12 results about "Lipolytic enzyme" patented technology

The invention discloses a compound enzyme preparation-containing nutritional feed for regulating and controlling intramuscular fat of black pigs and a preparation method of the compound enzyme preparation-containing nutritional feed, and relates to the technical field of livestock and poultry breeding feeds. The invention discloses a black pig skeletal muscle cell fat directional transport feed which is prepared from the following components in parts by weight: 850-920 parts of basal feed, 5-12 parts of fat directional deposition compound enzyme group, 25-40 parts of amino acid-enzymatic synthesis substrate combination and 2-6 parts of lipolytic enzyme specific inhibitor. According to the black pig skeletal muscle cell fat directional transport feed, fat precursor directional transport is realized through the compounded fat directional deposition compound enzyme group, a fat synthesis pathway targeting black pig skeletal muscle cells and lipoprotein lipase; acetyl-coenzyme A carboxylase and fatty acid synthetase catalyze fat synthesis step by step, only intramuscular fat generation pathways are activated, subcutaneous and abdominal fat deposition paths are blocked, the technical defects that fat deposition of an existing single enzyme preparation is non-directional, and the body fat rate is caused to be abnormally increased are overcome, meanwhile, an exclusive amino acid-enzymatic synthesis substrate combination is matched, and the body fat synthesis efficiency is improved. Sufficient adaptive substrates are supplied to enzyme group catalytic reaction, and the problem that a single enzyme preparation is insufficient in catalytic efficiency is solved.

The invention discloses lipolytic enzyme MfLPE and application of the lipolytic enzyme MfLPE to synthesis of ester spices, and belongs to the technical fields of enzyme engineering, new enzyme resource mining, biological catalysis and green chemical engineering. According to the method disclosed by the invention, lipolytic enzyme MfLPE obtained by heterologous expression and purification is used as a biocatalyst, and cheap and easily available acyl donors (methyl p-nitrobenzoate, methyl p-nitrobenzene butyrate or methyl p-nitrobenzene caprylate) and common aromatic alcohols (benzyl alcohol and phenethyl alcohol) are subjected to catalytic reaction in pure water or a small amount of organic solvent; typical ester perfumes such as benzyl acetate, phenethyl acetate and phenethyl caprylate can be efficiently synthesized, the conversion rate reaches 75% or above, the product selectivity is high, the technological conditions are mild, environment friendliness is achieved, amplification to a continuous flow reaction is easy, and organic solvent dependence and production cost are remarkably reduced. The invention provides a new enzyme source and process scheme for green biosynthesis of ester spices, and has a good industrial application prospect.

The present invention relates to a composition for producing chitin and a method for producing chitin, comprising a Bacillus subtilis EG1 strain or a culture solution thereof as an active ingredient. Since the Bacillus subtilis EG1 strain or a culture solution thereof according to the present invention has proteolytic and lipolytic enzyme activities, it can be effectively utilized for producing chitin from a mealworm larva substrate.

The invention provides a method for reducing free fatty acid level in biodiesel / fatty acid alkyl ester. The method comprises providing a composition comprising a fatty acid alkyl ester, a free fatty acid and / or a fatty acid feedstock, reacting the free fatty acid and / or the fatty acid feedstock with an alcohol in the presence of one or more liquid lipolytic enzymes to produce a fatty acid alkyl ester; and / or reacting the free fatty acid and / or the fatty acid feedstock with an alcohol in the presence of one or more non-enzymatic catalysts to produce a fatty acid alkyl ester.

The invention provides a method for synthesizing dehydroepiandrosterone through chemical-multienzyme coupling catalysis, and belongs to the technical field of pharmaceutical preparations with specific therapeutic activity. The method comprises the following steps: by taking 4-androstenedione as a substrate, carrying out esterification to synthesize 3-acetoxyandrostane-3, 5-diene-17-ketone; adding a lipolytic enzyme, and catalyzing the 3-acetoxyandrostane-3, 5-diene-17-ketone to synthesize the 5-androstenedione; ketoreductase and glucose dehydrogenase are added, and 5-androstenedione is catalyzed through double-enzyme coupling to synthesize DHEA. The method provided by the invention can realize efficient synthesis of DHEA.

The present invention relates to a wild-type, non-engineered, microbial lipolytic enzyme with a higher specificity for short-chain fatty acids than for medium to long fatty acids, specially long fatty acids. This invention further relates to a process for preparing a food product, and to the use of the wild-type, non-engineered, microbial lipolytic enzyme.

The invention relates to the field of bakery ingredients. More specifically, the invention relates to a variant of a parent polypeptide, wherein the difference between the variant polypeptide and the parent polypeptide is that a KEX2 protease cleavage site which is present in the parent polypeptide, is absent in the variant polypeptide. The invention further relates to a process for preparing a dough wherein a variant polypeptide as disclosed herein is used and baked product prepared from the dough.

The invention relates to the technical field of fat-reducing products, in particular to a soybean peptide fat-reducing composition as well as a preparation method and application thereof. The soybean peptide fat-reducing composition is prepared from the following raw materials in parts by mass: 55 to 65 parts of soybean peptide, 18 to 30 parts of plant peptide, 10 to 25 parts of dietary fiber, 1.8 to 3.5 parts of green tea extract, 3 to 10 parts of erythritol, 7 to 18 parts of diglyceride oil, 1.5 to 4 parts of citrus polyphenol and 0.8 to 1 part of auxiliary materials. After the soybean peptide and the plant peptide are compounded, the lipid lowering rate is increased by activating lipolytic enzyme (HSL), enhancing mitochondrial fatty acid oxidation (AMPK), blocking fat synthesis (FAS), improving the lipolytic efficiency and the like.

The invention provides a method and system for the enzymatic transesterification of fatty acids or fatty acid derived products to produce lubricant esters. The process involves a reaction mixture containing a fatty acid or fatty acid-derived substrate, an alcohol and a lipolytic enzyme catalyst. A reactive column with a porous frit is employed through which air is bubbled into a mixture of fatty acid derivatives, alcohol and an immobilized lipase catalyst. The air bubbles promote mixing and the subsequent stripping of volatile by-products, thereby increasing ester formation. This process can be carried out at ambient temperature, without the need for external heating or microwave irradiation. The method achieves greater that 99 mol % conversion to ester products at lower energy when compared to conventional thermochemical transesterification.

The application discloses a soil staphylococcus with high-temperature resistance and smoking resistance, and application thereof, and belongs to the technical field of microbial application and fermented meat products. The soil staphylococcus has a preservation number of CGMCC No. 34994, is named XUCSB 058, has nitrate reductase, catalase, proteolytic enzyme and lipolytic enzyme, can produce acid by utilizing glucose, and has the characteristics of acid resistance, salt resistance, nitrite resistance, low-temperature resistance, non-hemolysis, non-stick production, non-gas production, non-biogenic amine production, non-NH3 production, non-H2S production and the like. The soil staphylococcus has the characteristics of high-temperature resistance and smoking resistance, can promote the persistent formation of color and flavor of smoking fermented meat (such as bacon, sausage, smoked ham and the like), and has the potential of being safely used in the production of various smoking fermented meat (such as bacon, sausage, smoked ham and the like).

To provide a topical composition for skin that can suppress reduction in activity of proteolytic enzymes and lipolytic enzymes.SOLUTION: A topical composition for skin comprising: (A) a proteolytic enzyme and / or a lipolytic enzyme, and (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium robertianum extract, hyaluronic acid, and a salt thereof.SELECTED DRAWING: None

The invention discloses a compound enzyme preparation capable of accelerating degradation of organic garbage and a preparation method of the compound enzyme preparation, and belongs to the technical field of garbage degradation. The compound enzyme preparation is prepared from the following components in parts by weight: 50 parts of purified water, 10 to 20 parts of modified fat hydrolase, 10 to 20 parts of immobilized high-activity pectinase, 10 to 30 parts of cellulose hydrolase, 10 to 20 parts of hypersensitive protein compound enzyme, 5 to 15 parts of amylase, 5 to 10 parts of compound microorganisms, 5 to 10 parts of glycerol, 1 to 5 parts of brown sugar, 0.5 to 1 part of Tween-80 and 0.1 to 0.3 part of trace elements, the modified lipolytic enzyme is obtained by modifying lipolytic enzyme with glutaraldehyde and glutamine transaminase; the immobilized high-activity pectinase is obtained by immobilizing high-activity pectinase through silica gel. The compound enzyme preparation disclosed by the invention can accelerate the degradation of organic garbage.