54 results about "Peptidoglycan" patented technology

The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.

The invention discloses a biological preparation suitable for young pigeon growth and a preparation method and application thereof, and belongs to the technical field of feeds, the biological preparation comprises the following components by weight: 8-40 parts of a microbial agent, 1-10 parts of an additive, 1-10 parts of an antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite; the microbial agent comprises bifidobacterium animalis, lactobacillus acidophilus, lactobacillus paracasei and lactobacillus reuteri; the additive comprises at least one of yeast beta-glucan and astragalus polysaccharide. According to the biological preparation suitable for young pigeon growth, montmorillonite is used as a base material, under the combined action of the microbial inoculum, the additive, the antioxidant and peptidoglycan, the biological preparation capable of adjusting intestinal health of breeding pigeons and improving the immunity of the breeding pigeons is obtained, meanwhile, immune factors in pigeon milk can be improved, and the growth of young pigeons is promoted. The effects of improving the growth performance of the young pigeons and reducing the elimination rate of the breeding pigeons and the young pigeons can be achieved, so that economic benefits are improved, and wide application prospects are achieved.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The invention belongs to the field of aquatic bacteria detection, and relates to a fluorescent array sensor as well as a preparation method and application thereof. The fluorescent array sensor comprises a plurality of DNA aptamers, the DNA aptamers respectively target lipopolysaccharide, peptidoglycan and flagellin, the DNA aptamers of the target lipopolysaccharide are EA5 and LA27, the DNA aptamer of the target peptidoglycan is Antibac2, and the DNA aptamer of the target flagellin is Aptamer 3; the sequence of the EA5 is shown as SEQ ID NO.1, the sequence of the LA27 is shown as SEQ ID NO.2, the sequence of the Antibac2 is shown as SEQ ID NO.3, and the sequence of the Aptamer 3 is shown as SEQ ID NO.4. The invention further discloses a kit for detecting the content of the EA5, the LA27, the Antibac2 and the Aptamer. According to the present invention, the six bacteria can be simultaneously detected, the detection limit is low, the operation process is simple, and the detection accuracy can achieve 97.6%.

The invention discloses a mattress material based on modified fibers and a preparation method of the mattress material. The mattress material comprises nylon 66 fibers, low-melting-point fibers and the modified fibers. The low-melting-point fiber is ES bi-component parallel composite fiber composed of polypropylene and polyethylene, and the modified fiber is prepared by mixing a modified polymer and a modified filler to prepare a spinning solution and then carrying out electrostatic spinning and ultraviolet irradiation. Molecular chains of the modified polymer contain rich furan rings and thiophene rings, synthesis of bacterial cell walls can be destroyed, the furan is grafted with oximido, and a formed furan oxime ester unit can generate highly unstable and reactive free radicals, so that metal ions in cells are unbalanced to cause change of cell metabolism homeostasis. The lysozyme fixed on the modified filler can be hydrolyzed to form peptidoglycan of bacterial cell walls, so that the cell walls are broken, and the material has good antibacterial performance.

The invention relates to the first hydrolase that specifically digests the archaeal type of a peptidoglycan (PG), the major component of the cell wall of methanogens, named Talosaminidase. The enzyme consists of a signal peptide, several bacterial IG domains, a glycosyl hydrolase, several archaeal PG binding domains and a peptidase domain. The double enzymatic activity for the glycosyl hydrolase and the peptidase maximizes the lysis of archaeal cells with a cell wall of the archaeal PG-type. Engineering the enzyme by removing the signal peptide and bacterial IG domains and keeping only the archaeal PG binding, glycosyl hydrolase and peptidase made the enzyme easier to express and purify, without negatively influencing the enzymatic activity. Therefore, engineered Talosaminidase can be used to regulate populations of archaeal methanogens in the industrial, agricultural and medical settings, and consequently methane production.

The invention provides a postbiotic immunomodulatory preparation based on bifidobacterium longum CD11 and a preparation method of the postbiotic immunomodulatory preparation. Bifidobacterium longum CD11 is used to prepare a metagen. The immunocompetence of RAW264.7 macrophages can be promoted by thermal inactivation of bifidobacterium longum CD11 metabolites, cell walls, cell membranes and cell content metabolite components, and the cell wall component dose-dependently promotes the proliferation of the RAW264.7 macrophages and inhibits the relative expression of mRNA genes of inflammatory factors TNF-alpha and IL-1beta. In addition, a peptidoglycan (PGN) metagen from a cell wall component can remarkably inhibit relative expression of TNF-alpha and IL-1beta mRNA genes of RAW264.7 macrophages, so that inflammation is recovered to a health level (NC group level). Therefore, the bifidobacterium longum CD11 can be used as a potential immunomodulatory metabiotic strain.

The invention is directed to immunological compositions of one or more peptides containing epitopes of PGN, LTA and LPS molecules that induce an immunological response in a mammal, and to multiple antibodies that bind to these epitopes. Immunological compositions and antibodies disclosed herein can be used in the treatment and / or prevention of human health disorders such as bacterial sepsis, inflammation, cancers, tumors, inflammatory diseases and disorders, and neurodegenerative disorders such as, but not limited to Alzheimer's disease, frontotemporal dementia, chronic traumatic encephalopathy (CTE), Lewy body dementia and / or limbic predominant age-related TDP-43 encephalopathy (LATE).

The invention belongs to the field of food safety, and discloses application of cell wall lyase LysM15 in preparation of a bacillus cereus inhibitor. The cell wall lyase LysM15 has the functions of specifically recognizing the cell wall of the bacillus cereus and efficiently cracking the peptidoglycan layer of the bacillus cereus. The LysM15 not only can obviously inhibit the germination of bacillus cereus spores and remove biofilms of the bacillus cereus spores, but also can keep higher enzyme activity in a temperature range of 4-50 DEG C and in a high-salt environment. The lyase has good biocompatibility, growth and pollution of bacillus cereus in fresh beef can be effectively reduced, the potential of the lyase serving as a food preservative is shown, and a safe and efficient solution can be provided for food preservation.

The invention belongs to the technical field of molecular biology, and relates to a truncated fish peptidoglycan recognition protein and application thereof. The amino acid sequence of the truncated fish peptidoglycan recognition protein is as shown in SEQ ID NO. 1 in the table. Compared with a natural peptidoglycan recognition protein, the truncated fish peptidoglycan recognition protein provided by the invention has higher expression efficiency and higher yield in a prokaryotic expression system; in addition, the binding capacity with main components, namely peptidoglycan (PGN) and lipopolysaccharide (LPS), of cell walls of gram-positive bacteria and gram-negative bacteria is higher; the bacteriostasis and agglutination capacities on gram-positive bacteria and gram-negative bacteria are obviously improved. The characteristics show that the truncated fish peptidoglycan recognition protein has the development potential as an aquatic product immunopotentiator, and important support is provided for subsequent application research.

The invention discloses a sperm-friendly medical lubricant and a preparation method thereof. The lubricant comprises the following components: a water-based matrix, an active component, phosphate, a humectant, an osmotic pressure regulator and water, the active ingredients comprise small molecule peptidoglycan and alkaline polypeptide. The alkaline polypeptide is alkaline protein, can mildly improve the pH environment of the vagina and is beneficial to maintaining the activity of sperms, and the small molecule peptidoglycan has a good adhesion effect on vaginal epithelial cells, can be adhered to the vaginal epithelial cells and prevents the alkaline polypeptide from stimulating the vaginal inner wall; the small molecule peptidoglycan also can promote local immune regulation, enhance secretion of anti-inflammatory factors and immune response of macrophages, and improve the anti-infection ability of vagina; the composition of the two active substances can maintain sperm activity and protect and resist infection on the vagina at the same time, and is a lubricant which is friendly to the vagina and sperms.

The invention provides a primer pair, a kit and a method for detecting tilium pneumoniae, the primer pair comprises a forward primer and a reverse primer aiming at a peptidoglycan related lipoprotein (Pal) gene, and the Pal gene comprises a nucleotide sequence as shown in SEQ ID NO: 15. The length of the forward primer is 15-25 nucleotides, and the forward primer corresponds to a nucleotide sequence between the first site and the 33 site shown in SEQ ID NO: 15. The length of the retrograde primer is 15 to 30 nucleotides, and the retrograde primer corresponds to a nucleotide sequence between the 64th site and the 123 site of SEQ ID NO: 15. The invention also provides a kit containing the primer pair and used for detecting the lepidomonas pneumoniae, and a method for detecting the lepidomonas pneumoniae by using the primer pair.

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

This invention relates to the field of microbial technology and discloses a lactic acid bacteria postbiotic containing γ-aminobutyric acid (GABA) and its preparation method. It aims to solve the problems of GABA loss, damage to the active structure of the postbiotic cells, and insufficient synergistic utilization of GABA and GABA-containing lactic acid bacteria postbiotics during heat inactivation and drying processes. The method involves fermenting GABA-producing lactic acid bacteria, centrifuging to collect the supernatant and bacterial cells, desalting, removing impurities, and concentrating the supernatant to obtain a GABA-rich solution. The bacterial cell precipitate is washed, resuspended in a buffer solution containing calcium ions and trehalose, and allowed to stand. It is then adjusted to an acidic state to induce peptidoglycan conformation protection, followed by staged heat inactivation. After being combined with the GABA-rich solution and a composite protectant, it is dried at low temperature to obtain the GABA-containing lactic acid bacteria postbiotic. This provides a reliable technical solution for preparing highly active GABA-containing postbiotic products.

The invention discloses a condom added with sophocarpidine hyaluronic acid lubricating liquid and a preparation method of the condom, and belongs to the technical field of condom production. The core of the method is that after matrine is dissolved, the matrine is added into hyaluronic acid lubricating liquid for the condoms, the hyaluronic acid lubricating liquid containing the matrine is prepared through stirring and mixing, the concentration of the matrine in the hyaluronic acid lubricating liquid is 7.7-25.0 mg / g, when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms, and when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms. And preparing the condom added with the sophocarpidine-containing hyaluronic acid lubricating liquid. According to the condom, inflammation generated after vaginal epithelial cells are stimulated by bacterial structural components is improved through matrine, and the common pathogenic bacteria such as staphylococcus aureus, escherichia coli, enterococcus faecalis and streptococcus agalactiae of vaginitis are inhibited in the using process; and the up-regulation level of NF-kB phosphorylation generated after lipopolysaccharide and peptidoglycan stimulate vaginal epithelial cells is relieved.

The present invention provides a probiotic composition for inhibiting Helicobacter pylori. Lacticaseibacillus rhamnosus was preserved in Guangdong Microbial Culture Collection Center on 11 April 2024, and the accession number is GDMCC NO: 64513. Lactiplantibacillusplantarum was preserved in Guangdong Microbial Culture Collection Center on 11 April 2024, and the accession number is GDMCC NO: 64514. The advantage is: peptidoglycans extracted from the Lacticaseibacillus rhamnosus and the Lactiplantibacillusplantarum can effectively inhibit Helicobacter pylori. The combination of peptidoglycans from the two strains exhibits an enhanced ability to inhibit Helicobacter pylori compared with the same concentration of peptidoglycan from a single strain.

The invention relates to the technical field of biological medicine, and discloses application of cell wall component peptidoglycan in activating lysosome and delaying healthy aging. The invention finds that the peptidoglycan, which is a bacterial cell wall component, can improve lysosome activity. Experiments prove that the peptidoglycan serving as the bacterial cell wall component can improve lysosome activity so as to delay animal aging, so that the peptidoglycan serving as the bacterial cell wall component can be used for preparing lysosome activators or medicines for delaying healthy aging.

The invention relates to the field of agriculture, discloses a Citrus Huanglongbing prevention and control method and system based on a composite lysozyme preparation, and provides a composite lysozyme synergistic prevention and control technology for solving the problems that pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, ecological balance is damaged and the like due to existing chemical pesticides. The preparation comprises the following components in percentage by weight: 30-50% of egg white lysozyme, 25-35% of chitosanase, 20-30% of a plant-source synergist (a mixture of citrus essential oil and tea polyphenol) and 3-8% of trehalose. The pathogen-medium synchronous targeting is realized through a triple action mechanism: (1) lysozyme is used for cracking a phloem bacitracin peptidoglycan cell wall; (2) degrading the chitin layer of the middle intestine of the phylloxera (the fatality rate is greater than or equal to 91.5%) by using the chitosanase; 3, the citrus essential oil interferes with smell localization of the phylloxera, intelligent prevention and control are achieved through a three-level prevention and control system, efficiency and labor are saved, and the scheme is remarkably superior to a traditional

This invention discloses a poly-T double-stranded chimeric DNA-copper nanoparticle and its synthesis method, belonging to the field of DNA-metal nanomaterials technology. This invention uses a poly-T double-stranded DNA chimeric structure as a template to construct a DNA tetrahedron. Copper ions are reduced in situ with ascorbic acid to prepare DNA-copper nanoparticles exhibiting both fluorescence and peroxidase-like activity. The DNA tetrahedron template is formed by the self-assembly of four single-stranded DNA strands, containing six double-stranded edges and four single-stranded vertices. Two of the single strands are poly-T sequences, and two are aptamer sequences targeting bacterial cell wall peptidoglycan. The prepared DNA-copper nanoparticles emit characteristic fluorescence under 340 nm excitation and exhibit peroxidase-like activity. This material is used for bacterial detection and eradication, with minimum inhibitory concentrations (MICs) of 25 nM and 5 nM against *Escherichia coli* and methicillin-resistant *Staphylococcus aureus*, respectively. 3 Up to 10 8 The ability to detect Escherichia coli within the CFU / L concentration range demonstrates its potential for immediate clinical antibacterial applications.

The application discloses a kind of peptidoglycan recognition protein-D, preparation method and application, belong to biological medicine technical field.The application obtains natural peptidoglycan recognition protein-D from tussah by protein separation and purification technology, realizes the expression of peptidoglycan recognition protein-D and its derivative or analogue or partial fragment gene in host cell after resolving its primary structure (gene and protein) using genetic engineering technology, and the antibody of purified recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment is obtained by immunizing animal.The natural, recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment and its antibody of the application can be widely used for prevention, detection diagnosis, treatment and other biological medicine fields of microorganism, microorganism related molecular pattern.

The invention discloses a method for increasing the yield of extracellular vesicles of lactococcus lactis and application of the method, and belongs to the technical field of biology. The lysozyme and the ampicillin are used for weakening a lactococcus lactis peptidoglycan layer, so that the yield of the natural bacterial extracellular vesicles of the lactococcus lactis is increased. The technological process is simple, and is suitable for industrial amplification and standardized production; the vesicle yield is high, the amplification effect and the purification effect are good, and the method can be used for large-scale preparation of vesicles. The lactococcus lactis extracellular vesicles have the adjuvant efficacy of stimulating the maturation of dendritic cells and the tropism of targeting solid tumor enrichment, and can be used as an immunopotentiator or a sending carrier of vaccines and nano-drugs.

The present application relates to the field of biotechnology, in particular to a kind of peptide endo-enzyme modified strain and its construction method and application, the strain is gene knockout clostridium saccharolyticum;The function of the peptide endo-enzyme in the gene knockout clostridium saccharolyticum is inactivated.The strain is in clostridium saccharolyticum Knockout the peptide endo-enzyme coding gene that has hydrolyzed bacterial cell wall peptidoglycan structure, thereby reducing cell autolysis activity, increasing anaerobic fermentation active cell density, relieving cell autolysis occurs, reducing the dissolution of intracellular protein and other contents, to strengthen solvent synthesis capacity such as ethanol, n-butanol, improve the concentration and yield of anaerobic fermentation solvent product, and significantly enhance the stress tolerance of clostridium saccharolyticum to n-butanol.

The present application belongs to the field of fermentation engineering and biomass resource utilization, and particularly relates to preparation of a composite nanofiber membrane by utilizing fermentation bacteria after fermentation production is completed. In the present application, the fermentation bacteria are subjected to chemical lysis to release biological macromolecules. The lysis solution of the bacteria after lysis contains biological macromolecules such as proteins, nucleic acids, peptidoglycan and flocculating agent chitosan. Then, the bacteria lysis solution is blended with starch and PEO, and a composite nanofiber is prepared by electrospinning, so as to realize productized application of the waste fermentation bacteria.

The invention relates to the technical field of antibiotic enzymes, and provides a peptide fusion antibiotic enzyme, the peptide fusion antibiotic enzyme comprises a catalytic domain LysS23, a cell-penetrating peptide and a connecting peptide, the catalytic domain LysS23 comprises an amino acid sequence represented by SEQ ID NO: 1, the cell-penetrating peptide comprises an amino acid sequence represented by SEQ ID NO: 2, and the connecting peptide comprises an amino acid sequence represented by SEQ ID NO: 3. Or an amino acid sequence which is obtained by substitution / deletion / addition of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1 and has more than 98% of identity is obtained by substitution / deletion / addition of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1. The catalytic domain LysS23 in the peptide fusion antibiotic enzyme has peptidoglycan lysis activity, the cell-penetrating peptide exerts a cell-penetrating function and has an outer membrane destruction effect, the cell-penetrating peptide acts on an extracellular membrane and helps the catalytic domain LysS23 to smoothly reach the peptidoglycan position of a cell wall so as to quickly exert the lysis effect, and the connecting peptide maintains the integrity of the structures and functions of the cell-penetrating peptide and the catalytic domain. Meanwhile, the antibacterial activity of the peptide fusion antibiotic enzyme is ensured. The peptide fusion antibiotic enzyme provided by the invention can directly act on common gram-negative bacteria without depending on the addition of a chemical outer membrane penetrant, exerts a good antibacterial effect, and has a very good application value.

The present disclosure relates to a method of diagnosing Lyme disease in a subject comprising measuring the level of B. burgdorferi peptidoglycan or the level of an antibody that specifically binds to B. burgdorferi peptidoglycan (“anti-peptidoglycan agent”). The present disclosure also relates to a method of treating a Lyme disease in a subject in need thereof comprising administering to the subject an antagonist against B. burgdorferi peptidoglycan (e.g., an anti-peptidoglycan antibody or a peptidoglycan-specific hydrolase). Antagonists (e.g., anti-peptidoglycan antibodies) suitable for the present methods are also disclosed.

This invention discloses a biological agent suitable for squab growth, its preparation method, and its application, belonging to the field of feed technology. It comprises the following components by weight: 8-40 parts of microbial agent, 1-10 parts of additive, 1-10 parts of antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite. The microbial agent includes *Bifidobacterium animalis*, *Lactobacillus acidophilus*, *Lactobacillus paracasei*, and *Lactobacillus reuteri*. The additive includes at least one of yeast β-glucan and astragalus polysaccharide. The biological agent for squab growth of this application uses montmorillonite as a base material. Under the combined action of the microbial agent, additive, antioxidant, and peptidoglycan, it can regulate the intestinal health of breeding pigeons, improve their immunity, and increase immune factors in pigeon milk. This can improve the growth performance of squabs, reduce the culling rate of both breeding and squabs, thereby increasing economic benefits and showing broad application prospects.

The invention relates to a method for preparing mushroom cell wall glycosaminoglycan with free radical scavenging activity by a compound enzyme method. According to the method, based on the characteristic that the cell wall of the edible fungi is mainly composed of hard and indigestible peptidoglycan, papain, beta-glucanase, pectinase and bromelain are utilized to carry out targeted enzymolysis on peptide bonds and glucosidic bonds of peptidoglycan, and-N + H3 and-COO-of glycosaminoglycan are exposed to increase the activity potential; and separating by using high-concentration ethanol to obtain the mushroom glycosaminoglycan with the free radical scavenging function. The method is suitable for extracting the mushroom cell wall glycosaminoglycan, and has the characteristics of rich raw material sources, high product yield and functional activity of the product. Belongs to the fields of agriculture, health and food.