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72 results about "Trehalase" patented technology

The enzyme Trehalase is a glycoside hydrolase, produced by cells in the brush border of the small intestine, which catalyzes the conversion of trehalose to glucose. It is found in most animals. The non-reducing disaccharide trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is one of the most important storage carbohydrates, and is produced by almost all forms of life except mammals. The disaccharide is hydrolyzed into two molecules of glucose by the enzyme trehalase. There are two types of trehalases found in Saccharomyces cerevisiae, viz. neutral trehalase (NT) and acid trehalase (AT) classified according to their pH optima [4]. NT has an optimum pH of 7.0, while that of AT is 4.5.

Environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions

The invention discloses an environment-friendly production method for enhancing trehalose yield of beer waste yeast under stress conditions, belonging to the field of biochemical engineering and aiming to solve the problems of long trehalose extraction time, low extraction rate and difficulty in purification due to too many impurities dissolved in the prior art. The method comprises the following steps: 1) microwave treatment; 2) heat treatment; 3) helicase treatment; 4) ultrasonic crushing treatment; and 5) trehalose extraction and purification. According to the method, the yeast is under stress conditions to generate abundant trehalose; meanwhile, the activity of the trehalase is inhibited, and the yeast is subjected to helicase treatment and ultrasonic crushing treatment to rupture the yeast cells, so that almost all the trehalose is dissolved out; and thus, the method has the advantages of short treatment time, easy trehalose purification and high yield. Besides, the method does not use any strong acid, strong alkali or toxic organic solvent, and implements cleanness and environmental protection; and the waste liquid and waste residue generated in the method can be used for producing yeast extract, so almost no waste liquid or waste residue is discharged.
Owner:HEZE UNIV

Entomopathogenic fungi acidic trehalase transgenic strain, preparation method and use thereof

The invention provides an entomopathogenic fungi transgenic strain which contains a homologous or heterologous acidic trehalase constitutive exogenous gene. The preparation method of the entomopathogenic fungi transgenic strain comprises the following steps: firstly building an acidic trehalase full-length cDNA gene constitutive expression vector; establishing a submerged conidia transformation system; and finally selectively screening to obtain the entomopathogenic fungi transgenic strain. The invention also provides application of Entomopathogenic fungi acidic trehalase transgenic strain in preparing fungal pesticide. The entomopathogenic fungi transgenic strain has high efficiency of expressing trehalase; and the preparation method has the advantages of easy operation, good repeatability and good reproducibility. The entomopathogenic fungi transgenic strain can be applied to preparing the fungal pesticide. When the fungal pesticide acts on insects, through the overexpression of the acidic trehalase gene via the transgenic strain, the activity of acidic trehalase in an insect host is improved, utilization of the trehalase is accelerated and growth of entomopathogenic fungi in the insect host is promoted, thus effectively improving the insecticidal activity of the fungal pesticide such as metarhizium anisopliae, beauveria bassiana and the like.
Owner:CHONGQING UNIV

Optimized high-temperature trehalase MS-Tre capable of being expressed efficiently in aspergillus niger and encoding gene and application of optimized high-temperature trehalase MS-Tre

The invention discloses optimized high-temperature trehalase MS-Tre capable of being expressed efficiently in aspergillus niger and an encoding gene and application of the optimized high-temperature trehalase MS-Tre. The amino acid sequence of the optimized high-temperature trehalase MS-Tre is shown in SEQ ID NO.1, and the amino acid sequence of the encoding gene of the optimized high-temperaturetrehalase MS-Tre is shown in SEQ ID NO.2. Compared with a to-be-optimized encoding gene, the encoding gene of the optimized high-temperature trehalase MS-Tre can be expressed more efficiently in the aspergillus niger, and a recombinant aspergillus niger strain can produce trehalase efficiently. The trehalase has the advantages that the stability is good, disaccharide can also be hydrolyzed into monosaccharide at the high temperature, the cooling energy consumption after starch liquefaction is reduced, the utilization rate of starchy raw materials is increased, the utilization efficiency of biological energy sources is improved, and the production cost is reduced. The trehalase MS-Tre and the encoding gene thereof have certain practical significance and application value for achieving industrial production of the trehalase.
Owner:SOUTH CHINA UNIV OF TECH
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