The invention relates to a preparation method of a double-source
amino acid-containing organic water-soluble
fertilizer and the prepared water-soluble
fertilizer, and belongs to the technical field of agricultural bio-fertilizers. The invention aims to solve the problems of unbalanced
amino acid spectrum, easy generation of peculiar smell or heavy
metal residue and low
nutrient release efficiency when a single
raw material is adopted for preparation. The preparation method comprises the following steps: hydrolyzing an animal-derived
protein raw material with an acid solution, and adding embedded slow-release
vitamin C; the method comprises the following steps: carrying out gradient heating on a
plant source
protein raw material by using 1.0-1.5 mol / L
potassium hydroxide at 90-100 DEG C, and carrying out ultrasonic-assisted
hydrolysis for 3-5 hours; mixing the two hydrolysates according to the volume ratio of 3: 7, and regulating the pH to 6.5-7.0 in stages; adding a compound
enzyme preparation consisting of
neutral protease,
papain and
trehalase in a
mass ratio of 3: 2: 1 into the neutralized
mixed solution, and carrying out enzymolysis at 50-55 DEG C for 6-8 hours; and sequentially carrying out
ultrafiltration, adsorption decoloration and
reverse osmosis concentration treatment on the enzymolysis product. The water-soluble
fertilizer prepared by the method is rich in various amino acids, is quick in
nutrient release, and can be used for
irrigation and fertilization of crops.