108 results about "Melittin" patented technology

The invention discloses a preparation method of hydrogel nanoparticles capable of adsorbing melittin and a detection method of affinity of the hydrogel nanoparticles. The preparation method comprises the steps that N,N-diethylacrylamide, acrylic monomers, a cross-linking agent and an emulsifier are dissolved in a container through deionized water according to proportions, nitrogen is blown into the container at room temperature to remove oxygen, magnetic stirring is conducted, the reaction temperature is raised to 60-80 DEG C, heat preservation is conducted for 20 min-60 min, the nitrogen atmosphere is maintained, an initiating agent is added to trigger a polymerization reaction, dialysis is conducted, and a hydrogel nanoparticle solution is obtained. The detection method comprises the step that hemdytic effect studies are adopted to detect the affinity between the hydrogel nanoparticles and the melittin. According to the preparation method of the hydrogel nanoparticles capable of adsorbing the melittin and the detection method of the affinity of the hydrogel nanoparticles, synthesis processes are all completed in an aqueous phase, the used monomers and auxiliaries are all common reagents, green and environmentally friendly, and the methods are easy to achieve, so that the hydrogel nanoparticles (NPs) have the advantages of being low in price and easy to obtain. The prepared hydrogel nanoparticles have an obvious adsorption effect on the melittin.