199 results about "Phospholipase" patented technology

The invention belongs to the field of enzymatic preparation of structural phospholipids, and particularly relates to a method for preparing phosphatidylserine through interfacial enzyme catalysis. A series of high-activity immobilized phospholipase are prepared by innovatively adopting bifunctional group modified hollow mesoporous silica microspheres as a carrier and adsorbing immobilized phospholipase D. The catalyst has the functions of a catalyst and an emulsifier, and a Pickering emulsion interfacial enzyme catalysis system can be efficiently constructed. In the system, the phosphatidylcholine and the serine are efficiently converted into the phosphatidylserine through a phosphatidyl transfer reaction, the conversion rate of the phosphatidylserine within 20 minutes can reach 85%-95%, and the catalytic efficiency reaches up to 372 mmol / (g.h). In addition, after the catalyst is recycled for 10 times, the conversion rate is still kept at 70.9%, and the catalyst shows excellent stability and reusability. The method has the remarkable advantages of being mild in reaction condition, high in catalytic efficiency, capable of being repeatedly used and the like, and a new technical approach is provided for industrial production of phosphatidylserine.

The disclosure encompasses genetically modified plants and methods for improving phosphorus redistribution and use efficiency. The genetically modified plants increase the expression of a nonspecific phospholipase C4 (NPC4) protein, enhancing phosphorus remobilization from old, senescing tissues to young, growing tissues and seeds. The genetic modification improves plant growth, increases seed and oil yield, and alters fatty acid composition. The genetically modified plants demonstrate enhanced phosphorus use efficiency under phosphorus-limited conditions, showing greater growth and yield improvements compared to wild-type plants. Specifically in camelina plants, the genetic modification increases seed yield, elevates oil content, and alters fatty acid composition of the oil under P-limited conditions.

The invention discloses a preparation method of a sandwich structure immunosensor based on surface enhanced Raman scattering and application of the sandwich structure immunosensor to detection of an anti-phospholipase A2 receptor antibody, and is characterized by comprising the following steps: sputtering silver ions on a cotton swab / ZIF-67 prepared by an in-situ growth method by adopting an ion sputtering method to obtain a cotton swab / ZIF-67 / Ag NPs composite material; the preparation method comprises the following steps: preparing an antigen solution, then adding the antigen solution into the antigen solution to obtain an immune substrate, adding a methylene blue aqueous solution into a silver nanoparticle solution for reaction, then connecting an anti-human PLA2R IgG antibody to obtain an immune probe, and finally dropwise adding a phosphate buffer solution containing an antibody to be detected onto the immune substrate, and dropwise adding the immune probe solution to the immune substrate adsorbed with the antibody to be detected to obtain the sandwich structure immunosensor based on surface enhanced Raman scattering. The method has the advantages of high sensitivity, good selectivity and strong stability.

The invention belongs to the technical field of food lipid and processing, particularly discloses a one-pot enzymatic cascade degumming and deacidification method for grease and application, and particularly relates to a method for performing cascade degumming and deacidification on multiple kinds of grease, generating functional lipid, improving the quality of the grease and maintaining the flavor of the grease. According to the method, phospholipase is immobilized through the porous carrier and is successfully applied to enzymatic cascade degumming and deacidification, and degumming, deacidification or cascade degumming and deacidification can be carried out on different raw materials according to requirements. Moreover, the prepared grease is rich in functional lipid components, the flavor is efficiently reserved, the oxidation stability is improved, the nutritional quality is improved, the immobilized carrier is cheap and easy to obtain, the immobilized enzyme can be repeatedly used for multiple times and is remarkably superior to free enzyme, and the industrial application potential is huge.

The invention discloses antioxidant salmon collagen peptide and a preparation method thereof, and belongs to the technical field of processing of salmon collagen peptide. The preparation method comprises the following steps: firstly, carrying out enzymolysis on the salmon material by using phospholipase A2, hydrolyzing abundant membrane phospholipids in the salmon material, destroying fat cell structures and releasing wrapped triglyceride, then carrying out secondary enzymolysis by using aspergillus oryzae lipase, hydrolyzing macromolecular lipids into free fatty acids which are easily dissolved in supercritical carbon dioxide, and carrying out secondary enzymolysis by using aspergillus oryzae lipase. The degreased salmon material is obtained by means of supercritical carbon dioxide extraction, the lipid removal rate is greatly increased, and the antioxidant salmon collagen peptide which is small in molecular weight and high in oxidation resistance is obtained through subsequent collagen extraction and enzymolysis.

Provided are a marine phospholipase C mutant and a use thereof. Compared with wild-type (WT) enzyme protein, at 40°C and under conditions where enzyme protein activity remains essentially unchanged, the enzyme protein half-life of the constructed mutant can be increased to 3.46 and 9.25 times that of the original enzyme protein, and the residual phosphorus content in degummed oil can be reduced from 551 ppm to 72 ppm, which shows a significant improvement over the final residual phosphorus content of 175 ppm for the blank control and the final residual phosphorus content of 131 ppm for the WT. These results demonstrate that the enzyme has improved thermal stability, enables oil degumming under various conditions, and is suitable for a broader range of industrial applications.

The embodiment of the specification provides an astaxanthin extraction device, the device comprises a mixing tank, a cavitator, a static mixer, a three-phase centrifuge and a precision filter, the mixing tank is configured to mix haematococcus pluvialis, water and phospholipase to form uniform algae slurry; the cavitator is configured to receive the algae slurry and perform wall breaking on the algae slurry by utilizing hydrodynamic cavitation to obtain wall-broken algae slurry; the static mixer is configured to mix the wall-broken algae slurry and an extraction agent to form mixed algae slurry; the three-phase centrifugal machine is configured to receive the mixed algae slurry and perform three-phase separation on the mixed algae slurry to obtain an astaxanthin oil phase, a water phase and algae residues; the precision filter is configured to filter the astaxanthin oil phase so as to remove residual algae residues, and the astaxanthin oleoresin is obtained.

The invention provides a targeted egg yolk cholesterol removal device and method, the device comprises a heating device, an infrared sensor, a liquid taking rod and other key components, and the temperature, layering position detection and supernate collection in the liquid treatment process can be accurately controlled. The whole process flow comprises the following steps: firstly, diluting the egg yolk with a NaCl solution, treating with phospholipase and performing enzyme deactivation treatment; then, determining a layering position by using an infrared sensor, and collecting supernate to a lower stirring tank. And then adjusting the pH value, and adding beta-cyclodextrin for further reaction. And finally, transferring the precipitate to a lower stirring tank through a high-pressure air pump, mixing the precipitate with the supernate, and performing centrifugal treatment to obtain the yolk with the cholesterol content greatly reduced. The phospholipase and cyclodextrin adopted in the invention are natural substances, have no toxic or side effect, and are safe and reliable. In the process of removing cholesterol, harmful substances are not generated, and the method is environment-friendly; the provided equipment is high in function integration degree, the number, cost and energy consumption of the equipment are reduced, and material loss is reduced.

Disclosed is a method for reducing the usage amount of edible fat and oil in a bakery product, the method comprising mixing at least one maltose alpha-amylase and edible fat and oil into dough, and baking same to prepare a bakery product. The usage amount of the edible fat and oil in the dough can be reduced by at least 10 wt % compared with not using the enzyme treatment; and the enzyme may also include cellulase and / or phospholipase. The method does not reduce or substantially reduce the quality of the bakery product, and allows same to have a shelf life of at least 4 days. Also involved is baked fat and oil prepared from the above-mentioned enzyme and edible fat and oil.

The present invention relates to compounds of Formula A and pharmaceutically acceptable salts thereof, their use in medicine; compositions containing them; processes for their preparation; and intermediates used in such processes. The compounds of the invention are useful for treating, preventing, inhibiting, and ameliorating diseases, disorders, and conditions, such as liver diseases. , for example, fatty liver, non-alcoholic fatty liver disease (NALFD), non-alcoholic steatohepatitis (NASH), non-alcoholic steatohepatitis associated with hepatic fibrosis, non-alcoholic steatohepatitis associated with liver cirrhosis, and non-alcoholic steatohepatitis associated with liver cirrhosis and hepatocellular carcinoma.

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

The invention discloses a phospholipase D mutant with improved thermal stability and a preparation method thereof. The amino acid sequence of the phospholipase D mutant is as shown in SEQ ID NO.4; according to the phospholipase D mutant A426P with improved thermal stability, the mutant is incubated for 30 min at 55 DEG C and 60 DEG C respectively, the residual enzyme activity is 85% and 58% respectively, the activity of wild-type enzyme is only 56% and 7% under the same treatment condition, the thermal stability of the mutant enzyme is remarkably improved, and the phospholipase D mutant A426P can be used as a phospholipase D mutant. The problem that phospholipase D is not ideal in thermal stability in biological catalytic reaction is solved.

The invention discloses a leontopodium alpinum callus extract as well as a preparation method and application thereof, and belongs to the technical field of plant active ingredient extraction. The leontopodium alpinum callus extract is prepared by the following steps: mixing leontopodium alpinum callus freeze-dried powder with a buffer solution, adding cellulase, pectinase and beta-glucanase, uniformly mixing, reacting to obtain primary enzymolysis slurry, adjusting the primary enzymolysis slurry to be neutral, adding phospholipase A2, stirring, reacting to obtain deep enzymatic hydrolysate, filtering, washing, and drying to obtain the leontopodium alpinum callus extract. Transferring into an extraction kettle, adding an entrainer, and extracting to obtain a leontopodium alpinum extract; dissolving soybean phospholipid and cholesterol in ethanol to obtain an organic phase; dispersing the leontopodium alpinum extract in a buffer solution to obtain an active matter water phase; and injecting the organic phase into the active matter water phase, stirring, carrying out reduced pressure distillation, and homogenizing. The preparation method provided by the invention can efficiently and completely obtain the active component, and the nano-liposome formed by phospholipid molecules can effectively improve the stability of the active component.

Certain one or more embodiments of the present disclosure are directed, inter alia, to recombinant (genetically modified) filamentous fungal cells (strains) that produce proteins of interest, methods and compositions for designing and constructing modified filamentous fungal cells that produce proteins of interest, for endogenous and / or heterologous proteins of interest (in modified filamentous fungal cells). The present invention relates to methods and compositions for expression / production / secretion / recovery and the like of recombinant filamentous fungi (e.g., phytase, lipase, glucoamylase, phospholipase, esterase, cellulase, hemicellulase, xylanase and the like), methods and compositions for the production of proteins of interest in recombinant filamentous fungi, and methods and compositions for the production of proteins of interest in recombinant filamentous fungi. Wherein the proteins produced and secreted into and / or recovered from the bouillon have a homogeneous and consistent N-linked glycosylation pattern, and / or a (unwanted) reduction in saccharification of one or more proteins of interest, and the like.

The present invention relates to the field of food processing technology, and discloses a fish oil and a refining method thereof. The method comprises: (1) first mixing crude fish oil with water, a complex enzyme, and a chelating agent to obtain a mixed material; the complex enzyme is a combination of protease, phospholipase, and lipase; (2) sequentially separating and washing the mixed material to obtain an intermediate material I; (3) sequentially subjecting the intermediate material I to a first adsorption decolorization, a second adsorption decolorization, and a third adsorption decolorization to obtain an intermediate material II; the adsorption material I in the first adsorption decolorization comprises silica gel; the adsorption material II in the second adsorption decolorization is bleaching clay; and the adsorption material III in the third adsorption decolorization is activated carbon; (4) deodorizing the intermediate material II to obtain a fish oil product. The method provided by the present invention can effectively remove impurities in crude fish oil, retain a high rate of beneficial accompaniments in fish oil, and effectively control the generation of harmful substances in the production process.

The invention relates to the technical field of bioengineering, in particular to a preparation process for extracting antihypertensive peptide from euphausia superba. The process comprises the following steps: adding a ferulic acid / arginine double-grafted pullulan compound in a preparation process, and breaking an emulsification interface by utilizing beta-cyclodextrin adsorption and phospholipase A2 cracking to obtain a soluble pretreatment product; meanwhile, short-time round-trip pH pulse is implemented in the incision enzyme hydrolysis process to activate potential enzyme cutting sites, finally, hydrophobic aggregation peptides are removed through weak acid rapid elution, and excision hydrolysis is performed to obtain a target product with the molecular weight of 0.5-3 kDa, so that dynamic induction and antioxidant steady-state dual control of hydrolysis reaction is realized; the generation proportion of short peptides and the ACE inhibition persistence are improved, and the flavor and the storage stability are remarkably improved. The method is controllable in technological process, high in repeatability and suitable for large-scale preparation of the euphausia superba protein functional peptide and development and application of antihypertensive food.

The present disclosure generally relates to a method for foaming and / or stabilizing foam in a milk or milk- based product, wherein a phospholipase is used to promote foam and / or its stabilization by acting on phospholipids and degrading the same and a use of a phospholipase-treated milk or milk-based product for creating milk foam.

The invention provides a lipoprotein sub-component phospholipid detection kit, and belongs to the field of in-vitro diagnostic reagents, and in particular, the lipoprotein sub-component phospholipid detection kit provided by the invention comprises a reaction reagent, the reaction reagent comprises a buffer solution, a surfactant, magnesium chloride, 4-aminoantipyrine, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3, 3, 4-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5- The preservative is prepared from 2, 5-dimethoxyaniline sodium salt, phospholipase D, choline oxidase, peroxidase, ascorbic acid oxidase, a protective agent and a third preservative. The lipoprotein subfraction phospholipid detection kit provided by the invention can be used for determining the content of lipoprotein subfraction phospholipid by using a VAP system, simultaneously realizes simultaneous detection of a plurality of lipoprotein subfraction phospholipid items for the first time, and has the advantages of high precision and high accuracy.

The invention relates to a phospholipase D mutant Lip-Gri / NQ, a plasmid, a recombinant bacterium and application of the phospholipase D mutant Lip-Gri / NQ and belongs to the field of enzyme engineering, the amino acid sequence of the phospholipase D mutant is shown as SEQ ID NO: 1, and the nucleotide sequence of a gene for coding the phospholipase D mutant Lip-Gri / NQ is shown as SEQ ID NO: 2. The invention also provides a recombinant plasmid and an engineering bacterium containing the gene of the recombinant plasmid phospholipase D mutant Lip-Gri / NQ, and application of the phospholipase D mutant Lip-Gri / NQ in catalyzing phospholipids to generate specific-configuration functional lipids including phosphatidylserine. According to the phospholipase D Lip-Gri disclosed by the invention, wild phospholipase D Lip-Gri from Streptomyces griseofuscus is transformed through site-directed mutagenesis, and the enzyme activity of the obtained mutant is remarkably improved and is 11 times that of the wild phospholipase D Lip-Gri.

Engineered phospholipase D mutants are described herein. Also described herein are methods of making engineered phospholipase D mutants. Additionally, methods of using engineered phospholipase D mutants are described.

The application belongs to the technical field of biological detection, and discloses a cardiovascular marker control article and a preparation method thereof. The cardiovascular marker control article comprises a serum matrix, antigens, a preservative and a stabilizer. The antigens include cardiac troponin I antigens, creatine kinase isoenzyme antigens, myoglobin antigens, amino-terminal brain natriuretic peptide precursor antigens, brain natriuretic peptide antigens, D-dimer antigens, heart-type fatty acid-binding protein antigens, myeloperoxidase antigens and lipoprotein phospholipase A2 antigens. The stabilizer includes at least one of D-trehalose, sucrose, glycine, inositol and polyethylene glycol. The cardiovascular marker control article can cover various cardiovascular disease markers, including heart failure, pulmonary embolism, vasculitis and atherosclerosis, myocardial injury, myocardial infarction and the like. The control article can be used as a third-party control article for indoor and inter-laboratory quality control of a cardiovascular disease screening and auxiliary diagnosis measurement system.

The invention belongs to the technical field of cosmetics. The invention relates to the field of cosmetics, in particular to a preparation method of camellia oil for cosmetics. The preparation method comprises the following specific preparation steps: adding phospholipase into camellia oil for degumming treatment to obtain degummed camellia oil; acidified montmorillonite and activated carbon are used as a compound decolorizing agent, and the degummed camellia oil is subjected to low-temperature decolorizing to obtain decolorized camellia oil; performing vacuum low-temperature deodorization on the decolored camellia oil to obtain deodorized camellia oil; the deodorized camellia oil is extracted with supercritical carbon dioxide, nitrogen is mixed into the supercritical carbon dioxide, the volume ratio of the nitrogen to the carbon dioxide is 1: (10-15), and the deeply detoxified camellia oil is obtained. Wherein dopamine can also be mixed in the supercritical carbon dioxide, and the dosage of the dopamine is 0.3-0.5% of the mass of the carbon dioxide.

This invention relates to nucleic acid aptamers for lipoprotein-associated phospholipase A2 (Lp-PLA2), wherein the nucleic acid aptamer is at least one of B76-2, B76-4, and B76-5, with sequences shown in SEQ ID NO.1, SEQ ID NO.2, and SEQ ID NO.3, respectively. The Lp-PLA2 aptamers provided by this invention fill a gap in the field of Lp-PLA2 aptamers and exhibit high affinity and specificity. Three aptamers with good affinity and specificity for Lp-PLA2 have been screened and verified. These aptamers can be formulated into molecular probes or used as detection reagents in methods or kits for detecting Lp-PLA2, improving accuracy. They can also be used to develop novel Lp-PLA2 biosensing methods for molecular identification, showing great application potential in the early warning of cardiovascular disease events.

The invention discloses a method for improving yield of anthocyanin extracted by an enzymic method, according to the method, phospholipase is added and used to act on a cell membrane structure, so that the extraction rate of the anthocyanin and the extraction rate of galactoside in the anthocyanin are obviously improved, and the extraction effect of the anthocyanin is greatly improved through a three-stage enzymolysis system. The total enzymolysis extraction time is within 4 h, and compared with 6-7 h of a traditional enzymatic extraction process, the production period is greatly shortened.

The application discloses application of phospholipase in promoting phosphorus removal in water body, and belongs to the technical field of water treatment. The phospholipase is added to a wetland or a constructed wetland system, the phospholipase catalyzes degradation of organic phosphorus into intermediates, and then shortens the time for conversion of the organic phosphorus into absorbable phosphorus, accelerates the conversion of the phosphorus into the absorbable phosphorus, improves the absorption efficiency of plants on the phosphorus, and thus improves the removal efficiency of the wetland or the constructed wetland system on the phosphorus in the water body.

Provided is a composition for preparing a medicament for treating diabetic peripheral neuropathy. The pharmaceutical composition comprises cobra venom phospholipase A2, or a combination of cobra venom phospholipase A2 and at least one additional drug for treating diabetic peripheral neuropathy. The diabetic peripheral neuropathy is characterized by a slowdown in motor nerve conduction velocity and / or sensory nerve conduction velocity of a patient, and / or hypoesthesia or loss of sensation in both lower limbs. The pharmaceutical composition can be used to ameliorate the clinical symptoms described above in patients with diabetic peripheral neuropathy, and belongs to the field of biopharmaceuticals.

To provide therapies for subjects suffering from NAFLD.SOLUTION: A double stranded ribonucleic acid (RNAi) agent for inhibiting expression of Patatin-Like Phospholipase Domain Containing 3 is used which comprises a sense strand and an antisense strand. The sense strand comprises at least 15 contiguous nucleotides differing by 3 or less nucleotides from a specific nucleotide sequence. The antisense strand comprises at least 15 contiguous nucleotides differing by 3 or less nucleotides from another specific nucleotide sequence.SELECTED DRAWING: Figure 1

To obtain a novel phospholipase D against choline-type plasmalogen.SOLUTION: Prepared is a phospholipase D comprising any of the described polypeptides: (1-1) a polypeptide having the amino acid sequence set forth in SEQ ID NO: 1; (1-2) a polypeptide having at least 85% identity with the amino acid sequence set forth in SEQ ID NO: 1, and having a hydrolytic activity to a phosphate ester bond in a choline-type plasmalogen molecule in which oleic acid is ester-bonded to the sn-2 position of a glycerol skeleton; and (1-3) a polypeptide in which one or several amino acids are added, substituted, deleted and / or inserted in the amino acid sequence set forth in SEQ ID NO: 1, and having a hydrolytic activity to a phosphate ester bond in a choline-type plasmalogen molecule.SELECTED DRAWING: Figure 3

The present invention belongs to the field of enzyme genetic engineering technology and specifically relates to a phospholipase D mutant, its preparation, and use. The phospholipase D gene from Streptomyces antibioticus was molecularly modified to mutate histidine at position 443 to asparagine and serine at position 199 to histidine, resulting in the phospholipase D mutant of the present invention, the amino acid sequence of which is shown in SEQ ID NO. 3. The catalytic activity of this mutant is significantly enhanced compared to that of wild-type phospholipase D. The phospholipase D mutant obtained by the present invention is suitable for catalyzing the synthesis of glycerophospholipids and also provides a reference for the synthesis of other functional phospholipids.

A platform for therapy provides a multi-target therapeutic molecule comprising an anti-CD3 antigen binding fragment of an anti-CD3 antibody linked with a linker to two or more auto-antigen(s). The auto-antigens include Dsg1, Dsg3, bullous pemphigoid antigen 180, bullous pemphigoid antigen 230, MuSK, anti-phospholipase A2 Receptor. A pharmaceutical composition comprises a disclosed multi-target therapeutic molecule is disclosed. Methods of treating diseases using the disclosed molecules are disclosed