The invention discloses an environment-friendly production method for enhancing
trehalose yield of beer waste
yeast under
stress conditions, belonging to the field of
biochemical engineering and aiming to solve the problems of long
trehalose extraction time, low extraction rate and difficulty in purification due to too many impurities dissolved in the prior art. The method comprises the following steps: 1)
microwave treatment; 2) heat treatment; 3)
helicase treatment; 4) ultrasonic crushing treatment; and 5)
trehalose extraction and purification. According to the method, the
yeast is under
stress conditions to generate abundant trehalose; meanwhile, the activity of the
trehalase is inhibited, and the
yeast is subjected to
helicase treatment and ultrasonic crushing treatment to rupture the yeast cells, so that almost all the trehalose is dissolved out; and thus, the method has the advantages of short
treatment time, easy trehalose purification and high yield. Besides, the method does not use any strong acid, strong alkali or toxic
organic solvent, and implements cleanness and
environmental protection; and the waste liquid and waste residue generated in the method can be used for producing
yeast extract, so almost no waste liquid or waste residue is discharged.