172 results about "Lipolytic enzyme" patented technology

There is provided an enzymatic detergent composition which comprises: (a) surfactant; (b) 10-20,000 LU per gram of the detergent composition of a lipolytic enzyme obtainable from Humicola lanuginosa, Pseudomonas pseudoalcaligenes, Rhizomucor miehei and (c) a non-cross-bridged polydentate N-donor ligand capable of forming a complex with a transition metal, wherein said complex is capable of catalysing the bleaching of stains on fabrics by means of atmospheric oxygen.

Lipolytic enzymes with high activity at alkaline pH in the absence of Ca++ can be obtained from filamentous fungi of the genera Gliocladium, Verticillium and Trichophaea and that the lipolytic enzymes are effective for improving the effect of detergents. The lipolytic enzymes have a good washing performance, as expressed by the hydrolysis of oil on textile swatches. The amino acid sequences of the lipolytic enzymes are highly homologous.

The invention encompasses the use of a lipolytic enzyme obtainable from one of the following genera: Streptomyces, Corynebacterium and Thermobifida in various methods and uses, wherein said lipolytic enzyme is capable of hydrolysing a glycolipid or a phospholipid or transferring an acyl group from a glycolipid or phospholipids to an acyl acceptor. Preferably, the lipolytic enzyme for use in these methods and uses comprises an amino acid sequence as shown in any one of SEQ ID NOs: 4, 5, 7, 8, 12, 14 or 16 or an amino acid sequence having at least 70% identity therewith or comprises a nucleotide sequence shown as SEQ ID NO: 3, 6, 9, 13, 15 or 17 or a nucleotide sequence which has at least 70% identity therewith. The present invention also relates to a lipolytic enzyme capable of hydrolysing at least a galactolipid or capable of transferring an acyl group from a galactolipid to one or more acyl acceptor substrates, wherein the enzyme is obtainable from Streptomyces species and includes a lipolytic enzyme comprising an amino acid sequence as shown in SEQ ID NO: 4 or an amino acid sequence which has at least 60% identity thereto. The enzyme may be encoded by a nucleic acid selected from the group consisting of: a) a nucleic acid comprising a nucleotide sequence show in SEQ ID NO: 3; b) a nucleic acid which is related to the nucleotide sequence of SEQ ID NO: 3 by the degeneration of the genetic code; and c) a nucleic acid comprising a nucleotide sequence which has at least 70% identity with the nucleotide sequence shown in SEQ ID NO: 3.

The inventor has found that enzymatic treatment of crustaceans with lipolytic enzymes promotes the extraction of coloured pigment. Accordingly, the invention relates to a method for recovering coloured pigment, e.g. astaxanthin, and / or chitin or chitosan and / or lysolecithins from crustaceans. Lipolytic enzyme(s) may e.g. be lipase or phospholipase. The lipolytic enzyme treated crustacean material may be subjected to additional process steps, including treatment with non-lipolytic enzymes such as e.g. protease. The process of the invention provides several suitable products and several uses of such products. Furthermore, the invention also relates to the cosmetic and consumable products (feed and food (including beverages) products and additives produced therefrom.

A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

The present invention provides a liquid detergent composition, comprising: (a) a cleaning effective amount of an enzyme preferably selected from a proteolytic enzyme, an amylolytic enzyme, a lipolytic enzyme, a cellulolytic enzyme and mixtures thereof; (b) from 0.001 to 3% by weight of a perfume composition, not containing a perfume component selected from the group consisting of saturated and unsaturated linear aldehydes, lilial, cyclal c, vanillin, citral, cinnamic aldehyde, pulegone, terpinolene, gamma terpinene, alpha methylionone; (c) from 0.002 to 1% by weight of an antioxidant; and (d) a fatty acid soap in an amount of at most 4% by weight. Said detergent composition was found to have favourable storage stability characteristics.

The invention relates to a process for preparing diglyceride grease comprising the steps of, (1) adsorbing the glycerine onto adsorption material, (2) mixing the adsorption material obtained in step (1) and glycerine with triglyceride, using immobilization lipolytic enzyme as catalyst for catalyzing the reaction between triglyceride and glycerine at 20-90 deg. C, (3) separation by removing immobilization lipolytic enzyme and glycerine adsorption material from the outcome yield of (2), separating aliphatic acid to obtain diglyceride grease.

The invention discloses a method of catalyzing and synthesizing alpha-glyceryl linolenate with immobilized lipolytic enzyme in the manufacturing method field of a anti-cancer drug material, which comprises the following steps: making immobilized enzyme carrier including hole enlarge of dielectric hole material and the amido modification; making immobilized lipolytic enzyme, synthesizing alpha-glyceryl linolenate with immobilized enzyme in organic medium. The invention provides a good steady, and SBA-15 still shows regular hexagonal structure after modification, a uniform pore path, a big aperture and meets the need of synthesizing alpha-glyceryl linolenate; improvement of the lipase proper temperature improves the productivity; the product is provided with a light yellow color, a good quality, a good curative effect, enhancing heat stability.

The invention belongs to a preparation method of a face-beautifying weight-reducing enzyme, and particularly relates to a preparation method of a face-beautifying weight-reducing fruit enzyme. The preparation method comprises the following steps: preparing 40 parts of fruits rich in carbohydrate, 20 parts of fruits rich in vitamin C and 40 parts of fruits rich in lipolytic enzyme; washing the fruits with clean water and adding ozone to sterilize the fruits for 30 minutes, and naturally drying the fruits in air; crushing the air-dried fruits into pulps by use of a low-rotating-speed crusher; and subsequently filling the pulps with 5-7 parts of wine yeast powder, simultaneously adding 8 parts of white sugar, and then fermenting the pulps in a fermentation tank. The prepared fruit enzyme original solution has good face beautifying and weight reducing effects and also is free of side effects. In addition, the preparation method disclosed by the invention is not high in cost, simple and easy to operate, and the prepared face-beautifying weight-reducing fruit enzyme has relatively high nutritional values and is suitable for being drunk by a wide range of people.

A preparation technique of chitosan microspheres immobilization lipase comprises the steps that: (1) lipase from candida SP is dissolved in phosphate buffer solution by taking chitosan microspheres as an immobilization carrier and adopting glutaraldehyde cross-linking, lipase A is obtained. (2) candida SP lipase solution of 0.70-0.80mg / mL prepared by phosphate buffer solution b with a pH value of 6.5-7.5 is added with immobilization lipase A (final concentration is 0.005-0.0055g / ml) and after being stirred, ethyl (3-dimethylamino propyl) carbodiimide hydrochloride solution is added, the mixture is then stirred to be fully immobilized, chitosan microspheres immobilization lipase B is obtained after the treatment of the mixture. The chitosan microspheres immobilization lipase prepared by the preparation technology of the invention improves apoenzyme combination rate and activity recovery, has powerful immobilization lipase activity and specific activity and the immobilization lipase can be recycled for six times.

The method for extracting dioscin and diosgenin includes the following steps: pulverizing rhizome of Chinese yam plant by adding water, fermentation by using lipolytic enzyme, separating cellulose and lignin, recovering supernatant, suspension and starch, mixing recovered liquors and filtering residue, boiling and filtering, repeating for several times, filtering residue for stand-by, combining filtrates and concentrating, using ethyl alcohol to make reflux extraction, cooling and filtering, decompressing and concentrating filtrate, adsorbing, evaporating, ethyl alcohol extracting, adding ethyl ether, cooling to room temp. and separating out tetrasaccharide saponin, recovering ethyl alcohol and ethyl ether from supernatant, precipitating out saponin, drying, adding water in starch and filter residue, regulating pH, adding alpha-amylase.

The invention discloses a method for producing meat flavor by using enzyme hydrolysis adipose as raw material which is oxygenized and thermo-cracked, the method which uses adipose as raw material comprises steps as follows: by utilizing a preferred lipolytic enzyme, part of triglyceride in the adipose is hydrolyzed to obtain free fatty acid via biological transformation under specific condition, and then taking the product of the enzyme hydrolysis adipose as reactant, the oxidation and thermal cracking reaction are carried through to obtain natural meat flavor. The invention has the advantages of natural aroma, simple processes and low cost.

The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper. The method includes mixing water, alcohol, triglyceride and / or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.

The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.

The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.

Compositions and methods to treat biofilms are disclosed based on the discovery of the role of the disaccharide trehalose in microbial biofilm development. In various embodiments to treat body-borne biofilms systemically and locally, the method includes administering trehalase, the enzyme which degrades trehalose, in combination with other saccharidases for an exposition time sufficient to adequately degrade the biofilm gel matrix at the site of the biofilm. The method also includes administering a combination of other enzymes such as proteolytic, fibrinolytic, and lipolytic enzymes to break down proteins and lipids present in the biofilm, and administering antimicrobials for the specific type(s) of infectious pathogen(s) underlying the biofilm. Additionally, methods are disclosed to address degradation of biofilms on medical device surfaces and biofilms present in industrial settings.

The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well asfunctional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.

Provided are a porous structure for forming anti-fingerprint coating capable of providing a self-cleaning function to a surface of a substrate, a method of forming anti-fingerprint coating using the same, an anti-fingerprint coated substrate prepared by the same method, and a product including the same. When the porous structure including a lipolytic enzyme is formed on the surface of the substrate, contaminants decomposed by an enzyme are absorbed into a pore, and thus anti-fingerprint coating may be more effectively performed to remove detectable contamination from a surface of the substrate. As a result, contamination by fingerprints on the surface of a display device, the appearance of an electronic device, or building materials can be effectively reduced.

A simplified preparation technology of rice oil belongs to the grain oil processing field. The simplified preparation technology provided by the invention comprises the following steps of: firstly, rice bran sold in the market is taken as a raw material and undergoes a microwave irradiation pretreatment to reduce the activity of lipolytic enzyme ; secondly, the rice bran undergoes the supercritical CO2 fractional extraction to obtain crude rice bran oil, followed by refining crude rice bran oil produced during the later period with the use of diethanolamine; thirdly, separating to obtain oil phase, washing and drying to obtain the finished product rice oil. The integrated application of microwave irradiation, supercritical fluid fractional extraction and diethanolamine refining method can simplify traditional preparation technology of rice oil and reduce the loss of neutral oil and oryzanol in the refining process. The rice oil product prepared by the technology has a unique fragrance of baked food with clear, transparent and light yellow grease; after the integrated processing, the acid value can be reduced below 1.0mgKOH / g, the content of phosphor is lower than 5mg / kg, the content of oryzanol reaches over 1.70%, and the chromaticity meets the national quality standard of leaching finished rice oil products.

The present invention provides lipolytic enzyme variants. Specifically, the present invention provides lipolytic enzyme variants having one or more modifications as compared to a parent lipolytic enzyme having at least one improved property. In addition, the present invention provides compositions comprising a lipolytic enzyme variant of the invention. The present invention also provides methods of cleaning using compositions comprising a lipolytic enzyme variant of the invention.

A process for producing a glyceride product which is enriched in monounsaturated fatty acids relative to the starting glyceride comprising the steps: (a) alcoholysis of triglycerides employing lipolytic enzymes selective for saturated fatty acids and / or lipolytic enzymes selective for the 1-position, the 3-position or both positions in a glyceride; and (b) separation of fraction A which is enriched in saturated fatty acid esters from fraction B which is enriched in monounsaturated glycerides.

The invention discloses instant noodle made of Tibetan highland barley. The instant noodle takes modified highland barley flour and modified starch as main raw materials. A preparation method comprises the steps of curing, tabletting, standing, slitting, puffing to form, and drying to obtain the non-fried instant noodle cake made from the highland barley, wherein the mass ratio of the modified highland barley flour to the modified starch is (80-95):5; and the modified highland barley flour is prepared from the following components in percentage by mass: 35-45 percent of water, 5-10 percent of protease, 4-8 percent of pentosanase, 4-8 percent of lipolytic enzyme, 1-1.5 percent of salt and the balance of highland barley powder, which are subjected to enzyme digestion. According to the invention, highland barley flour is modified, so that the mucedin of the flour is improved to prepare the flour for processing the noodle; in cooperation with a processing technique of non-fried instant noodle and with adoption of modern instant noodle preparation equipment, the mouth feel of the instant noodle made from the highland barley has the palatable degree of instant noodle made from wheat, and the instant food with unique flavor and comprehensive nutrition is prepared. The preparation method is high in forming rate and good in palatable degree.

The present invention to newly identified polynucleotide sequences comprising genes that encode novel lipolytic enzymes LIP01-LIP03. The LIP01 enzyme may be isolated from Magnaporthe grisae, the LIP02 and LIP03 may be obtained by mutating the polynucleotide sequence encoding for LIP01. The invention features the full length coding sequence of the novel gene, which is suitable for expression in a suitable host cell as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods of using these proteins in industrial processes, for example in baking industry, vegetable oil degumming, production or modification of food emulsifiers and the production of glucose from wheat gluten.

The present invention relates to the field of methods for purifying fatty acid ethyl esters. According to the present invention, a method for obtaining a ω3 fatty acid ethyl ester, such as EPA and DHA, each as a high purity product at a high yield is provided. In the method according to the present invention, a raw material fat including EPA and DHA is treated with a lipolytic enzyme and ethyl-esterification is performed as needed; the treated substance is fractionated into a glyceride fraction and a free fatty acid fraction; a fraction comprising more EPA ester and a fraction comprising DHA ester are obtained from the respective fractions; the fraction comprising more EPA ester is purified to prepare a high-purity EPA ester; and the fraction comprising more DHA ester is purified to prepare a high-purity DHA ester.

A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipid:triglyceride activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolyzing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID NO: 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

The invention relates to a method for preparing hawthorn dry red wine, comprising the following steps of taking hawthorn as raw materials, smashing the hawthorn, mixing sugar, mixing natural dry yeast and additives, loading the mixture in a container filled with hot water, fermenting for 5 to 50 days under the condition of 10 to 30 DEG C, carrying out aging fermentation for 30 to 90 days, then chilling for 5 to 30 days under the temperature of 5 DEG C below zero to 8 DEG C, clarifying, filtering, freezing and sterilizing , checking and filling to prepare the hawthorn dry red wine which has the sugar degree of less than 4 degrees, the alcoholic degree of 12 to 15 degrees and good taste, is acidulous, sweet (slightly sweet), bitter (slightly bitter), astringent and crystal clear and in the color of gem red. The brewing process of the hawthorn dry red wine is the fermentation process of transforming the sugar to the alcohol, the processes of leaching nutrients of amino acid, hawthorn acid, flavone, vitamin C, trace elements, and the like in the hawthorn are carried out at the same time, smashed red hawthorn is transformed to the hawthorn dry red wine, and organic acid, vitamins, flavonoids, the trace elements, lipolytic enzyme and the like in haw apples are transferred to the hawthorn dry red wine.

A green detergent relates to a non-toxic harmless environment friendly tableware detergent and belongs to the field of detergents. Limonene adopted in the green detergent is a natural defouling and deoiling substance which is obtained by distilling and refining lemon fruit peels, has excellent deoiling and defouling capacity, can be biodegradable and is pollution-free after mixed with water in any proportion; simultaneously for components adopted by the green detergent, roxburgh rose roots are the roots of roxburgh roses belonging to rosaceous plants, have the functions of tonifying spleen to promote digestion and astringing to arrest diarrhea, are rich in tannins and can dissolve dirt and clean articles; the fruits of large-fruited Chinese hawthorn belonging to large-fruited Chinese hawthorn rosaceous plants can relieve dyspepsia and disperse stasis to relive pain, are rich in lipolytic enzyme and have the functions of degrading lipid and activating scale; daghestan sweet clover is the whole plant of the daghestan sweet clover belonging to leguminous plants, has the functions of resolving dampness with aromatics and preventing sunstroke, epidemic and dysentery, is rich in fragrant grass elements and can release pleasant fragrance to make people have good mood and emotions; stems of broad beans are rich in tannin and have the degreasing efficacy; banana stalks have strong deoiling effect; and spinaches contain lots of iron element and are of great benefit for human bodies.

Compositions and methods to treat biofilms are disclosed based on the discovery of the role of the disaccharide trehalose in microbial biofilm development. In various embodiments to treat body-borne biofilms systemically and locally, the method includes administering trehalase, the enzyme which degrades trehalose, in combination with other saccharidases for an exposition time sufficient to adequately degrade the biofilm gel matrix at the site of the biofilm. The method also includes administering a combination of other enzymes such as proteolytic, fibrinolytic, and lipolytic enzymes to break down proteins and lipids present in the biofilm, and administering antimicrobials for the specific type(s) of infectious pathogen(s) underlying the biofilm. Additionally, methods are disclosed to address degradation of biofilms on medical device surfaces and biofilms present in industrial settings.