118 results about "Peptidoglycan" patented technology

The technology described herein relates to methods of inhibiting Peptidoglycan Recognition Protein 1 (Pglyrp1) in order to improve immune-checkpoint inhibitor treatments for cancer and autoimmune diseases.

The invention provides engineering lyase Art-15 with broad-spectrum antibacterial activity, antibacterial peptide NZ2114 and bacteriophage lyase PlySs2 are subjected to functional fusion through an optimally designed flexible linker, and an efficient bactericidal effect on Staphylococcus aureus (Staphylococcus aureus) and Streptococcus spp.) is achieved. The engineering lyase Art-15 has the advantages that the engineering lyase Art-15 has broad-spectrum antibacterial activity, the antibacterial peptide NZ2114 and the bacteriophage lyase PlySs2 are subjected to functional fusion through the optimally designed flexible linker, and the engineering lyase Art-15 has broad-spectrum antibacterial activity; the lyase Art-15 overcomes the defects of narrow host spectrum and insufficient splitting activity of the lyase through a synergistic destruction mechanism of targeting bacterial cell wall peptidoglycan. Experiments show that the minimal inhibitory concentration (MIC) of the lyase Art-15 on staphylococcus aureus in vitro is lower than 2 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus agalactiae is lower than 1 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus dysgalactiae is lower than 2 mu g / mL, and the sterilization rate of the lyase Art-15 can reach 97% or above within 30 minutes by 2 times of MIC. The lyase Art-15 disclosed by the invention has important application value in the fields of drug-resistant bacterium infection treatment, medical instrument disinfection and livestock breeding.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention discloses a bacteriostatic composition and an application thereof in a hand sanitizer, the bacteriostatic composition comprises the following components by mass: 3-5% of lysozyme, 4-8% of an enzyme protection system, 8-12% of a plant bacteriostatic extract, 10-15% of a biological matrix carrier, 5-8% of a stable protection system, and the balance of water. According to the antibacterial composition, a three-dimensional network structure is formed through the carboxymethyl chitosan-sodium alginate compound, a protective layer with good biocompatibility can be formed on the skin surface, and the skin retention time of lysozyme and plant antibacterial active ingredients is remarkably prolonged. According to the invention, lysozyme and plant antibacterial components are adopted to form multiple antibacterial mechanisms: the lysozyme specifically hydrolyzes peptidoglycan in bacterial cell walls, and active components in the plant antibacterial extract destroy bacterial cell membranes and inhibit bacterial metabolism. The double-attack mechanism has a stronger antibacterial effect than a single component, and can prevent bacteria from generating drug resistance at the same time.

The invention relates to a compound of formula Ia and / or formula Ib: or a pharmaceutically acceptable salt, solvate or prodrug thereof, where the groups are defined herein. The invention also relates to a pharmaceutical formulation comprising the compound for treating or detecting a microbial infection in a subject, a method of determining antimicrobial resistance of a microbial infection using the compound, and a method of determining an effective dose of one or more antimicrobial agents to kill a microorganism using the compound.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of inflammatory factor TNF-alpha and IL-1 beta mRNA (messenger Ribonucleic Acid) genes. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1 beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention provides a preparation method of a novel PEDV (porcine epidemic diarrhea virus) subunit vaccine, and belongs to the technical field of biology. The novel PEDV subunit vaccine is prepared by adopting a method comprising the following steps: (1) treating lactococcus lactis by using a high-pressure damp-heat method to obtain peptidoglycan particles; (2) treating the peptidoglycan particles by adopting an ultrahigh-pressure microjet technology to obtain a peptidoglycan carrier with the particle size of 280-850nm; and (3) mixing the peptidoglycan carrier with a PEDV subunit antigen of which the amino acid sequence is SEQ ID NO: 1 to obtain the novel PEDV subunit vaccine. The novel PEDV subunit vaccine comprises a peptidoglycan carrier with a specific size, can improve the in-vivo presentation efficiency of an antigen and rapidly induce efficient body fluid and mucous membrane immune response, and is of great significance to research and development of vaccines for porcine epidemic diarrhea diseases and the like.

Provided herein is a rapid amplifiable readout of bacterial growth, such as an ELISA paired with metabolic labeling. Methods comprise a) contacting a sample from a subject suffering from a bacterial infection with an antibiotic; and b) determining the effect, if any, of the antibiotic on incorporation of a single- or di-D-amino acid into peptidoglycan of the bacterial cell or incorporation of trehalose into the bacterial cell envelope, wherein an antibiotic that reduces, as compared to control without the antibiotic being present, incorporation of the D-amino acid into peptidoglycan of the bacterial cell or inhibited incorporation of trehalose into the bacterial cell envelope is an antibiotic that is effective.

The invention relates to the technical field of genetic engineering, and discloses an engineering bacterium for high-yield production of D-pantothenic acid based on sugar uptake regulation and a preparation method of the engineering bacterium. The method comprises the following steps: firstly, stabilizing an RNAP promoter open complex formed on an rRNA (Ribonucleic Acid) gene by intensively expressing a necessary global regulation transcription factor CarD of mycobacterium neoaurum; secondly, performing enhanced expression on mycobacterium neoaurum Rpf / Sps family protein RpfB which serves as a resuscitation promoting factor to play a role and catalyze peptidoglycan synthesis to accelerate cell wall growth; meanwhile, the inactivated cell wall lipoprotein Lpp accumulates the outer membrane vesicles; in addition, lipoprotein NlpI responsible for adjusting the activity of endopeptidase MopS and PBP4 is deleted, and the super vesicles are generated; in addition, the mutant membrane lipid asymmetric pathway protein MlaE disturbs the homeostasis of the outer membrane and increases the formation of vesicles; in addition, the membrane anchoring protein TolA is destroyed to improve the membrane permeability; and finally, the escherichia coli gene engineering strain with greatly improved D-pantothenic acid production performance is obtained.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

The invention relates to the field of chemical synthesis and biomedical materials, and discloses a multifunctional nano-drug and a preparation method and antibacterial application thereof, and the multifunctional nano-drug is mainly composed of a copper sulfide nano-carrier, a bacterial targeting photosensitizer and glucose oxidase (GOx). Wherein the copper sulfide nano-carrier (CuS NPs) has a hollow mesoporous structure and can effectively load drug molecules. The bacterium targeting photosensitizer, namely, D-cysteine functional modified pentafluorophenyl mycogreen (FBC-Cys), can be used for targeting bacteria by utilizing the characteristic that free D-type amino acid is integrated to the end of peptidoglycan on the surface of a cell wall in the cell wall synthesis process of the bacteria. Glucose oxidase (GOx) can efficiently consume glucose in the bacterial microenvironment, and the hunger treatment effect is achieved. The hollow mesoporous copper sulfide nanoparticles are prepared by adopting a one-pot hydrothermal method, synthesis raw materials are easy to obtain, and the method is simple and rapid. And the FBC-Cys and the GOx are loaded to the cavities and the surfaces of the copper sulfide nanoparticles through a physical adsorption effect. The multifunctional nano-drug can target bacterial cell walls, change the bacterial microenvironment, integrate photodynamic therapy and hunger therapy, and achieve a multifunctional antibacterial effect. The antibacterial hydrogel provided by the invention has excellent antibacterial effect and biocompatibility, and can play an excellent role in treatment of future bacterial infection.

The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.

The application discloses the application of a fruit fly Kelch protein in the technical field of biotechnology in the preparation of a fruit fly Toll path anti-bacterial immune defense product, the fruit fly Kelch protein positively regulates the fruit fly Toll path anti-bacterial immune defense, and the expression loss of the fruit fly Kelch protein leads to the decline of the immune defense capability of the fruit fly to gram-positive bacteria infection and the decrease of the induced expression of a Toll path targeted antibacterial peptide gene. The application discloses that a Kelch-PGRP-SC1a regulation axis is an important regulation module in the Toll path anti-bacterial immune defense of Drosophila melanogaster, provides a brand-new perspective for the expression regulation research of peptidoglycan recognition protein, discovers a new function of Kelch as a founder member of the BTB-Kelch protein family, enriches the function spectrum of the BTB-Kelch protein family, supplements the upstream regulation link of the fruit fly Toll natural immune path, and perfects the immune response regulation network of gram-positive bacteria infection.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

The present invention provides a probiotic combination for inhibiting Helicobacter pylori, Lactobacillus rhamnosus INM3105, which was deposited in Guangdong Microbial Culture Collection Center on April 11, 2024, with a deposit number of GDMCC NO: 64513. Lactobacillus plantarum INM3106 was deposited in Guangdong Microbial Culture Collection Center on April 11, 2024, with a deposit number of GDMCC NO: 64514. The advantages are: the peptidoglycan extracted from Lactobacillus rhamnosus and Lactobacillus plantarum can effectively inhibit Helicobacter pylori. The ability of the peptidoglycan ratio of the two strains to inhibit Helicobacter pylori is further improved than that of a single strain of the same concentration.

The invention discloses a tannic acid functionalized micromotor with functions of enhancing bacterium capture and bacteriostasis, and a preparation method and application thereof. The micromotor takes Cu7S4 / vSiO2 particles with a virus-like structure as a carrier, and tannic acid is loaded on the surface of the carrier through a self-assembly method. On one hand, TA has an antibacterial effect, and rich peptidoglycan on cell walls of gram-positive bacteria has a high affinity effect; on the other hand, a virus-like structure with a spike structure has relatively high adhesion to bacteria, and the micromotor Cu7S4 / vSiO2 / TA has an enhanced capture effect on gram-positive bacteria; in addition, the photothermal effect and self-driving generated by Cu7S4 under the irradiation of infrared light can cooperate with TA to realize efficient sterilization.

The present invention relates to a bacterial strain containing an open reading frame encoding a recombinant protein, thereby controlling a functional promoter. The present invention is characterized in that the bacterial strain contains an open reading frame encoding a mutant peptidoglycan-associated lipoprotein (PAL protein), thereby controlling a functional promoter, wherein the PAL protein is mutated so that the PAL protein does not contain a membrane anchor of the bacterial outer cell membrane. The present invention also relates to plasmids encoding recombinant proteins and mutant PAL proteins, and to a method for fermenting and producing recombinant proteins using the bacterial strain according to the present invention. In this way, compared with the prior art, an increased product yield in the culture residue can be achieved without causing a large number of bacterial cells to die.

The invention relates to the technical field of biochemistry, and discloses a hydrolase for splitting bacterial peptidoglycan causing chronic inflammatory diseases, the hydrolase is separated from a human intestinal microbe genome, and the hydrolase can hydrolyze a peptidoglycan layer of gram-positive pathogenic bacteria in a targeted manner, so that the bacterial peptidoglycan has the advantages that the bacterial peptidoglycan has a good anti-inflammatory effect, and the bacterial peptidoglycan has a good anti-inflammatory effect. The compound has high efficiency, specificity and low drug resistance and has a synergistic effect with existing antibiotics; the preparation method of the hydrolase comprises the following steps: according to an amino acid sequence, selecting escherichia coli preferred codons, and artificially synthesizing a gene sequence for coding the hydrolase; cloning the synthetic gene into an expression vector to construct a recombinant expression plasmid; transforming the recombinant expression plasmid into escherichia coli, and carrying out induced expression; the hydrolase is obtained through a separation and purification step. According to the hydrolase for splitting the bacterial peptidoglycan causing the chronic inflammatory disease, a peptidoglycan layer of gram-positive bacteria can be efficiently hydrolyzed through the hydrolase, so that the bacteria are rapidly split, and the antibacterial effect is improved.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The invention belongs to the field of aquatic bacteria detection, and relates to a fluorescent array sensor as well as a preparation method and application thereof. The fluorescent array sensor comprises a plurality of DNA aptamers, the DNA aptamers respectively target lipopolysaccharide, peptidoglycan and flagellin, the DNA aptamers of the target lipopolysaccharide are EA5 and LA27, the DNA aptamer of the target peptidoglycan is Antibac2, and the DNA aptamer of the target flagellin is Aptamer 3; the sequence of the EA5 is shown as SEQ ID NO.1, the sequence of the LA27 is shown as SEQ ID NO.2, the sequence of the Antibac2 is shown as SEQ ID NO.3, and the sequence of the Aptamer 3 is shown as SEQ ID NO.4. The invention further discloses a kit for detecting the content of the EA5, the LA27, the Antibac2 and the Aptamer. According to the present invention, the six bacteria can be simultaneously detected, the detection limit is low, the operation process is simple, and the detection accuracy can achieve 97.6%.

The invention discloses a mattress material based on modified fibers and a preparation method of the mattress material. The mattress material comprises nylon 66 fibers, low-melting-point fibers and the modified fibers. The low-melting-point fiber is ES bi-component parallel composite fiber composed of polypropylene and polyethylene, and the modified fiber is prepared by mixing a modified polymer and a modified filler to prepare a spinning solution and then carrying out electrostatic spinning and ultraviolet irradiation. Molecular chains of the modified polymer contain rich furan rings and thiophene rings, synthesis of bacterial cell walls can be destroyed, the furan is grafted with oximido, and a formed furan oxime ester unit can generate highly unstable and reactive free radicals, so that metal ions in cells are unbalanced to cause change of cell metabolism homeostasis. The lysozyme fixed on the modified filler can be hydrolyzed to form peptidoglycan of bacterial cell walls, so that the cell walls are broken, and the material has good antibacterial performance.

The invention relates to the technical field of medical instruments, and discloses a comprehensive acupuncture and massage treatment device which comprises a hollow block with holes, a heating box fixedly mounted at the bottom of the hollow block with holes, a plurality of heating pipes fixedly mounted in the heating box, two reset springs fixedly mounted on the inner wall of the top of the heating box, and a disinfection mechanism, the disinfection mechanism comprises a movable rectangular plate fixedly mounted at the top ends of two reset springs, a disinfection box is fixedly mounted on the right side of the heating box, a through groove is formed in the left side of the disinfection box, the left end of the through groove extends into the heating box, and a sliding drawer is slidably mounted in the disinfection box. Peptidoglycan and other components in spore walls of the bacteria can be hydrolyzed under the action of high-temperature steam, moisture can also enter the spores, internal core areas are expanded, finally, the spores die, the powerful sterilization effect is achieved, and cross infection caused by the fact that bacteria which are not sterilized completely remain on the needle is avoided.

The invention relates to the technical field of biological adsorption materials, in particular to modified peptidoglycan with an efficient adsorption effect and a preparation method and application of the modified peptidoglycan. The preparation method comprises the steps of grafting and modifying carboxyl / hydroxyl of peptidoglycan, so that multi-site and multi-mode functional modification on the surface of the peptidoglycan is realized. The physicochemical properties of functional groups on the surface of peptidoglycan are changed through modification treatment, the density and electronegativity of carboxyl and hydroxyl functional groups on the surface of peptidoglycan are improved, the hydrogen-bond interaction and electrostatic adsorption capacity between the peptidoglycan and biotoxin are enhanced, and the adsorption efficiency is improved. In addition, the peptidoglycan is subjected to carboxyl and hydroxyl modification at the same time, and the tetrodotoxin adsorption effect of the peptidoglycan can be further improved. The technology provides a new strategy for functionalization of natural polymer materials, has good theoretical popularization value and application prospects, and is suitable for the fields of food safety, removal and purification of biotoxins and the like.

The invention relates to the first hydrolase that specifically digests the archaeal type of a peptidoglycan (PG), the major component of the cell wall of methanogens, named Talosaminidase. The enzyme consists of a signal peptide, several bacterial IG domains, a glycosyl hydrolase, several archaeal PG binding domains and a peptidase domain. The double enzymatic activity for the glycosyl hydrolase and the peptidase maximizes the lysis of archaeal cells with a cell wall of the archaeal PG-type. Engineering the enzyme by removing the signal peptide and bacterial IG domains and keeping only the archaeal PG binding, glycosyl hydrolase and peptidase made the enzyme easier to express and purify, without negatively influencing the enzymatic activity. Therefore, engineered Talosaminidase can be used to regulate populations of archaeal methanogens in the industrial, agricultural and medical settings, and consequently methane production.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of mRNA genes of inflammatory factors TNF-alpha and IL-1beta. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention provides a composition, a medicine and a preparation method and application thereof, the composition relates to the technical field of medicine, the composition comprises a carrier and an active component loaded on the carrier, the carrier contains cell wall peptidoglycan, the active component comprises a first active component with a specific treatment effect on a focus, and the first active component comprises a second active component with a specific treatment effect on the focus. And a second active ingredient having affinity to the lesion area, wherein the first active ingredient comprises bile acid; the second active ingredient includes a targeting peptide. The composition disclosed by the invention can activate endothelial cells in a targeted manner, and can reduce various heart and brain diseases, metabolic diseases, infectious diseases, allergic diseases and the like caused by endothelial cell dysfunction, so that diseases and death caused by the dysfunction are reduced.

The invention is directed to immunological compositions of one or more peptides containing epitopes of PGN, LTA and LPS molecules that induce an immunological response in a mammal, and to multiple antibodies that bind to these epitopes. Immunological compositions and antibodies disclosed herein can be used in the treatment and / or prevention of human health disorders such as bacterial sepsis, inflammation, cancers, tumors, inflammatory diseases and disorders, and neurodegenerative disorders such as, but not limited to Alzheimer's disease, frontotemporal dementia, chronic traumatic encephalopathy (CTE), Lewy body dementia and / or limbic predominant age-related TDP-43 encephalopathy (LATE).

The invention belongs to the field of food safety, and discloses application of cell wall lyase LysM15 in preparation of a bacillus cereus inhibitor. The cell wall lyase LysM15 has the functions of specifically recognizing the cell wall of the bacillus cereus and efficiently cracking the peptidoglycan layer of the bacillus cereus. The LysM15 not only can obviously inhibit the germination of bacillus cereus spores and remove biofilms of the bacillus cereus spores, but also can keep higher enzyme activity in a temperature range of 4-50 DEG C and in a high-salt environment. The lyase has good biocompatibility, growth and pollution of bacillus cereus in fresh beef can be effectively reduced, the potential of the lyase serving as a food preservative is shown, and a safe and efficient solution can be provided for food preservation.