Method for improving heat-tolerance and quality-keeping of scarab green muscardine spore

A storability technology of Metarhizium anisopliae, which is applied in the field of improving the storage stability of Metarhizium anisopliae spores, and can solve problems such as product instability
CN1778891AInactive Publication Date: 2006-05-31CHONGQING UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHONGQING UNIV
Publication Date
2006-05-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention is to improve the storage stability of metarhizium anisopliae conidia. First to construct the disturb carrier of neuter trehalase RNA of metarhizium anisopliae conidia, then to found the transform system of conidiophore. Appraising by DNA imprinted, detecting the expression of its neuter trehalase gene by the RNA imprinted. Compare to the inner trehalose content, storability, heat tolerance between the transformant and the departure bacterial strain sporeú¼it can get that there is prodigious difference in the inner trehalose content, storability, heat tolerance between them. The storability, heat tolerance of the Metarhizium anisopliae conidia can be improved by this method.
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Description

technical field

[0001] The invention relates to a method for improving the storage stability of fungal spores, in particular to a method for improving the storage stability of Metarhizium anisopliae spores. Background technique

[0002] Trehalose (α-D-glucoside (1,1)-α-D-glucoside) is a non-reducing disaccharide widely present in microorganisms, plants and some lower animals. In organisms, it not only exists as a structural component and energy substance, but also plays a good non-specific protective effect on organisms and biological macromolecules under coercive conditions such as heat shock and dehydration, and is an anti-stress agent. Trehalase (EC 3.2.1.28) is a trehalose hydrolase that hydrolyzes trehalose into two molecules of glucose. Fungal trehalases are classified into acidic and neutral trehalases according to their optimum pH and regulation properties. In intact cells, trehalose is decomposed by neutral trehalase rather than acid trehalase.

[0003] Metarhizi...

Claims

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