14 results about "Pseudomonas putida" patented technology

The present application relates to the technical field of biology, and discloses a construction method of a Pseudomonas putida suicide vector and application thereof.The construction method of the Pseudomonas putida suicide vector provided by the present application takes a pK18mobsacB vector as a template to obtain a vector skeleton by amplification, and then takes Pseudomonas putida KT2440 bacterial liquid as a template to obtain a strong promoter Prib, upper and lower homologous arms of a gene x to be knocked out, and connects the upper and lower homologous arms to the vector skeleton to obtain the Pseudomonas putida suicide vector.The strong promoter Prib can increase the expression amount of sacB sucrose in the Pseudomonas putida, and enhance the sucrose lethal effect.The Pseudomonas putida suicide vector obtained can be introduced into the Pseudomonas putida, and then the strain with the target gene to be knocked out deleted can be obtained efficiently and accurately through kanamycin positive screening and sucrose negative screening.

The application discloses a kind of recombination of Pseudomonas putida for producing L-lactic acid using whole-component lignocellulose hydrolysate and its construction method and application, belong to the field of metabolic engineering.The application uses Pseudomonas putida KT2440 as chassis, realizes carbon flow to pyruvic acid convergence and prevents product degradation by knocking out aceEF 、 ppsA And lldD Gene;By in-situ replacement, introduce optimized Lactobacillus plantarum ldhL1 Reconfiguration L-lactic acid synthesis pathway;By integration xylD-yjhH Tandem expression cassette enables xylose utilization;And by quorum sensing promoter P Rox306 Overexpression maeB Realize funnel conversion of lignin monomer.The application innovatively uses lignin derivatives in hydrolysate as natural donor of endogenous acetyl-coenzyme A, makes up lethal growth defect caused by knocking out aceEF The engineering bacteria of the application realize the synchronous high-efficiency conversion of glucose, xylose and aromatic monomer under the condition of not adding exogenous auxiliary carbon source and chemical inducer, which greatly reduces the industrial fermentation cost.

This invention belongs to the field of biotechnology, specifically relating to a *Pseudomonas putida* genome insertion site library and its construction method. This invention, for the first time, systematically screened and obtained 17 specific genome insertion sites suitable for *Pseudomonas putida*. The constructed insertion site library covers different expression intensities, filling the gap in existing technologies lacking efficient integration sites for *Pseudomonas putida*, and providing diverse options for personalized expression of target genes. The screened insertion sites are all located in the regions of opposing transcription genes, avoiding interference with the host's basal metabolic network. The recombinant strain exhibits stable growth performance without significant growth inhibition, making it suitable for large-scale industrial applications. The integration method, based on homologous recombination, achieves stable integration of the target gene without plasmid maintenance, avoiding expression instability caused by plasmid loss and copy number fluctuations. It also reduces the continuous use of antibiotics, lowering industrialization costs and environmental risks, and the construction method is simple and efficient.

This invention relates to a bacterium that degrades chlorobenzene pollutants and its applications. The bacterium is a *Pseudomonas putida* strain, named *Pseudomonas putida* BS-1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 2024441 on March 11, 2024. Compared with existing technologies, the strain provided by this invention can completely degrade a complex pollutant mixture of dichlorobenzene, chlorobenzene, and benzene within 48 hours. It can survive for a long time and maintain its degradation activity under actual pollution conditions of complex pollution and low temperatures. Furthermore, the functional genes related to the degradation of chlorobenzene pollutants are located on a plasmid, which helps the indigenous bacterium acquire the relevant functions for degrading chlorobenzene pollutants. This strain can be applied to in-situ bioremediation of groundwater with complex chlorobenzene pollution in chemical industrial parks, effectively overcoming the technical shortcomings of traditional reduction dechlorination remediation technologies, which often result in incomplete reduction of products, and achieving green, sustainable, and thorough purification without secondary pollutants.

The application discloses a plant salt-tolerance promoting active metabolite separated from a rhizosphere growth-promoting Pseudomonas proteus and application thereof. The metabolite is dibutyl phthalate (DBP) with a molecular formula of C 16 H 22 O4, has the effect of improving the salt tolerance of rice, and obviously increases the plant height, root length, aboveground fresh weight and underground fresh weight of the rice, is stable in effect, and has a good application prospect; and can be used as a preparation for improving the salt tolerance of crops in agricultural green production.

The application provides a composite microbial liquid for saline soil improvement and crop growth promotion and application thereof, and the composite microbial liquid comprises Bacillus amyloliquefaciens S1-13 liquid, Bacillus thuringiensis SP1-14 liquid, Pseudomonas putida SE1-6 liquid and Bacillus pacificus RC3-7 liquid. Each strain in the composite microbial liquid has specific functions, and after reasonable compounding, the composite microbial liquid can significantly reduce the pH of the soil, improve the soil microbial activity and increase the crop yield.

The present invention relates to the production of aminobenzoic acid using a process having the following steps: (A) providing a fermentation broth containing aminobenzoate anions by carrying out a fermentation process in the presence of a microorganism selected from the group consisting of Escherichia coli, Pseudomonas putida, Corynebacterium glutamicum, Bacillus coagulans or a mixture of two or more of the aforementioned microorganisms, and subsequently carrying out a clarification process, (B) setting the pH value of the fermentation broth containing aminobenzoate anions to 3.0 to 5.5 by adding formic acid, (C) isolating the aminobenzoic acid precipitated in step (B), and (D) introducing the mother liquor precipitated in step (C) partially or entirely into the fermentation and depleting the formate anions and formic acid contained in the mother liquor by (i) an added enzyme preparation and / or (ii) the microorganism used in step (A) and / or (iii) an additional microorganism different from the microorganism used in step (A). The aminobenzoic acid obtained by this process can be converted into products, in particular anilines, and in the case of anthranilic acid into indigo acid anhydride or anthranilate esters, which can be used as raw materials for the production of polymers such as polyurethanes or poly(anthranilamide).

This invention discloses an aromatic compound transporter protein mutant and its applications, belonging to the field of bioengineering technology. The mutant is based on *Pseudomonas putida* (…). Pseudomonas putida The outer membrane transport protein derived from [a specific source] is obtained by site-directed substitution of key residues in its gating loop. Specifically, this includes replacing phenylalanine at position 120 with glycine, leucine at position 121 with glycine, and glutamine at position 122 with glycine, or one or more combinations thereof. Preferred mutants include single mutant F120G, double mutant F120G / L121G, or triple mutant F120G / L121G / Q122G, and conserved substitution derivatization can also be performed based on these mutants. These mutants can be used for the efficient microbial production and cell tolerance regulation of aromatic compounds, based on the physicochemical properties of the target product, and have significant application value in green biomanufacturing, fermentation engineering, and bioremediation.

This invention relates to a recombinant photosynthetic bacterial strain with high tocopherol production, its construction method, and its applications. The recombinant strain knocks out the photosynthetic gene repressor transcription factor PpsR and integrates the mevalonate MEV pathway gene from *Paragonimococcus zeatans*, the p-hydroxyphenylpyruvate dioxygenase (HPPD) gene from *Pseudomonas putida*, the urokinase chlorophyllase (HPT) gene from *Synostemma pentaphyllum*, the tocopherol cyclase (TC) gene from *Arabidopsis thaliana*, and feedback-resistant 3-deoxy-D-arabinohepenoyl-7-phosphate synthase (AroG*). D146N Genes and prephenyl acid dehydrogenase TyrA* M53I Genes. This recombinant photosynthetic bacterium strain achieves highly efficient heterologous synthesis of tocopherol by knocking out regulatory genes and integrating multiple metabolic modules, with a fermentation yield of up to 3.51 g / L, showing broad prospects for industrial application.

The application provides a composite microbial growth-promoting and disease-resistant bactericide, a preparation method and application thereof. The composite microbial growth-promoting and disease-resistant bactericide is prepared by mixing B24 bactericide of Pseudomonas putida and 79-1 bactericide of Bacillus subtilis. Since there is no obvious antagonism between the two, the growth-promoting effect of the B24 bactericide of Pseudomonas putida can be utilized, and the antagonism of the 79-1 bactericide of Bacillus subtilis to J-9, B152 and 617-3 of Fusarium during the growth of crops can be utilized, so that the rapid growth of crops can be effectively promoted.

The application discloses a kind of pseudomonas putida recombinant bacteria and construction method and application, construction method steps are as follows: (1) knocking out pseudomonas putida KT2440 PP_3425 Gene and PP_3283 Gene;(2) overexpression vanAB Gene, the present application takes lignin derivative as main carbon source, and raw material cost is significantly reduced, and source green renewable;Recombinant bacteria performance is excellent, and PHA production is significantly improved: the recombinant bacteria constructed in the application not only retains the efficient PHA synthesis capacity of wild bacteria KT2440 endogenous, natural stress resistance and growth robustness, but also significantly improves the growth stability and PHA synthesis efficiency under high concentration lignin derivative.The experiment proves: the PHA content of recombinant bacteria is increased by about 46.4% compared with wild bacteria, and the PHA concentration is increased by about 78.3%, which indicates that it is greatly improved in intracellular synthesis capacity and fermentation total yield.

This invention relates to a biomodified anode material, comprising: (A) *Pseudomonas putida* YT-1; (B) *Shewanella oneidensis* MR-1; (C) pyrite; (D) sodium alginate-PVA bilayer hydrogel; and (E) a substrate. The above-mentioned anode material of this invention can be used to fabricate microbial fuel cells, wherein the anode material regulates the soil "Fe(III)-Fe(II)" cycle through functional microorganisms, thereby affecting the arsenic speciation in soil pore water, inhibiting the anaerobic reduction reaction of pentavalent arsenic at the anode, promoting the migration of soluble trivalent arsenic in pore water to the cathode and its oxidation to pentavalent arsenic, inhibiting the anaerobic reduction reaction of arsenic in the soil, and promoting the co-precipitation of iron and arsenic to reduce the environmental risk of arsenic pollution.

This invention belongs to the field of fertilizer and pesticide technology, specifically relating to a rice-specific fertilizer and pesticide granule. The rice-specific fertilizer and pesticide granule includes a compound microbial agent, which is a mixture of *Pseudomonas putida* and *Bacillus subtilis* inoculants. The *Pseudomonas putida* has the preservation number CCTCC NO: M2020341, and the *Bacillus subtilis* has the preservation number CCTCC HB 20081305. This invention combines a nutrient-supplying fertilizer substrate with a biological control microbial agent, achieving integrated fertilizer and pesticide application. Farmers do not need to apply fertilizer and pesticide separately, which helps reduce labor intensity and planting costs, making it suitable for large-scale, mechanized rice cultivation. Furthermore, the combination of *Pseudomonas putida* and *Bacillus subtilis* provides a better bactericidal effect than a single strain, eliminating the need for additional chemical bactericides. This not only improves disease control but also helps improve the soil micro-ecological environment, achieving ecological planting.

The application discloses an oral composition, a mouth product, a drink and related applications of the oral composition. The oral composition provided by the application comprises a nicotine dehydrogenase, probiotics and food-grade synergistic fiber, the nicotine dehydrogenase is derived from Pseudomonas putida, and the probiotics are Lactobacillus plantarum. The oral composition provided by the application has a nicotine degradation rate of 90.5% in a simulation system. Meanwhile, the composition has excellent processability, gastrointestinal stability, high food compatibility and bioavailability.