128 results about "Pseudomonas putida" patented technology

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The invention belongs to the technical field of microorganisms, discloses a strain of pseudomonas putida and application of the strain of pseudomonas putida as fish probiotics, and particularly discloses a strain of pseudomonas putida. The invention provides a symbiotic pseudomonas putida CD separated from fish, and the strain has relatively strong fat utilization capability, can generate a plurality of beneficial extracellular metabolites, can promote metabolism of fish lipids, carbohydrates and proteins and weight gain, and also can improve the iron content and hemoglobin content in fish tissues. The pseudomonas putida strain CD disclosed by the invention has good biological safety, and has a wide development prospect in the aspect of application as fish probiotics.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

The invention provides a transaminase mutant and application of the transaminase mutant in chiral amine synthesis. According to the transaminase derived from Mycobacterium sp. Provided by the invention, in a 1 mL reaction system for catalyzing N-Boc-3-piperidone to generate N-Boc-3-aminopiperidine, the 24-hour conversion rate is 95%, and the e.e. Value of the product is greater than 99%. On the basis, the invention further provides a series of mutants of the transaminase, the relative enzyme activity of the mutants reaches 153%-293%, and the catalytic activity of the transaminase is further improved. Wherein the 34-hour conversion rate of the optimal transaminase mutant is increased to 90% or above under the condition of 120g / L substrate concentration in a 10mL reaction system, and the e.e. Value of the product is greater than 99%. The production technology has the advantages of high catalytic activity, easiness in fermentation, environment friendliness and the like, and has good development and application values.

The invention relates to the technical field of microorganisms, and discloses a pseudomonas putida strain and a preparation method and application thereof.The strain is pseudomonas putida which is separated from soil and has the capacity of dissimilatory reduction of nitrate into ammonium and is classified into a new pseudomonas putida strain based on GTDB-TK. A dissimilatory NOreductive gene cluster, namely an nirBD gene cluster, exists on a genome of the gene, nirK / nirS genes are deleted, and NOs can be reduced into NH under the anaerobic culture condition. According to the application of the pseudomonas putida strain in improvement of soil nitrogen management, the pseudomonas putida strain can be mixed with organic fertilizer for use so as to enhance the effective utilization and conversion capacity of nitrogen in soil. By means of the pseudomonas putida strain, NON can be reduced into NHH under the anaerobic condition, the culture method is simple, the growth speed is high, variation is not prone to occurring, and a brand new microbial resource is provided for soil nitrogen cycle research.

The invention relates to a talaromyces purpureogenus strain D01 and application of the talaromyces purpureogenus strain D01, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D01 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the talaromyces purpurogenus D01 is GDMCC NO.66458. The strain is obtained by separating and purifying soil samples near exits of Guangzhou Taitai and subway stations, and tests show that bacteriostatic active substances generated by fermentation of the strain have good bacteriostatic effects on burkholderia cepacia, staphylococcus aureus, pseudomonas putida, pseudomonas aeruginosa, escherichia coli and candida albicans. And the antibacterial active substance has high temperature resistance and excellent preservative effect, can be used as an antibacterial preservative raw material to be applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention relates to pseudomonas putida engineering bacteria for producing rhamnolipid as well as a construction method and application of the pseudomonas putida engineering bacteria, the engineering bacteria take pseudomonas putida KT2440 with phaC1, phaC2, algD and fliC gene deletion as a chassis cell, and rhlAB, rmlACBD, algC and FabY genes are over-expressed in the chassis cell. Pseudomonas putida KT2440 with biological safety is used as a chassis cell for gene modification, the constructed engineering bacteria are used for fermentation production of rhamnolipid, on the basis of rhamnolipid yield increase, good physiological adaptability and fermentation stability are shown, and the rhamnolipid yield is increased. And the modified engineering strain can stably and efficiently produce rhamnolipid with a specific structure.

The invention provides a microbial composite remediation liquid organic fertilizer and a preparation method thereof, and aims to solve the problems of single function, limited pollution abatement capability and the like of a traditional liquid fertilizer. Through synergism of strains of bacillus velezensis, bacillus methylotrophicus, pseudomonas putida and methanogenic archaea, recycling of agricultural wastes such as straw and mushroom dreg compost and decomposition, extraction of humic acid through enzymolysis and a formula of green additives (mineral source potassium fulvate, sophorolipid and the like), chemical passivation of heavy metals, pesticide degradation and soil improvement are realized; and dynamic fermentation control and membrane separation processes are combined, so that the method has the characteristics of efficient repair and low-carbon sustainability, and an integrated solution is provided for pollution treatment of complex farmlands.

The invention relates to a lactobacillus plantarum LAS3 strain and an application of the lactobacillus plantarum LAS3 strain, and belongs to the technical field of microorganisms. The lactobacillus plantarum LAS3 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the lactobacillus plantarum LAS3 is GDMCC NO.66440. The strain is separated, purified and screened from Guizhou sour fish, and experiments show that the strain has excellent pH tolerance and sodium chloride tolerance; fermentation liquor of the strain has a good antibacterial effect on multiple common pathogenic bacteria such as pseudomonas aeruginosa, burkholderia cepacia, pseudomonas putida, staphylococcus aureus, bacillus subtilis and candida albicans, and bacteriocin generated by the strain has excellent antibacterial and preservative effects and also has excellent thermal stability and pH stability. The antibacterial preservative can be used as an antibacterial preservative raw material, is applied to food, health care products or daily chemical products, and has wide application prospects and important conversion research values.

The invention discloses a recombinant strain for efficiently expressing xanthine oxidase as well as preparation and application of the recombinant strain, and belongs to the technical field of enzyme engineering. According to the recombinant strain, four kinds of recombinant Pseudomonas putida strains are designed, and the strains can be used for efficiently expressing xanthine oxidase. The recombinant strain has the advantages of high expression quantity, simple affinity purification process flow, high purification yield and low production cost, and the recombinant xanthine oxidase has the specific activity similar to that of xanthine oxidase sold in the market at present and is suitable for various applications. The recombinant strain can realize high-efficiency expression of xanthine oxidase, and high-efficiency expression of xanthine oxidase is realized in P.putida through a gene recombination technology; the expressions are induced by adding an inducer, so that the maximum expression quantity of the xanthine oxidase is realized.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention relates to the technical field of microorganisms, in particular to growth-promoting pantoea TY-88 and application thereof in promotion of waxy corn seed germination and seedling growth, the preservation unit of the growth-promoting pantoea TY-88 is China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025945, and the preservation date is April 30, 2025. The growth-promoting pantoea TY-88 provided by the invention has better capabilities in the aspects of phosphate solubilization, nitrogen fixation, potassium solubilization and siderophore production; when the biological agent prepared from the growth-promoting pantoea is used for seed soaking treatment, waxy corn seed germination, seedling growth and development and substance accumulation of the overground part and the underground part can be remarkably promoted, and economic benefits are improved on the premise that the environment is not polluted.

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The invention discloses a ginger endophytic bacterium CQG8 for promoting plant growth and a preparation method and application thereof, the ginger endophytic bacterium CQG8 is classified and named as Pseudomonas putida, is preserved in Guangdong Microbial Culture Collection Center, the address is 59 building, No.100 courtyard, Xianlie Middle Road, Guangzhou City, the preservation date is May 14, 2025, and the preservation number is GDMCC NO: 66334. The ginger endophytic bacterial strain has the activity of producing indoleacetic acid, dissolving phosphorus, dissolving potassium, fixing nitrogen and producing siderophores, and can be used for promoting the growth of ginger and the germination of corn seeds.

The invention discloses a composite biocontrol bacterium for preventing and treating tobacco bacterial wilt and application of the composite biocontrol bacterium. Belongs to the technical field of biological control. The compound biocontrol bacteria comprise pseudomonas putida YZ03102604, bacillus safensis YZ03102605 and bacillus velezensis YZ23120529, and the compound biocontrol bacteria comprise pseudomonas putida YZ03102604, bacillus safensis YZ03102605 and bacillus velezensis YZ23120529. The composite biocontrol bacterium can be prepared into a microbial inoculum with auxiliary materials, and the microbial inoculum is applied during tobacco transplanting and after tobacco transplanting. Potting and field experiment results show that the composite biocontrol bacterium can significantly reduce the morbidity and disease index of bacterial wilt, the prevention and treatment effect reaches 76% or above, and the composite biocontrol bacterium is superior to a single strain and other combinations and has a good biocontrol application prospect.

The present invention relates to the field involved in the production of phenylbutanone or phenylbutanone derivative compounds, such as frambinone or zingerone, and in particular strains genetically modified to express a benzalacetone reductase.

A Pseudomonas putida ω-aminotransferase gene with broad substrate spectrum and an application thereof are provided. According to the disclosure, codon optimization is carried out on an ω-transaminase gene, and the nucleotide sequence of the ω-transaminase gene is shown in SEQ ID NO: 1. The crude enzyme solution, crude enzyme powder and pure enzyme of ω-aminotransferase prepared from ω-aminotransferase gene can be used as an enzyme preparation to catalyze the transamination reaction of substrates to prepare chiral amino alcohols and chiral amines. The substrate spectrum of the recombinant ω-transaminase obtained by the disclosure is broad, and 15 kinds of chiral amino alcohols and chiral amines with high added value are asymmetrically catalyzed and synthesized; in particular, 4-hydroxy-2-butanone is catalyzed to produce pharmaceutical intermediate (R)-3-amino-1-butanol.

This invention relates to the field of synthetic biology, and more particularly to a microbial cell factory for producing catechols. The invention uses *Pseudomonas putida* strains with the pcaHG, catA, and catA2 genes knocked out as substrate bacteria, and introduces exogenous AroY and / or kpdB genes, or increases the expression of endogenous genes VanAB and PobA. Through a strategy of cofactor regulation and key enzyme overexpression, lignin-derived monomers are efficiently converted into catechols, achieving a conversion rate of 98.5% and a maximum yield of 14.1 mM, a significant improvement compared to the previous conversion rate of 64%.

This invention belongs to the field of bio-mineral fertilizer technology, specifically relating to a microbial liquid compound agent and bio-mineral fertilizer that combine coal gangue activation and heavy metal remediation. The microbial liquid compound agent comprises the following functional strains prepared through three-stage sequential inoculation and fermentation using electrochemical low-Hertz water: *Pseudomonas putida* (a remedial bacterium), *Azotobacter chrysogenum* (a nitrogen-fixing bacterium), *Azospirillum brasiliensis* (a growth-promoting bacterium), *Pseudomonas fluorescens* (a phosphate-solubilizing bacterium), *Bacillus subtilis* (a biocontrol bacterium), *Bacillus mucilaginosus* (a potassium-solubilizing bacterium), *Bacillus megaterium* (a silica-solubilizing bacterium), and *Trichoderma echinococcus* (a synergistic bacterium). The bio-mineral fertilizer is prepared using the aforementioned microbial liquid compound agent, with coal gangue powder as the main raw material. The scientific combination of different strains provided by this invention achieves efficient improvement of heavy metal-contaminated land and increased crop yields. Simultaneously, it transforms coal gangue into a valuable resource, solving the pollution problem caused by the large-scale accumulation of coal gangue, and possesses significant economic and social value.

The invention discloses a nitrilase mutant and an application technology thereof in synthesis of nicotinic acid, and belongs to the technical fields of gene engineering, enzyme engineering and biological catalysis. The nitrilase mutant disclosed by the invention is obtained by mutating nitrilase from a source of Pseudomonas putida CGMCC (China General Microbiological Culture Collection Center) 3830, and the heterologous expression of the nitrilase mutant in corynebacterium glutamicum is successfully realized. The optimal mutant can efficiently catalyze a substrate 3-cyanopyridine to synthesize 550.68 g / L of nicotinic acid, and the conversion rate is 91.37%.

This invention relates to a polymyxin Bacillus strain ( Paenibacillus polymyxa This invention relates to B01 and its applications, belonging to the field of microbial technology. The *Bacillus polymyxa* B01 strain disclosed in this invention has been deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC NO.66594. This strain was isolated, purified, and screened from soil samples. Experiments show that this strain has excellent pH and high-temperature tolerance, and the antibacterial active substances produced by its fermentation have good antibacterial effects against *Escherichia coli*, *Pseudomonas aeruginosa*, *Pseudomonas putida*, *Staphylococcus aureus*, *Candida albicans*, *Aspergillus brasiliensis*, *Propionibacterium acnes*, and *Malassezia*. Furthermore, the antibacterial active substances exhibit excellent heat resistance, cold resistance, light stability, pH stability, and preservative effects. It is a mild and low-irritant raw material that can be used to prepare daily chemical products and pharmaceuticals, possessing broad application prospects and significant value for transformational research.

The invention relates to a rapid detection method of pseudomonas putida and application thereof, and the detection method comprises the following steps: (1) taking a sample to be detected, carrying out enrichment culture in an enrichment liquid, centrifugally collecting thalli subjected to enrichment culture, and carrying out DNA extraction on the thalli; (2) carrying out PCR (Polymerase Chain Reaction) amplification on the extracted DNA, and determining whether the sample to be detected contains pseudomonas putida or not according to an amplification result; a target gene subjected to PCR amplification is an rpoD gene. Specific detection is achieved through the targeted rpoD gene, and the detection period is shortened to be within 24 h in combination with optimized DNA extraction and a qPCR system. In addition, the whole-process quality control design can effectively avoid false positive / false negative results, 96 samples can be processed in a single batch, and an efficient and reliable quality control scheme is provided for the cosmetic industry.

The present application belongs to the field of microbial genetic engineering technology, and particularly relates to a recombinant bacterium for degrading crab shells, a construction method and application thereof. The present application carries out metabolic engineering modification on Pseudomonas putida KT2440 capable of utilizing crab shell substrate to grow and carry out life activities, utilizes overexpression of exogenous protease genes lasBT and IV to construct overexpression strains KT +IV , KT +lasBT The two modified engineering bacteria improve the protein utilization rate, enable the strains to better adapt to the crab shell substrate fermentation environment, enhance the growth ability of the strains, promote the high-value utilization of crab shells, and lay a foundation for realizing efficient and large-scale conversion of crab shells into high-value chemicals.

Inventors describe herein the functional and structural characteristics of the MGL from the anaerobic alkaliphilic thermophile Thermobrachium celere (TcMGL), which was compared to that from Pseudomonas putida (PpMGL). Catalysis was limited to L-Met, L-homocysteine, and L-cysteine, with the highest catalytic efficiency (keat / KM) towards L-Met. The half-life of active TcMGL in human plasma at 37°C was 9.2 hour. Half-life increased from 11.3 hour to 66.7 hour with increasing PLP concentration from 2 to 200 µM, in much greater magnitude than in PpMGL case. TcMGL crystal structure resolution revealed that the enzyme is a tetramer of two catalytic dimers containing a mobile region with open and closed conformations resulting in differences for access to the active site. TcMGL possesses favourable characteristics which makes it a suitable candidate for treatment of cancer. The present invention relates to a polypeptide comprising an amino acid sequence as set forth by SEQ ID NO: 1 or a function-conservative variant thereof.