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Method for improving heat-tolerance and quality-keeping of scarab green muscardine spore

A storability technology of Metarhizium anisopliae, which is applied in the field of improving the storage stability of Metarhizium anisopliae spores, and can solve problems such as product instability

Inactive Publication Date: 2006-05-31
CHONGQING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Metarhizium anisopliae is an important entomogenic fungus widely used in the biological control of agricultural pests such as various locusts. Like other fungal preparations, the products made from its spores are often unstable

Method used

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  • Method for improving heat-tolerance and quality-keeping of scarab green muscardine spore
  • Method for improving heat-tolerance and quality-keeping of scarab green muscardine spore
  • Method for improving heat-tolerance and quality-keeping of scarab green muscardine spore

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Embodiment

[0017] Embodiment: a kind of method that improves storability and heat resistance of metarhizium anisopliae spore

[0018] 1. Construction of the neutral trehalase ribonucleic acid (hereinafter referred to as RNA) interference carrier of Metarhizium anisopliae:

[0019] see figure 1 According to the reverse transcription DNA (cDNA) sequence (GenBank accession number: AY557612) of the neutral trehalase gene reverse transcription DNA (cDNA) sequence (GenBank accession number: AY557612) of the CQMa102 strain CQMa102 of C. Forward coding sequence (366→1508 (counting from A in the start codon ATG) and a reverse complementary coding sequence (891→366), connect these two sequences, and add one from At the promoter of the Aspergillus nidulans 3-phosphate glyceraldehyde dehydrogenase (gpdA) gene, a terminator from the Aspergillus nidulans tryptophan (trpC) gene was added at the 3' end, and then this fragment was passed through a restriction endonuclease Dicer Spe I and Not I were dou...

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Abstract

The invention is to improve the storage stability of metarhizium anisopliae conidia. First to construct the disturb carrier of neuter trehalase RNA of metarhizium anisopliae conidia, then to found the transform system of conidiophore. Appraising by DNA imprinted, detecting the expression of its neuter trehalase gene by the RNA imprinted. Compare to the inner trehalose content, storability, heat tolerance between the transformant and the departure bacterial strain sporeú¼it can get that there is prodigious difference in the inner trehalose content, storability, heat tolerance between them. The storability, heat tolerance of the Metarhizium anisopliae conidia can be improved by this method.

Description

technical field [0001] The invention relates to a method for improving the storage stability of fungal spores, in particular to a method for improving the storage stability of Metarhizium anisopliae spores. Background technique [0002] Trehalose (α-D-glucoside (1,1)-α-D-glucoside) is a non-reducing disaccharide widely present in microorganisms, plants and some lower animals. In organisms, it not only exists as a structural component and energy substance, but also plays a good non-specific protective effect on organisms and biological macromolecules under coercive conditions such as heat shock and dehydration, and is an anti-stress agent. Trehalase (EC 3.2.1.28) is a trehalose hydrolase that hydrolyzes trehalose into two molecules of glucose. Fungal trehalases are classified into acidic and neutral trehalases according to their optimum pH and regulation properties. In intact cells, trehalose is decomposed by neutral trehalase rather than acid trehalase. [0003] Metarhizi...

Claims

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Application Information

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IPC IPC(8): C12N1/15C12N15/80
Inventor 夏玉先胡宗利王中康殷幼平彭国雄
Owner CHONGQING UNIV
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