5 results about "Lipolytic enzyme" patented technology

The invention discloses a compound enzyme preparation-containing nutritional feed for regulating and controlling intramuscular fat of black pigs and a preparation method of the compound enzyme preparation-containing nutritional feed, and relates to the technical field of livestock and poultry breeding feeds. The invention discloses a black pig skeletal muscle cell fat directional transport feed which is prepared from the following components in parts by weight: 850-920 parts of basal feed, 5-12 parts of fat directional deposition compound enzyme group, 25-40 parts of amino acid-enzymatic synthesis substrate combination and 2-6 parts of lipolytic enzyme specific inhibitor. According to the black pig skeletal muscle cell fat directional transport feed, fat precursor directional transport is realized through the compounded fat directional deposition compound enzyme group, a fat synthesis pathway targeting black pig skeletal muscle cells and lipoprotein lipase; acetyl-coenzyme A carboxylase and fatty acid synthetase catalyze fat synthesis step by step, only intramuscular fat generation pathways are activated, subcutaneous and abdominal fat deposition paths are blocked, the technical defects that fat deposition of an existing single enzyme preparation is non-directional, and the body fat rate is caused to be abnormally increased are overcome, meanwhile, an exclusive amino acid-enzymatic synthesis substrate combination is matched, and the body fat synthesis efficiency is improved. Sufficient adaptive substrates are supplied to enzyme group catalytic reaction, and the problem that a single enzyme preparation is insufficient in catalytic efficiency is solved.

The present invention relates to a composition for producing chitin and a method for producing chitin, comprising a Bacillus subtilis EG1 strain or a culture solution thereof as an active ingredient. Since the Bacillus subtilis EG1 strain or a culture solution thereof according to the present invention has proteolytic and lipolytic enzyme activities, it can be effectively utilized for producing chitin from a mealworm larva substrate.

The invention provides a method for synthesizing dehydroepiandrosterone through chemical-multienzyme coupling catalysis, and belongs to the technical field of pharmaceutical preparations with specific therapeutic activity. The method comprises the following steps: by taking 4-androstenedione as a substrate, carrying out esterification to synthesize 3-acetoxyandrostane-3, 5-diene-17-ketone; adding a lipolytic enzyme, and catalyzing the 3-acetoxyandrostane-3, 5-diene-17-ketone to synthesize the 5-androstenedione; ketoreductase and glucose dehydrogenase are added, and 5-androstenedione is catalyzed through double-enzyme coupling to synthesize DHEA. The method provided by the invention can realize efficient synthesis of DHEA.

The invention provides a method and system for the enzymatic transesterification of fatty acids or fatty acid derived products to produce lubricant esters. The process involves a reaction mixture containing a fatty acid or fatty acid-derived substrate, an alcohol and a lipolytic enzyme catalyst. A reactive column with a porous frit is employed through which air is bubbled into a mixture of fatty acid derivatives, alcohol and an immobilized lipase catalyst. The air bubbles promote mixing and the subsequent stripping of volatile by-products, thereby increasing ester formation. This process can be carried out at ambient temperature, without the need for external heating or microwave irradiation. The method achieves greater that 99 mol % conversion to ester products at lower energy when compared to conventional thermochemical transesterification.

The application discloses a soil staphylococcus with high-temperature resistance and smoking resistance, and application thereof, and belongs to the technical field of microbial application and fermented meat products. The soil staphylococcus has a preservation number of CGMCC No. 34994, is named XUCSB 058, has nitrate reductase, catalase, proteolytic enzyme and lipolytic enzyme, can produce acid by utilizing glucose, and has the characteristics of acid resistance, salt resistance, nitrite resistance, low-temperature resistance, non-hemolysis, non-stick production, non-gas production, non-biogenic amine production, non-NH3 production, non-H2S production and the like. The soil staphylococcus has the characteristics of high-temperature resistance and smoking resistance, can promote the persistent formation of color and flavor of smoking fermented meat (such as bacon, sausage, smoked ham and the like), and has the potential of being safely used in the production of various smoking fermented meat (such as bacon, sausage, smoked ham and the like).