25 results about "Melittin" patented technology

The invention discloses a modified melittin peptide, a product containing the modified melittin peptide and application, and belongs to the technical field of biological medicine. The amino acid sequence of the modified melittin is shown as Seq ID No: 2, the molecular weight of the modified melittin is 3123.5 Da, and the isoelectric point of the modified melittin is 12.64. The invention further discloses application of the modified melittin peptide in preparation of drugs or cosmetics for inhibiting inflammation and relieving skin erythema and itching. The invention also discloses application of the modified melittin peptide in preparation of drugs or cosmetics for enhancing cell activity and promoting tissue repair. The melittin modified peptide can significantly reduce the cytotoxicity of natural melittin and significantly enhance the anti-inflammatory activity and tissue repair activity of the melittin, has no sensitization and phototoxicity, and can provide key technical support for research and development of new-generation efficient and safe anti-inflammatory repair drugs, cosmetic products and related biological materials.

The present invention discloses a multifunctional biomimetic ferritin nanomaterial responsive to matrix metalloproteinases and its preparation method and application. The present invention modifies melittin on the surface of ferritin nanoparticles by a genetic engineering method, connects with a linker containing a matrix metalloproteinase 9 (MMP‑2 / 9) cleavage site, and obtains recombinant ferritin (SEQ ID NO: 6) expressed in Escherichia coli and self-assembled to form enzyme-responsive functionalized biomimetic nanoparticles. The present invention utilizes this method to connect melittin in series on ferritin nanoparticles, significantly reducing the toxicity of melittin, and responding to matrix metalloproteinases in the tumor microenvironment to kill tumor cells; At the same time, intravenous administration of melittin can also be achieved, with good therapeutic effects on both in situ and metastatic tumors.

The application relates to a high-purity melittin purification method using pH zone refining countercurrent chromatography. In order to overcome the defects of the prior art, a solvent system composed of 4-12% methyl tert-butyl ether, 42-50% n-butanol, 4-12% acetonitrile and 42-50% deionized water is used, a high-purity melittin solution is purified by using the pH zone refining countercurrent chromatography, and the finished high-purity melittin is obtained through concentration and drying. The application does not use solid fillers, does not cause loss to the sample, can almost recover all the melittin in the crude melittin, the yield is more than 90%, and the application is suitable for batch purification of high-purity melittin.

The invention provides a tumor-specific melittin / antibody double-blocking prodrug molecule and application thereof, the prodrug molecule is in a peripheral tissue environment, the activity of an antibody and melittin in the prodrug molecule is masked, and a double-blocking state is formed; in a tumor microenvironment, specific enzyme in the tumor microenvironment can cut the cleavable peptide chain in the connecting fragment, so that the antibody and the melittin in the prodrug molecule are released and the activity of the antibody and the melittin is recovered, and the antibody and the melittin are in a double-activation state. The double-blocking prodrug molecule provided by the invention effectively solves the problem of non-targeting toxicity of the antibody, can exert different anti-tumor mechanisms of toxin and the antibody, achieves a synergistic treatment effect, and improves the curative effect and the safety at the same time.

The invention provides a melittin polymer, which is formed by at least connecting disulfide bonds between two melittin variant molecules after a melittin variant is formed by mutating alanine at the fourth site of an amino acid sequence of a melittin monomer into cysteine and mutating isoleucine at the twentieth site into cysteine in the amino acid sequence of the melittin monomer. The preparation method of the melittin polymer comprises the following steps: obtaining a melittin variant through escherichia coli recombinant expression, and purifying and oxidizing the obtained melittin variant through a nickel column to obtain the melittin polymer, and carrying out gel filtration chromatography on the obtained melittin polymer to obtain the purified melittin polymer. The invention also provides an application of the melittin polymer, and the melittin polymer is used for preparing antibacterial drugs or antibacterial feed additives. The prepared melittin polymer can effectively reduce the toxicity to red blood cells, has relatively strong environmental responsiveness and stability, and can effectively prolong the antibacterial aging.

The invention relates to chlorotoxin-melittin nanoparticles as well as a preparation method and application thereof. The method comprises the following steps: weighing chlorotoxin, dissolving the chlorotoxin in a PBS buffer solution, adding 2-imino sulfane hydrochloride, fully mixing, and incubating at room temperature to form a CTX-SH solution; the preparation method comprises the following steps: weighing dimyristoyl phosphatidylcholine, adding a trichloromethane solution, uniformly mixing, then adding a cholesterol oleate solution and a phosphatidyl ethanolamine-polyethylene glycol 2000-maleimide solution, and sufficiently and uniformly mixing; drying the solution, resuspending with a PBS (Phosphate Buffer Solution), and fully dissolving by ultrasonic; adding a thiolated CTX-SH solution into the solution, incubating for 1-3 days, and carrying out ultrafiltration purification to form a CTX-NP nanoparticle solution; preparing a melittin solution, dropwise adding the melittin solution into the CTX-NP nanoparticle solution, incubating for 10-24 hours, and performing ultrafiltration purification. The invention has the advantages of targeting brain glioma, inhibiting the growth of in-situ brain glioma and improving the immunosuppressive microenvironment of brain glioma.

The invention discloses a self-repairing temperature-sensitive hydrogel loaded with Cypate and melittin and application of the self-repairing temperature-sensitive hydrogel. Cypate is wrapped with apoferritin, and obtained Cypate recombinant apoferritin nanoparticles are uniformly dispersed in chitosan / beta-sodium glycerophosphate hydrogel, so that the self-repairing temperature-sensitive hydrogel is constructed. Oxidized chondroitin sulfate is introduced, and the oxidized chondroitin sulfate and amino in chitosan can form Schiff base, so that the hydrogel can be endowed with a self-repairing characteristic, the mechanical strength of the hydrogel is improved, and the sustained release of a therapeutic agent is better controlled. Experimental results show that when the self-repairing temperature-sensitive hydrogel is injected to the periphery of a tumor, the self-repairing temperature-sensitive hydrogel shows excellent retention capacity, deep and continuous release of FC nanoparticles in the tumor is promoted, the photo-thermal treatment effect is improved, meanwhile, melittin is slowly and continuously released, the expression level of M1 macrophages can be specifically reduced, the M2 / M1 proportion is promoted, and the self-repairing temperature-sensitive hydrogel can be used for treating tumor tumors. And the immunosuppressive microenvironment is regulated and controlled, and the synergistic effect of the two shows a remarkable breast tumor resisting effect.

The present disclosure relates to a recombinant expression vector for secretion of melittin and an attenuated Salmonella strain transformed therewith, and provides an attenuated Salmonella strain transformed with a recombinant expression vector including an flgM gene and a melittin gene, an anticancer pharmaceutical composition including the attenuated Salmonella strain as an active ingredient, and a recombinant expression vector capable of improving the secretion ability of melittin.

The invention discloses a spore surface display vibrio parahaemolyticus VPOmpK-MeL recombinant bacillus subtilis as well as a construction method and application thereof. According to the recombinant bacillus subtilis, on the basis of an integrated plasmid pDG364, a recombinant vector which takes spore capsid protein CotY as anchoring protein and displays a fusion fragment of a VPOmpK antigen and bee venom peptide Melittin on the surface is constructed, recombinant protein is converted into a wild type bacillus subtilis 168 gene, and the obtained recombinant spore expressing OmpK-MeL protein has genetic stability; the bacterium directly displays antigen protein on the surface of a spore, does not need to be broken, and can be directly mixed with materials or drink water to immunize animals, so that the problems of difficult protein purification, tedious injection immunization process and the like are avoided; the spores have good stress resistance, so that the spores are easy to store and transport, and the cost is remarkably reduced; the strain can induce specific immune response, and the fusion expressed MeL plays an excellent role in enhancing immunity, so that a new way is provided for prevention and treatment of VP, and the strain can be applied to development of commercial vaccines.

The invention relates to the technical field of cyclopeptide drug synthesis, in particular to a method for synthesizing mirror cyclopeptide by using OaAEP1 enzyme under the assistance of thioester, which comprises the following steps: firstly, synthesizing a linear mirror cyclopeptide thioester precursor by using an N-fluorenylmethoxycarbonyl (Fmoc) solid-phase polypeptide synthesis method, and then generating the mirror cyclopeptide from the thioester precursor under the mediation of the OaAEP1 enzyme. According to the invention, the mirror image ring melittin (D-cMelittin) is synthesized by using OaAEP1 enzyme under the assistance of thioester for the first time, and the mirror image ring melittin (D-cMelittin) has high protease stability and good antibacterial activity.

一种蜂毒肽改造肽及其应用，涉及生物医药技术领域，本发明的蜂毒肽改造肽Melittin‑m1‑5为基于天然蜂毒肽Melittin进行结构改造获得的，其氨基酸序列为如EQ ID NO:1所示。实验证明，Melittin‑m1‑5在微摩尔浓度范围内细胞毒性显著低于天然蜂毒肽，也能够显著抑制脂多糖诱导的炎症反应，降低炎症因子的表达，并表现出降低的溶血性。本发明提供的蜂毒肽改造肽在降低细胞毒性和溶血性的同时，保持并增强了抗炎活性，具有良好的应用安全性和开发前景，为抗炎相关药物及外用制剂的研发提供新的候选分子。

The present application relates to the technical field of cyclic peptide drug synthesis, and particularly relates to a method for synthesizing mirror image cyclic peptide by using OaAEP1 enzyme with the assistance of thioester, which comprises the following steps: firstly, a linear thioester precursor of mirror image cyclic peptide is synthesized by using an N-fluorenylmethyloxy carbonyl (Fmoc) solid-phase polypeptide synthesis method; and then the thioester precursor can generate the mirror image cyclic peptide under the mediation of OaAEP1 enzyme. The present application first synthesizes mirror image melittin (D-cMelittin) by using OaAEP1 enzyme with the assistance of thioester, and the mirror image melittin has high protease stability and good antibacterial activity.

The invention discloses a soluble microcrystal with tightening and anti-wrinkle effects as well as a preparation method and application thereof, and belongs to the technical field of beauty and skin care. The soluble microcrystal comprises the following components: collagen, hyaluronic acid, modified hyaluronic acid, melittin, nicotinamide, linoleic acid, phospholipid, cholesterol and the like. Hyaluronic acid is modified through EDTA and polyethylene glycol diamine, and modified hyaluronic acid is obtained. The modified hyaluronic acid is good in biocompatibility, has a multi-branch structure and can form a three-dimensional cross-linked network structure among molecules or with collagen, the mechanical strength of the microcrystal needle body is greatly improved, the microcrystal is prepared through the two steps of conventional drying and freeze-drying, and the rapid water solubility and drug release property of the microcrystal are guaranteed. Liposome formed by linoleic acid, phospholipid and cholesterol contains moisture and effective components, and transdermal absorption of the effective components can be greatly promoted.

The application provides a kind of base hornet MP-B peptide site-directed mutation derivative antibacterial peptide and its preparation method and application, belong to biotechnology field, the sequence of antibacterial peptide is as shown in SEQ ID No.1.The application uses base hornet MP-B peptide as template, respectively, the serine of 5, 8 is replaced by threonine, the amino acid sequence formed as shown in SEQ ID No.1, and the C-terminal is acylated with-NH2, is synthesized by solid phase chemistry, and after reversed-phase high performance liquid chromatography purification and mass spectrometry identification, the preparation of the antibacterial peptide is completed, the application of the antibacterial peptide in preparing antibacterial drug for treating gram-negative bacteria and / or gram-positive bacteria and / or fungal infectious diseases is disclosed.The antibacterial peptide of the application has the characteristics of high activity, low toxicity and high stability, and has the application potential of becoming a high-efficiency antibiotic substitute.

The invention belongs to the technical field of biological medicines, and provides a recombinant adeno-associated virus particle containing a targeted transcriptional melittin gene as well as a preparation method and application of the recombinant adeno-associated virus particle. Wherein the expression cassette containing the targeted transcriptional melittin gene contains a human telomerase reverse transcriptase core promoter, a tetracycline response element, an optimized melittin gene, a ribosome insertion sequence and a human enterokinase light chain-melittin fusion gene. The invention aims to solve the problem of how to realize precise targeted therapy of tumors, effectively kill tumor cells, reduce damage to normal cells to the greatest extent, overcome the limitation of a traditional treatment method and solve the problem of packaging cell death caused by advanced transcription of melittin genes in a recombinant AAV packaging process. And it is ensured that melittin expressed in tumor cells has a natural sequence and activity, and a safer and more effective treatment choice is provided for tumor patients.

The invention belongs to the field of biological medicine, and discloses melittin nano-composite temperature-sensitive hydrogel (NCP) and application thereof in prevention of postoperative recurrence of breast cancer, chitosan-based hybrid hydrogel is used as a carrier, and melittin and vitamin E succinic acid polyglutamic acid self-assembled nanoparticles (M / E15) are loaded. The hydrogel can be instantly and locally administered after operation and is used for preventing postoperative recurrence of breast cancer. The NCP has thermosensitivity, injectability and tissue adhesiveness, can fill up a treatment window between operation and chemotherapy, and realizes rapid coverage of postoperative wounds and drug sustained release. By directly killing residual tumor cells, inducing death of immunogenic cells, regulating Th1 / Th2 / Th17 / Treg cell differentiation and promoting M1 type polarization of macrophages, an immunosuppression microenvironment is reversed, so that tumor recurrence is inhibited. Meanwhile, NCP can inhibit release of proinflammatory factors, promote angiogenesis and skin remodeling and accelerate wound healing. In an advanced rupture model, the NCP and the PD-L1 antibody are combined for use, so that the anti-tumor effect can be synergistically enhanced, and wound repair can be promoted.

The invention relates to the technical field of bee breeding, in particular to application of a metabolite, a transcriptional gene or a microorganism as a marker for sophora flavescens alkalosis of bees. According to the invention, a plurality of markers related to the sophora flavescens alkalosis of bees are obtained through analysis of microorganisms, transcriptomes and metabolites, including a plurality of differentially expressed metabolites, transcriptional genes or microorganisms, and can be used for detecting the sophora flavescens alkalosis of bees, and the markers, especially metabolites of arginine succinic acid, spermidine or arachidonic acid, can be used for detecting the sophora flavescens alkalosis of bees. The transcription gene LOC107998471 and the microorganisms such as Gilliamella, Dorea and Lactobacillus plantarum are most remarkable, and the transcription gene LOC107998471 and the microorganisms such as Gilliamella, Dorea and Lactobacillus plantarum are most remarkable. In addition, the sophocarpidine poisoning of the bees can be relieved by feeding spermidine, and the method has important application value in the field of bee breeding.

The present invention relates to a choline-based melittin composite ionic liquid preparation and its preparation method and application. The choline-based melittin composite ionic liquid preparation comprises a composite ionic liquid and melittin formed by a choline 4-hydroxythiobenzamide ionic liquid and a choline lipoic acid ionic liquid in a certain mass ratio. The composite ionic liquid formed by the choline 4-hydroxythiobenzamide ionic liquid and the choline lipoic acid ionic liquid is selected as a carrier, and melittin with poor stability and transdermal absorbability is dissolved therein, which not only ensures the original efficacy of melittin, but also improves its skin permeability and stability. In addition, the choline-based melittin composite ionic liquid preparation has multifunctionality, exerts cascade antioxidant and anti-inflammatory effects with melittin, and can effectively regulate the body's immune response, and has broad application prospects in autoimmune diseases, inflammatory skin diseases and allergic diseases.

The present invention relates to a novel synthesis method for iodine-based nanoparticles and, more specifically, to iodine-based nanoparticles manufactured by electron beam irradiation, a method for manufacturing same, and a medical use thereof. Unlike conventional chemical synthesis methods, the method for manufacturing iodine-based nanoparticles, according to the present invention, can rapidly mass-produce small- and uniform-sized nanoparticles with a simple synthesis method using electron beam irradiation, and the iodine-based nanoparticles manufactured thereby are loaded with melittin and conjugated with transferrin, and thus can be used as a contrast agent for diagnostic imaging, as a drug delivery platform targeting a transferrin receptor, and as a therapeutic agent capable of treating vascular diseases by delivering drugs such as melittin.

The invention belongs to the technical field of biochemistry, and particularly relates to melittin-derived alicyclic peptide, which is prepared by replacing 2-site isoleucine and 26-site glutamine in a melittin peptide chain with 2, 4-diaminobutyric acid (Dab), coupling butyric acid, hexanoic acid, octanoic acid or decanoic acid on 1-site glycine (Gly) at a nitrogen terminal, and performing dehydration condensation and cyclization on the 2-site Dab and the 26-site Dab. Compared with a parent melittin, the melittin-derived alicyclic peptide disclosed by the invention has a better inhibition effect on pet-derived gram positive and negative germs, has lower hemolysis and cytotoxicity, shows better stability in in-vitro pepsin and trypsin solutions, and can be used for preparing the melittin-derived alicyclic peptide. The compound can be used for preparing novel anti-bacterial infection medicines, feed additives, in-vitro sprays and the like for pets, and has a very wide application prospect.

The invention provides a crissum nursing composition containing antibacterial peptide and a preparation method, and belongs to the technical field of composition medical nursing. The perianal care composition is prepared from the following raw materials: silk fibroin hydrolysate, carbomer, hyaluronic acid, glycerol, phenoxyethanol, cecropin, melittin, scorpion peptide ABP-W1 and deionized water. According to the crissum care composition, through the synergistic effect of the cecropin, the melittin, the silk fibroin, the carbomer and the hyaluronic acid, a protective film with antibacterial and bacteriostatic, physical barrier, moisturizing and repairing and anti-inflammatory functions is formed in the crissum, and crissum skin mucosa injury and infection caused by diarrhea, radiotherapy and chemotherapy, diaper stimulation and the like can be effectively prevented and treated; the traditional Chinese medicine composition has good curative effect and safety, and is suitable for infants, old people, radiotherapy and chemotherapy sensitive people and the like.

The invention belongs to the technical field of biochemistry, and particularly relates to a melittin analogue containing hydroxyl phosphorylated non-natural amino acid, and the melittin analogue is obtained by replacing pepsin enzyme cutting site amino acid and / or trypsin enzyme cutting site amino acid in a natural melittin peptide fragment with hydroxyl phosphorylated non-natural amino acid. Compared with melittin, the melittin analogue disclosed by the invention has a better inhibiting effect on pet-derived gram positive and negative germs, has lower hemolytic activity, shows better stability in in-vitro pepsin, trypsin and plasma, and can be used for preparing a pepsin analogue. The method has a very wide application prospect in the aspects of preparation of antibacterial infection drugs, feed additives, antibacterial in-vitro spray and the like for pets.

The application belongs to the technical field of biochemistry, and particularly relates to a melittin analogue containing a hydroxylated non-natural amino acid. The melittin analogue is obtained by replacing the amino acids at the pepsin cleavage site and / or the amino acids at the trypsin cleavage site in a natural melittin peptide segment with a hydroxylated non-natural amino acid. Compared with melittin, the melittin analogue of the application has better inhibitory effect on pet-derived gram-positive and gram-negative pathogenic bacteria, has lower hemolytic activity, and has better stability in pepsin, trypsin and plasma in vitro. The melittin analogue has very broad application prospects in the preparation of pet antibacterial infection drugs, feed additives and antibacterial in-vitro sprays.

The invention relates to the technical field of novel biomedical materials, in particular to lipidated melittin and application of the lipidated melittin as a vaccine vector, and the lipidated melittin is obtained by sequentially modifying the N end of melittin with lysine, phenylalanine and C8-C18 alkyl carboxylic acid. The lipidated melittin has good mRNA wrapping, delivery and immune response enhancing capabilities, is simple to prepare and can be used as a carrier of a nano vaccine for preparing the nano vaccine, the prepared nano vaccine has a good immune cell activation effect after being injected in vivo, and compared with a control group, the lipidated melittin can effectively prevent and inhibit tumor growth and has a good application prospect. And a valuable vector tool is provided for the development of mRNA tumor vaccines.

The present invention discloses a production process of melittin, belonging to the technical field of polypeptide synthesis, and specifically relates to a method for preparing melittin. The method comprises synthesizing a melittin-peptide resin by solid-phase synthesis in the order of the melittin sequence, and cutting and purifying the melittin. The solid-phase synthesis method adopts coupling amino acid reagents one by one or coupling using at least one melittin peptide segment and a single amino acid reagent, and the number of amino acids in the melittin peptide segment is 4-8. In the present invention, the synthesis of the melittin peptide segment adopts a liquid-phase synthesis carrier, which is synthesized from 2,4-dihydroxybenzaldehyde, 1,4-dibromocyclohexane and diethyl bromomalonate and obtained by reduction.