33 results about "Peptidoglycan" patented technology

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

The application discloses a chimeric endolysin Lys009-Lys009CBD and engineering and application thereof, and belongs to the field of genetic engineering and protein engineering. The application constructs a chimeric endolysin Lys009-Lys009CBD with high activity, and the peptidoglycan hydrolysis activity of the chimeric endolysin Lys009-Lys009CBD on Pseudomonas aeruginosa is 5.30 times that of natural endolysin Lys009 and 27.98 times that of hen egg white lysozyme HEWL. A transmembrane peptide P81417 is further fused to perform transmembrane modification, and an engineering endolysin P81417-Lys009-Lys009CBD is obtained. The engineering endolysin can efficiently lyse Pseudomonas aeruginosa, Acinetobacter baumannii and the like, and has excellent thermal stability. The application enriches the resource library of high-activity endolysins, provides important experimental basis and technical support for developing an anti-drug-resistant bacteria treatment strategy, and also provides a new selection for clinical treatment of multiple drug-resistant bacteria infection.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The invention belongs to the field of aquatic bacteria detection, and relates to a fluorescent array sensor as well as a preparation method and application thereof. The fluorescent array sensor comprises a plurality of DNA aptamers, the DNA aptamers respectively target lipopolysaccharide, peptidoglycan and flagellin, the DNA aptamers of the target lipopolysaccharide are EA5 and LA27, the DNA aptamer of the target peptidoglycan is Antibac2, and the DNA aptamer of the target flagellin is Aptamer 3; the sequence of the EA5 is shown as SEQ ID NO.1, the sequence of the LA27 is shown as SEQ ID NO.2, the sequence of the Antibac2 is shown as SEQ ID NO.3, and the sequence of the Aptamer 3 is shown as SEQ ID NO.4. The invention further discloses a kit for detecting the content of the EA5, the LA27, the Antibac2 and the Aptamer. According to the present invention, the six bacteria can be simultaneously detected, the detection limit is low, the operation process is simple, and the detection accuracy can achieve 97.6%.

The invention discloses a mattress material based on modified fibers and a preparation method of the mattress material. The mattress material comprises nylon 66 fibers, low-melting-point fibers and the modified fibers. The low-melting-point fiber is ES bi-component parallel composite fiber composed of polypropylene and polyethylene, and the modified fiber is prepared by mixing a modified polymer and a modified filler to prepare a spinning solution and then carrying out electrostatic spinning and ultraviolet irradiation. Molecular chains of the modified polymer contain rich furan rings and thiophene rings, synthesis of bacterial cell walls can be destroyed, the furan is grafted with oximido, and a formed furan oxime ester unit can generate highly unstable and reactive free radicals, so that metal ions in cells are unbalanced to cause change of cell metabolism homeostasis. The lysozyme fixed on the modified filler can be hydrolyzed to form peptidoglycan of bacterial cell walls, so that the cell walls are broken, and the material has good antibacterial performance.

The invention is directed to immunological compositions of one or more peptides containing epitopes of PGN, LTA and LPS molecules that induce an immunological response in a mammal, and to multiple antibodies that bind to these epitopes. Immunological compositions and antibodies disclosed herein can be used in the treatment and / or prevention of human health disorders such as bacterial sepsis, inflammation, cancers, tumors, inflammatory diseases and disorders, and neurodegenerative disorders such as, but not limited to Alzheimer's disease, frontotemporal dementia, chronic traumatic encephalopathy (CTE), Lewy body dementia and / or limbic predominant age-related TDP-43 encephalopathy (LATE).

The invention belongs to the technical field of molecular biology, and relates to a truncated fish peptidoglycan recognition protein and application thereof. The amino acid sequence of the truncated fish peptidoglycan recognition protein is as shown in SEQ ID NO. 1 in the table. Compared with a natural peptidoglycan recognition protein, the truncated fish peptidoglycan recognition protein provided by the invention has higher expression efficiency and higher yield in a prokaryotic expression system; in addition, the binding capacity with main components, namely peptidoglycan (PGN) and lipopolysaccharide (LPS), of cell walls of gram-positive bacteria and gram-negative bacteria is higher; the bacteriostasis and agglutination capacities on gram-positive bacteria and gram-negative bacteria are obviously improved. The characteristics show that the truncated fish peptidoglycan recognition protein has the development potential as an aquatic product immunopotentiator, and important support is provided for subsequent application research.

The invention provides a primer pair, a kit and a method for detecting tilium pneumoniae, the primer pair comprises a forward primer and a reverse primer aiming at a peptidoglycan related lipoprotein (Pal) gene, and the Pal gene comprises a nucleotide sequence as shown in SEQ ID NO: 15. The length of the forward primer is 15-25 nucleotides, and the forward primer corresponds to a nucleotide sequence between the first site and the 33 site shown in SEQ ID NO: 15. The length of the retrograde primer is 15 to 30 nucleotides, and the retrograde primer corresponds to a nucleotide sequence between the 64th site and the 123 site of SEQ ID NO: 15. The invention also provides a kit containing the primer pair and used for detecting the lepidomonas pneumoniae, and a method for detecting the lepidomonas pneumoniae by using the primer pair.

This invention relates to the field of nonwoven fabric preparation technology, specifically to a biochemically modified antibacterial nonwoven fabric and its preparation method. This invention modifies nano-zinc oxide and nano-titanium dioxide using the silane coupling agent KH-570, improving the dispersibility of the nanoparticles. Under light or contact conditions, the nanoparticles release reactive oxygen species to disrupt bacterial cell membranes and inhibit their metabolism, thereby giving the antibacterial modified polypropylene masterbatch stable antibacterial properties. Furthermore, this invention stabilizes the loading through a polydopamine coating, thus stabilizing the antibacterial properties of lysozyme. Lysozyme specifically hydrolyzes the peptidoglycan layer of bacterial cell walls, leading to bacterial lysis. Thiourea dioxide, as a reducing agent, maintains lysozyme activity, preventing oxidative inactivation, while simultaneously inhibiting bacterial metabolic enzyme activity. Through the synergistic effect of inorganic and biological dual-effects, this invention can cover Gram-positive bacteria, Gram-negative bacteria, and fungi, thereby improving the antibacterial properties of the nonwoven fabric to a certain extent.

This invention relates to the field of microbial technology and discloses a lactic acid bacteria postbiotic containing γ-aminobutyric acid (GABA) and its preparation method. It aims to solve the problems of GABA loss, damage to the active structure of the postbiotic cells, and insufficient synergistic utilization of GABA and GABA-containing lactic acid bacteria postbiotics during heat inactivation and drying processes. The method involves fermenting GABA-producing lactic acid bacteria, centrifuging to collect the supernatant and bacterial cells, desalting, removing impurities, and concentrating the supernatant to obtain a GABA-rich solution. The bacterial cell precipitate is washed, resuspended in a buffer solution containing calcium ions and trehalose, and allowed to stand. It is then adjusted to an acidic state to induce peptidoglycan conformation protection, followed by staged heat inactivation. After being combined with the GABA-rich solution and a composite protectant, it is dried at low temperature to obtain the GABA-containing lactic acid bacteria postbiotic. This provides a reliable technical solution for preparing highly active GABA-containing postbiotic products.

The invention discloses a condom added with sophocarpidine hyaluronic acid lubricating liquid and a preparation method of the condom, and belongs to the technical field of condom production. The core of the method is that after matrine is dissolved, the matrine is added into hyaluronic acid lubricating liquid for the condoms, the hyaluronic acid lubricating liquid containing the matrine is prepared through stirring and mixing, the concentration of the matrine in the hyaluronic acid lubricating liquid is 7.7-25.0 mg / g, when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms, and when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms. And preparing the condom added with the sophocarpidine-containing hyaluronic acid lubricating liquid. According to the condom, inflammation generated after vaginal epithelial cells are stimulated by bacterial structural components is improved through matrine, and the common pathogenic bacteria such as staphylococcus aureus, escherichia coli, enterococcus faecalis and streptococcus agalactiae of vaginitis are inhibited in the using process; and the up-regulation level of NF-kB phosphorylation generated after lipopolysaccharide and peptidoglycan stimulate vaginal epithelial cells is relieved.

This invention discloses a poly-T double-stranded chimeric DNA-copper nanoparticle and its synthesis method, belonging to the field of DNA-metal nanomaterials technology. This invention uses a poly-T double-stranded DNA chimeric structure as a template to construct a DNA tetrahedron. Copper ions are reduced in situ with ascorbic acid to prepare DNA-copper nanoparticles exhibiting both fluorescence and peroxidase-like activity. The DNA tetrahedron template is formed by the self-assembly of four single-stranded DNA strands, containing six double-stranded edges and four single-stranded vertices. Two of the single strands are poly-T sequences, and two are aptamer sequences targeting bacterial cell wall peptidoglycan. The prepared DNA-copper nanoparticles emit characteristic fluorescence under 340 nm excitation and exhibit peroxidase-like activity. This material is used for bacterial detection and eradication, with minimum inhibitory concentrations (MICs) of 25 nM and 5 nM against *Escherichia coli* and methicillin-resistant *Staphylococcus aureus*, respectively. 3 Up to 10 8 The ability to detect Escherichia coli within the CFU / L concentration range demonstrates its potential for immediate clinical antibacterial applications.

The application discloses a kind of peptidoglycan recognition protein-D, preparation method and application, belong to biological medicine technical field.The application obtains natural peptidoglycan recognition protein-D from tussah by protein separation and purification technology, realizes the expression of peptidoglycan recognition protein-D and its derivative or analogue or partial fragment gene in host cell after resolving its primary structure (gene and protein) using genetic engineering technology, and the antibody of purified recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment is obtained by immunizing animal.The natural, recombinant peptidoglycan recognition protein-D and its derivative or analogue or partial fragment and its antibody of the application can be widely used for prevention, detection diagnosis, treatment and other biological medicine fields of microorganism, microorganism related molecular pattern.

The present application belongs to the field of fermentation engineering and biomass resource utilization, and particularly relates to preparation of a composite nanofiber membrane by utilizing fermentation bacteria after fermentation production is completed. In the present application, the fermentation bacteria are subjected to chemical lysis to release biological macromolecules. The lysis solution of the bacteria after lysis contains biological macromolecules such as proteins, nucleic acids, peptidoglycan and flocculating agent chitosan. Then, the bacteria lysis solution is blended with starch and PEO, and a composite nanofiber is prepared by electrospinning, so as to realize productized application of the waste fermentation bacteria.

The present disclosure relates to a method of diagnosing Lyme disease in a subject comprising measuring the level of B. burgdorferi peptidoglycan or the level of an antibody that specifically binds to B. burgdorferi peptidoglycan (“anti-peptidoglycan agent”). The present disclosure also relates to a method of treating a Lyme disease in a subject in need thereof comprising administering to the subject an antagonist against B. burgdorferi peptidoglycan (e.g., an anti-peptidoglycan antibody or a peptidoglycan-specific hydrolase). Antagonists (e.g., anti-peptidoglycan antibodies) suitable for the present methods are also disclosed.

This invention discloses a biological agent suitable for squab growth, its preparation method, and its application, belonging to the field of feed technology. It comprises the following components by weight: 8-40 parts of microbial agent, 1-10 parts of additive, 1-10 parts of antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite. The microbial agent includes *Bifidobacterium animalis*, *Lactobacillus acidophilus*, *Lactobacillus paracasei*, and *Lactobacillus reuteri*. The additive includes at least one of yeast β-glucan and astragalus polysaccharide. The biological agent for squab growth of this application uses montmorillonite as a base material. Under the combined action of the microbial agent, additive, antioxidant, and peptidoglycan, it can regulate the intestinal health of breeding pigeons, improve their immunity, and increase immune factors in pigeon milk. This can improve the growth performance of squabs, reduce the culling rate of both breeding and squabs, thereby increasing economic benefits and showing broad application prospects.

This invention provides an application of *Lactobacillus plantarum* peptidoglycan in the preparation of uric acid-lowering products, relating to the biomedical field. This *Lactobacillus plantarum* peptidoglycan is derived from *Lactobacillus plantarum* (accession number CGMCC No. 25306). Lactobacillus plantarum Extracted from YC, its chemical structure is A1γ. This plant lactobacillus peptidoglycan can regulate uric acid homeostasis through multiple synergistic mechanisms: inhibiting hepatic XOD activity to reduce uric acid production; bidirectionally regulating renal and intestinal uric acid transport proteins (upregulating ABCG2 / OAT1, downregulating URAT1 / GLUT9) to promote excretion; and exerting systemic anti-inflammatory and hepatoprotective effects. This plant lactobacillus peptidoglycan has a significant uric acid-lowering effect without hepatotoxicity or nephrotoxicity, and its safety is superior to allopurinol. This invention provides a novel core material basis and solution for developing multi-pathway, highly safe uric acid-lowering products.

The invention discloses application of Pediococcus pentosaceus PPSJ001 or peptidoglycan of the Pediococcus pentosaceus PPSJ001 in preparation of a product for preventing or treating intestinal inflammatory diseases, and the Pediococcus pentosaceus PPSJ001 is Pediococcus pentosaceus. The invention further discloses a preparation method of the Pediococcus pentosaceus PPSJ001 and a preparation method of the Pediococcus pentosaceus PPSJ001. According to the application research of the pediococcus pentosaceus PPSJ001 and peptidoglycan thereof in DSS-induced acute and chronic colitis mouse models, prophylactic or therapeutic gavage can obviously reduce the inflammation degree, relieve weight loss, restore the colon length and improve pathological damage of colon tissue. The pediococcus pentosaceus PPSJ001 can be used for promoting the expression of tight junction proteins ZO-1 and Occludin and mucoprotein Muc2 and improving the barrier function of intestinal mucosa. The key functional component is cell wall peptidoglycan, and the peptidoglycan can remarkably relieve the colitis disease in vivo, promote polarization of macrophages to a CD206 + M2 type in vitro and improve expression of anti-inflammatory related genes Arg1. Based on the unique functions, the pediococcus pentosaceus PPSJ001 or peptidoglycan thereof can be coordinated or complemented with other micro-ecological regulation means, and has a wide application prospect in medicine preparation.

The present disclosure provides peptidoglycan precursors and the use thereof in the study of binding between antibiotic and peptidoglycan. Specifically, the peptidoglycan precursors disclosed herein are used in a method for the screening of peptidoglycan-targeting antibiotics and identifying the binding moiety on a peptidoglycan.

This invention discloses an activated water-soluble photodynamic antibacterial agent based on bacterial bioorthogonal labeling and its application. The activated water-soluble photodynamic antibacterial agent of this invention contains hydrophilic groups, which can prevent uptake by normal cells, thereby reducing phototoxicity to normal tissues. In addition, the activated water-soluble photodynamic antibacterial agent of this invention is modified with bioorthogonal reactive groups that can quench the fluorescence and ROS generation capabilities of photosensitizers. This allows for rapid click chemistry reactions with active groups inserted into peptidoglycans during bacterial metabolism, achieving specific labeling and in-situ activation of the photosensitizer on the bacterial surface. Under near-infrared light irradiation, the photodynamic antibacterial agent specifically modified on the bacterial surface can generate a large amount of reactive oxygen species, selectively killing bacteria while avoiding damage to normal tissues, exhibiting broad-spectrum antibacterial activity and showing promising application prospects in photodynamic antibacterial therapy.

The present invention relates to an isolated muropeptide of formula (I) GlcNAc-MurNAc-X1-Glu-mDAP-(Ala)Y1-(X2)Y2 as well as peptidoglycans (PG) comprising it and to a composition comprising at least one muropeptide and / or at least one PG and to the implementation of the muropeptide, peptidoglycan or composition in the prevention and / or treatment, in an individual in need thereof, of an immune disorder selected from the group consisting of an inflammatory disorder, graft-versus-host disease (GVHD) and graft rejection; or of an infection; or of a metabolic disorder or of a cancer.

The invention relates to the field of feeds, in particular to a full-stage rabbit feed based on golden probiotic elements and a preparation method of the full-stage rabbit feed. The feed comprises a coarse material, prebiotics and probiotics, the prebiotics comprise 5-15 g / kg of fructooligosaccharide and 10-25 g / kg of chicory root powder on the basis of each kg of the whole-stage rabbit feed; the probiotics comprise 1 * 10 < 9 >-3 * 10 < 9 > CFU / Kg of bacillus subtilis, 0.8 * 10 < 9 >-1 * 10 < 9 > CFU / Kg of bacillus licheniformis and 1 * 10 < 9 >-3 * 10 < 9 > CFU / Kg of inactivated lactobacillus acidophilus. All the raw materials have a synergistic effect and are matched with a peptidoglycan enriched fermented soybean meal carrier (the mass ratio is 1: 2), so that (1) active bacterium digestion and mucous membrane repair and inactivated bacterium immune activation are realized; (2) the double-chain prebiotics cover near and far ends of intestinal tracts; and (3) the retention rate of the carrier protected peptidoglycan is greater than or equal to 94.7%, and the problem of high-temperature inactivation is solved.

The invention relates to the technical field of molecular biology and immunity, in particular to urechis unicinctus peptidoglycan recognition protein and a preparation method and application thereof. The amino acid sequence of the urechis unicinctus peptidoglycan recognition protein is as shown in SEQ ID No. 1. The peptidoglycan recognition protein is a recombinant protein obtained by expression in escherichia coli. The recombinant protein can be widely combined and agglutinated with various gram-positive bacteria and gram-negative bacteria, and can promote the removal of pathogenic bacteria in urechis unicinctus. The recombinant peptidoglycan recognition protein can be used for preparing a bacterial agglutination preparation, an anti-bacterial infection preparation and the like, and is especially suitable for prevention and control of aquaculture pathogenic bacteria.

The invention provides a collagen dressing for promoting wound healing and a preparation method thereof. The dressing is prepared from the following raw materials in parts by weight: 20 to 26 parts of collagen peptide, 17 to 21 parts of mussel extract, 6 to 9 parts of keratin, 6 to 9 parts of sodium alginate, 4 to 7 parts of peptidoglycan, 1 to 3 parts of polypeptide, 18 to 23 parts of polysaccharide, 16 to 20 parts of compound plant extract, 8 to 13 parts of sodium hyaluronate, 7 to 11 parts of gelatin, 8 to 12 parts of carbomer, 7 to 10 parts of glycerinum, 0.002 to 0.004 part of preservative and 66 to 80 parts of water. The collagen peptide, the mussel extract, the keratin, the peptidoglycan, the polypeptide, the polysaccharide, the composite plant extract, the sodium hyaluronate and other raw materials are reasonably matched and used, so that a protective layer can be formed on a wound surface, exudate of the wound is effectively absorbed, good anti-inflammatory and antibacterial effects can be achieved, invasion of external harmful germs can be effectively resisted, and the wound healing effect is good. The cell activity can also be improved, wound skin cell regeneration is promoted, wound healing is helped, and the wound healing time is effectively shortened.

This invention discloses a method for preparing Fe3O4-based magnetic nanomaterials. The nanomaterials use iron(III) oxide (Fe3O4) as a core, and phenylenediamine and copper are co-modified onto their surface via a stirring method. On one hand, the phenylenediamine surface is rich in functional groups, which is beneficial for subsequent functionalization modification; its aniline-like structure can effectively enhance the photothermal effect of the nanomaterials. On the other hand, after modification with copper, the nanomaterials have a high affinity for bacteria, achieving rapid enrichment and efficient capture of pathogenic bacteria through electrostatic and metal coordination interactions. Furthermore, the photothermal effect generated by this magnetic nanomaterial under infrared light irradiation can synergistically enhance its enzyme-like activity, achieving highly efficient sterilization. Most importantly, this material can also efficiently remove harmful products generated after bacterial lysis, including peptidoglycan and lipopolysaccharide. This invention effectively solves the technical defects of traditional antibacterial materials, such as limited functionality and inability to remove toxic lysis products, demonstrating strong practicality and broad application prospects.

The present disclosure relates to a method of diagnosing Lyme disease in a subject comprising measuring the level of B. burgdorferi peptidoglycan or the level of an antibody that specifically binds to B. burgdorferi peptidoglycan (“anti-peptidoglycan agent”). The present disclosure also relates to a method of treating a Lyme disease in a subject in need thereof comprising administering to the subject an antagonist against B. burgdorferi peptidoglycan (e.g., an anti-peptidoglycan antibody or a peptidoglycan-specific hydrolase). Antagonists (e.g., anti-peptidoglycan antibodies) suitable for the present methods are also disclosed.

The application discloses a bacteriostatic composition and application thereof in hand sanitizer, and the bacteriostatic composition comprises the following components in percentage by mass: lysozyme 3-5%, enzyme protection system 4-8%, plant bacteriostatic extract 8-12%, biological matrix carrier 10-15%, stable protection system 5-8%, and the balance is water. The bacteriostatic composition can form a protective layer with good biocompatibility on the skin surface through a three-dimensional network structure formed by a carboxymethyl chitosan-sodium alginate compound, and can significantly prolong the skin retention time of lysozyme and plant bacteriostatic active ingredients. In the application, lysozyme and plant bacteriostatic ingredients are used to form a multiple bacteriostatic mechanism: lysozyme specifically hydrolyzes peptidoglycan in the bacterial cell wall, and active ingredients in the plant bacteriostatic extract destroy the bacterial cell membrane and inhibit bacterial metabolism. The double attack mechanism has a stronger bacteriostatic effect than a single component, and can prevent bacteria from developing drug resistance.

The invention relates to the technical field of pesticides, in particular to high-toxicity broad-spectrum metarhizium anisopliae for targeted inhibition of insect peptidoglycan recognition protein as well as a construction method and application of the metarhizium anisopliae. The coding gene disclosed by the invention can target an immune gene peptidoglycan recognition protein LB, so that the transcriptional level of the peptidoglycan recognition protein LB in an insect body is inhibited. The recombinant entomopathogenic fungus disclosed by the invention can be used for improving the insect killing efficiency, shortening the half lethal time by nearly 30%, and is harmless to the environment, good in biological safety and non-toxic to human beings.

1. Name of the designed product: packaging set (red ginseng double flower peptidoglycan drink). 2. Use of the designed product: for product packaging. 3. Design points of the designed product: in the pattern. 4. Picture or photo best indicating the design points: main view of set 3. 5. The designed product is a thin product, omitting the left view, right view, top view, and bottom view of set 1. 6. Other circumstances needing explanation: The designed product is a set product, so it is used and sold as a set. Set 1 is an inner packaging bag, set 2 is an inner packaging box, and set 3 is an outer packaging box. Set 1 is packed into set 2 and then into set 3 for sale and use.