72 results about "Trehalase" patented technology

The invention relates to a separation and purification method of trehalose, which comprises the following steps: (1) carrying out enzymolysis on a trehalase reaction solution prepared by a trehalose synthase conversion process, and filtering to obtain a trehalose crude solution; (2) decolorizing the trehalose crude solution through activated carbon, and filtering to obtain a mixed solution; (3) passing the mixed solution through an ion exchange resin to obtain a glucose-trehalose sugar solution; and (4) concentrating the glucose-trehalose sugar solution, and separating by continuous chromatography with a simulated moving bed to obtain a trehalose solution and a glucose solution. The trehalase reaction solution generated by converting maltose by trehalose synthase is used as the raw material, the maltose which is isomeric with the trehalose is converted by an enzyme process, and impurity glucose, remaining maltose and impurities, such as proteins, pigments, metallic ions and the like, are removed to obtain the high-purity trehalose.

A burn medicine delivery system for treating burn is prepared from trehalase, hyaluronic acid, and medicinal additive.

The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more trehalases are present in the fermentation medium.

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and / or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and / or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

The invention provides an entomopathogenic fungi transgenic strain which contains a homologous or heterologous acidic trehalase constitutive exogenous gene. The preparation method of the entomopathogenic fungi transgenic strain comprises the following steps: firstly building an acidic trehalase full-length cDNA gene constitutive expression vector; establishing a submerged conidia transformation system; and finally selectively screening to obtain the entomopathogenic fungi transgenic strain. The invention also provides application of Entomopathogenic fungi acidic trehalase transgenic strain in preparing fungal pesticide. The entomopathogenic fungi transgenic strain has high efficiency of expressing trehalase; and the preparation method has the advantages of easy operation, good repeatability and good reproducibility. The entomopathogenic fungi transgenic strain can be applied to preparing the fungal pesticide. When the fungal pesticide acts on insects, through the overexpression of the acidic trehalase gene via the transgenic strain, the activity of acidic trehalase in an insect host is improved, utilization of the trehalase is accelerated and growth of entomopathogenic fungi in the insect host is promoted, thus effectively improving the insecticidal activity of the fungal pesticide such as metarhizium anisopliae, beauveria bassiana and the like.

The invention discloses a botanical insect trehalase inhibitor and the application thereof. The inhibitor comprises castanospermine which can be obtained by screening botanical compounds and used for manufacturing trehalase-targeted pesticides or active lead compounds. The inhibitor has good effect on inhibiting trehalase in cotton bollworms and other insects.

The invention belongs to the technical field of agricultural genes and in particular relates to acidic trehalase TreA sourced from a fungus and a gene and application thereof. The amino acid sequenceof the trehalase TreA is as shown in SEQ ID NO.1 or SEQ ID NO.2. The invention provides a novel trehalase gene. The trehalase encoded by the trehalase gene has high properties and can be applied to industries of insect control, foot, starch sugar, medicines and the like.

The present invention relates to genetically engineered yeasts having a heterologous trehalase gene and fermentation processes for using such yeasts. The yeasts can express trehalase in a quantity sufficient to convert significant amounts of trehalose to glucose, thereby improving the yield of the product in a fermentation, and / or reducing or eliminating the need to add exogenous trehalase to the fermentation. The yeasts can also include other heterologous genes for expressing enzymes useful for improving yield and / or for reducing or eliminating the need to add exogenous enzymes to the fermentation.

This invention relates to isolated nucleic acid fragments encoding trehalose metabolism enzymes, more specifically, alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the alpha, alpha-trehalase, alpha, alpha-trehalose-phosphate synthase or trehalose-6-phosphate phosphatase in a transformed host cell.

The invention provides transgenic plants that exhibit increased resistance to nematode infection by virtue of overexpression of a gene that encodes trehalase in nematode-induced syncytia. Expression vectors comprising trehalase-encoding polynucleotides and methods of employing such vectors to increase nematode resistance of plants are also provided.

The invention provides a process for producing trehalose in plant cells capable of producing trehalase by growing plant cells having the genetic information required for the production of trehalose and trehalase, or cultivating a plant or a part thereof comprising such plant cells, characterized in that said plant cells are grown, or said plant or a part thereof, is cultivated in the presence of a trehalase inhibitor.