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5 results about "Nitrilase" patented technology
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Nitrilase enzymes (nitrile aminohydrolase; EC 3.5.5.1) catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, without the formation of "free" amide intermediates. Nitrilases are involved in natural product biosynthesis and post translational modifications in plants, animals, fungi and certain prokaryotes. Nitrilases can also be used as catalysts in preparative organic chemistry. Among others, nitrilases have been used for the resolution of racemic mixtures. Nitrilase should not be confused with nitrile hydratase (nitrile hydro-lyase; EC 4.2.1.84) which hydrolyses nitriles to amides. Nitrile hydratases are almost invariably co-expressed with an amidase, which converts the amide to the carboxylic acid. Consequently, it can sometimes be difficult to distinguish nitrilase activity from nitrile hydratase plus amidase activity.
An enzymatic engineering method and its application in biocatalysis
This invention belongs to the field of enzyme engineering technology, specifically relating to an enzyme engineering method and its application in biocatalysis. Based on the excellent performance optimization capabilities of the deep learning-guided protein sequence design model ProteinMPNN, this invention developed a computational design framework called DeepPCD. This framework integrates deep learning-guided sequence design, physical energy calculation, and molecular dynamics simulation, constructing a three-level intelligent screening process from "massive generation" to "high-precision prediction." Using this strategy, nitrile hydrolase (EC 3.5.5.1) was mutated, resulting in a nitrile hydrolase mutant with improved stability, specificity, and enzyme activity, capable of efficiently and specifically catalyzing the hydrolysis of nitrile compounds to carboxylic acids.
Owner:ZHEJIANG UNIV OF TECH
Nitrilase mutants and their use in the synthesis of gamma-aminobutyric acid derivatives
The application discloses a nitrilase mutant and application thereof in synthesis of gamma-aminobutyric acid derivatives, and relates to a nitrilase mutant with improved catalytic activity and stereoselectivity, which is used for efficiently catalyzing hydrolysis of 2-substituted succinonitrile to synthesize 3-substituted-3-cyanopropionic acid, and further synthesizing gamma-aminobutyric acid derivatives through hydrogenation, thereby laying a foundation for industrialized production of gamma-aminobutyric acid drugs synthesized by the nitrilase method.
Owner:ZHEJIANG UNIV OF TECH
A method for the enzymatic synthesis of 2-(4-nitrophenyl)butyric acid, an intermediate of indobufen.
This invention belongs to the fields of enzyme engineering and biotechnology, and discloses a nitrile hydrolase mutant, its encoding gene, recombinant vector, recombinant genetically engineered bacteria, enzyme preparation, and applications. The nitrile hydrolase mutant is obtained by single-point or multi-point mutation of amino acids 8, 17, 35, or 150 of the nitrile hydrolase amino acid sequence shown in SEQ ID NO. 2. The nitrile hydrolase mutant of this invention exhibits 32-fold increased activity and 15.8-fold increased yield compared to the wild-type Gi-Nit, laying the foundation for the industrial enzymatic synthesis of 2-(4-nitrophenyl)butyric acid.
Owner:PEKING UNIVERSITY THIRD HOSPITAL (THE THIRD CLINICAL MEDICAL SCHOOL OF PEKING UNIVERSITY) +2
Nitrilase mutants and uses thereof
The application provides a nitrilase mutant and its application in synthesis of p-cyanobenzoic acid. The application provides a nitrilase mutant with improved activity in catalytic synthesis of p-cyanobenzoic acid, wherein the mutant protein is a non-natural protein, and one or more substitution mutations exist in the amino acid sequence shown in SEQ ID NO:1, V65, H137, M193, I197, Q201, H208 and R281 are catalysts, and the mutant protein has significantly improved activity in catalyzing p-xylyl cyanide to generate p-cyanobenzoic acid, and the mutant protein is mutated in two or more core amino acids of wild-type nitrilase which are related to enzyme catalytic activity. In a preferred example, the conversion rate is 99% at a p-xylyl cyanide concentration of 600 g / L.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
A process for the enzymatic preparation of (r)-2-trifluoromethyl-2-hydroxypropionic acid
This invention discloses an enzymatic method for preparing (R)-2-trifluoromethyl-2-hydroxypropionic acid. The method uses 3,3,3-trifluoro-2-hydroxy-2-methylpropionitrile as a raw material, which is hydrolyzed by a nitrile hydrolase to obtain (R)-2-trifluoromethyl-2-hydroxypropionic acid. The method disclosed in this invention has a short route, high efficiency, and simple operation, making it more suitable for industrial production.
Owner:SYNCOZYMES SHANGHAI