66 results about "Indigenous bacteria" patented technology

The invention relates to a method for preparing an enzyme and bacterium composite agent for treating sewage and sludge, which comprises the following steps: (1) preparing bacillus subtilis powder; (2) preparing bacterial solution of photosynthetic bacteria; (3) obtaining indigenous bacteria by collection, culture and enrichment culture; (4) performing domestication culture of working bacteria, namely adding indigenous bacteria and a composite culture medium according to different sludge loads, adding bacillus subtilis enzyme powder in an amount which is 0.1 to 0.4 percent based on total weight and bacterial solution of photosynthetic bacteria in an amount which is 1 to 3 percent based on the total weight, performing tests under different loads and different organic matter concentration, determining an optimal parameter, and repeating for 3 times; and (5) performing domestication and amplification culture of the working bacterium composite agent, and obtaining the domesticated working bacterium composite agent in which the viable count per milliliter is cfu 10<7> to 10<9>. The enzyme and bacterium composite agent prepared by the method can treat sewage and sludge efficiency, simplyand conveniently, has very high adaptability to any sewage environment, and has a guiding and coordinating effect on beneficial decomposing bacteria in local environments.

The invention relates to a method for preparing a microbial fertilizer, a nutrient solution, a fodder and a pesticide and for fermentation bed cultivation by utilizing indigenous microorganism probiotics. The microorganism probiotics are formed by fermenting eight pure natural materials, including indigenous bacteria, green juice, fresh fish amino acid, enzyme alcohol, Chinese prescription nutrient solution, lactic acid bacteria, natural calcium and water-soluble calcium phosphate potassium; the microorganism probiotics contain the mixed flock of various beneficial bacteria, such as, lactic acid bacteria, photosynthetic bacteria, bacillus and yeast; the fertilizer can be used for fertilizing and loosening soil; the agricultural products are free from residue, taste nice and are high in yield; the fodder can be used for guaranteeing no residue on the cultured animal meat product; the meat is tender and free from smell; after the microorganism nutrient solution is used, the microorganism pesticide can prevent and treat insect diseases; the chemical pesticide and the fertilizer residue can be eliminated and decomposed; when the fermentation bed is used for culturing, the excrement cleaning is not required; the animal excrement is decomposed by the fermented padding while beneficial mycoprotein is formed; the fodder can be eaten by the animals; the microbial fertilizer is odorless, zero-emission, environment-friendly, pollution-free to environment and beneficial to the health of human and animals.

The invention relates to a preparation method of aerobic high-temperature static compost and discloses a preparation method of the aerobic high-temperature static compost by using corrosion-promoting compost composite fermentation bacteria inoculants. The preparation method includes that an indigenous microorganism strain indigenous bacteria agent and thermophilic bacteria thermophilic fat bacilli are combined to be prepared into composite microbial inoculants, temperature of a pile rises rapidly more than 55 DEG C in early access of a compost substrate, previous time of pile fermentation is shortened, activity of substrate microorganism is enhanced, and the compost enters a high-temperature stage in a shortest time. Through activity of thermophilic bacteria and oxygen leading in the pile, rapid decomposition is achieved, and therefore compost quality is improved.

A method of using indigenous bacteria to restore oil-contaminated soil is to add mixed indigenous bacteria screened and enriched from oil-contaminated soil to oil-contaminated soil for bioremediation, and the specific steps include: oil-contaminated soil Grinding and sieving pretreatment, using inorganic salt medium to screen and enrich indigenous bacteria from oil-contaminated soil, adding enriched indigenous bacteria to oil-contaminated soil, and adding a certain proportion of inorganic nutrients to oil-contaminated soil Compost after stirring evenly, and plow and water the treated soil regularly. The method can be used for both in-situ remediation and ex-situ remediation of oil-contaminated soil, and can be used for remediation of high-concentration petroleum-contaminated soil and low-concentration petroleum-contaminated soil, and the medicines used are easy to obtain and easy to operate. And the degradation efficiency is high, and will not cause secondary pollution.

The invention discloses a pig feed additive and a preparation method thereof, belongs to the technical field of feeds, and solves the problems that the existing feed additive is single in raw material and relatively low in effect. The pig feed additive is formed by combining fermentation products with liquid water contents of smaller than or equal to 16wt% obtained by respectively fermenting bacteria liquids of 18 parts of aspergilli group, 18 parts of microzyme group, 30 parts of bacillus group and 40 parts of complex bacteria group in parts by weight. The preparation method comprises the following steps of: preparing a culture medium; cooling; diluting indigenous bacteria; inoculating; fermenting; drying; mixing; and packaging. The feed additive disclosed by the invention contains 20 types of probiotics, and has the effects of adjusting pig intestinal flora balance, controlling intestinal diseases, improving immunity, enhancing digestion function and improving feed conversion rate.

The invention provides a method for increasing the coal bed gas yield through indigenous bacteria. The method comprises the steps of (1) a methanogens community is enriched and cultured, specifically, a coal sample and a water sample are collected from a target zone, enrichment culture and fermentation of the methanogens community are conducted, and fermentation liquor is obtained; (2) ultrasonic coupling supercritical CO2 treatment is conducted, specifically, a laboratory operation and maintenance management system is used, the optimal condition for ultrasonic coupling supercritical CO2 treatment on the coal seam sample is analyzed in a laboratory, and a target coal seam is treated under the optimal condition; and (3) coal degradation through microorganism is conducted, specifically, the fermentation liquor obtained in the step (1) is injected into the target coal seam, changes of injection wellhead gas components and the methane concentration are detected continuously, and methane is gathered in drainage, pressure lowering and recovering modes. The treatment time is effectively shortened through the ultrasonic coupling supercritical CO2 treatment, the coal bed gas generation speed is increased, and meanwhile the working efficiency is effectively improved through the laboratory operation and maintenance management system.

The invention belongs to the field of the petroleum industry, and particularly relates to a sustained-release long-acting nutrient for improving the valid period of indigenous microbial oil recovery. The invention adopts the technical principle that the conventional nutrient is prepared from water-soluble raw materials such as diammonium hydrogen phosphate, urea, ammonium nitrate, sodium nitrate, amino acid, molasses and the like. The raw materials are water-soluble, have different adsorption capacities in a stratum and different moving speeds, are easily drained and lost, and have short valid period. The nutrient is immobilized by adopting an immobilization method with hydrogel and the like and slowly released in the stratum; and organic phosphate, low molecular weight protein powder and the like are slowly hydrolyzed in the stratum to generate the nutrient required by indigenous bacteria. The using concentration of the sustained-release nutrient is 0.2 to 2.0 percent. The valid period of indigenous bacteria oil recovery is prolonged, so that the nutrient can enter the deep part of an oil deposit, and the efficiency of the indigenous microbial oil recovery is improved.

The invention relates to a preparing method of microbial inoculants and discloses a preparing method of decomposition promoting and composting compound fermentation inoculants. The preparing method of decomposition promoting and composting compound fermentation inoculants includes: collecting indigenous bacteria, culturing the indigenous bacteria in enlarge cultivation mode, culturing the solid inclined surface of bacillus stearothermophilus, culturing the bacillus stearothermophilus in enlarge cultivation mode, culturing the bacillus stearothermophilus in a fermentation tank in aerobic culture mode, mixing the obtained solid indigenous bacteria inoculants and the obtained bacillus stearothermophilus solid inoculants in weight percent of (2-3):1 to prepare the decomposition promoting and composting compound fermentation inoculants. The obtained decomposition promoting and composting compound fermentation inoculants are fertilized in composting substrate at the early stage, can fast improve pile temperature and reduce pile PH value, shortens pile fermentation earlier-stage time, promotes substrate microorganism movement, leads composts to enter the high temperature stage in the shortest time, achieves fast thorough decomposition through thermophilic bacteria movement and oxygen filling in stacked piles, and accordingly improves composting quality.

A process for biological restoration of ground water in a mineralized sandstone formation using a nutrient source. The process is used in conjunction with mining uranium using in-situ pump and treat technology. The restoration of the ground water is designed to meet federal and state regulations. The process includes pumping the contaminated ground water from the mineralized sandstone formation using production wells to the ground surface. The ground water is then circulated through at least one ion exchange column. The ion exchange column is used to capture any residual uranium remaining in solution. The treated ground water then flows from the ion exchange column through at least one reverse osmosus unit. The reverse osmosus unit is designed to help decrease any remaining dissolved solids in solution, such as sodium and chloride. From the reverse osmosus unit, the ground water is circulated through at least one de-carbonation column. The de-carbonation column is used to remove residual carbon dioxide in the ground water. Upon exiting the de-carbonation column, a nutrient source is introduced into the ground water for stimulating the indigenous bacteria in the mineralized sandstone formation. The ground water is now pumped back into the ground where it is reintroduced into the sandstone formation.

Recalcitrant chemical oxygen demand (COD) of a liquid is reduced in a water treatment system. The method includes pretreating the liquid in a pretreatment unit to remove indigenous bacteria or microbes to a population level below which the indigenous organisms can interfere with the screened and externally introduced microorganisms. The liquid is then provided to a reactor that has a filter bed formed with a carrier material. Special microbes are screened and used to colonize the carrier material to remove recalcitrant COD. A biofilm is cultured on the surface of the carrier material to immobilize the screened microbes in the reactor. The method further includes percolating the liquid from the pretreatment unit through the filter bed colonized with the screened microbes to degrade at least part of the recalcitrant COD under aerobic conditions. In one embodiment, the filter is formed with biological granular activated carbon (GAC) as the carrier material and the screened microbes comprise at least one microbial species selected from the group consisting of Bacillus, Comamonas, Arthrobacter, Micrococcus, Pseudomonas, Pediococcus, Achromobacter, Flavobacterium, Mycobacterium, Rhodanobacter, Stenotrophomonas and Yeast.

The invention relates to a comprehensive treatment method for repairing the in-situ biological diversity in rivers and lakes, which is characterized in that the bottom sediments in water areas are quickly purified by taking indigenous bacteria from the rives and the lakes and regulating the pH value; the colonization of the original zoological biological diversity is strengthened by utilizing the nutrition preparation of a PU (Polyurethane) hydrolysis bubble film to degrade organic pollutants. The invention has the advantages that foreign ecological strains are not introduced, thus no variety appears, no secondary or micro-pollution nuisance appears; an automatic aerator is not arranged and sludge is not cleaned, thus the operation and maintenance cost and the investment of equipment are greatly reduced, the effect is obvious and good; and the cannel drainage function and the natural purification function are not influenced in the course of treatment and after the treatment.

The invention relates to the field of biological fertilizers, in particular to a high-quality biological fertilizer and a preparation method thereof. According to the invention, a secondary fermentation method and an indigenous bacteria screening application method are applied innovatively, and a safe, efficient and convenient production method is provided, a microbial environment in which harmonious symbiosis of microbial flora is implemented and indigenous bacteria are dominant flora is obtained, and continuous accumulation of microbial metabolites is promoted. The nutritional value of the biological fertilizer is increased to achieve the following three purposes including, harmlessness, humification and generation of a large number of microorganisms and metabolites of the microorganismssuch as various antibiotics and protein substances.

The invention discloses a preparation method of an organic fertilizer for ecological restoration. The preparation method is prepared from the following raw materials in parts by weight: 5-8 parts of wheat straws, 5-8 parts of straws, 5-7 parts of tree barks, 5-7 parts of wood chip, 10-25 parts of excrement, 1-5 parts of modified silicate, 15-28 parts of fertile soil, 5-7 parts of microorganism bacteria and 1-5 parts of earthworms, wherein silicate is modified laminar silicate mineral powder, the fertile soil is black soil containing nitrogen phosphorus and potassium, and the microorganism bacteria is aboriginal bacteria, non-indigenous bacteria or genetically engineered bacteria. The utilized fertilizer is a low-cost material, the resources are easily available, and a large number of recycled and abandoned resources are used, so that great benefit is created when the nutriments of the fertilizer are guaranteed; and the soil structure is not damaged by the organic fertilizer, the hardening of the soil is not caused, the organic fertilizer has good nourishment protecting protectability to soil, and the natural ecological balance is prolonged.

The invention provides a sewage treatment agent and a preparation method thereof and belongs to the technical field of sewage treatment. The sewage treatment agent is composed of polyacrylamide, citric acid, corn starch, coke slag powder, activated carbon, sodium alginate, lactic acid bacteria, actinomycetes, saccharomycetes, bifidobacteria, photosynthetic bacteria, bacillus and indigenous bacteria. The sewage treatment agent has a fast reaction rate, does not produce toxic substances during the reaction process, realizes ecological and resource sewage treatment, is free of a sludge treatmentsystem, prevents secondary pollution and has stable treatment effects.

The invention relates to a method for preparing a microbial preparation for rotting crop straw. The microbial flora used is named WH flora, which belongs to the genus Bacillus. Filter mud is used as the three-stage medium and carrier of WH flora. Group preparation, secondary strain cultivation, tertiary strain cultivation and other process steps are used to make rotten agent. The degradation products of WH flora provide energy and nutrition to native cellulolytic bacteria and various spoilage flora, enabling them to rapidly activate and cooperate with WH flora to rapidly decay crop straws. The method has the advantages of simple process, low cost, high number of live bacteria, easy use and easy popularization and application in a large area.

The present invention relates to the technical field of aquaculture and particularly relates to a preparation method of a palatability-type insect powder feed for fish. Firstly, protein and various nutrient-enriched kitchen wastes are used as culture materials, the culture materials and lactic acid bacteria are mixed, the mixture is subjected to stack fermentation, and stacking is conducted at anoptimum temperature of the lactic acid bacteria, so that the lactic acid bacteria compete with other indigenous bacteria species in the kitchen wastes, the lactic acid bacteria become an advantage population in the kitchen wastes, smell-unpleasant substances produced by fermentation of the indigenous bacteria species are reduced, and thus a problem of poor self-smell of hermetia illucens caused bytaking the kitchen wastes with the smell-unpleasant substances is fundamentally avoided. In addition, the lactic acid bacteria are as a probiotic, can promote digestion of the hermetia illucens, shorten time of nutrient conversion, and improve production efficiency of the feed. In addition, an addition of the lactic acid bacteria can produce lactic acid and the production of the lactic acid can improve feed palatability.

The invention relates to indigenous bacterium blocks for treating dystrophication, which are characterized in that: the indigenous bacterium blocks in various shapes are prepared by mixing and uniformly stirring 61 to 65 weight percent of indigenous bacteria culture mud with the water content of 48 to 55 percent, 23 to 28 weight percent of calcium hydroxide Ca(OH)2, 2 to 3 weight percent of coarse granulated sugar, 1.5 to 3 weight percent of calcium peroxide CaO2 and 4 to 5.5 weight percent of glutinous rice paste, feeding the mixture into a mold and pressing, wherein the indigenous bacteria culture mud is prepared by the following steps of: extracting a river lake substrate sludge sample and separating to obtain required indigenous bacteria, namely anaerobic and facultative strains; multiplying the indigenous bacteria; and anaerobically adjusting to make the indigenous bacteria in a dormant state. The indigenous bacterium blocks are easy to transport and throw; and when the indigenous bacterium blocks are thrown into rivers and lakes, the indigenous bacteria in the indigenous bacterium blocks quickly multiply in the rivers and lakes and decompose organic pollutants so as to eliminate darkening and odor of the rivers and lakes, which is a means for treating pollution by using waste.

The invention discloses cat litter capable of biodegrading cat excreta. The cat litter is prepared from, by weight, 900-1,000 parts of plant scraps, 18-22 parts of corn flour and 180-220 parts of fermented brown sugar glutinous rice, wherein indigenous bacteria are composed of yeast, filamentous fungi, lactic acid bacteria, actinomycetes and bacillus. All the components in a whole formula of the cat litter are matched with one another to mutually play a synergistic role; the prepared wood dust particle cat litter and the prepared straw particle cat litter, both of which contain the indigenousbacteria and can biodegrade cat excreta, are obvious in deodorization effect and long in service life and do not require frequent cleaning. Through the synergistic fermentation effect of padding and cat excreta, cat excreta waste is quickly converted, malodor is eliminated, and germs are inhibited. The used cat litter can also be used as organic fertilizer for planting flowers and has high safetyand great environmental protection performance. The indigenous bacteria in the cat litter can inhibit the growth of harmful germs in the biological fermentation process and prevent massive harmful germs from breeding in the use process of the cat litter and threatening the health of pets, and the practicability is high.

The invention provides a live pig breeding fermentation bed technology which comprises: mixing raw materials, including 85-90% by weight of original sawdust, 10-15% by weight of unpolluted local soil and 0.5% by weight of large-grain original sea salt, with water to obtain bedding with the relative humidity of 60-70%; adding 0.7Kg per square meter of an indigenous bacteria strain and carrying out fermentation for 7-10 days; immediately adding the original sawdust when the bed surface descends by 25-35 cm, spraying a nutrient solution with the concentration of 0.1% when the relative humidity is less than 60%, and when the pig manure is accumulated, immediately scattering and dispersing pig manure or uniformly mixing the pig manure with the bedding and burying the mixed material into middle and lower layers of the bedding; after pigs are output, overturning and stacking the bedding, covering the bedding with a plastic film and carrying out fermentation for 7-10 days, then adding 0.7Kg per square meter of the indigenous bacteria strain and the nutrient solution with the concentration of 0.1% for adjusting the relative humidity to be 60-70%, and carrying out fermentation for 7-10 days for repeated use. The technology has the beneficial effects that for the bed which is used for 5 years, the problem of rotten and dead beds is avoided.

The invention relates to a process for manufacturing organic fertilizer from organic wastes. In the process, urban sludge and excrement of livestock and poultry are used as raw materials, and crushed straw plants and additives such as indigenous bacteria, filamentous bacteria, bacteria, actinomycetes, lactic acid bacteria, saccharomycetes and the like are added to manufacture the organic fertilizer. By using the organic fertilizer manufactured by the invention, the fertilizer investment can be reduced, the rural environment can be protected, a good agricultural ecosystem can be created, and the purposes of fertilizing soil, realizing stable and high yield and increasing yield and income can be achieved.

The invention discloses a soil microbial regulator for a wheat-maize crop rotation field and a preparation method of the soil microbial regulator. The soil microbial regulator is prepared from the following raw materials in parts by weight: 15-25 parts of amino acid raw powder, 5-10 parts of 100,000 U/g cellulase, 10-22 parts of bacillus subtilis, 10-22 parts of bacillus amyloliquefaciens, 5-15 parts of saccharomycetes, 5-15 parts of trichoderma viride and 5-10 parts of beauveria bassiana. The soil microbial regulator can give consideration to stimulation of soil indigenous bacteria, stimulaterapid propagation of the indigenous bacteria and achieve the purpose of original ecology. The soil microbial regulator is used for soil of wheat planted after maize straw is returned to the field, the tillering number of the wheat can be increased to a remarkable level, and the thousand seed weight of the wheat can be increased to be close to the remarkable level.

The invention discloses a microbiological inoculation device and a method for treating micro-polluted water by the microbiological inoculation device, and relates to the technical field of microbiological inoculation. The microbiological inoculation device comprises an ozone aeration tower, an industrial ozone generator, a porous ceramic catalyst unit, an ultraviolet catalyst unit, an inoculant tank, a culture solution tank, an inoculation biology filtration column, an air bottle and a biological filter chamber, an aeration tray is arranged at the inner bottom of the ozone aeration tower, the industrial ozone generator is communicated with the aeration tray in the ozone aeration tower, an outlet in the bottom of the ozone aeration tower is communicated with an inlet of the porous ceramic catalyst unit, an outlet of the porous ceramic catalyst unit is communicated with an inlet of the ultraviolet catalyst unit, and an outlet of the ultraviolet catalyst unit is communicated with a water pump I. The method includes the steps: firstly, ozone ultraviolet catalysis; second, bacterial inoculation and biodegradation treatment. The method solves the problems that nutrient substances in the micro-polluted water are insufficient, inoculated bacteria are easily affected by indigenous bacteria, and the method is used for treating the micro-polluted water.