least comprises a multi-amino polymer solution, the bi-component solution forms a film in the vagina and is cured in situ, and the vaginal gel has the characteristics of strong adhesion and strong corrosion resistance, can act on the vagina for a long time to realize long-acting treatment, and is more convenient to use and better in effect.

The invention relates to a pharmaceutical composition, which is prepared from adapalene, benzoyl peroxide, a traditional Chinese medicine extract, an acrylamide and acryloyl dimethyl sodium taurate copolymer, a mixture of isohexadecane and polysorbate 80, docusate sodium, EDTA (Ethylene Diamine Tetraacetic Acid) disodium, glycerol, poloxamer 124, propylene glycol and water, the traditional Chinese medicine extract comprises moutan bark, salvia miltiorrhiza, radix rehmanniae recen, radix sophorae flavescentis, coptis chinensis, rheum officinale, scutellaria baicalensis, menthol and borneol. According to the pharmaceutical composition disclosed by the invention, the synergistic antibacterial and anti-keratosis curative effects of adapalene and benzoyl peroxide can be maintained, and meanwhile, the occurrence rate of local skin dryness, erythema, desquamation, burning heat sensation and contact dermatitis can be remarkably reduced.

The application discloses a kind of by polydopamine mesoporous nanoparticles crosslinked sulfhydrylated hyaluronic acid hydrogel and its preparation method and application, with dopamine and 4-formylphenylboronic acid as monomer, with trimethylbenzene as and poloxamer as template agent, in weak alkaline (pH=8.4), oxygen exists in the environment, by the oxidation self-polymerization reaction between monomer Preparation of polydopamine nanoparticles with mesoporous structure.Then the water dispersion of polydopamine nanoparticles is mixed with sulfhydrylated hyaluronic acid solution according to certain proportion, and the hydrogel dressing with injectable property is prepared.The hydrogel is expected to be used as skin wound dressing for the treatment of diabetic wounds.

The invention discloses a blue copper peptide composition for repairing scars and a preparation method of the blue copper peptide composition, and belongs to the technical field of preparation of biological medicine preparations. The composition is prepared from freeze-dried powder of copper blue peptide, polyquaternium-73, asiaticoside, nicotinamide, polydimethylsiloxane, betaine-propylene glycol ionic liquid and a gel matrix. According to the invention, the blue copper peptide is coated in a permeation enhancing system constructed by the betaine-propylene glycol ionic liquid and the HEPES, so that the enzymolysis loss of the blue copper peptide is reduced, and the biological activity of the blue copper peptide is maintained; a temperature-sensitive gel matrix is constructed by carboxyl esterified modified sodium alginate and poloxamer, the carboxyl esterified modified sodium alginate is prepared by grafting n-butyl alcohol to a hydrophilic main chain of sodium alginate through a Fischer esterification method, the gel matrix is in a liquid state at normal temperature, after the gel matrix is smeared on a scar part, the gel matrix is converted into semi-solid gel under the influence of body temperature, and slow and continuous release of blue copper peptide can be achieved. The traditional Chinese medicine composition has the effect of repairing scars and is suitable for chronic scars needing long-term nursing.

Disclosed is a composition for treating a joint disease or a connective tissue disease, a composition for cartilage regeneration, or a composition for treating an inflammatory disease, each of the compositions containing dextran, poloxamer or a mixture thereof. The composition stays in the joint or connective tissue for a long time due to the shock-absorbing effect, coating effect or anti-inflammation effect, relieves the shock, covers a damaged portion in a specific manner thereto, or reduces inflammation of an adhered portion. Thus, the composition may be useful for the treatment of the joint disease, the connective tissue disease, or for the cartilage regeneration.

Improved compositions and processes for preparing mRNA-loaded lipid nanoparticles (mRNA-LNPs) are provided.SOLUTION: (i) mixing a mRNA solution with a lipid solution in the presence of 0.05% to less than 3% of a poloxamer prior to removal, wherein the lipid solution comprises one or more cationic lipids, one or more non-cationic lipids, and less than 0.5% of one or more PEG-modified lipids or PEG, and (ii) removing the poloxamer, wherein upon removal less than 0.05% of the poloxamer remains.SELECTED DRAWING: Figure 2

This application belongs to the field of biomedicine, specifically relating to a method for preparing an antitumor composition for pets, aiming to solve the technical problems of low bioavailability, poor stability, and poor drug administration compliance of poorly soluble active ingredients in antitumor drugs for pets. The method includes: a raw material pretreatment step, in which curcumin, resveratrol, quercetin, green tea polyphenols, and astragalus polysaccharides are pulverized and sieved separately; an organic phase preparation step, in which egg yolk lecithin and solid lipids are dissolved in an ethanol-acetone mixed solvent, and curcumin and resveratrol are added and dissolved by stirring in a water bath; an aqueous phase preparation step, in which poloxamer F68 is dissolved in purified water and dissolved in a water bath; a high-pressure homogenization step, in which a nanoemulsion containing a solid lipid core is prepared; a moderately polar component encapsulation step, in which quercetin and green tea polyphenols are ultrasonically dispersed and then added dropwise to the nanoemulsion; a freeze-drying step, in which a nano-lyophilized powder is obtained; and an outer hydrophilic component coating step, in which astragalus polysaccharides are sprayed or adsorbed onto the surface of the freeze-dried powder. The composition obtained by this method is a core-shell multilayer nanoparticle with a core consisting of curcumin and resveratrol encapsulated by solid lipids, a middle shell adsorbing quercetin and green tea polyphenols, and an outer shell coated with astragalus polysaccharides. This application utilizes the above-mentioned layered encapsulation technology to achieve stable co-encapsulation of multiple antitumor active ingredients, improve the bioavailability of poorly soluble components, optimize drug stability, and enhance antitumor effects through multi-target synergistic effects. Simultaneously, it endows the composition with immunomodulatory functions and sustained-release properties. The composition can be formulated into a nano-lyophilized powder form, making it easy to add to pet food or formulate into a paste for administration, improving pet drug compliance. Figure 2 is a schematic diagram of the three-layer core-shell nanoparticle structure of the composition of this invention.

The invention discloses a preparation method of an intestinal canal filling temperature-sensitive gel injection for gastrointestinal anastomosis guided by an ultrasonic endoscope, and belongs to the technical field of regenerative medicine. According to the specific method, poloxamer, chitosan, polycarbophil, polyvinylpyrrolidone, iohexol and methylene blue are utilized to construct the novel intestinal canal filling temperature-sensitive gel injection. The injection can be injected into the anastomotic intestinal segment of the small intestine by using a conventional nasobiliary drainage tube which is clinically used at present, and the injection becomes viscous immediately under the action of the pH value of the small intestine after injection, so that the injection is inhibited from spreading all around. Meanwhile, the injection is quickly converted into high-strength hydrogel under the action of body temperature, and the hydrogel is adhered to small intestine mucosa to inhibit gel sliding. Therefore, the injection can form a section of gel on a target small intestine section, local immobilization filling and supporting of the target small intestine section are achieved, development can be achieved, the operation difficulty of intestinal tube puncture and stent placement in gastrointestinal anastomosis guided by an ultrasonic endoscope can be greatly reduced, the operation efficiency and the success rate are improved, and the operation cost is reduced. The dosage is less than that of normal saline which is conventionally used clinically, and the effect is better. Moreover, the gel temperature can be reduced by injecting low-temperature normal saline after the operation, so that the gel is thoroughly dissolved into a solution and discharged out of the body, and the phenomenon that the gel remains in the intestinal canal after the operation to cause obstruction is avoided. Therefore, the novel intestinal canal filling temperature-sensitive gel injection has very important clinical practical application value for gastrointestinal anastomosis guided by an ultrasonic endoscope.

The invention discloses an alfacalcidol emulsion and a preparation method thereof, the pH value of the alfacalcidol emulsion is 6.5-8.0, and each 1000 ml of the alfacalcidol emulsion comprises 1-30 g of alfacalcidol, 100-300 g of oil for injection, 6-20 g of a phospholipid emulsifier, 0-20 g of poloxamer 188, 20-30 g of an osmotic pressure regulator and a proper amount of water. The preparation method of the drug-loaded fat emulsion injection comprises the following steps: preparing an oil phase; preparing a water phase; preparing a primary emulsion; performing high-pressure homogenization; adjusting the pH value; and sterilizing and storing. The alfacalcidol serves as an oil-soluble substance, can be dissolved in small oil drops (emulsion particles) of the emulsion or on an interfacial film, and can keep stable physicochemical properties and enhance the slow release performance in the storage process under certain conditions, so that the drug effect time is prolonged.

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed.According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells / mL or more and 400×106 cells / mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

This application provides a premix, kit, and application of a premix for preparing an embolic agent. The premix uses a poloxamer to sodium alginate mass ratio of 9:1 to 20:1, which enables the formation of an injectable solution while maintaining good gel properties, and offers ease of preparation and use.

This invention provides an injectable cefminox sodium preparation and its preparation method, belonging to the field of pharmaceutical preparation technology. The preparation, by weight, comprises: 1 part of refined cefminox sodium, 0.5 parts of sodium lactate Ringer's solution, 0.2 parts of vitamin B12 injection, 0.1-0.3 parts of a modified composite antioxidant, and 3 parts of physiological saline. The preparation method of the modified composite antioxidant is as follows: S1, add 1 part by weight of poloxamer to 20 parts by weight of distilled water and stir for 6-8 minutes to obtain a solution; S2, after passing 1 part by weight of natural vitamin C through a 100-mesh sieve, mix it into the solution obtained in S1, and sonicate for 10-15 minutes to obtain a vitamin C solution; S3, mix 0.5 parts by weight of vitamin D into the vitamin C solution obtained in S2, and sonicate for 15-20 minutes to obtain the modified composite antioxidant. The formulation of this invention exhibits excellent stability and stable content of cefminox sodium when exposed to environments of 35℃±2℃, 65%±5%RH and natural light, ensuring the safety and reliability of medication.

The present application relates to the technical field of enzyme engineering, and in particular to a kind of complex enzyme microcapsule preparation and its preparation method and application.The above-mentioned complex enzyme microcapsule preparation for ruminant feed, raw materials include by weight ratio: complex enzyme 1-2 parts, complex starch carrier 6-20 parts, poloxamer 188 0.1-1 part, sodium alginate 1-2 parts, plant-derived ingredients 1-2 parts, complex trace elements 0.1-0.3 parts, 0.1-0.2 mol / L calcium chloride solution 100-200 parts;The raw materials of complex starch carrier include by weight ratio: starch 5-15 parts, gelatin 1-5 parts, amylase 0.01-0.1 parts, zinc sulfate heptahydrate 0.01-0.1 parts.The present application has high thermal stability and anti-interference ability, remains stable in rumen environment, can effectively improve the activity of true stomach enzyme, improve feed digestibility, and improve the production performance of ruminant.

The present invention relates to a composition that is used to primarily disrupt and remove biofilm from surfaces. This is achieved using a composition comprising a bacteriophage capable of lysing bacteria selected from one or more of Pseudomonas aeruginosa, Acinetobacter baumanii, Staphylococcus aureus, and E.coli; and a Bacillus bacterial spore in the presence of a specific surfactant of the poloxamer type.

The invention relates to the technical field of medical materials, and particularly discloses a heparinized polycaprolactone coating as well as a preparation method and application thereof. The preparation method of the heparinized polycaprolactone coating comprises the following steps: adding poloxamer 188 into an MES buffer solution, adding heparin, uniformly stirring, then adding EDC and NHS, and stirring for 40-120 minutes to prepare an activated heparin solution; and dropwise adding the polycaprolactone solution into the activated heparin solution, reacting at room temperature for 90-180 minutes, then dissolving the conjugate into chloroform through rotary evaporation, washing with water, drying with anhydrous MgSO4, precipitating with methanol, filtering the precipitate, and drying in vacuum at 30-40 DEG C to obtain the polycaprolactone heparin. According to the prepared heparinized polycaprolactone coating, local release of heparin is remarkably reduced, and the continuous anticoagulation effect is achieved; and the anticoagulant activity of heparin is reserved, and the coating with good stability is obtained.

The reduction in the sol-gel temperature of aqueous poloxamer surfactant compositions by the addition of hydrophobic vicinal diols is provided. Lowering of the sol-gel temperature and the gelling efficiency of water-soluble poloxamer block copolymers of polyethylene oxide-b- polypropylene oxide-b-polyethylene oxide has been markedly improved by the addition of small amounts of at least one hydrophobic vicinal diol, such as monoalkyl glycols, monoalkyl glycerols, or monoacyl glycerols. The decrease in the sol-gel temperature facilitates gel formation, and such gels exhibit greater residence time on a surface, particularly those with biological properties.

The invention relates to the technical field of catalyst materials, in particular to a method for synthesizing a dimethyl carbonate catalyst through a hydrothermal method and application. Comprising the following steps: A, preparing poloxamer into a solution, adding HAc and NaClO4, and uniformly mixing to obtain a mixture; b, (NH4) 2Ce (NO3) 6 and H2BDC are added into the mixture in the step A to be stirred, mixed and reacted, the catalyst is obtained through centrifugal separation, washing, soaking, vacuum drying and calcination, crystal grains of the catalyst are reduced, and the specific surface area, the number of acid-base sites and the oxygen vacancy concentration are all greatly improved.

The invention relates to the technical field of oral care materials, and particularly discloses macromolecular hydrogel-based false tooth safety paste and a preparation method thereof. The macromolecular hydrogel-based denture safety paste is prepared from the following components in parts by weight: 10 to 20 parts of white oil, 20 to 40 parts of vaseline, 40 to 60 parts of polydopamine modified polyvinyl alcohol-based hydrogel, 20 to 40 parts of sodium carboxymethyl cellulose, 20 to 40 parts of methyl vinyl ether-maleic anhydride copolymer calcium sodium salt, 0.1 to 1 part of silicon dioxide, 0.1 to 1 part of 93 to 98 percent ethanol, 0.05 to 1 part of preservative, 0.1 to 2 parts of essence and 0.001 to 0.01 part of pigment. The polydopamine modified polyvinyl alcohol-based hydrogel is prepared from polyvinyl alcohol, dopamine hydrochloride, an activating agent, a coupling agent and poloxamer. According to the technical scheme provided by the invention, the prepared false tooth fixing paste has excellent properties of good bonding effect and strong durability.

Disclosed is a method for the enhancement of the transduction of a target cells by a viral vector using a cationic block-copolymer introduced as an additive alone or formulated with nanoparticles. The method includes a step of contacting a target cells with viruses and a cationic block co-polymer. The structure of this additive incorporates both hydrophilic and hydrophobic regions which represents different areas in the backbone of the polymer. This polymeric construction is ended by cationic chemical functions which contribute to further enhance the viral transduction. Also disclosed are new cationic poloxamers that can be used in the disclosed method. Furthermore, another embodiment is the colloidal stabilization of iron-based nanoparticles using these polymers and their use in increasing transduction efficiency.

The application discloses an exosome freeze-drying composition and a preparation method thereof, relates to the field of pharmaceutical preparations, and comprises the following components in parts by mass: 1E9 particles exosome, 1-2 parts of PEG2000 modified beta-sitosterol, 2-4 parts of cholesterol, 20-30 parts of HSA, 30-40 parts of trehalose and 0.5-1.5 parts of poloxamer 188. The preparation method comprises the following steps: step one, preparation of a lipid film; step two, hydration of the liposome; step three, fusion with the exosome; step four, preparation of a pre-freeze-drying suspension; and step five, freeze-drying and sub-packaging. The formula and the preparation method can effectively protect the structural integrity of the exosome in the freeze-drying process and improve the stability of the exosome.

The invention discloses salicylic acid sustained-release microspheres prepared by a cyclodextrin inclusion technology and a preparation method of the salicylic acid sustained-release microspheres, and belongs to the technical field of pharmaceutical preparations. The method aims to solve the problems of poor solubility, low drug loading capacity and lack of sustained-release capability of traditional cyclodextrin inclusion salicylic acid. The core of the method is that two brand new functional compounds, namely quaternary ammonium salt hydroxypropyl beta cyclodextrin and nicotinamide poloxamer covalent conjugates, are firstly prepared; when the microspheres are prepared, the two compounds are dissolved in a mixed solvent of ethanol and water, then salicylic acid and nicotinamide are added, a supramolecular inclusion solution is formed through stirring, and finally a microsphere product is obtained through spray drying and screening. Wherein the affinity with the skin is enhanced by the cationic cyclodextrin derivative, the nicotinamide conjugate and salicylic acid form a stable compound through intermolecular interaction, and efficient inclusion and loading of salicylic acid are realized through synergism of the cationic cyclodextrin derivative and the nicotinamide conjugate. The dissolvability, the solution transparency and the storage stability of salicylic acid are remarkably improved, the microsphere wrapping amount is high, a good slow-release effect is achieved, and the salicylic acid microsphere is suitable for the field of skin external preparations.

The invention provides a long-acting brexpiprazole in-situ gel injection and a preparation method thereof. The long-acting brexpiprazole in-situ gel injection is an injectable sustained-release preparation prepared by taking a polylactic acid-glycolic acid copolymer (PLGA) and poloxamer as gel matrixes, dissolving brexpiprazole in an amphiphilic solvent and adding an additive. The long-acting brexpiprazole in-situ gel injection prepared by the invention has good mechanical properties, and the drug release time can be maintained for 1 month. The preparation is simple in preparation process, low in equipment requirement and suitable for industrial production.

The present application belongs to the field of medical material surface functionalization, and particularly relates to a kind of nano drug-loaded hydrogel catheter coating and its preparation method and application.The present application utilizes PLGA to load antibiotic amikacin sulfate AMK or antibacterial peptide FK13-a1, synthesizes nanoparticle NP-AM and nanoparticle NP-FK, prepares NP-AM / FK@OMV nanoparticle complex with EcN OMV as carrier, then adds poloxamer-hyaluronic acid composite hydrogel to obtain NP-AM / FK@OMV-P / H nano drug-loaded hydrogel, and forms coating after coating and drying.The nano drug-loaded hydrogel coating provided by the present application has the characteristics of enzyme response, antibacterial, prevention of biofilm formation and low toxicity, has great application potential in inhibiting medical catheter surface biofilm, and has important significance for preventing and treating CAUTI, prolonging the use time of indwelling catheter, and reducing the discomfort of patients caused by frequent replacement of catheter.

The invention is applicable to the technical field of biopharmacy process, and provides a freeze-drying method for improving the bioactivity of recombinant mussel mucin, which comprises the steps of pretreatment, pre-freezing, primary drying and secondary drying. In the pretreatment step, a recombinant mussel mucoprotein solution with the pH value of 3.5-5.5 is mixed with a composite protective agent composed of trehalose, L-arginine and poloxamer according to a specific proportion; in the pre-freezing process, slow-fast-slow sectional cooling is adopted; drying adopts a vacuum drying mode combining gradient heating and gradient vacuum degree; according to the invention, creative coupling of the protective agent and the process is realized, the retention rate of the prepared product DOPA is more than 90%, the residual moisture is lower than 1.5%, the redissolution time is shorter than 30 seconds, the microstructure is excellent, and the long-term storage stability is good.

The invention relates to a lipidosome fish oil preparation for pets and a preparation method and application thereof, and belongs to the technical field of pet nutrition and veterinary medicine. The liposome fish oil preparation for pets provided by the invention comprises 10-50 parts of fish oil, 20-60 parts of hydrogenated lecithin, 4-8 parts of succinic acid modified hydroxypropyl methyl cellulose, 4-8 parts of pectin, 1-3 parts of poloxamer 188, 0.5-1 part of chitosan and 0.1-5 parts of an antioxidant, through a liposome encapsulation technology, EPA and DHA in the fish oil are effectively protected, the bioavailability and stability of the fish oil are remarkably improved, the palatability is improved, and the preparation is suitable for pets. Efficient absorption and targeted delivery of EPA and DHA can be achieved, improvement is conducted according to the physiological characteristics and digestion characteristics of pets, and compared with an existing pet fish oil supplement, the pet fish oil supplement has obvious market competitiveness.

The invention belongs to the technical field of biology, and relates to an exosome active cryoprotectant and a preparation method thereof. The exosome activity freezing protection liquid is prepared from trehalose, proline, hydroxypropyl methyl cellulose, poloxamer 188, polyvinyl alcohol, propylene glycol and a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution. According to the exosome preserving fluid and the exosome preserving method provided by the invention, the stability of the exosome can be effectively improved, the preservation time of the exosome is prolonged, and meanwhile, the biological activity of the exosome is maintained.

This invention belongs to the field of pharmaceutical formulation technology and discloses a baloxavir derivative tablet, its preparation method, and its application. The tablet comprises a surface-acidified passivated magnesium aluminum silicate carrier, baloxavir ester, poloxamer 188, microcrystalline cellulose, and magnesium stearate. During preparation, anhydrous citric acid is used to neutralize the basic active sites of pure magnesium aluminum silicate to construct micro-acidic channels. Subsequently, the carrier, baloxavir ester, and poloxamer 188 are mixed and heated, causing the polymer to melt, encapsulate the drug, and penetrate into the mesopores of the carrier. Finally, forced quenching treatment is performed to confine the drug in an amorphous or nanocrystalline state within the channels. This invention blocks the alkaline-catalyzed degradation pathway of baloxavir ester, maintains the high free energy state of the drug through physical spatial confinement, improves the in vitro dissolution rate, and enhances the flowability of the compressed powder.

The invention relates to nucleophilic aromatic substitution reactions done in an aqueous micellar solvent system, wherein the micelles are formed by specific alkoxylated alcohols.

The invention relates to the technical field of medicinal preparations, and particularly discloses a high-stability montmorillonite suspension and a preparation method thereof. The suspension comprises montmorillonite, poloxamer 188 as a surface modifier, xanthan gum as a suspending aid, glycerin as a wetting agent, potassium sorbate as a bacteriostatic agent, a pH regulator and purified water. The preparation method comprises the following steps: dispersing montmorillonite, stirring and modifying with a surface modifier at 40-50 DEG C, mixing with a suspending aid solution and a wetting agent, carrying out high-speed shearing homogenization and colloid mill circulation treatment, finally adjusting the pH value to 5.5-6.5, filtering and filling to form a stable suspension with a microsphere-like structure. According to the invention, the long-term stability of the montmorillonite suspension is obviously improved, the settling volume ratio is high, the redispersibility is good, and the montmorillonite suspension is suitable for industrial production.