106 results about "Melittin" patented technology

The invention discloses a polypeptide composition for repairing, whitening and removing wrinkles. The polypeptide composition for repairing, whitening and removing the wrinkles is prepared from the following components is percentage by weight: a repairing polypeptide composition, a collagen supplementing polypeptide composition, a wrinkle removing polypeptide composition, a skin-brightening polypeptide composition, a small molecular active substance, a skin-care activating agent, a pH (Potential of Hydrogen) regulator, a plurality of auxiliary agents and the balance of water, wherein the repairing polypeptide composition comprises palmitoyl tetrapeptide-7 and tripeptide-1-copper; the collagen supplementing polypeptide composition comprises palmitoyl tripeptide-1, palmitoyl tripeptide-5 andpalmitoyl pentapeptide-4; the wrinkle removing polypeptide composition comprises acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and melittin; the skin-brightening polypeptide composition comprises nonapeptide-1, carnosine and tetrapeptide-30. According to the polypeptide composition disclosed by the invention, a novel formula is formed through adjusting ratios of differentpolypeptide components, so that polypeptide effects with different focuses and different applicable ranges; the monarch, minister, assistant and guide thought in traditional Chinese medical science isapplied to the polypeptide composition, so that the formula is diversified and the maximum applicability and applicable range are realized.

The invention mainly aims to provide a porcine circovirus type 2 (PCV2) subunit vaccine and a preparation method thereof. Particularly, a baculovirus vector expression system is utilized to express a large amount of recombinant open reading frame type 2 (ORF2) protein in insect cells, so that the subunit vaccine with good immunity effect is developed. A bac-to-bac baculovirus expression system is adopted to perform whole gene amplification on the porcine circovirus type 2 ORF2 gene, and a melittin signal peptide nucleotide sequence is introduced into a terminal 5', so that the recombinant baculovirus of an open reading frame containing the melittin signal peptide nucleotide sequence and a PVC2ORF2 gene sequence is established, wherein the infected insect cell expresses the recombinant ORF2 protein with efficient and high PCV2 antigenicity; and thus the subunit vaccine containing the PCV2 recombinant ORF2 protein is prepared. The inoculation experiments of piglets show that the subunit vaccine has a good immunity protection effect.

The invention relates to the field of medicine preparation, in particular to a melittin lipidosome preparation. The lipidosome preparation is composed of one part of melittin, 5-40 parts of phospholipid, 1.3-10 parts of cholesterin and 10-140 parts of poloxamer. The invention also discloses the preparing method of the melittin lipidosome preparation. The melittin lipidosome preparation not only can delay the medicine release in the lipidosome, prong the circulating time in the blood and improve the bioavailability of the medicine, but also can obviously reduce the side effect of the medicine and improve the adaptability of a patient.

The invention discloses a recombinant melittin and an application thereof, and belongs to the technical field of genetic engineering. An amino acid sequence of the recombinant melittin protein is shown in the formula SEQ ID NO.1. The recombinant melittin protein sequence can be utilized for a preparation of gene medicines utilized for preventing and treating fallopian tubal diseases of breeding hens, in other words, the recombinant melittin protein sequence is connected with a carrier pVAX1 to form a recombinant melittin expression carrier. In addition, a CMV promoter of the carrier pVAX1 is replaced by a chicken ovalbumin gene end 5' regulatory sequence which is an ov sequence, thus the recombinant melittin can be expressed specifically in chicken fallopian tubal tissue. The recombinant melittin and the gene medicines have the advantages of good effects of treating fallopian tubal diseases of breeding hens, safety, no toxicity and broad application prospect.

The invention provides a fusion protein formed by the combination of urokinase-type plasminogen activator a chain and melittin, the preparation method thereof, and the application of the fusion protein in tumor treatment.

The invention belongs to the technical field of medicines and discloses melittin complex nanoparticles for oral administration and a preparation method thereof. Water-soluble melittin and an amphiphilic substance are dissolved into a hydrosolvent A to form a hydrophobic protein ion pairing complex; the complex and a suitable polymer material are dissolved into a nonaqueous solvent B; an emulsion solvent diffusion method in a liquid phase is adopted to wrap the complex into the polymer material; and microparticles with the particle diameter between 10 and 1000nm are formed after the solvent is volatilized. The hydrosolvent A is distilled water, double distilled water, deionized water, physiological saline, or a phosphate buffer solution or an acetate buffer solution or a Tris buffer solution with the pH value of between 1 and 11; and the nonaqueous solvent B is a single or mixed solvent of alcohol, acetone, ethyl acetate, methylene dichloride, chloroform and dimethyl sulfoxide. The complex has high fat solubility, and the encapsulation rate of the nanoparticles is more than 90 percent. The preparation process is mild and can assure the biological activity of medicines. The preparation method is suitable to prepare oral administration preparations.

The invention discloses a fusion protein of melittin and human mutant interleukin-2, which is characterized in that, a linker peptide gene is used to link the encoding genes of human mutant IL-2 and melittin. The invention can be used to screen cloning capable of improving CD8+T cell mediated CTL reactivity of the body after the tumor formation, the fusion protein can be used fine genetic medicament for the treatment of tumors.

The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na⁺, K⁺, Cl⁻, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.

The invention discloses a polypeptide carrying melittin, nano-particles carrying melittin, and applications thereof. The invention belongs to the field of biological science and drug carriers. The peptide is composed of alpha helical polypeptide, connection sequence, and melittin which are connected in series with a covalent bond form. The nano-particles are formed through three effective bonding manners of polypeptides, phospholipids, and cholesterol esters carrying melittin. With the nano-particles prepared from the polypeptide carrying melittin, toxic and side effects of melittin against bodies can be effectively reduced, and effects can be performed specifically at diseased areas such as tumors. The nano-particles can be used in clinical treatments.

The invention belongs to the technical field of drugs produced by gene engineering in the biotechnological pharmacy industry and in particular relates to an antibacterial peptide. A primary structure of the antibacterial peptide is CLPLLISWIKRKRQQ-AGP-GSKKPVPIIYCNRRTGKCQRM. The newly designed antibacterial peptide simultaneously has the advantages of thanatin and melittin, not only has wide antibacterial spectrum but also can resist staphylococcus aureus and simultaneously avoids the disadvantage that melittin has strong hemolysis.

The invention discloses a non-viral gene vector constructed on the basis of N-terminal octadecane acylated antibacterial peptide. The vector utilizes the characteristics of melittin, i.e. endosome-destroying activity and membrane penetrating activity, moreover, a nuclear localization sequence and a polyarginine sequence are added into the melittin sequence, and then a series of forward and reverse analogues are synthesized, and octadecanoic acid is connected with the N terminals of the obtained analogues, so that the analogues and plasmid DNA (deoxyribonucleic acid) form stable nano-particles. An experiment result shows that because the non-viral gene vector constructed on the basis of N-terminal octadecane acylated antibacterial peptide has higher transfection efficiency and lower cytotoxicity and the polypeptide vector is easy to biodegrade, the non-viral gene vector is more suitable for application in vivo.

The invention provides an acid-activated CSP targeted antimicrobial peptide, which is prepared by reacting 2,3-dimethylmaleic anhydride (DMMAn), an antimicrobial peptide melittin and a CSP targeted molecule. Moreover, the invention provides a preparation method and two applications of the acid-activated CSP targeted antimicrobial peptide. According to the acid-activated CSP targeted antimicrobial peptide, the selectivity of the antibacterial peptide and the aggregation degree of the antibacterial peptide on the surface of SM (Streptococcus Mutans) bacteria can be improved through the CSP targeted molecule, and the toxicity of the antibacterial peptide melittin is lowered through the protection of the DMMAn on amidogens; and after entering a decayed tooth, the CSP targeted antibacterial peptide can be activated by the acid condition of a microenvironment, so that the CSP targeted antibacterial peptide has sterilizing activity.

The invention provides a plant expression vector for controlling an artificially synthesized antimicrobial peptide gene by using a specific vascular promoter and a method for culturing anti-greensickness cotton by using the same. In the method, an artificially synthesized antimicrobial peptide gene spCEMA (Signal Peptide modified Cecropin A-Melittin) under the control of a specific vascular promoter AAP2 is integrated to a cotton genome to realize the specific expression of the gene spCEMA in a cotton vascular tissue, so that the disease resistance of cotton to greensickness is obviously improved. By inoculating different pathogenic bacteria of different greensickness types to genetically modified cotton obtained with the method in a homozygosis plant infected field, a disease index is less than 15, which is reduced by more than 85% in contrast with that of non-genetically modified cotton.

The invention discloses a nano reagent based on melittin and a preparation method and an application thereof. The nano reagent is prepared by melittin, an organic dye, and hyaluronic acid. The preparation of the nano reagent is simple, hemolytic activity of melittin is greatly reduced, and the nano reagent can realize in-vivo tumour fluorescence imaging and obvious antineoplastic curative effect.The nano reagent can be effectively enriched at a tumour area after being subjected to intravenous injection in body, and can track the distribution condition of the nano reagent in the body through afluorescence signal, besides the chemotherapeutic anticancer activity of melittin, the organic dye components in the nano reagent can generate response on laser irradiation and increases the anticancer effect, so that the chemotherapy and phototherapy-combined for anticancer can be realized. In addition, the bio-security of the agent is good, the nano reagent can be widely used for preparing in-vivo cancer diagnosis and treatment-integrated medicines, and in-vivo treatment for melittin is realized.

The invention discloses a preparation method of hydrogel nanoparticles capable of adsorbing melittin and a detection method of affinity of the hydrogel nanoparticles. The preparation method comprises the steps that N,N-diethylacrylamide, acrylic monomers, a cross-linking agent and an emulsifier are dissolved in a container through deionized water according to proportions, nitrogen is blown into the container at room temperature to remove oxygen, magnetic stirring is conducted, the reaction temperature is raised to 60-80 DEG C, heat preservation is conducted for 20 min-60 min, the nitrogen atmosphere is maintained, an initiating agent is added to trigger a polymerization reaction, dialysis is conducted, and a hydrogel nanoparticle solution is obtained. The detection method comprises the step that hemdytic effect studies are adopted to detect the affinity between the hydrogel nanoparticles and the melittin. According to the preparation method of the hydrogel nanoparticles capable of adsorbing the melittin and the detection method of the affinity of the hydrogel nanoparticles, synthesis processes are all completed in an aqueous phase, the used monomers and auxiliaries are all common reagents, green and environmentally friendly, and the methods are easy to achieve, so that the hydrogel nanoparticles (NPs) have the advantages of being low in price and easy to obtain. The prepared hydrogel nanoparticles have an obvious adsorption effect on the melittin.

The invention discloses antibacterial peptide LL-37-Melittin, an application of the antibacterial peptide, and a preparation method of the antibacterial peptide in bacillus subtilis. The method comprises the steps of expressing the antibacterial peptide LL-37-Melittin in the bacillus subtilis by utilizing a fusion technology, manually designing a fusion gene SUMO-LL-37-Melittin, adding EcoRI and BamHI restriction enzyme cutting sites at the two ends, connecting the synthetized fusion gene and an expression vector pGJ148, successfully constructing a recombinant expression vector, transforming the recombinant expression vector into the bacillus subtilis WB800N to obtain an expression engineering bacterium, performing maltose induction expression in a culture medium, purifying an expression product by Ni-NAT affinity chromatography, detecting the correctness of the expression product by utilizing SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), and performing SUMO protease restriction enzyme cutting to obtain the antibacterial peptide LL-37-Melittin. The method expresses the antibacterial peptide in the bacillus subtilis; separation and purification are simple; and the method is easy to operate. The expression product has antibacterial activity for multiple bacteria, is good in thermal stability, is suitable for large-scale industrial production, and can be applied in the fields of medicines, food, animal husbandry and the like.

The invention relates to a novel melittin variant and an application thereof. At current, overuse of novel antibacterials and antibiotics is clinically to be developed, melittin as a good biology antibacterial agent is one of the best substitutes of the antibiotics. The inventor finds novel melittin based on the research. The melittin has good bacteriostasis and sterilizing capacity for Gram-negative bacteria, and through sequence improvement, the variant is obtained. Based on reservation of Gram-negative bacteria resistance of wild type melittin, good Gram-negative bacteria resistance is obtained. The novel melittin and the variant thereof provided by the invention have favorable clinical application prospects.

The invention provides a preparation method of a photosensitive nanocarrier for carrying melittin. The preparation method comprises the following steps that (1) nano-alumina particles are prepared; (2) biocompatible nano-metal particles are prepared; (3) the nanocarrier for carrying the melittin is prepared; and (4) a photosensitizer is added to obtain photosensitive nanoparticles, namely Al2O3@BSA@Melittin@Ce6, for carrying the melittin. The photosensitive nanocarrier, namely Al2O3@BSA@Melittin@Ce6, for carrying the melittin is applied to preparation of anticancer drugs. According to the preparation method of the photosensitive nanocarrier for carrying the melittin, the preparation problem of the photosensitive nanocarriers for carrying the melittin can be solved, and meanwhile, the technical problem that normal cells take in nanoparticles insufficiently is solved.

The invention relates to the technical field of medicine and discloses a melittin pamoate capable of being continuously released and a preparation agent and a preparation method of the same. The melittin pamoate is used for treatment of rheumatic arthritis and scapulohumeral periarthritis, is capable of enabling effective constituent to be continuously released for one to four weeks, overcomes the shortcoming that solution type free melittin is short in plasma half-life and poor in stability and requires frequency injection, and improves adaptability of patients for pharmacy.

The present invention relates to a bee venom essence, which comprises melittin, propolis, panax notoginseng, frankincense, safflower, processed radix aconiti, processed radix aconiti kusnezoffii, rhizoma homalomenae, zanthoxylum schinifolium sieb.et zucc, common clubmoss herb, phryma leptostachya, borneol, camphor, menthol, capsaicin, chlorhexidine acetate, cinnamon oil, turpentine oil, and winter green oil. The bee venom essence of the present invention has beneficial effects of no allergy, no irritating, no toxic side effect, and good physical therapy effect.

The invention discloses bee biological toilet soap which belongs to the soaps which are used in human daily life. The formula comprises that: the compound of melittin, propolis and polyethylene glycol 400 and the compound substances of neat soap materials, spices and pigments are evenly mixed and then inputted into a molding press to be molded into toilet soaps with block shapes. Since the invention utilizes a main active ingredient-the melittin in bee venom and utilizes natural bioactive substances with extremely strong biological activity and a plurality of pharmacological and physiological functions, the invention has the functions of hemolysis, expanding blood vessels, threshing mast cells, strong cell membrane adsorption effects and exciting a pituitary-an adrenal system, the invention enables corticotrophin to release more, thereby the invention can play desensitization roles of antiphlogistic, antibacteron and immune response inhibitation, and the invention not only can inhibit allergic reaction of the propolis but also exert the function of complementary synergy by combining with the propolis. The toilet soaps which are produced by the formulation and the technique of the invention can reach the proposed aims.

The invention relates to the field of detection, in particular to a method for quantitatively detecting melittin by liquid chromatography-tandem mass spectrometry, which comprises the step of detecting a characteristic peptide fragment of melittin in a sample to be detected by liquid chromatography-tandem mass spectrometry, wherein the sequence of the characteristic peptide fragment is VLTTGLPALISWIK. The method has high accuracy, precision and sensitivity, is high in stability, is suitable for accurate quantification of melittin in complex matrixes, and has important practical significance for protecting rights and interests of melittin consumers and maintaining healthy development of the bee product industry.