69 results about "Metarhizium" patented technology

The present invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same. The Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.

Microsclerotia of entomopathogenic fungi, including Metarhizium species, Beauveria species, and Lecanicillium species, may be produced. These microsclerotia are effective for the control of a wide variety of insect pests, particularly soil-dwelling insect pests.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention belongs to the field of agricultural production and discloses a formula of a biological source bait for preventing and controlling Solenopsis invicta Buren and a use method thereof. The formula is used for preventing and controlling invasive insect pest Solenopsis invicta Buren. The biological source bait utilizes one or more active ingredients such as Metarhizium fkavoviride, verticillum lecanii, bacillus thuringiensis and beauveria bassiana. The active ingredients and baits such as pork, konjac glucomannan, sodium chloride, egg yolk powder, monosodium glutamate and sucrose are mixed to form an insecticide, the insecticide is put on the upper edges of a Solenopsis invicta Buren nest or is placed at a position where there is Solenopsis invicta Buren invasion hidden danger so that prevention and control of Solenopsis invicta Buren are realized. The formula is simple and is easy to use. Through virus transmission from worker Solenopsis invicta Buren to ant Solenopsis invicta Buren, nest elimination is realized. The active ingredients are insect pathogens and can effectively prevent environmental damage caused by pesticide application.

The invention relates to the field of biological control, in particular to a high-efficiency insecticidal fungus and applications thereof. The invention provides a high-efficiency insecticidal fungus metarhizium strain MBJQH 2-2 which effectively prevents and control scaraboid underground pests, is friendly and compatible with the environment and has a preservation number of CGMCC (China General Microorganism Conservation Center) No. 4275. The metarhizium strain of the invention is rooted in the nature, returns to the nature, is friendly and compatible with the environment, pollution-free and residue-free, has unique high-efficiency control action on the scaraboid underground pests, can be used as a pollution-free novel pesticide and used in an innoxious production process of underground pests, such as crops, fruit trees, forest, lawn, and the like. Proved by field prevention and control tests, the invention has favorable prevention effect and reaches the prevention effect on a plurality of important scaraboid larvae up to 60-70 percent.

The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergisticeffect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.

The invention discloses a Metarhizium and Empedobacter brevis compounded insecticide and an application thereof. The insecticide is prepared from Metarhizium, Empedobacter brevis and insecticide acceptable auxiliaries. The insecticide has obvious synergistic effect, and insecticidal toxicity of single chemical can be improved obviously; meanwhile, the action effect after compounding is equivalentto the effect of a chemical agent. The insecticide is compounded from two single biological insecticidal agents as main agents, has good insecticidal activity for defoliator of forestry, has good environmental compatibility and has great significance for improving ecological environment and increasing human health level when widely applied. A production process is simple, use quantity of a pesticide can be reduced substantially, drug resistance of pests can be delayed, and the insecticide has obvious effect on pollution-free control and continuous integrated control of the pests.

The invention discloses application of metarhizium anisopliae CQMa421 strains to preparation of a pesticide for killing lissorhoptrus oryzophilus kuschel. Wettable powder produced with the strains consists of kieselguhr (30-40%), nanometer silicon carbide (0.5-1%), sodium carboxymethylcellulose (3-5%), tween-80 (3-5%), OP-10 (3-5%) and metarhizium anisopliae CQMa421 dry spore powder (44-60%). The pesticide for killing the lissorhoptrus oryzophilus kuschel has very good wettability, storage characteristics and uvioresistant characteristics, and the lissorhoptrus oryzophilus kuschel can be effectively controlled.

The invention discloses an eelworm killing metarhizium and the application thereof. The metarhizium is metarhizium anisopliae var.anisopliae HYN-1-2 which is collected in the China general microbiological culture collection center on 31st October, 2011 and the collection number is CGMCC No.5433. The metarhizium and metarhizium preparations are used for controlling plant meloidogyne; the controlling effect can reach 50-70 percent and is relatively stable; the active constituents of the metarhizium and the metarhizium preparations are good in fixed planting effect in soil, non-toxic and pollution-free in use, high in performance price ratio, convenient in application, and wide in application prospect.

The invention discloses a fertilizer for controlling red roots and red fruits of dwarf lilyturf tuber. The fertilizer is prepared from the following components including a basic fertilizer and an active bacterium mixture, wherein the basic fertilizer is prepared from the following components in parts by weight: 11.1-13.7 parts of urea, 26.7-30.5 parts of ammonium sulfate, 24.4-27.5 parts of diammonium phosphate, 22-24 parts of potassium sulfate, 1.5-4.8 parts of zinc sulfate, 0.8-3.3 parts of boric acid, 0.5-2.1 parts of chelated iron, 1-4 parts of manganese sulfate, 10-14.5 parts of amino acid, 1-4 parts of avermectin and 1-3.5 parts of compound sodium nitrophenolate; the active bacterium mixture is prepared from the following components in parts by weight: 0.8-1.3 parts of metarhizium, 1.3-2.7 parts of trichoderma harzianum, 60-90 parts of plant straw, 15-26 parts of a cake fertilizer, 1.0-1.5 parts of ammonium bicarbonate and 1.8-2.3 parts of a straw decomposition maturing agent. According to the fertilizer disclosed by the invention, with the matching use of the basic fertilizer and the active bacterium mixture, the incidence of the red roots and the red fruits of the dwarf lilyturf tuber can be effectively reduced, and the dwarf lilyturf tuber is promoted to healthily grow.

A method of treating drain systems containing organic matter and providing a pesticide for killing insects is provided. Introducing a bacterial culture into a drain system that is operable to metabolize organic matter will reduce the quantity of the organic matter in the drain system. Introducing a biocidal amount of fungus culture such as, Metarhizium, into the drain system allows for exposure of the fungus to insects which feed on the organic matter in the drain system. The insects, once infected, will spread the fungus to other insects. The fungus, provided in suitable quantities, will kill the insects. The insect is infected through contact or ingestion. The drain system can be a residential or commercial drain system. The fungus can be intended to kill insects that are cockroaches or other soft-bodied insects. The bacterial cultures can be separately sprayed into the drain or dispersed together into the drain with the fungus. The bacterial cultures and the fungi are maintained separately and then mixed for the treating of the drain. The cultures are maintained together prior to introducing them into the drain. The cultures can be dispersed as a spray, a powder, a liquid, or a foam.

The invention relates to an insecticide compound with the complementary action and the synergetic effect. The insecticide compound comprises the active ingredients of guadipyr and metarrhizium anisopliae, wherein the weight ratio of guadipyr to metarrhizium anisopliae is (1 to 60):(70 to 1). Auxiliaries and fillers are added into the effective active ingredients to form wettable powder, water dispersible granules and a suspending agent. The insecticide compound disclosed by the invention can be used for delaying the generation of the pest resistance to insecticides and can be widely applied to the field of agriculture.

The invention discloses a microbial pesticide for preventing and treating fungal diseases. The microbial pesticide comprises the following materials in parts by weight: 22-40 parts of verticillium lecanii, 5-17 parts of corn flour, 11-20 parts of polyisobutylene succinimide, 25-36 parts of malic acid, 15-32 parts of diatomite, 3-7 parts of metarhizium powder, 15-30 parts of trichoderma harzianum, 5-19 parts of amino-oligosaccharin, 6-9 parts of beta-cypermethrin, 45-62 parts of streptomyces diastatochromogenes D and 15-18 parts of arteannuin. The microbial pesticide is applied to preventing and controlling fungal diseases of plants and is similar with or slightly superior to general chemical agents in effect, and a new way is provided for the development of agricultural fungicides.

The invention belongs to the technical field of biocide containing microbial fungi, and more specifically relates to a Metarhizium and bacterium composite pesticide used for killing Spodoptera frugiperda. The Metarhizium and bacterium composite pesticide comprises Metarhizium and bacteria, and possesses excellent prevention and control effect on Spodoptera frugiperda.

The invention discloses a Metarhizium taii GYYA0601 strain capable of producing broad-spectrum antibiotics and application thereof. The preservation number of the Metarhizium taii GYYA0601 is CGMCC No.2880. The Metarhizium taii GYYA0601 strain is used for producing the antibiotics by fermentation; a fermentation method comprises the steps of strain activation, primary seed preparation and fermentation; and the obtained fermentation product has broad-spectrum antibiotic activity.

The invention discloses a plant and microorganism compound pesticide. The pesticide is characterized in that the pesticide comprises the following raw materials in parts by weight: 20-30 parts of aconite main roots, 20-30 parts of Cynanchum komarovii, 10-15 parts of Pogostemon cablin, 10-15 parts of Artemisia annua, 8-12 parts of tuber fleece-flower roots, 4-8 parts of powder of Bacillus thuringiensis, 2-4 parts of Metarhizium powder, 70-80 parts of a carrier, 5-12 parts of a wetting agent, and 2-5 parts of a dispersant. The materials of the pesticide comprise plant raw materials and microorganism raw materials which are compounded for usage, and the pesticide has the advantages of complementary advantages, wide insecticidal spectrum, substantial insecticidal effects without residuals and pesticide resistance, and green and environmental protection.

The invention discloses a green muscardine fungus preparation for killing spodoptera frugiperda. The method comprises the following steps of: S1, separating from collected larvae of ailing spodopterafrugiperda to obtain a green muscardine fungus strain, culturing the green muscardine fungus strain on a Sabouraud Dextrose Agar medium, transferring conidia to a new culture medium for purification,and then carrying out single spore separation to obtain purified green muscardine fungus MRCNTS20190814; S2, mixing green muscardine fungi with vegetable oil, a surfactant and an antioxidant; and S3,collecting and detecting after the mixing is completed. The green muscardine fungus preparation has a scientific and reasonable structure, and is safe and convenient to use; the insecticidal efficiency of the green muscardine fungi can be obviously improved by the new proportion; the death rate of the oil agent can reach 50% or above after about 4 days, the death rate of the water agent still doesnot reach 50% after 8 days, and the problem that the insecticidal effect is reduced due to the fact that spores germinate too early due to the fact that a traditional spodoptera frugiperda fungal insecticide is generally an oil suspending agent and water is added for dilution in the release process is solved.

The invention provides a compound biological pesticide and a preparation method and application thereof. The compound biological pesticide takes metarhizium spores and boric acid as active ingredients, wherein the content of boric acid is less than or equal to 2%. By the arrangement, not only the additive effect but also synergistic effect in pest control can be achieved, the effective time is greatly shortened after compounding, pest killing effect is realized in less time, and boric acid is used much less than common chemical pesticides on the market.

The combination of keratin hydrolysate with viable cells or infectious propagules of a fungal biological control agent such as Paecilomyces species, Metarhizium species, Beauveria species, and / or Verticillium species, provides effective control of soil-dwelling or subterranean insects, including termites, and particularly those belonging to the family Rhinotermitidae, such as the Formosan subterranean termite and native North American subterranean termites. In use, a foam-forming composition comprising the keratin hydrolysate and an insecticidally effective amount of the fungus are applied to the insects, the locus of the insects, to material susceptible to infestation by said insects, or to the locus of material susceptible to infestation by said insects.

The invention belongs to the technical field of biological control of plant diseases, and particularly relates to an application of a metabolite of metarhizium anisopliae in preparation of gray mold biocontrol agents. The used metarhizium anisopliae IBCCM321.93 has significant inhibition on mycelial growth, sclerotium formation, sclerotium germination, spore germination and the like of botrytis cinerea. Fermentation filtrate of the strain has prevention and treatment effects on gray mold on plants and gray mold on picked fruits. In addition, volatile gas generated by the metarhizium anisopliaealso has a good prevention and treatment effect on gray mold on fruits in the storage period.

The present invention discloses a Metarhizium anisopliae strain and its application. The present invention relates to a Metarhizium anisopliae strain, and the strain is Metarhizium anisopliae; a preservation name is Metarhizium umaniensis (JXAS-03), the strain is preserved at the China Type Culture Collection Center, and a preservation address is China Type Culture Collection Center of Wuhan University, Bayi Road, Hongshan District, Wuhan, Hubei Province; a preservation number is August, 14th, 2019; and a preservation number is CCTCC M 2019626. The strain of the present invention has a stronginsecticidal ability against Chinese mole cricket pests, and provides a new material for the control of Chinese mole cricket pests.

The invention discloses a microbial insecticide containing Metarhizium having traditional Chinese medicine activity. According to the present invention, 0.2-0.5% by volume of chitosan is added to a culture medium having traditional Chinese medicine activity, Metarhizium is inoculated into the obtained culture medium according to a volume ratio of 4-8%, and solid fermentation is performed until theconcentration of microorganism reaches 4.0 billion strain / g so as to prepare the microbial insecticide containing Metarhizium having traditional Chinese medicine activity, wherein the culture mediumhaving traditional Chinese medicine activity is prepared by uniformly mixing milkvetch root, salvia miltiorrhiza, fructus ligustri lucidi, acanthopanax senticosus, licorice root, artemisia carvifolia,eucommia ulmoides, viscum coloratum, stemona japonica, sophora flavescens ait and azadirachta indica; and the obtained microbial insecticide can solve the problems of less number of microorganisms, low activity and the like of the microbial insecticide in the prior art, and can increase the number of microorganisms and improve the activity of microorganisms so as to provide the improved insecticidal effect.

The invention relates to a method for killing white ants in a tree body and repairing ant paths. According to the method, bentonite, verticillium, metarhizium and agritol are used and prepared into drug paste, a grease gun is used for injecting the prepared drug paste into defecation holes of the white ants until the drug paste overflows from the injection part, and the bentonite solidifies and fully fills the whole ant paths, so that the white ants die through asphyxia and mechanical injury, meanwhile, the effect of repairing the ant paths is achieved, and the effects of killing the white ants and repairing the ant paths are achieved.

The invention discloses a nutritional medium used for grape cultivation, and a preparation method thereof. The nutritional medium used for grape cultivation comprises, by mass, 20 to 40 parts of bonemeal, 10 to 15 parts of decomposed chicken manure, 10 to 25 parts of fly ash, 10 to 20 parts of coconut coir, 20 to 30 parts of sea sand, 5 to 10 parts of ammonium sulfate-nitrate, 10 to 12 parts of diammonium phosphate, 5 to 10 parts of zinc gluconate, 2 to 10 parts of polyaspartic acid, 1 to 3 parts of chitosan, 5 to 8 parts of vitamin B6 powder, 2 to 6 parts of vitamin A powder, and 2 to 5 parts of metarhizium. The nutritional medium is capable of improving water retention capacity, fertilizer slow release effect, and grape disease resistance and insect resistance, and ensuring grape quality.

The present invention relates to a method for harmless treatment of municipal sludge, which belongs to the technical field of environmental protection and pollutant treatment. The prior municipal sludge treatment method mainly includes landfill and incineration, and has the problems of high investment and secondary pollution. The harmless treatment method comprises dewatering sludge, mixing with hull, sterilizing at a high temperature, inoculating Metarhizium, and performing aerobic fermentation to obtain biological pesticide containing Metarhizium. The invention is suitable for producing cheap biological pesticide with municipal sludge as raw material, achieves harmless treatment of municipal sludge, and simultaneously utilizes municipal sludge resources effectively. The inventive product can be used as both biological pesticide and fertilizer, and is widely applicable for upland field crops, nursery stocks, flowers and lawns.

The invention discloses Metarhizium spore powder produced by an inclined-form fermentation and reaction bin, which adopts straws as major materials and wheat bran and corn meal as accessories while KH2PO4, MgSO4 and CuSO4 are used as fermentation-used culture materials and metarhpium anisophiae is adopted as a strain and then intermittent culture is carried out to the fermentation bin to produce spores. A method includes the steps: the straws are put into a material gas explosion system for carrying out gas explosion; the straws after gas explosion react with the wheat bran and the corn meal in a chelated reaction bin; after the reaction, the materials are put into the fermentation bin and the culture materials and the metarhpium anisophiae are added, and then ventilation and oxygen supply are carried out in the fermentation bin and fermentation lasts for 96 hours; and a packet receiver is used for collecting spores. The spore powder has the advantages that products can effectively control over 130 kinds of plant diseases and insect pests and wide material selection is realized, and more straw wastes are utilized with environmental production, no toxicity, no innocuity, low production cost, high yield, no easy cross contamination and wide application prospects.

The invention discloses a pharmaceutical composition for preventing and controllting diseases and insect pests in a seedling stage of an organic tea garden, and also discloses a preparation method ofthe pharmaceutical composition for preventing and controlling diseases and insect pests in the seedling stage of the organic tea garden. The preparation method comprises the following steps: stirringberberine, anonaine, polyoxyethylene octylphenol ether-10 and ethanol to obtain a premixed solution; adding sodium bentonite into water, stirring to form slurry, adding octadecyl trimethyl ammonium chloride and sodium pyrophosphate, stirring, filtering, wdusting, drying and crushing to obtain pretreated bentonite; grinding the pretreated bentonite, eucalyptus grandis essential oil, tea tree oil and peppermint oil for 4-10 minutes at the grinding speed of 10000-12000 r / min and the grinding pressure of 1.2-1.4 MPa, controlling the grinding temperature to be less than or equal to 40 DEG C, addinga metarhizium anisopliae agent, and uniformly mixing to obtain pretreated essential oil; and fully stirring the pretreated essential oil and the premixed solution, then adding a coalescing agent, uniformly stirring, drying and crushing to obtain the pharmaceutical composition for preventing and controlling diseases and insect pests in the seedling stage of the organic tea garden.

An antibacterial fly edible fungus biological culture medium is characterized in that: (1) raw material selection: local materials can be obtained according to local conditions; (2) raw material preparation: according to the type of edible fungi, fertilizers are added to prepare a base suitable for the growth of target edible fungi. (3) Fermentation of raw materials: add appropriate amount of water to the raw materials, and then stack retting treatment, during which the piles are turned over in good time until the fermentation is completed; (4) Antibacterial fly fungus spore culture and counting: inoculate Metarhizium anisopliae of scarab on potato dextrose agar On the culture medium, cultivate at 25°C for 8-13 days to obtain the spores of Metarhizium anisopliae, and use sterile water to make a spore suspension with a concentration of 1020-1040 / ml; (5) Add antibacterial fly strains: according to Calculate the volume of fermented raw materials, add the spore suspension of Metarhizium anisopliae to the culture medium, and finally make the spore concentration in the edible fungus culture medium reach 103-108 / ml, that is, the antibacterial fly edible fungus biological culture medium is obtained.