180 results about "Bee venom" patented technology

The invention discloses Melittin, a micro-molecular polypeptide of amino acid type antibacterial / mAb with the formula of INLKAIAALAKKLL-NH2. The invention can be applied for the preparation of medicaments for eradicating bacteria and treating pyaemia.

The present invention belongs to the field of biomedicine technology. Wasp antibiotic peptide is a kind of single-stranded polypeptide separated from wasp venom and with molecular weight of 1316.6 dalton, isoelectric point of 8.59, and polypeptide whole sequence of NH2-IDWKGIAAMAKI-COOH. It is prepared through electrically stimulating wasp to collecting wasp venom, centrifuging to eliminate precipitate, freeze drying, chromatographic separation and purification in gel column, ion exchange column and reverse high pressure liquid phase chromatography. The wasp antibiotic peptide has powerful activity of inhibiting bacteria, fungi, viruses and tumor cell growth and the advantages of no hemolysis activity, etc. and may be used in preparing medicine for treating pathogeneic microbial infection disease and tumor.

The present invention relates to methods of prophylactically treating, reducing, delaying or controlling severe allergic reaction, e.g., to food and / or hymenoptera allergen, e.g. peanut allergen or bee venom allergen, in patients at risk of systemic anaphylaxis, e.g., patients receiving allergen desensitization therapy, through the use of a mast cell stabilizer, e.g., ketotifen and / or its metabolites or derivatives in free or pharmaceutically acceptable acid addition salts thereof. The invention further provides methods to desensitize a patient to one or more allergens comprising administering (i) one or more allergen to induce tolerance and (ii) one or more mast cell stabilizers in free or pharmaceutically acceptable salt thereof.

The invention belongs to the technical field of medicines and discloses melittin complex nanoparticles for oral administration and a preparation method thereof. Water-soluble melittin and an amphiphilic substance are dissolved into a hydrosolvent A to form a hydrophobic protein ion pairing complex; the complex and a suitable polymer material are dissolved into a nonaqueous solvent B; an emulsion solvent diffusion method in a liquid phase is adopted to wrap the complex into the polymer material; and microparticles with the particle diameter between 10 and 1000nm are formed after the solvent is volatilized. The hydrosolvent A is distilled water, double distilled water, deionized water, physiological saline, or a phosphate buffer solution or an acetate buffer solution or a Tris buffer solution with the pH value of between 1 and 11; and the nonaqueous solvent B is a single or mixed solvent of alcohol, acetone, ethyl acetate, methylene dichloride, chloroform and dimethyl sulfoxide. The complex has high fat solubility, and the encapsulation rate of the nanoparticles is more than 90 percent. The preparation process is mild and can assure the biological activity of medicines. The preparation method is suitable to prepare oral administration preparations.

The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl−, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot or dye by directly absorbing its fluorescence emission.

The invention discloses a bee venom composition with a wrinkle removing effect. The composition comprises wrinkle removing cosmetic matrix components and bee venom. The bee venom is applied to wrinkle removing cosmetics for the first time, and can act on the epidermis, the dermis and the muscle tissues at the same time to realize deep wrinkle removal. By using good synergistic effect of the bee venom, hyaluronic acid and marine animal collagen peptide, the effects of moisturizing, improving dryness and laxity of skin, improving the elasticity of the skin, deeply removing wrinkles and the like are remarkably enhanced. The bee venom composition with the wrinkle removing effect is definite in effect component, stable in quality and diverse in variety, and can meet the requirements of different genders, ages and skin types.

A method for refining bee venom comprises the steps: preparing the bee venom, adding water of 5-10 times of the amount of the bee venom into the bee venom to perform dissolution and then perform high speed centrifugation, and water phase is obtained after the centrifugation; adding acetone with the 1-3 times of the volume of the water phase into the water phase to perform standing at the temperature of less than or equal to -8 DEG C until the precipitation finishes completely, adding the acetone in precipitate and smashing the mixture after separation, performing filtering separation and volatilizing the acetone in the precipitate to obtain a rough bee venom product; using 0.08-0.12mol / L of acetum to dissolve the rough product, then using a sephadex G50 chromatographic column to perform the separation and purification, using acetum with the same concentration to perform elution and using an ultraviolet detector to perform on-line detection at 280nm of wavelength, collecting eluent of a third peak, adding activated carbon to perform slow stirring, and freezing and drying filtrate after the separation. In the refined bee venom, melittin content is greater than or equal to 76%, macromolecule substance content is less than 1.5%, and a lyophilized agent for clinical injection can be directly added.

The present invention belongs to the field of biomedicine technology. Wasp antibiotic peptide is a kind of single-stranded polypeptide separated from wasp venom and with molecular weight of 1316.6 dalton, isoelectric point of 8.59, and polypeptide whole sequence of NH2-IDWKGIAAMAKI-COOH. It is prepared through electrically stimulating wasp to collecting wasp venom, centrifuging to eliminate precipitate, freeze drying, chromatographic separation and purification in gel column, ion exchange column and reverse high pressure liquid phase chromatography. The wasp antibiotic peptide has powerful activity of inhibiting bacteria, fungi, viruses and tumor cell growth and the advantages of no hemolysis activity, etc. and may be used in preparing medicine for treating pathogeneic microbial infection disease and tumor.

The invention discloses a polypeptide carrying melittin, nano-particles carrying melittin, and applications thereof. The invention belongs to the field of biological science and drug carriers. The peptide is composed of alpha helical polypeptide, connection sequence, and melittin which are connected in series with a covalent bond form. The nano-particles are formed through three effective bonding manners of polypeptides, phospholipids, and cholesterol esters carrying melittin. With the nano-particles prepared from the polypeptide carrying melittin, toxic and side effects of melittin against bodies can be effectively reduced, and effects can be performed specifically at diseased areas such as tumors. The nano-particles can be used in clinical treatments.

The invention relates to a traditional Chinese medicine disinfectant and a preparation method thereof. The raw materials for preparing effective components of the disinfectant comprise: fructus piperis longi, lithargite, dendrobium, bee venom, emblic leafflower leaf, xanthorrhiza, saururus chinensis, the bark of ash, camphortree leaf, swallow tail, nephelium lappaceum, mile swertia, fungus-infected rice spike, chicory, folium sennae and greater celandine. The traditional Chinese medicine disinfectant disclosed by the invention is strong in bactericidal ability, high in acting speed, strong in stability, free of stimulation on skin of a human body, easy to dissolve into water, free of residue after use, safe and free of toxic and side effects, can be widely applied to sterilization of skin of clinical laboratory personnel, medical apparatus and instruments, medical places and the like, and is an ideal disinfectant worth spreading.

The present invention relates to a composition for preventing and treating acne, comprising a material which has excellent antibacterial activity against Propionibacterium acnes known as acne-causing bacteria and which shows strong antibacterial activity against skin floras that enlarge the site of inflammation to worsen acne, in which the material also helps to regenerate skin cells damaged by acne. The composition comprises bee venom (collected from Apis meliffera) and is used the form of soap, cosmetic products, and medical products for skin application such as ointments, bands and dressings, which are used to fundamentally prevent and treat acne.

The invention discloses infantile convulsions tincture for dredging channels and expelling wind-damp. The specific formula consists of 0.05 g of dried bee venom, 5 g of propolis, 15 g of natural Chinese herbal medicine-radix clematidis, 5 g of flowers carthami, 5 g of pseudo-ginseng, 15 g of lycopodium clavatum, 10 g of sappanwood, 8 g of radix curcumae longae, 15 g of angelica sinensis, 5 g of sangusis draconis and 2 g of borneol; coarse powder is prepared, is dipped in 60 degrees of white spirit for termly stirring, and is then stood; supernate is poured out for future use; medical alcohol is added in remainder precipitates for stirring and standing; the supernate is poured out; the supernate collected by the two times is mixed, and is filtered by a nylon yarn for preparing the tincture; the tincture is filled in a watering can for future use. The infantile convulsions tincture for dredging channels and expelling wind-damp has no toxic and side effects on human bodies, makes up one blank of external tincture, has the functions of relaxing muscles, stimulating blood circulation, dredging channels and dispersing blood stasis, has excellent effect on the external use of itching kind skin diseases, has simple preparation process, and has excellent curative effect on joint injury, women dysmenorrheal, nerve headache, limbs chills, cat fever, abnormal skin color, waist pain and children fever.

The invention discloses a method for preparing a bee venom injection, which comprises the following steps of: (a) weighing bee venom, water for injection and octanol in a mass-to-volume ratio of 1:(20-30):(5-7); (b) dissolving the bee venom in the water for injection and then adding the octanol for extraction; separating extract liquor by using a sephadex column; and (c) freezing the bee venom for 1-2h at the temperature ranging from -10 DEG C to -15 DEG C; diluting the frozen bee venom solution to an specific amount with a prepared buffer solution; carrying out ultrafiltration by using ultrafiltration equipment, adding 0.05-0.1% of glycine, stirring, carrying out rough filtration and fine filtration and finally packaging. The bee venom injection prepared by the method has the advantage of stable quantity and high percent of pass.

The invention relates to a bee-venom-peptide skin-tone perfecting firming activating eye cream and a manufacturing method thereof, and relates to a manufacturing technique of a beauty article, i.e. an eye cream. Monoglyceride, silicon oil, jojoba oil, stearic acid and cocoon fiber oil are mixed and heated to the temperature of 70-75 DEG C, and are stirred and processed through heat preservation; vitamin E, bisaboll and polylol are heated to the temperature of 70-75 DEG C; the two groups of components are mixed and stirred; when the temperature is reduced to 40-50 DEG C, bee-brood freeze-dried powders, antioxidant, preservative, deionized water and essence are added in, and the mixture is uniformly stirred, so as to obtain the bee-venom-peptide skin-tone perfecting firming activating eye cream. The method has simple process, and the practical productive operation is convenient; by adopting the special technology, the bee-venom-peptide skin-tone perfecting firming activating eye cream can keep the self characteristic of the bee-brood freeze-dried powders effectively; under the combined action of the bee-brood freeze-dried powders and other components, the wrinkles of canthus and wrinkles around the eyes can be smoothed out obviously.

The invention relates to a special emplastrum prepared by mixing far-infrared ceramic powder with traditional Chinese medicinal micro-powder, active carbon micro-powder and a medical pressure-sensitive adhesive, and concretely relates to a bee venom emplastrum for blood circulation invigorating, vein relaxing and pain relieving. The bee venom emplastrum has an obvious local microcirculation improvement function, and is suitable for pains in neck, shoulder, waist and legs, hyperosteogeny, intervertebral disc protrusion and other symptoms. The bee venom emplastrum comprises a lining material, an emplastrum medicine layer and an emplastrum surface covering. The lining material is a nonwoven fabric; the emplastrum medicine layer is formed by the far-infrared ceramic powder, the active carbon micro-powder, the traditional Chinese medicinal micro-powder and the medical pressure-sensitive adhesive; and the emplastrum surface covering is a release paper or a release film. The bee venom emplastrum is prepared through the steps of stirring and mixing the above components, coating, cutting and packaging. The bee venom emplastrum has the characteristics of fast effectiveness, low allergy rate, use convenience and the like.

The present invention relates to a composition comprising the purified extract of bee venom for preventing and treating degenerative brain diseases and the use thereby. The purified extract of bee venom shows potent inhibitory effect on microglial cell activation and abnormal circling behavior using by animal model of degenerative brain disease as well as potent cell-protective activity therefore, it can be useful in treating and preventing the degenerative brain disease as a medicament.

The invention discloses a bee venom compound with a sun-proof effect. The bee venom compound comprises a sun-proof cosmetic substrate component and bee venom. According to the invention, the bee venom is applied to sun-proof cosmetics for the first time, can effectively prevent ultraviolet ray from hurting skin, relieve scytitis caused by sunburn, and meanwhile enable the skin to keep humidification, provide nutrient for the skin, prompt the rehabilitation of hurt skin, and improve the self-protecting ability of the skin. And in addition, according to the invention, the bee venom, algal polysaccharides and Vc phosphate magnesium are compounded, thus the effects (including sun resistance, diminish inflammation, repair after being exposed to the sun and the like) of the bee venom compound are enhanced. The bee venom compound with the sun-proof effect comprises cream, emulsion, water aqua, gelatum and jelly, and is suitable for crowd with various skin types.

The invention relates to application of biogenic amine serving as a feature mark in bee venom type identification and further relates to a method for identifying types of bee venoms through the biogenic amine as the feature mark by means of HPLC / MS / MS methods. According to the method, one or more of histamine, dopamine, serotonin, epinephrine and noradrenaline serves as the feature mark, one or more of Italian bee venom, royal jelly high-yielding bee venom, Anatolia bee venom, Caucasia bee venom, Carniolan bee venom and Carpathian bee venom can be precisely identified. The method can efficiently, stably and accurately identify six types of bee venoms, and has significance on reinforcing study on bee venom type identification, exploring bee venom traceability information, developing traditional Chinese medicine, improving bee venom quality in China and guaranteeing health of consumers.

The invention discloses a nano reagent based on melittin and a preparation method and an application thereof. The nano reagent is prepared by melittin, an organic dye, and hyaluronic acid. The preparation of the nano reagent is simple, hemolytic activity of melittin is greatly reduced, and the nano reagent can realize in-vivo tumour fluorescence imaging and obvious antineoplastic curative effect.The nano reagent can be effectively enriched at a tumour area after being subjected to intravenous injection in body, and can track the distribution condition of the nano reagent in the body through afluorescence signal, besides the chemotherapeutic anticancer activity of melittin, the organic dye components in the nano reagent can generate response on laser irradiation and increases the anticancer effect, so that the chemotherapy and phototherapy-combined for anticancer can be realized. In addition, the bio-security of the agent is good, the nano reagent can be widely used for preparing in-vivo cancer diagnosis and treatment-integrated medicines, and in-vivo treatment for melittin is realized.

Bee venom may be administered in a standardized formulation with or without relatively small amounts of anesthetic. In particular, the results of the combination of venom and anesthetic dramatically decreased pain and discomfort for patients undergoing apitherapy.

The invention relates to a preparation method of a bee venom essence. The preparation method comprises the following steps: S1: a bee venom taking device is prepared; S2: bee venom is collected; S3: bee venom is purified by separation of impurities and separation of allergens; S4: a propolis solution is prepared for standby by completely dissolving propolis with polyethylene glycol; S5: Chinese herbal medicines are uniformly mixed according to a formula proportion, the Chinese herbal medicines are decocted with water for twice, and water decoctions are merged; S6: bee venom is added into a proper amount of Tween 80 and is mixed with the propolis solution, and the propolis mixed liquor and the traditional Chinese medicine concentrate are mixed for standby; S7: wintergreen oil and other oil substances are added into the mixed liquor in order to obtain mixed liquor which is the bee venom essence finished product. The method has the beneficial effects that the preparation method of the bee venom essence has a simple process and a high efficiency.

The invention discloses an application of bee venom lipid nanoparticles, which relates to the technical field of biological science and a pharmaceutical carrier. The bee venom lipid nanoparticles of the invention are composed of bee venom hybrid polypeptide and phospholipid and cholersteryl ester. The immune activation and anticancer agent prepared by the bee venom lipid nanoparticles is used fortargeting antigen presenting cells, and activates antigen presenting cells to improve the immune microenvironment, so as to generate a systemic immune response and inhibit tumor growth and metastasiseffect; the agent is also used to target and activate dendritic cells, macrophages, and B cells to inhibit the production of distant tumors; and the agent is also used to target hepatic sinusoidal endothelial cells and induce them to release a variety of immune factors to inhibit tumor liver metastasis. The immune activation and anticancer agent prepared by the bee venom lipid nanoparticle has notoxic and side effects, does not depend on the tumor type, and has good anti-tumor effect.

A biodegradable aptoxin microsphere as injection is prepared through dissolving the biodegradable high-molecular material in dichloromethane or ethyl acetate, ultrasonic dispersion of the aqueous solution of aptoxin in it to obtain water-in-oil emulsion, cooling to 5 deg.C, dropping it to the aqueous solution of polyvinyl alcohol, stirring to obtain water-in-oil-in-water emulsion, continuous stirring to volatilize the dichloromethane or ethyl acetate and solidify the microspheres, filtering, washing and vacuum drying.

Bee venom injection liquid is the sterilization aqueous solution of bee venom and is used for remedying the rheumatoid or rheumatoid arthritis, the peripheral neuritis and the neuralgia. The present invention introduces a preparation method of the sterilization aqueous solution. The process of the preparation method is that aether is used for extraction after water is dissolved; the aether is volatilized out; sephadex gel G-50 is used for separating; a 0.1 million molecular weight hyperfiltration pole is used for hyperfiltration; the bee venom injection liquid can be obtained after aseptic filtration, encapsulation and sterilization. The bee venom injection liquid has the advantage of removing the inflammatory substance such as 5-hydroxytryptamine etc. and macromolecular protein in the bee venom, so as to weaken the excitability and the irritability of the bee venom injection liquid and improve the curative effect and stability of the bee venom injection liquid.

The invention discloses a recombinant virus secreting a multi-epitope gene expressing a porcine O-type foot-and-mouth disease virus, a preparation method thereof and application thereof. The gene sequence of a multi-epitope recombinant antigen of the porcine O-type foot-and-mouth disease fused with bee venom signal peptide is artificially designed according to the preference of a codon by connecting T and B cell epitopes of the porcine O-type foot-and-mouth disease virus, and a recombinant baculovirus containing the above recombinant gene is constructed to increase the secreting expression level and the immunogenicity of the gene in the baculovirus. A subunit vaccine is prepared by using an RBT protein expressed by the recombinant baculovirus Ac-RBT. After a pig is immunized, the body canbe induced to have specific immune reaction, and the pig body is induced to produce a higher level specific antibody which can effectively prevent the foot-and-mouth disease virus, and is of great significance for control and purification of the porcine O-type foot-and-mouth disease in China.

The invention relates to a novel melittin variant and an application thereof. At current, overuse of novel antibacterials and antibiotics is clinically to be developed, melittin as a good biology antibacterial agent is one of the best substitutes of the antibiotics. The inventor finds novel melittin based on the research. The melittin has good bacteriostasis and sterilizing capacity for Gram-negative bacteria, and through sequence improvement, the variant is obtained. Based on reservation of Gram-negative bacteria resistance of wild type melittin, good Gram-negative bacteria resistance is obtained. The novel melittin and the variant thereof provided by the invention have favorable clinical application prospects.

The invention discloses an external bee venom liniment, and belongs to the preparation and a method for an external Chinese medicinal herb preparation. The method comprises the following steps: smashing clove, safflower, frankincense, ningpo yam rhizome, common jasminorange leaf and twig, szechuan lovage rhizome, cinnamon and shichimi into fine powder; putting the fine powder and bee glue into a multi-functional automatic extractor; soaking the mixture by using 95 percent ethanol for 5 hours; performing automatic circulation and reflux extraction on the soaked mixture to obtain extract; vacuumizing and drying the extract and smashing the vacuumized and dried extract into fine powder; grinding borneol into fine powder; uniformly mixing the two fine powder and the bee venom; adding a solvent into the mixture, uniformly stirring and standing the mixture; and filtering the mixture to obtain a product. The liniment product prepared by scientific mixing has the effects of promoting blood circulation and removing blood stasis, expelling wind, removing dampness and dispersing cold, and reducing swelling and easing pain. The external bee venom liniment can be used for treating diseases, such as cervical and lumbar intervertebral disc protrusions caused by cold and damp stagnation and qi stagnation and blood stasis, herpes, partial edema pain and functional limitations and the like.