15 results about "Trehalase" patented technology

The invention discloses bacillus subtilis engineering bacteria capable of efficiently producing pullulanase and application of the bacillus subtilis engineering bacteria in trehalose enzyme method production, and belongs to the technical field of genetic engineering. The invention discloses a bacillus subtilis engineering bacterium capable of efficiently producing pullulanase. The bacillus subtilis engineering bacterium comprises a pullulanase gene of which the nucleotide sequence is as shown in SEQ ID NO.7. According to the invention, the bacillus subtilis strain is used as an expression host, and the bacillus subtilis is modified through an engineering technical means, so that the pullulanase is highly expressed, and the fermentation cost of pullulanase liquid in the production process of trehalose is reduced.

Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and / or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.

Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and / or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.

Disclosed herein are genetically engineered yeast cells capable of producing xylitol and characterized by overexpression of a native glycerol kinase enzyme and an exogenous polynucleotide sequence encoding a trehalase construct comprising secretion signal operably linked to a trehalase enzyme. The genetically engineered yeast cell may additionally have a deletion or disruption of a native gene encoding an erythrose reductase and may be engineered to overexpress a native RPE enzyme, express an exogenous XPDH enzyme, express an exogenous XKS enzyme, express an exogenous XDH enzyme, overexpress a native X5PP enzyme, and / or express an exogenous X5PP enzyme.

The invention belongs to the technical field of insect genes, and particularly relates to a brown planthopper transcription factor CEBP and application thereof. According to the invention, a promoter sequence at the upstream of a brown planthopper membrane-bound trehalase gene NlTRE2 gene segment is cloned from brown planthopper, a transcription factor CEBP is predicted and verified to promote the expression of a trehalase gene promoter, and RNAi interference is utilized to prove that CEBP enables the trehalase gene NlTRE2 gene segment to have low expression; therefore, the sensitivity of the brown planthopper to buprofezin medicines is improved. The invention provides a new method for enhancing the sensitivity of brown planthopper to buprofezin drugs, and lays a foundation for understanding the relationship between energy metabolism and drug resistance of brown planthopper and for high-efficiency and low-toxicity anti-brown planthopper drugs of brown planthopper.

The application provides a diaryl ether compound and a preparation method and application thereof, and belongs to the technical field of agricultural chemicals. Substituted phenylthiol, anhydrous potassium carbonate and N,N-dimethylformamide are mixed to perform a first reaction to obtain an intermediate product; the intermediate product and methyl 2-nitro-5-chlorobenzoate are mixed to perform a second reaction to obtain the diaryl ether compound. The bioactivity determination result shows that the diaryl ether compound prepared by the application has good insecticidal activity and can be applied as a non-sugar structure insect trehalase inhibitor.

This document discloses compositions and methods for reducing trehalose during xylitol production via fermentation. The method involves contacting a substrate containing dextrose in a fermentation broth with engineered cells capable of producing xylitol, wherein the engineered cells ferment the substrate to produce xylitol. During fermentation, trehalase is present in the fermentation broth, such that the trehalose concentration at the end of fermentation is lower than that of an equivalent fermentation run without trehalase.

The invention relates to a preparation method of a double-source amino acid-containing organic water-soluble fertilizer and the prepared water-soluble fertilizer, and belongs to the technical field of agricultural bio-fertilizers. The invention aims to solve the problems of unbalanced amino acid spectrum, easy generation of peculiar smell or heavy metal residue and low nutrient release efficiency when a single raw material is adopted for preparation. The preparation method comprises the following steps: hydrolyzing an animal-derived protein raw material with an acid solution, and adding embedded slow-release vitamin C; the method comprises the following steps: carrying out gradient heating on a plant source protein raw material by using 1.0-1.5 mol / L potassium hydroxide at 90-100 DEG C, and carrying out ultrasonic-assisted hydrolysis for 3-5 hours; mixing the two hydrolysates according to the volume ratio of 3: 7, and regulating the pH to 6.5-7.0 in stages; adding a compound enzyme preparation consisting of neutral protease, papain and trehalase in a mass ratio of 3: 2: 1 into the neutralized mixed solution, and carrying out enzymolysis at 50-55 DEG C for 6-8 hours; and sequentially carrying out ultrafiltration, adsorption decoloration and reverse osmosis concentration treatment on the enzymolysis product. The water-soluble fertilizer prepared by the method is rich in various amino acids, is quick in nutrient release, and can be used for irrigation and fertilization of crops.

The invention belongs to the technical field of insect genes, and particularly relates to a transcription factor of a brown planthopper trehalase gene and application of the transcription factor. According to the invention, a soluble trehalase NlTRE1-1 gene promoter sequence is cloned from brown planthopper, an active fragment is analyzed through dual luciferase, and an efficient transcription factor Adf-1 combined with the active fragment is obtained. Furthermore, the invention designs and synthesizes an RNAi fragment combined with Adf-1, and finds that silence of Adf-1 can influence the expression of NlTRE1-1 and influence the sensitivity of brown planthopper to buprofezin drugs. The invention lays a foundation for deeper understanding of the relationship between energy metabolism and drug resistance of brown planthopper, and can be used for development of anti-brown planthopper drugs.

The invention discloses a self-induced protein expression vector of an escherichia coli T7 system and application of the self-induced protein expression vector in trehalose enzyme method production, and belongs to the technical field of molecular biology. The invention discloses a self-induced protein expression vector of an escherichia coli T7 system. The sequence of the self-induced protein expression vector is shown as SEQ ID NO. 1. Compared with a pET expression vector needing an IPTG inducer and cooling induction, the self-induced expression vector pRT7K constructed by the invention has the advantages that the inducer does not need to be added, the self-induced expression vector pRT7K is not limited to a self-induced culture medium, protein self-induced expression can be realized in a cheap LB or M9 basic culture medium, low-temperature induction is not needed, the soluble expression is high, and the vector is economic and safe. The method can further reduce the production cost of large-scale recombinant fermentation protein products such as enzyme preparations and the like, and can be used for high-throughput screening in laboratories.

There is described herein a method of treating a patient with a neurological disorder or a cardiovascular disorder, the method comprising administering to the patient trehalose in combination with a trehalase inhibitor to achieve a plasma Cmax of 1000 - 12500 ng / ml of trehalose in the patient.

The invention discloses a yak ear fibroblast immortalized cell line and a construction method thereof, and relates to the technical field of biology. The method comprises two parts of optimization: (1) optimization of a gel recovery process: beta-glucan with the final concentration of 0.2-0.5 mol / mL is added during gel recovery, so that the problems of gene degradation and low recovery rate caused by improper pH value of a gel recovery buffer solution, excessive washing, insufficient elution and the like in the prior art are effectively solved, and the recovery quality and efficiency of a target fragment are remarkably improved; (2) improvement of the cryopreservation liquid: on the basis of a traditional cryopreservation liquid (fetal calf serum: DMSO = 9: 1), a trehalase inhibitor with the final concentration of 0.1-1.0 mol / mL is added, the toxic influence of DMSO on cells is reduced, the leakage of substances in the cells is reduced, the permeability of cell membranes is stabilized, and the normal operation of signal transduction channels in the cells is guaranteed, so that the recovery survival rate of the cells is increased, and the survival rate of the cells is increased; and normal growth, proliferation and differentiation functions of cells are maintained.

Insect control nanobodies are provided. Accordingly there is provided a nanobody which specifically binds to an insect polypeptide selected from the group consisting of: a polypeptide comprising a chitin binding domain (CBD), V-ATPase subunit c, trehalase, cytochrome p450 monooxygenase, chitin deacetylase, chitin synthase and NPC1 sterol transporter, wherein binding of the nanobody to the insect polypeptide confers an insect control activity to the nanobody. Also provided are polynucleotides encoding the nanobody, host cells expressing the nanobody and methods of using it.

The application discloses a soybean immune negative regulation gene trehalase GmTRE, a coding protein thereof and application, and belongs to the technical field of crop genetic breeding. The technical problem to be solved by the application is how to improve the resistance of soybean to soybean downy mildew. To solve the above technical problem, the application provides application of a protein or a substance for regulating expression of a gene or a substance for regulating activity or content of the protein in regulation of the resistance of a plant to soybean downy mildew, and the GmTRE protein is a protein with an amino acid sequence shown in sequence 1 in the sequence listing. The application provides use of the GmTRE protein in genetic improvement breeding of soybean disease-resistant germplasm resources, and has a good application prospect.

We provide insect control nanobodies. [Solution] A nanobody is provided that specifically binds to an insect polypeptide selected from the group consisting of a chitin-binding domain (CBD), V-ATPase subunit c, trehalase, cytochrome p450 monooxygenase, chitin deacetylase, chitin synthase, and NPC1 sterol transporter, wherein the binding of the nanobody to the insect polypeptide confers insect control activity to the nanobody. A polynucleotide encoding the nanobody, a host cell expressing the nanobody, and its applications are also provided.