12 results about "Amidase" patented technology

The present application relates to the technical field of recombinant protein technology, and particularly relates to a high-salt-tolerant amidase, gene, vector, recombinant bacteria and application, wherein the amino acid sequence of the amidase is shown as SEQ ID NO. 1. The amidase has extremely high salt tolerance and good ethanol tolerance. The recombinant amidase prepared by the present application lays a foundation for future realization of elimination of ethyl carbamate in high-salt fermented food and alcoholic beverages and realization of industrial application of ethyl carbamate hydrolytic enzyme, and has great economic and social benefits.

This invention relates to the field of microbial technology, specifically to a method for the production and extraction of N-acetylmuroic acid-L-alanine amidase via microbial fermentation. The method for preparing N-acetylmuroic acid-L-alanine amidase provided by this invention utilizes *Clostridium beijerinckii* XH0906 for fermentation to produce N-acetylmuroic acid-L-alanine amidase. As the main extracellular protein of this bacterium, N-acetylmuroic acid-L-alanine amidase has a high yield and is beneficial for subsequent separation and purification. This method has advantages such as simplicity, efficiency, and low cost, providing an effective method for the preparation of N-acetylmuroic acid-L-alanine amidase, and has good application prospects in the fields of microbial food, biomedicine, and antibacterial applications.

The present application relates to a beta-lactamase inhibitor intermediate co-crystal or salt, a preparation method and application thereof, and belongs to the technical field of pharmaceutical chemical engineering. The structural formula is as follows: R2 represents benzyl or allyl; R3 represents an alkylamine group with a protective group, a cycloalkylamine group, an aromatic amine group, and a derivative amine group. The preparation method comprises the following steps: dissolving compound I in a solvent A, adding a base solution to react under the condition of less than 0℃, then adding an acid to adjust the pH to be acidic, adding a solvent B to extract the product, separating the layers, and leaving the organic phase; and then adding a beta-lactamase inhibitor side chain R3H to react. The beta-lactamase inhibitor is prepared by using the intermediate co-crystal or the new salt, the purity is greater than 99.90%, the pharmaceutical quality standard of the crude drug is met, the process operation is safe, simple, convenient, green and environment-friendly; the atom economy, the selectivity, the purity and the yield of each step reaction are high, and the production can be enlarged.

This invention provides an amidase mutant and its application in the preparation of ( S The application of )-fluopyrazopyr in aniline belongs to the fields of genetic engineering and enzyme catalysis technology. Compared with the amidase shown in SEQ ID No. 1, the amidase mutant of this invention has a mutation at position 109 (alanine) to valine and / or position 232 (leucine) to arginine. Compared with the wild-type amidase, the amidase mutant of this invention has a higher optimum temperature and thermal stability, and can catalyze the enzymatic reaction of racemic substrate M-1 (Formula I) in an aqueous phase to generate the key chiral intermediate (…). S )-M-2 (Formula II), further reacted with compound M-3 (Formula III), to efficiently prepare ( S The method for preparing 1-fluopyrazoline is characterized by mild reaction conditions, simple operation, and promising prospects for industrial application.

Disclosed herein are processes for the preparation of fused heteroaryl dihydro pyrimidine compounds, or salts or stereoisomers thereof, which are useful for the treatment and prophylaxis of hepatitis B virus infections using compounds of formula IX or stereoisomers thereof,wherein a disclosed process comprisesa. reacting a compound formula IIwith hydrogen in the presence of a solvent and a palladium catalyst or a platinum catalyst, to form a compound of formula III:b. reacting the compound of formula III, or a salt or stereoisomer thereof, with a hydrolase selected from the group consisting of an amidase and a peptidase, or a mixture thereof, to form a compound of formula I:or a salt or stereoisomer thereof,c. protecting the compound of formula I, or a salt or stereoisomer thereof, with an amino protecting group (PG) selected from the group consisting of di-tert-butyl dicarbonate (Boc2O) and 2-(tert-butoxycarbonyloxyimino)-2-phenylacetonitrile, to form a compound of formula IV:d. reacting the compound of formula IV, or a salt or stereoisomer thereof, with a compound of the following formula: —R7—NH2 in the presence of a base and the coupling agent, carbonyldiimidazole (CDI), to form a compound of formula V:or a salt or stereoisomer thereof,e. reacting the compound of formula V above, or a salt or stereoisomer thereof, with oxalyl chloride to form a compound of formula VIf. reacting the compound of formula VI above, or a stereoisomer thereof, with a reducing agent selected from the group consisting of BH3·THF and NaBH4, to form a compound of formula VII:g. deprotecting the compound of formula VII above, or a stereoisomer thereof, with concentrated HCl in methyl isobutyl ketone (MIBK), to form a compound of formula IX:

Provided are a method for biomanufacturing an organosilicon hydrogel by means of multi-enzyme cascade and the use thereof. The method comprises the following steps: S1, using a biogas slurry from cassava-based fuel ethanol production as a raw material, and mixing the biogas slurry with Bacillus aryabhattai; S2, purifying the biogas slurry via the production of amidase and unsaturated lipase by means of Bacillus aryabhattai metabolism; S3, converting inorganic silicon in the biogas slurry into organosilicon via parallel and tandem reactions under the cascade action of multiple enzymes, which involves the production of unsaturated lipase, oxygenase and carbonic anhydrase by means of Bacillus aryabhattai metabolism; S4, cyclizing straight-chain organosilicon via parallel and tandem reactions under the cascade action of multiple enzymes, which involves the production of amidase, unsaturated lipase, oxygenase and carbonic anhydrase by means of Bacillus aryabhattai metabolism; and S5, performing freeze-drying to obtain the organosilicon hydrogel. Compared with the prior art, the present invention provides a hydrogel with antibacterial, antistatic and corrosion-resistant properties and good biocompatibility. By means of using metabolic enzymes from Bacillus, the process of converting silicate into organosilicon, which originally requires high temperature, is realized by means of biotransformation at ambient temperature. The formed hydrogel can be used for corrosion prevention and antistatic protection of devices such as boilers, and exhibits reversibility; and can also be used for antisepsis and bacteriostasis in cosmetics, and for sand fixation, water retention and promotion of plant growth in agriculture and forestry. Moreover, the hydrogel is recyclable and reusable.

The present invention relates to novel amidase enzymes, their use in the hydrolysis of polyamide oligomers and their use in processes for recycling polyamide polymers. The invention finds particular use in the hydrolysis of oligomers of polyamide 6,6 and therefore in processes for the recycling of this polymer.

The present invention provides deamidase compositions comprising a deamidase inhibitor, wherein the deamidase exhibits less self-deamidation and / or modification to other enzymes after storage.

This invention discloses a nutrient-degrading enzyme, engineered microbial agents, and their application in the nutrient regulation of polymeric slow-release fertilizers, belonging to the field of enzyme engineering technology. This invention utilizes a specific polymeric slow-release fertilizer-degrading enzyme modified by artificial intelligence, which not only retains the degradation activity of the source strain enzyme but also achieves efficient heterologous expression while promoting a dual improvement in stability and catalytic efficiency. The constructed composite engineered strain exhibits no antagonistic effect, enabling low-cost, large-scale production of the degrading enzyme. The composite engineered strain can achieve precise regulation of nutrient release from polymeric slow-release fertilizers. By adjusting the ratio of engineered strains producing peptidases and amidases, precise supply of nutrients to different stages throughout the growth cycle of different crops can be achieved, resulting in complete degradation of polymeric slow-release fertilizers, significantly improving crop yield and nutrient utilization, and showing significant application prospects in the development of precision agriculture.

The invention provides an amidase mutant and application thereof in preparation of (S)-penflufen, and belongs to the technical field of gene engineering and enzyme catalysis. Compared with amidase with an amino acid sequence as shown in SEQ ID No.1, alanine at the 109th site of the amidase mutant mutates into valine and / or leucine at the 232 site of the amidase mutant mutates into arginine. Compared with wild type amidase, the amidase mutant has higher optimum temperature and thermal stability, can catalyze a racemization substrate M-1 (formula I) in a water phase to carry out enzymatic reaction to generate a key chiral intermediate (S)-M-2 (formula II), and further react with a compound M-3 (formula III) to efficiently prepare (S)-penflufen, and the method is mild in reaction condition, simple and convenient to operate and suitable for industrial production. And the method has a good industrial application prospect.

The invention relates to the technical field of enzyme engineering, and discloses a method for preparing a chiral compound through resolution of racemate lactam and application of the method, and the method comprises the following steps: taking racemate lactam as a substrate, and carrying out chiral resolution on the substrate by using amidase or amidase-producing microorganisms to obtain the chiral compound. The method provided by the invention can be specifically applied to chiral resolution of racemate beta-lactam and racemate gamma-lactam, the optical purity of the obtained product reaches 99.0%, and development of a green and environment-friendly biological catalysis chemical process is facilitated; meanwhile, the method provided by the invention can be widely applied to preparation of optical pure chiral drug intermediates of paclitaxel antitumor drugs and nucleoside antiviral drugs.

The invention relates to the technical field of microorganisms, in particular to a fermentation and separation preparation method of N-acetylmuramic acid-L-alanine amidase. According to the present invention, the clostridium beijerinckii CM20 can efficiently produce the N-acetyl muramic acid-L-alanine amidase, and can be adopted as the production strain of the N-acetyl muramic acid-L-alanine amidase, such that the N-acetyl muramic acid-L-alanine amidase can be produced by using the clostridium beijerinckii through fermentation with glucose and other carbohydrates as the carbon source; the production method of the N-acetylmuramic acid-L-alanine amidase, provided by the invention, has the advantages of simplicity, high efficiency, low cost and the like, and an effective method is provided for the microbial fermentation production of the N-acetylmuramic acid-L-alanine amidase.