204 results about "Pseudomonas putida" patented technology

The invention relates to a salt-tolerant growth-promoting pseudomonas putida PLW1 and application thereof, and belongs to the technical field of microorganisms, the pseudomonas putida PLW1 is preserved in China Center for Type Culture Collection on June 24, 2024, and the preservation number is CCTCC NO: M 20241352; the strain has the functions of degrading organic phosphorus, degrading inorganic phosphorus, producing lipase, producing siderophores and producing indoleacetic acid, and also has good salt-resistant and alkali-resistant characteristics, and the microbial inoculum prepared from PLW1 can promote germination of corn seeds and growth of corn seedlings under salt stress, and has a very wide application prospect.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention discloses application of synbiotics to prevention and control of plutella xylostella pests. Probiotics in the synbiotics are a pseudomonas strain PPF1, the pseudomonas strain PPF1 is classified and named as Pseudomonas putida, and the prebiotic composition is ornithine, alanine, valine, serine, leucine and cycloleucine. The invention further discloses a preparation method of the prebiotic composition. Wherein the pseudomonas can improve the resistance of Chinese cabbage to plutella xylostella. According to the prebiotic composition, the resistance of Chinese cabbage to plutella xylostella is improved, meanwhile, the growth of the pseudomonas strain PPF1 is remarkably promoted, and the colonization ability of the pseudomonas strain PPF1 is improved. The synbiotics are applied into soil by a root irrigation method, so that the feeding area of the cabbage by the plutella xylostella can be remarkably reduced, and the pest of the plutella xylostella in the cabbage can be effectively prevented and controlled.

The invention discloses a microbial agent for improving the yield and quality of strawberries and a preparation method and application thereof, and belongs to the technical field of microorganisms. The microbial agent comprises the following raw materials in parts by weight: 40-50 parts of a compound microbial agent, 0.3-0.5 part of xanthan gum, 1.5-2 parts of glycerol, 4-5 parts of a citric acid-sodium citrate buffer solution, 1-1.5 parts of potassium humate, 0.3-0.5 part of monopotassium phosphate, 0.2-0.3 part of chelated calcium magnesium and the balance of water, wherein the total parts by weight of the raw materials are 100; the compound microbial agent is prepared from arthrobacter Xinjiang, linear Prussella bursa-pastoris and bacillus coagulans. The microbial agent disclosed by the invention can be used for effectively preventing and treating strawberry diseases, including root rot, fusarium wilt, gray mold and anthracnose; through the synergistic effects of hormone secretion, nutrient activation, disease inhibition and the like, the microbial agent comprehensively promotes root development and nutrient absorption of strawberry plants, promotes strawberry growth, remarkably improves the strawberry yield and fruit quality, and meanwhile remarkably reduces the strawberry storage rotting rate.

The invention relates to the technical field of microbial metabolism engineering, in particular to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. The invention relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine, which is characterized in that a transport protein encoding gene rhtA is knocked out in a chassis strain Escherichia coli W3110, a homoserine O-acetyltransferase encoding gene metXcm from marine cyclobacter is over-expressed, an acetyl coenzyme A synthetase encoding gene acs is over-expressed, pantothenic acid kinase coXpp from pseudomonas putida is over-expressed, and the recombinant escherichia coli can be used for efficiently producing O-acetyl-L-homoserine. And knocking out the malate synthetase coding gene aceB to obtain the recombinant escherichia coli for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention does not carry plasmids, does not need to additionally add antibiotics and inducers, and is lower in production cost and more stable. And the yield of the O-acetyl-L-homoserine of the plasmid-free strain is increased to 16.85 g / L from 0 g / L. Therefore, the recombinant escherichia coli provided by the invention has important industrial application value.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention discloses a bacterial strain and a method for producing pyrroloquinoline quinone. The strain is pseudomonas putida N1-1 which is preserved in the China General Microbiological Culture Collection Center on January 2, 2025, the preservation number is CGMCC NO.33266, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing. The strain is separated from myxomycete. The bacterial strain is inoculated into a fermentation culture medium, standing fermentation culture is carried out for 7 days at room temperature under the dark condition, a fermentation product is obtained and filtered, fermentation filtrate containing pyrroloquinoline quinine is obtained, and the yield of pyrroloquinoline quinine reaches 12.21 mg / L.

The invention belongs to the technical field of biological mineral fertilizers, and particularly relates to a microbial liquid complex microbial inoculant with coal gangue activation and heavy metal treatment functions and a biological mineral fertilizer. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation in three stages: repair bacteria pseudomonas putida, nitrogen-fixing bacteria azotobacter chroococcum, growth-promoting bacteria azotobacter braziliensis, phosphate solubilizing bacteria pseudomonas fluorescens, biocontrol bacteria bacillus subtilis and potassium bacteria bacillus mucilaginosus. The silicolytic bacteria are bacillus megatherium; and the synergistic bacteria are trichoderma asperellum. The biological mineral fertilizer is prepared by using the microbial liquid complex microbial inoculant and using coal gangue powder as a main raw material. The scientific compatibility of different strains provided by the invention realizes the efficient improvement of the heavy metal polluted land and the yield increase of crops, changes the coal gangue into valuables, solves the problem of pollution caused by the accumulation of a large amount of coal gangue, and has important economic and social values.

The present invention provides a method for treating heap leaching cyanide tailings of gold mines, a composite bacterial community, a bacterial agent and a preparation method. The composite bacterial community comprises a TDB-1 strain, a TCD-1 strain and a Z-90 strain, wherein the TDB-1 strain is a Pseudomonas putida strain, and the deposit number is CGMCC No.21602. The TCD-1 strain is a Methylorubrum pseudosasae, and the deposit number is CGMCC NO.24728. The Z-90 strain is a Burkholderia sp., and the deposit number is CGMCC No.9970. The above-mentioned composite bacterial community can effectively remove cyanide, thiocyanide and metal-cyanide complexes. The present method has the advantages of simple operation, high reduction efficiency, stable effect, good impact resistance and low cost.

The invention belongs to the technical field of microorganisms, discloses a strain of pseudomonas putida and application of the strain of pseudomonas putida as fish probiotics, and particularly discloses a strain of pseudomonas putida. The invention provides a symbiotic pseudomonas putida CD separated from fish, and the strain has relatively strong fat utilization capability, can generate a plurality of beneficial extracellular metabolites, can promote metabolism of fish lipids, carbohydrates and proteins and weight gain, and also can improve the iron content and hemoglobin content in fish tissues. The pseudomonas putida strain CD disclosed by the invention has good biological safety, and has a wide development prospect in the aspect of application as fish probiotics.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

Provided are methods, prokaryotic host cells, expression vectors, and kits for the production of psilocybin derivatives produced using at least one psilocybin production gene. The methods enable production of psilocybin derivatives and related compounds including non-naturally occurring species. In certain embodiments, a prokaryotic host cell is selected from the group consisting of from the group consisting of Escherichia coli, Corynebacterium glutamicum, Vibrio natriegens, Bacillus subtilis, Bacillus megaterium, Escherichia coli Nissle 1917, Clostridium acetobutlyicum, Streptomyces coelicolor, Lactococcus lactis, Pseudomonas putida, Streptomyces clavuligerus, and Streptomyces venezuelae.

The invention discloses Pseudomonas putida, a bacterial agent thereof and an application of the Pseudomonas putida in chemical wastewater treatment, the Pseudomonas putida is named as PH-20 and preserved in the China General Microbiological Culture Collection Center on March 17, 2025, and the preservation number of the strain is CGMCC No. 33831. The invention further discloses a preparation method of the Pseudomonas putida. The pseudomonas putida disclosed by the invention can be used for rapidly degrading polybenzoic acid such as terephthalic acid, phthalic acid, isophthalic acid, trimellitic acid, trimellitic acid and pyromellitic acid in wastewater under the conditions of high salt and low temperature, and the degradation effect is still excellent under the condition that various pollutants coexist.

The invention provides a transaminase mutant and application of the transaminase mutant in chiral amine synthesis. According to the transaminase derived from Mycobacterium sp. Provided by the invention, in a 1 mL reaction system for catalyzing N-Boc-3-piperidone to generate N-Boc-3-aminopiperidine, the 24-hour conversion rate is 95%, and the e.e. Value of the product is greater than 99%. On the basis, the invention further provides a series of mutants of the transaminase, the relative enzyme activity of the mutants reaches 153%-293%, and the catalytic activity of the transaminase is further improved. Wherein the 34-hour conversion rate of the optimal transaminase mutant is increased to 90% or above under the condition of 120g / L substrate concentration in a 10mL reaction system, and the e.e. Value of the product is greater than 99%. The production technology has the advantages of high catalytic activity, easiness in fermentation, environment friendliness and the like, and has good development and application values.

The invention discloses a method for carrying out various machine learning modeling by utilizing global public data to construct a synthetic flora with broad-spectrum adaptability, and the constructed and simplified synthetic flora is composed of three strains of bacteria, namely, Pseudomonas putida YDTA3, Pseudomonas putida PPF1 and Snotrophomonas sp. Syn-C. The invention also discloses a method for constructing the synthetic flora with broad-spectrum adaptability, and the constructed and simplified synthetic flora is composed of three strains of bacteria, namely Pseudomonas putida YDTA3, Pseudomonas putida PPF1 and Snotrophomonas sp. Syn-C. Compared with a single bacterium, the synthetic flora constructed by the invention has higher environmental adaptability, and the strain for constructing the synthetic flora is irreplaceable and unique. After the synthetic flora is added to the rhizosphere of the continuous cropping tomato, the pathogenicity of pathogenic bacteria is inhibited, so that the morbidity of the bacterial wilt of the continuous cropping tomato is remarkably reduced.

The invention relates to the technical field of microorganisms, and discloses a pseudomonas putida strain and a preparation method and application thereof.The strain is pseudomonas putida which is separated from soil and has the capacity of dissimilatory reduction of nitrate into ammonium and is classified into a new pseudomonas putida strain based on GTDB-TK. A dissimilatory NOreductive gene cluster, namely an nirBD gene cluster, exists on a genome of the gene, nirK / nirS genes are deleted, and NOs can be reduced into NH under the anaerobic culture condition. According to the application of the pseudomonas putida strain in improvement of soil nitrogen management, the pseudomonas putida strain can be mixed with organic fertilizer for use so as to enhance the effective utilization and conversion capacity of nitrogen in soil. By means of the pseudomonas putida strain, NON can be reduced into NHH under the anaerobic condition, the culture method is simple, the growth speed is high, variation is not prone to occurring, and a brand new microbial resource is provided for soil nitrogen cycle research.

The invention relates to a talaromyces purpureogenus strain D01 and application of the talaromyces purpureogenus strain D01, and belongs to the technical field of microorganisms. The talaromyces purpurogenus D01 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the talaromyces purpurogenus D01 is GDMCC NO.66458. The strain is obtained by separating and purifying soil samples near exits of Guangzhou Taitai and subway stations, and tests show that bacteriostatic active substances generated by fermentation of the strain have good bacteriostatic effects on burkholderia cepacia, staphylococcus aureus, pseudomonas putida, pseudomonas aeruginosa, escherichia coli and candida albicans. And the antibacterial active substance has high temperature resistance and excellent preservative effect, can be used as an antibacterial preservative raw material to be applied to the fields of food, health care products or daily chemical products and the like, and has wide application prospects and important conversion research values.

The invention discloses an explosive molecule detection sensing element applicable to a high-temperature condition and application of the explosive molecule detection sensing element. The promoter element is derived from a genome of pseudomonas putida, a nucleotide sequence shown in SEQ ID NO.1 is obtained through transcriptomics analysis and screening and escherichia coli high-temperature screening, and the promoter element has the obvious explosive molecule detection capacity under the high-temperature condition. In addition, the method has the advantage of adapting to high-temperature, green and efficient detection.

The invention discloses a combined preparation for improving rice quality and application thereof, and belongs to the technical field of crop planting. The combined preparation is prepared from the following raw materials in parts by weight: radix angelicae sinensis, radix paeoniae alba, rhizoma atractylodis macrocephalae, fructus forsythiae, oyster mushroom bran, needle mushroom bran, a microbial agent, ammonium nitrate, potassium nitrate, ammonium molybdate, DTA-6, zinc sulfate and monopotassium phosphate. The microbial agent is prepared from bacillus megatherium, pseudomonas putida, serratia marcescens and saccharomycetes. The combined preparation for improving the rice quality is prepared by taking natural Chinese herbal medicines, fungus chaff, microbial strains and a plant growth regulator as raw materials. The combined preparation is rich in polysaccharide, amino acid, chitosan oligosaccharide and other components and active microbial agents, and can promote the growth and development of rice and improve the quality of rice. The combined preparation provided by the invention provides a new technical thought for rice quality improvement.

The invention relates to pseudomonas putida engineering bacteria for producing rhamnolipid as well as a construction method and application of the pseudomonas putida engineering bacteria, the engineering bacteria take pseudomonas putida KT2440 with phaC1, phaC2, algD and fliC gene deletion as a chassis cell, and rhlAB, rmlACBD, algC and FabY genes are over-expressed in the chassis cell. Pseudomonas putida KT2440 with biological safety is used as a chassis cell for gene modification, the constructed engineering bacteria are used for fermentation production of rhamnolipid, on the basis of rhamnolipid yield increase, good physiological adaptability and fermentation stability are shown, and the rhamnolipid yield is increased. And the modified engineering strain can stably and efficiently produce rhamnolipid with a specific structure.

The invention provides a microbial composite remediation liquid organic fertilizer and a preparation method thereof, and aims to solve the problems of single function, limited pollution abatement capability and the like of a traditional liquid fertilizer. Through synergism of strains of bacillus velezensis, bacillus methylotrophicus, pseudomonas putida and methanogenic archaea, recycling of agricultural wastes such as straw and mushroom dreg compost and decomposition, extraction of humic acid through enzymolysis and a formula of green additives (mineral source potassium fulvate, sophorolipid and the like), chemical passivation of heavy metals, pesticide degradation and soil improvement are realized; and dynamic fermentation control and membrane separation processes are combined, so that the method has the characteristics of efficient repair and low-carbon sustainability, and an integrated solution is provided for pollution treatment of complex farmlands.

The invention discloses a microbial remediation method for improving desert steppe soil quality, which comprises the following steps: sowing grass seeds and applying microbial fertilizer to a pretreated land parcel, sowing 1-5kg of grass seeds per mu, applying 15-45kg of fertilizer per mu, sowing line spacing of 35cm and sowing depth of 2-3cm, and the grass seeds comprise 40-60 parts by weight of Aconitum carmichaeli, 5-15 parts by weight of Astragalus adsurgens and 30-50 parts by weight of Mongolian wheatgrass; the microbial fertilizer comprises nitrogen-fixing bacteria, phosphate solubilizing bacteria and growth-promoting bacteria, the content of organic matters is greater than or equal to 40%, and the pH value is 6.5-7.5; wherein nitrogen-fixing bacteria in the microbial fertilizer are alfalfa rhizobium or soybean rhizobium; the phosphate solubilizing bacteria are pseudomonas fluorescens or pseudomonas putida; the growth promoting bacteria are bacillus subtilis or bacillus licheniformis; the weight ratio of the nitrogen-fixing bacteria to the phosphate solubilizing bacteria to the growth-promoting bacteria is 2: (1-1.5): (1-1.5). By applying the microbial fertilizer and sowing the grass seeds, the physical properties, chemical properties and microbial properties of the soil are effectively improved.

The invention relates to a lactobacillus plantarum LAS3 strain and an application of the lactobacillus plantarum LAS3 strain, and belongs to the technical field of microorganisms. The lactobacillus plantarum LAS3 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number of the lactobacillus plantarum LAS3 is GDMCC NO.66440. The strain is separated, purified and screened from Guizhou sour fish, and experiments show that the strain has excellent pH tolerance and sodium chloride tolerance; fermentation liquor of the strain has a good antibacterial effect on multiple common pathogenic bacteria such as pseudomonas aeruginosa, burkholderia cepacia, pseudomonas putida, staphylococcus aureus, bacillus subtilis and candida albicans, and bacteriocin generated by the strain has excellent antibacterial and preservative effects and also has excellent thermal stability and pH stability. The antibacterial preservative can be used as an antibacterial preservative raw material, is applied to food, health care products or daily chemical products, and has wide application prospects and important conversion research values.

The invention discloses a recombinant strain for efficiently expressing xanthine oxidase as well as preparation and application of the recombinant strain, and belongs to the technical field of enzyme engineering. According to the recombinant strain, four kinds of recombinant Pseudomonas putida strains are designed, and the strains can be used for efficiently expressing xanthine oxidase. The recombinant strain has the advantages of high expression quantity, simple affinity purification process flow, high purification yield and low production cost, and the recombinant xanthine oxidase has the specific activity similar to that of xanthine oxidase sold in the market at present and is suitable for various applications. The recombinant strain can realize high-efficiency expression of xanthine oxidase, and high-efficiency expression of xanthine oxidase is realized in P.putida through a gene recombination technology; the expressions are induced by adding an inducer, so that the maximum expression quantity of the xanthine oxidase is realized.

The invention relates to the field of microbial agents, in particular to a micro-ecological restoration liquid composite microbial agent suitable for heavy metal contaminated soil and a biological bacterial fertilizer and a preparation method thereof. The micro-ecological restoration liquid complex microbial inoculant suitable for the heavy metal contaminated soil comprises nitrogen-fixing bacteria: azotobacter chroococcum ACCC 11104, phosphate solubilizing bacteria: pseudomonas fluorescens ACCC 03031, potassium solubilizing bacteria: bacillus mucilaginosus CCTCC KB20082790, growth-promoting bacteria: azotobacter braziliensis CCTCC AB 91001, bacillus subtilis CGMCC 17161, synergistic bacteria: trichoderma asperellum CGMCC 3.17461, and restoration bacteria: pseudomonas putida CGMCC 1.4533. The biological bacterial fertilizer for the heavy metal contaminated soil is prepared from the liquid complex microbial inoculant, compared with a conventional biological bacterial fertilizer, the adaptability to the heavy metal contaminated soil is greatly improved, the heavy metal contaminated soil is effectively improved, and the crop yield is remarkably increased.

The invention relates to the technical field of synthetic biology, in particular to a microbial cell factory for producing catechol. Pseudomonas putida without a pcaHG gene, a catA gene and a catA2 gene is used as a chassis bacterium, and exogenous AroY and / or kpdB genes are / is introduced into the chassis bacterium, or the expression of endogenous genes VanAB and PobA is improved. The lignin-derived monomer is efficiently converted into catechol through the strategy of cofactor regulation and key enzyme overexpression, the conversion rate reaches 98.5%, the highest yield is 14.1 mM, and compared with the previous 64% conversion rate, the conversion rate is greatly improved.

The invention relates to the technical field of microorganisms, in particular to growth-promoting pantoea TY-88 and application thereof in promotion of waxy corn seed germination and seedling growth, the preservation unit of the growth-promoting pantoea TY-88 is China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025945, and the preservation date is April 30, 2025. The growth-promoting pantoea TY-88 provided by the invention has better capabilities in the aspects of phosphate solubilization, nitrogen fixation, potassium solubilization and siderophore production; when the biological agent prepared from the growth-promoting pantoea is used for seed soaking treatment, waxy corn seed germination, seedling growth and development and substance accumulation of the overground part and the underground part can be remarkably promoted, and economic benefits are improved on the premise that the environment is not polluted.

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.

Disclosed herein are methods, compositions and systems useful for genetically engineering subcellular compartments such as OMVs for synthetic biology applications. In an embodiment, genetically engineered bacteria use OMVs to secrete compounds or proteins of interest extracellularly where the compounds or proteins of interest can be isolated from the growth media.