3 results about "Melittin" patented technology

The application relates to a high-purity melittin purification method using pH zone refining countercurrent chromatography. In order to overcome the defects of the prior art, a solvent system composed of 4-12% methyl tert-butyl ether, 42-50% n-butanol, 4-12% acetonitrile and 42-50% deionized water is used, a high-purity melittin solution is purified by using the pH zone refining countercurrent chromatography, and the finished high-purity melittin is obtained through concentration and drying. The application does not use solid fillers, does not cause loss to the sample, can almost recover all the melittin in the crude melittin, the yield is more than 90%, and the application is suitable for batch purification of high-purity melittin.

一种蜂毒肽改造肽及其应用，涉及生物医药技术领域，本发明的蜂毒肽改造肽Melittin‑m1‑5为基于天然蜂毒肽Melittin进行结构改造获得的，其氨基酸序列为如EQ ID NO:1所示。实验证明，Melittin‑m1‑5在微摩尔浓度范围内细胞毒性显著低于天然蜂毒肽，也能够显著抑制脂多糖诱导的炎症反应，降低炎症因子的表达，并表现出降低的溶血性。本发明提供的蜂毒肽改造肽在降低细胞毒性和溶血性的同时，保持并增强了抗炎活性，具有良好的应用安全性和开发前景，为抗炎相关药物及外用制剂的研发提供新的候选分子。

The application provides a kind of base hornet MP-B peptide site-directed mutation derivative antibacterial peptide and its preparation method and application, belong to biotechnology field, the sequence of antibacterial peptide is as shown in SEQ ID No.1.The application uses base hornet MP-B peptide as template, respectively, the serine of 5, 8 is replaced by threonine, the amino acid sequence formed as shown in SEQ ID No.1, and the C-terminal is acylated with-NH2, is synthesized by solid phase chemistry, and after reversed-phase high performance liquid chromatography purification and mass spectrometry identification, the preparation of the antibacterial peptide is completed, the application of the antibacterial peptide in preparing antibacterial drug for treating gram-negative bacteria and / or gram-positive bacteria and / or fungal infectious diseases is disclosed.The antibacterial peptide of the application has the characteristics of high activity, low toxicity and high stability, and has the application potential of becoming a high-efficiency antibiotic substitute.