17 results about "Indigenous bacteria" patented technology

A phage composition and an use thereof in strengthening soil carbon fixation, said phage composition including three phages, namely, a polyvalent phage φYSZKP that attacks Klebsiella pneumoniae and Pseudomonas aeruginosa; a Bacillus cereus phage φYSZBA1; and a Bacillus cereus phage φYSZBA2. In the present invention, the phage composition is injected into the target soil, and an approach of strengthening soil carbon fixation by means of cooperation with the host bacteria through injection of AMGs from the phage is employed; at the same time, the phage has a function of regulating the soil microbial communities from bottom to top; a network of symbiosis with indigenous bacteria is further optimized, and the soil carbon fixation potential is improved. This technique is an environmental improvement technique that achieves a good carbon fixation effect, has a low price and is environment-friendly.

The invention belongs to the technical field of disease control in aquaculture, and discloses a microecological preparation based on rana nigromaculata intestinal symbiotic bacteria and a preparation method and application thereof, the name of the microecological preparation is Bacillus subtilis QDHYB001, the classification name of the microecological preparation is Bacillus amyloliquefaciens, the preservation number is CGMCC No.34735, the preservation date is China General Microbiological Culture Collection Center on June 3, 2025, the preservation number is CGMCC No.34735, the preservation number is CGMCC No.34735, the preservation unit is China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.34735. The medicine is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Through three core breakthroughs of directional screening of rana nigromaculata intestinal indigenous bacteria, innovation of an insect source culture medium and optimization of a compounding process, the efficiency of the microecological preparation is improved.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The invention relates to a soil conditioner based on rare earth tailings, waste biomass and microorganisms and a preparation method of the soil conditioner. The method comprises the following steps: separating and breeding rare earth mine indigenous bacteria capable of tolerating rare earth elements from rare earth mine soil, and separating and breeding efficient lignin degrading bacteria from a natural environment; then mixing rare earth mine indigenous bacteria and efficient lignin degrading bacteria according to a certain proportion, compounding and culturing to prepare a liquid microbial agent; and mixing the rare earth tailings to be treated, the waste biomass and the liquid microbial agent, composting and fermenting to finally obtain the soil conditioner capable of improving the physical and chemical properties of soil, improving the fertilizer efficiency of the soil and improving the quality of crops. The method simultaneously solves the environmental protection problem caused by massive accumulation of the rare earth tailings and the resource utilization problem of the waste biomass in China, has the advantages of simple process flow, low production cost and the like, and provides a brand new idea for efficient treatment and resource utilization of the rare earth tailings.

This invention discloses an integrated soil regeneration granule for indigenous bacteria activation and soil improvement, and its preparation method. The granule employs a two-component (A / B) layered structure, consisting of a disintegration trigger core, an A-component habitat layer, and a B-component functional layer from the inside out. The disintegration trigger core contains citric acid microcapsules coated with gelatin-gum arabic, which, in synergy with sodium bicarbonate, achieves precise gas production and disintegration over 12 months, propelling microbial communities and nutrients to deeper soil layers. The A-component habitat layer constructs a gradient porous structure, inhibiting pore blockage and efficiently capturing indigenous bacteria. The B-component functional layer relies on biochar and humic acid for nutrient supply, and incorporates oyster shell powder to construct a pH buffering system. This granule can both capture and colonize beneficial indigenous bacteria, and can also be artificially and directionally implanted with beneficial bacteria, serving as an excellent carrier for microbial fertilizers to enhance their efficacy. It achieves synergistic effects of saline-alkali soil improvement, microbial activation, and soil structure reconstruction, specifically addressing the shortcomings of similar technologies in Japan, and has a wider range of applications.

The invention relates to the technical field of a cloth net type microorganism synergistic degradation remediation method for pesticide residue soil, in particular to a cloth net type microorganism synergistic degradation remediation method for pesticide residue soil, which comprises the following steps: step 1, carrying out early-stage investigation and physical and chemical property analysis on polluted soil: firstly, investigating a target pesticide residue polluted soil area, and analyzing the physical and chemical properties of the polluted soil; determining the pollution range, the pollution degree and the types of pesticide residues; step 2, screening functional flora and constructing a synergistic degradation system; wherein the early investigation in the step 1 is accurate and clear in pollution information, a targeted basis is provided for subsequent repair, blindness of traditional repair is avoided, and the problem of microbial agent waste caused by extensive repair is solved. According to the multi-flora synergistic system constructed in the step 2, the environmental adaptability is improved through indigenous bacterium screening, the degradation spectrum is widened through flora metabolism complementation, and the defects that the degradation range of a single strain is narrow, and combined pollution is difficult to deal with are overcome.

A process includes identifying a hydrocarbon-bearing reservoir containing a hydrocarbon fluid and a brine having a sufficient salinity level to prevent or inhibit one or more of methanogenesis and sulphate-reduction activity, and responsive to a concentration of hydrogen being below 1000 parts per million in a sample collected from the hydrocarbon-bearing reservoir, introducing at least one non-native or indigenous hydrogen producing microorganism or stimulating the activity of at least one indigenous bacteria capable of stimulating a microbial consortium to increase a production rate of hydrogen in the hydrocarbon-bearing reservoir.

The invention relates to the technical field of biological fermentation, in particular to a method for synergistically fermenting distillers' grains by bacteria and enzymes. The viable count of the three functional bacteria provided by the invention can reach 107CFU / g or above after the three functional bacteria are cultured for 5 days in an environment with the temperature of 50-60 DEG C and the pH value of 3.0-4.0. In the environment, indigenous flora naturally carried by the yellow wine lees are difficult to survive, interference of indigenous bacteria can be basically eliminated in the fermentation process, growth and reproduction of functional bacteria are facilitated, and the stability of large-scale fermentation can be improved. The bacterial enzyme synergistic process activates the metabolic diversity of microorganisms through enzymolysis, the content of propionic acid and acetic acid is remarkably increased, meanwhile, succinic acid is kept stable, and the antibacterial activity and metabolic balance of an organic acid spectrum can be optimized. Meanwhile, enzymolysis can promote degradation of medium-chain fatty acid, and enzymolysis continuously optimizes a lipid metabolism structure in the later stage. The proline content of a bacterium-enzyme synergistic fermentation group is far higher than that of a pure fermentation group, so that the palatability of the feed is remarkably improved. Moreover, methionine is kept stable in a bacterium-enzyme synergistic group, so that nutrition loss can be avoided.

The invention provides a method for preparing a carbon source by reinforcing kitchen waste based on a Fe composite MXene material. According to the method, the Fe composite MXene material is added in the anaerobic fermentation process of the kitchen waste, so that the external electron transfer capability of indigenous bacteria of the kitchen waste can be enhanced by utilizing Fe (III) sites on the surface of the material, the decomposition effect of organic matters in the kitchen waste is improved, and the quality of an organic biological carbon source is improved. Fe (III) on the surface of the material can be reduced by microorganisms to generate Fe (II), and the Fe (II) is oxidized into Fe (III) again when the Fe (II) is in contact with air, so that cyclic utilization of Fe (III) / Fe (II) is realized. The Fe composite MXene material can replace a traditional functional microbial agent and organic matter decomposing enzyme, the fermentation period of the kitchen waste is shortened, the solubility COD of the organic biological carbon source and the volatile organic acid carbon source are improved, and the method has wide application prospects in the aspect of preparing the carbon source from the kitchen waste.

A strain of bacteria, HITLi7, based on the quantification of conserved gene abundance. T A rapid and specific detection method is involved, which uses the determination of the abundance of species-specific conserved genes to quickly detect HITLi7. T This invention addresses the challenges of complex microbial composition and the limited availability of HITLi7 strains in current bio-enhanced activated carbon processes. T The technical challenge of rapidly and specifically identifying and detecting abundance changes in indigenous bacteria presents a problem. This invention utilizes the double-positive reaction of the bispecific conserved genes gyrB and rpoB to further enhance the specificity of the detection results. The high sensitivity and accuracy based on qPCR technology further strengthen the reliability of the results. The detection method of this invention provides highly targeted results, avoiding the large amount of redundant data and complex processing procedures associated with high-throughput technologies. Compared to detection methods based on traditional bacterial morphology and physicochemical properties, it has wider applicability, higher sensitivity, and greater accuracy.

The invention discloses a development method and application of soil indigenous bacteria. The development method of the soil indigenous bacteria comprises the following steps: collecting soil; the method comprises the following steps: preparing a soil suspension from soil and water, inoculating the soil suspension into a culture medium by a gradient dilution method, and separating single colony microorganisms to obtain a plurality of strains; the method comprises the following steps: screening strains which produce indoleacetic acid, produce microbial siderophores, solubilize phosphorus, solubilize potassium and / or have antagonism with soil-borne pathogenic bacteria from various strains to obtain functional strains; bacterial strains with symbiotic and / or synergistic effects are selected from the functional bacterial strains in a mixed culture mode to be compounded, and a composite flora is obtained; single strains in the composite flora or the functional strains are used for soil improvement; the method for developing the indigenous bacteria in the soil has no requirement on the area where the soil is located, the indigenous bacteria can be developed for the soil in any area, the indigenous bacteria highly synergetic with the local soil ecology can be obtained, and the application effect is improved.

The invention belongs to the technical field of water treatment, and particularly relates to a method for rapidly degrading refractory soluble organic matters in lake water by using a microbial agent. Through the synergistic effect of aerobic bacteria and anaerobic bacteria, the adsorption and catalysis functions of a catalyst carrier and mutual cooperation of biodegradation and catalytic oxidation, the metabolic limitation of traditional microorganisms on refractory organic matters such as benzene rings and long-chain hydrocarbons is broken through, efficient removal of the refractory organic matters in a water body is achieved, and the water quality is improved. The multi-stage porous structure rapidly adsorbs and enriches pollutants, the catalytic oxidation effect of the loaded metal oxide weakens the organic matter structure, meanwhile, the degradation effect of indigenous bacteria achieves thorough mineralization, the multi-step synergistic effect greatly improves the degradation efficiency, the concentration of refractory organics in a water body is remarkably reduced, and then the water quality is improved.

The application discloses micro-polluted water smell targeting composite biological filter material and preparation and application thereof, the filter material is a spherical particle, and sequentially comprises a high-density manganese sand core accounting for 75% of the diameter, an amino-chitosan biological affinity layer accounting for 15%-20%, and a COF-graphene hybrid adsorption layer accounting for 5%-10%; the three-layer structure cooperatively realizes selective adsorption, electrochemical activation and biological mineralization of geosmin, 2-MIB and other odor-causing substances; the outer layer is rich in easily degradable nutrients for metabolism of the middle-layer indigenous bacteria, the inner layer delta-MnO2 and graphene construct an electron transfer channel, and oxidation degradation is strengthened; the filter material has excellent sedimentation, mechanical strength and self-sustaining ecological functions, and significantly improves long-term, stability and impact resistance of smell removal, and is suitable for deep treatment of drinking water.

The present application relates to black soldier fly breeding, especially a black soldier fly breeding method; a plurality of portions of soil at a breeding site and a plurality of portions of sterile manure are randomly taken, an equal amount of sterile manure is added to each portion of soil, and stirring is performed to obtain a mixture; qualitative analysis is performed on the mixture to determine whether the mixture contains protease and lipase, and if so, the mixture is retained, and if not, the mixture is discarded; the retained mixture is inoculation slurry; each inoculation slurry is mixed with corresponding sterile manure at a weight ratio of 1:90, and fermentation is performed for 10 days to obtain pre-prepared feed; black soldier fly larvae are inoculated into each pre-prepared feed, and cultivation is performed for 10 days; the optimal pre-prepared feed is comprehensively evaluated according to three indexes of the survival number of black soldier fly larvae, the weight of the larvae, and crude protein, and the corresponding inoculation slurry of the optimal pre-prepared feed is used as fermentation bacteria for amplification; indigenous bacteria in the soil at the breeding site are selected and bred to construct fermentation bacteria, and fermentation bacteria with the functions of decomposing protein and fat are obtained through screening as the final production bacteria, so that the risk of black soldier fly breeding is reduced, and the quality of black soldier fly products is improved.

The present application belongs to the technical field of water treatment, and particularly relates to a method for rapidly degrading refractory dissolved organic matter in lake water by using microbial agents. The present application breaks through the metabolic limitation of traditional microorganisms on refractory organic matter such as benzene ring and long-chain hydrocarbon by the synergistic effect of aerobic and anaerobic bacteria, the adsorption and catalytic function of catalyst carriers, the mutual cooperation of biodegradation and catalytic oxidation, realizes the efficient removal of refractory organic matter in water, rapidly adsorbs and enriches pollutants by the multi-stage pore structure, weakens the structure of organic matter by the catalytic oxidation of the loaded metal oxides, and realizes complete mineralization by the degradation of indigenous bacteria, so that the degradation efficiency is greatly improved, the concentration of refractory organic matter in water is significantly reduced, and the water quality is improved.

The invention relates to the technical field of water treatment, and particularly discloses a slow-release agent for stimulating urease-producing bacteria to repair underground water and a preparation method of the slow-release agent. Coating the composite hydrogel; and constructing the tricalcium phosphate of the slow release layer. Through a three-layer sequential release mechanism, the tricalcium phosphate shell is rapidly dissolved to increase the pH and inhibit indigenous bacteria; a pectin-yeast extract intermediate layer slowly releases an exclusive carbon and nitrogen source to activate urease-producing bacteria; the nickel-doped hydroxyapatite core continuously provides a urease cofactor Ni < 2 + >, gradient freezing directional pore-forming and steam hydrate physical curing processes are combined, the activity of urease producing bacteria is greatly improved, the carbon and nitrogen release period is prolonged, indigenous bacteria competition is remarkably inhibited, and the risk of heavy metal pollution is eliminated through lattice bound nickel; the validity period of the medicament is greatly prolonged.

The present application relates to a kind of biological enzyme strengthening liquid and its preparation method and application, by weight parts, include the following components: crude glycerol 10-40 parts, soybean meal 3-6 parts, corn flour 3-6 parts, yeast fermentation liquor 5-40 parts, inorganic composite carrier 1-5 parts, composite biological bacteria 0.5-2 parts, composite biological enzyme 0.1-1 part and water 10-50 parts;The biological enzyme strengthening liquid of the present application is by a large number of glyceric acid small molecules homogenization carbon source and nutrient factor, and the co-metabolic degradation effect of microorganism is strengthened, with corresponding long-chain alkane degrading bacteria and extracellular enzyme, the decomposition of substrate pollutants is strengthened, trace element and electron donor activate the activity of indigenous bacteria, quickly form the symbiotic, metabolic cooperation of specific flora system, multi-collaboration, and then enhance the system resistance to impact load capacity, the COD degradation effect of oily wastewater is remarkable.